AT3G18770

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005035
Physical Location & Seq
Reverse (-)
6459816 .. 6463101
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18770.1

Sequence Viewer

Length: 1878 bp
ATGTCATCTTCACACAACAGGAGTAACAACAACAATTCAGAAGGAGCTAAAGCAGAACAGATCATATTTGAGTTCTTTGCTAAAAGCCTTCACATCATTCTCGAATCAAGGACTCCTTTTATGTCTTCAAGGAACTTTAGTGGTGAGCAGATGATTTGTTCTCCTTCCTCATCTTCTTCCTCTTCCTCGAGTGTTAGACCTCGAGACAAATGGTTCAATCTAGCTCTTAGGGAATGTCCTGCAGCTTTAGAGAGTTTCGATATCGGGCGGAGAAGCAGTCTTGAGCCTTTGGTTGTTGATGTTGTTCTTGTGGTGAGGCCACTTGTTGGTGATCAGTCTGGTAAAAGGGAGCTTATCAGGAACTTCTCCGGGAAAGATTATCAGTCTGGTTGGAATTCTGATCAAGATGAATTGGGATGTGAGACTAAGAACGAGCAGATTATTGAGAGATGGGTGGTGCAATATGATAATAGGAAGATCAGGGAATCTGTTACTACTAGTAGCAGGAGGTCAAGCAGTAATAAGTTGCAAGTGATGTATAAGAAAGCTACTCTGCTGCTTAGATCATTGTTTGTCATGGTTAGGCTTTTACCTGCTTATAAGATTTTTCGGGAACTCAATTCCTCGGGGCAGATCTTCAAGTTCAAGCTTGTTCCTAGGGTCCCTTCTATAGTTGAGCCATTCACTCGTAAAGAAGAAGCAGAAATGCAGAAGTTTTCTTTCACACCGGTTGAAACGATCTGTGGTAGACTTTGCTTATCGGTTTTATATCGTTCTCTCTCAGATGTCAGTTGCGAACACTCTACACCTATGTCTCCAACATTTATAACCGATTATGTTGGAAGTCCCTTGGCTGATCCTTTAAAGAGGTTCCCTTCCCTTCCTCTTTCATATGGTTCTCCTCCATTGCTGCCATTTCAAAGGCGCCATAGCTGGAGTTTCGATCGTTACAAGGCATCTCCACCTTCTGTTTCTTGCTCACCATCGCCTACACGCTCGGATTCACATGCATTGGTTTCACATCCTTGCTCTCGTCATCTTCCTCCTCACCCTTCTGATATACCCACTGGTCGTAGGAAGGAAAGCTACCCCGAAGAGTATTCTCCTTGTCAAGATTTCTCCCCTCCTCCTTCTCCTTCAGCACCAAAACACGCCGTCCCAAGAGGCATCACACGTACTGAAAGTGCTCCAGTCAGAATACCAGCTCCAACTTTCCAGTCCAAAGAAAACGTTGTGGCACCTTCTGCTCATCTGAAGTTGTCGAGACACGCTTCGTTGAAGCCTGTGAGAAACTTGGGTCCTGGTGAATCTGGAGCAGCAATAGATAAGCTGTTTCTTTATGGGAGAGATGACTTTAGAAGACCTTCTGGGGTGAGGCCGTCTTCCAGCAGTTCTCCGCGGATATCATTTTCCCGGAGTTCCAGTAGATCTTTCCAAGACGATTTTGATGATCCTGATTTCCCTTGCCCATTTGATGTTGAGTATGATGATATTACAGATCGGAATAGCAGACCAGGGTCGTTTGACCACAGAGGAGACATACATGAACCATTCGATTCAAGCGGGTCATACCCGAAGAAGTCCCAAGATGCTGCAGTTGGTGCCCTTGTGAGAATGCTGAAGAAAGCGCCACCTCTCAGACAAGATGTCTCTGAATCTTCCAGACCTGAAATTTGCTCGAACAACAACAAACCCGCAGGGGCTCATGAGATAGCAGTAGCGTCTATCACAGCCTCGGGGATTGCTCTGGCCTCAAAGACAACAGCTGATGCTTTGGAAGAGTTGAGGTCTTACAAAGAAATGAAGAATCATTTGTTGCTTGGTCAATCCACATCAAACCCTTCCTCGGTTACTATTACTTCACCCTTTGATGTTTGA

Protein Analysis

625

Amino Acids

69.45

Weight (kDa)

9.09

Isoelectric Point (pI)

76.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG13 PF10033 22 - 259 1.3e-40 Autophagy-related protein 13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 600, 827
Acc36I ACCTGC 1 cut(s) 601
AccB1I GGYRCC 3 cut(s) 924, 1237, 1601
AccB7I CCANNNNNTGG 1 cut(s) 326
AccI GTMKAC 1 cut(s) 748
AccII CGCG 1 cut(s) 1399
AciI CCGC 5 cut(s) 268, 1397, 1399, 1563, 1695
AclI AACGTT 1 cut(s) 1230
AclWI GGATC 2 cut(s) 851, 1445
AcsI RAATTY 2 cut(s) 394, 1671
AcuI CTGAAG 3 cut(s) 1122, 1274, 1640
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 1 cut(s) 1177
AfiI CCNNNNNNNGG 2 cut(s) 326, 1846
AflIII ACRYGT 1 cut(s) 1172
AgeI ACCGGT 1 cut(s) 727
AgsI TTSAA 8 cut(s) 129, 217, 640, 646, 734, 920, 1279, 1560
AhdI GACNNNNNGTC 1 cut(s) 1646
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 2 cut(s) 1300, 1513
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 6 cut(s) 198, 416, 819, 1258, 1530, 1654
AlwI GGATC 2 cut(s) 851, 1445
Ama87I CYCGRG 4 cut(s) 187, 201, 625, 1735
AoxI GGCC 3 cut(s) 317, 1376, 1749
ApeKI GCWGC 5 cut(s) 242, 556, 910, 1316, 1592
ApoI RAATTY 2 cut(s) 394, 1671
ArsI GACNNNNNNTTYG 2 cut(s) 1656, 1688
AsiGI ACCGGT 1 cut(s) 727
Asp700I GAANNNNTTC 1 cut(s) 1363
AspA2I CCTAGG 1 cut(s) 656
AspLEI GCGC 2 cut(s) 927, 1630
AspS9I GGNCC 2 cut(s) 661, 1298
AsuC2I CCSGG 2 cut(s) 370, 1414
AsuHPI GGTGA 8 cut(s) 155, 325, 341, 972, 1040, 1316, 1384, 1854
AvaI CYCGRG 4 cut(s) 187, 201, 625, 1735
AvaII GGWCC 2 cut(s) 661, 1298
AvrII CCTAGG 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 1606
BanI GGYRCC 3 cut(s) 924, 1237, 1601
BanII GRGCYC 1 cut(s) 1705
BarI GAAGNNNNNNTAC 2 cut(s) 1070, 1102
BbsI GAAGAC 3 cut(s) 117, 1366, 1374
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 5 cut(s) 254, 543, 897, 1328, 1579
BccI CCATC 2 cut(s) 444, 991
BceAI ACGGC 2 cut(s) 1139, 1363
BciT130I CCWGG 2 cut(s) 1302, 1515
BclI TGATCA 2 cut(s) 331, 400
BcnI CCSGG 2 cut(s) 370, 1414
BcoDI GTCTC 6 cut(s) 198, 416, 819, 1258, 1530, 1654
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 3 cut(s) 221, 498, 657
BfmI CTRYAG 3 cut(s) 240, 669, 1593
BfoI RGCGCY 2 cut(s) 928, 1631
BfuAI ACCTGC 1 cut(s) 601
BglII AGATCT 2 cut(s) 633, 1427
BisI GCNGC 5 cut(s) 243, 557, 911, 1317, 1593
BlnI CCTAGG 1 cut(s) 656
BlsI GCNGC 5 cut(s) 244, 558, 912, 1318, 1594
Bme1390I CCNGG 4 cut(s) 370, 1302, 1414, 1515
Bme18I GGWCC 2 cut(s) 661, 1298
BmeRI GACNNNNNGTC 1 cut(s) 1646
BmeT110I CYCGRG 4 cut(s) 187, 201, 625, 1735
BmgT120I GGNCC 2 cut(s) 661, 1298
BmiI GGNNCC 7 cut(s) 662, 663, 872, 926, 1239, 1299, 1603
BmrFI CCNGG 4 cut(s) 370, 1302, 1414, 1515
BmsI GCATC 4 cut(s) 965, 1176, 1579, 1759
BoxI GACNNNNGTC 1 cut(s) 1516
BpiI GAAGAC 3 cut(s) 117, 1366, 1374
BpmI CTGGAG 3 cut(s) 955, 1173, 1332
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 2 cut(s) 370, 1414
BsaAI YACGTR 1 cut(s) 1175
BsaHI GRCGYC 1 cut(s) 925
BsaJI CCNNGG 7 cut(s) 624, 656, 849, 1397, 1514, 1734, 1845
BsaWI WCCGGW 1 cut(s) 727
BsaXI ACNNNNNCTCC 4 cut(s) 262, 292, 1408, 1438
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 1846
Bse118I RCCGGY 1 cut(s) 727
Bse1I ACTGG 4 cut(s) 1072, 1190, 1216, 1422
Bse3DI GCAATG 1 cut(s) 905
BseBI CCWGG 2 cut(s) 1302, 1515
BseDI CCNNGG 7 cut(s) 624, 656, 849, 1397, 1514, 1734, 1845
BseGI GGATG 2 cut(s) 422, 1021
BseLI CCNNNNNNNGG 2 cut(s) 326, 1846
BseMI GCAATG 1 cut(s) 905
BseMII CTCAG 2 cut(s) 795, 1651
BseNI ACTGG 4 cut(s) 1072, 1190, 1216, 1422
BseRI GAGGAG 4 cut(s) 891, 1035, 1116, 1548
BseSI GKGCMC 1 cut(s) 1606
BseXI GCAGC 5 cut(s) 254, 543, 897, 1328, 1579
Bsh1236I CGCG 1 cut(s) 1399
Bsh1285I CGRYCG 1 cut(s) 946
BshFI GGCC 3 cut(s) 319, 1378, 1751
BshNI GGYRCC 3 cut(s) 924, 1237, 1601
BshTI ACCGGT 1 cut(s) 727
BsiEI CGRYCG 1 cut(s) 946
BsiHKAI GWGCWC 1 cut(s) 1189
BsiHKCI CYCGRG 4 cut(s) 187, 201, 625, 1735
BsiSI CCGG 3 cut(s) 369, 728, 1414
BslFI GGGAC 4 cut(s) 647, 831, 1142, 1567
BslI CCNNNNNNNGG 2 cut(s) 326, 1846
BsmAI GTCTC 6 cut(s) 198, 416, 819, 1258, 1530, 1654
BsmFI GGGAC 4 cut(s) 647, 831, 1142, 1567
BsmI GAATGC 1 cut(s) 1620
BsnI GGCC 3 cut(s) 319, 1378, 1751
BsoBI CYCGRG 4 cut(s) 187, 201, 625, 1735
Bsp1286I GDGCHC 3 cut(s) 1189, 1606, 1705
BspACI CCGC 5 cut(s) 268, 1397, 1399, 1563, 1695
BspANI GGCC 3 cut(s) 319, 1378, 1751
BspCNI CTCAG 2 cut(s) 794, 1650
BspFNI CGCG 1 cut(s) 1399
BspHI TCATGA 1 cut(s) 1705
BspLI GGNNCC 7 cut(s) 662, 663, 872, 926, 1239, 1299, 1603
BspMAI CTGCAG 2 cut(s) 244, 1597
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 2 cut(s) 851, 1445
BspT107I GGYRCC 3 cut(s) 924, 1237, 1601
BsrDI GCAATG 1 cut(s) 905
BsrFI RCCGGY 1 cut(s) 727
BsrI ACTGG 4 cut(s) 1072, 1190, 1216, 1422
BssAI RCCGGY 1 cut(s) 727
BssECI CCNNGG 7 cut(s) 624, 656, 849, 1397, 1514, 1734, 1845
BssNI GRCGYC 1 cut(s) 925
BssT1I CCWWGG 2 cut(s) 656, 849
Bst2UI CCWGG 2 cut(s) 1302, 1515
Bst6I CTCTTC 3 cut(s) 187, 1089, 1773
BstACI GRCGYC 1 cut(s) 925
BstAPI GCANNNNNTGC 2 cut(s) 1244, 1601
BstBAI YACGTR 1 cut(s) 1175
BstDEI CTNAG 5 cut(s) 227, 426, 560, 781, 1637
BstDSI CCRYGG 1 cut(s) 1397
BstF5I GGATG 2 cut(s) 422, 1021
BstFNI CGCG 1 cut(s) 1399
BstH2I RGCGCY 2 cut(s) 928, 1631
BstHHI GCGC 2 cut(s) 927, 1630
BstMAI GTCTC 6 cut(s) 198, 416, 819, 1258, 1530, 1654
BstMCI CGRYCG 1 cut(s) 946
BstMWI GCNNNNNNNGC 2 cut(s) 1244, 1601
BstNI CCWGG 2 cut(s) 1302, 1515
BstNSI RCATGY 1 cut(s) 1010
BstPAI GACNNNNGTC 1 cut(s) 1516
BstSCI CCNGG 4 cut(s) 368, 1300, 1412, 1513
BstSFI CTRYAG 3 cut(s) 240, 669, 1593
BstSLI GKGCMC 1 cut(s) 1606
BstUI CGCG 1 cut(s) 1399
BstV1I GCAGC 5 cut(s) 254, 543, 897, 1328, 1579
BstV2I GAAGAC 3 cut(s) 117, 1366, 1374
BstX2I RGATCY 2 cut(s) 633, 1427
BstYI RGATCY 2 cut(s) 633, 1427
BsuRI GGCC 3 cut(s) 319, 1378, 1751
BtgI CCRYGG 1 cut(s) 1397
BtgZI GCGATG 1 cut(s) 969
BtsCI GGATG 2 cut(s) 422, 1021
BtsIMutI CAGTG 1 cut(s) 1065
BveI ACCTGC 1 cut(s) 601
CciI TCATGA 1 cut(s) 1705
CfoI GCGC 2 cut(s) 927, 1630
Cfr10I RCCGGY 1 cut(s) 727
Cfr13I GGNCC 2 cut(s) 661, 1298
Cfr42I CCGCGG 1 cut(s) 1400
CseI GACGC 1 cut(s) 1710
Csp6I GTAC 1 cut(s) 1176
CspAI ACCGGT 1 cut(s) 727
CviAII CATG 4 cut(s) 577, 1007, 1544, 1706
CviQI GTAC 1 cut(s) 1176
DdeI CTNAG 5 cut(s) 227, 426, 560, 781, 1637
DinI GGCGCC 1 cut(s) 926
DraI TTTAAA 1 cut(s) 864
DriI GACNNNNNGTC 1 cut(s) 1646
Eam1104I CTCTTC 3 cut(s) 187, 1089, 1773
Eam1105I GACNNNNNGTC 1 cut(s) 1646
EarI CTCTTC 3 cut(s) 187, 1089, 1773
EciI GGCGGA 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 656, 849
Eco24I GRGCYC 1 cut(s) 1705
Eco32I GATATC 2 cut(s) 262, 1404
Eco47I GGWCC 2 cut(s) 661, 1298
Eco57I CTGAAG 3 cut(s) 1122, 1274, 1640
Eco88I CYCGRG 4 cut(s) 187, 201, 625, 1735
EcoO109I RGGNCCY 2 cut(s) 661, 1298
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 1300, 1513
EcoRV GATATC 2 cut(s) 262, 1404
EcoT14I CCWWGG 2 cut(s) 656, 849
EcoT22I ATGCAT 1 cut(s) 1012
EcoT38I GRGCYC 1 cut(s) 1705
EgeI GGCGCC 1 cut(s) 926
EheI GGCGCC 1 cut(s) 926
ErhI CCWWGG 2 cut(s) 656, 849
FaeI CATG 4 cut(s) 580, 1010, 1547, 1709
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 4 cut(s) 647, 831, 1142, 1567
FatI CATG 4 cut(s) 576, 1006, 1543, 1705
FauI CCCGC 2 cut(s) 1556, 1702
FauNDI CATATG 1 cut(s) 892
FbaI TGATCA 2 cut(s) 331, 400
FblI GTMKAC 1 cut(s) 748
Fnu4HI GCNGC 5 cut(s) 243, 557, 911, 1317, 1593
FokI GGATG 2 cut(s) 429, 1008
FriOI GRGCYC 1 cut(s) 1705
Fsp4HI GCNGC 5 cut(s) 243, 557, 911, 1317, 1593
FspBI CTAG 3 cut(s) 221, 498, 657
GlaI GCGC 2 cut(s) 926, 1629
GluI GCNGC 5 cut(s) 243, 557, 911, 1317, 1593
GsuI CTGGAG 3 cut(s) 955, 1173, 1332
HaeII RGCGCY 2 cut(s) 928, 1631
HaeIII GGCC 3 cut(s) 319, 1378, 1751
HapII CCGG 3 cut(s) 369, 728, 1414
HgaI GACGC 1 cut(s) 1710
HhaI GCGC 2 cut(s) 927, 1630
Hin1I GRCGYC 1 cut(s) 925
Hin1II CATG 4 cut(s) 580, 1010, 1547, 1709
Hin6I GCGC 2 cut(s) 925, 1628
HinP1I GCGC 2 cut(s) 925, 1628
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 8 cut(s) 104, 112, 485, 1001, 1307, 1556, 1655, 1807
HpaII CCGG 3 cut(s) 369, 728, 1414
HphI GGTGA 8 cut(s) 155, 325, 341, 972, 1040, 1316, 1384, 1854
Hpy166II GTNNAC 1 cut(s) 749
Hpy8I GTNNAC 1 cut(s) 749
HpyCH4IV ACGT 2 cut(s) 1174, 1230
HpyCH4V TGCA 6 cut(s) 242, 460, 529, 709, 1010, 1595
HpyF10VI GCNNNNNNNGC 2 cut(s) 1244, 1601
HpyF3I CTNAG 5 cut(s) 227, 426, 560, 781, 1637
HpySE526I ACGT 2 cut(s) 1174, 1230
Hsp92I GRCGYC 1 cut(s) 925
Hsp92II CATG 4 cut(s) 580, 1010, 1547, 1709
HspAI GCGC 2 cut(s) 925, 1628
KasI GGCGCC 1 cut(s) 924
KflI GGGWCCC 1 cut(s) 661
Ksp22I TGATCA 2 cut(s) 331, 400
KspI CCGCGG 1 cut(s) 1400
LmnI GCTCC 5 cut(s) 44, 349, 1192, 1210, 1313
Lsp1109I GCAGC 5 cut(s) 254, 543, 897, 1328, 1579
LweI GCATC 4 cut(s) 965, 1176, 1579, 1759
MaeI CTAG 3 cut(s) 221, 498, 657
MaeII ACGT 2 cut(s) 1174, 1230
MaeIII GTNAC 4 cut(s) 23, 490, 947, 1849
MflI RGATCY 2 cut(s) 633, 1427
MhlI GDGCHC 3 cut(s) 1189, 1606, 1705
MluCI AATT 5 cut(s) 34, 394, 410, 619, 1671
Mly113I GGCGCC 1 cut(s) 925
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 4 cut(s) 371, 820, 842, 1232
Mph1103I ATGCAT 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 1363
MseI TTAA 1 cut(s) 863
MspA1I CMGCKG 2 cut(s) 1399, 1766
MspI CCGG 3 cut(s) 369, 728, 1414
MspR9I CCNGG 4 cut(s) 370, 1302, 1414, 1515
Mva1269I GAATGC 1 cut(s) 1620
MvaI CCWGG 2 cut(s) 1302, 1515
MvnI CGCG 1 cut(s) 1399
MwoI GCNNNNNNNGC 2 cut(s) 1244, 1601
NarI GGCGCC 1 cut(s) 925
NciI CCSGG 2 cut(s) 370, 1414
NdeI CATATG 1 cut(s) 892
NlaIII CATG 4 cut(s) 580, 1010, 1547, 1709
NlaIV GGNNCC 7 cut(s) 662, 663, 872, 926, 1239, 1299, 1603
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 1 cut(s) 1010
PaeR7I CTCGAG 2 cut(s) 187, 201
PagI TCATGA 1 cut(s) 1705
PctI GAATGC 1 cut(s) 1620
PdmI GAANNNNTTC 1 cut(s) 1363
PfeI GAWTC 7 cut(s) 104, 485, 1001, 1307, 1556, 1655, 1807
PflMI CCANNNNNTGG 1 cut(s) 326
PfoI TCCNGGA 2 cut(s) 368, 1412
PinAI ACCGGT 1 cut(s) 727
PkrI GCNGC 5 cut(s) 244, 558, 912, 1318, 1594
Ple19I CGATCG 1 cut(s) 946
PleI GAGTC 1 cut(s) 106
PluTI GGCGCC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 1175
PpuMI RGGWCCY 2 cut(s) 661, 1298
PshAI GACNNNNGTC 1 cut(s) 1516
PsiI TTATAA 2 cut(s) 600, 827
Psp1406I AACGTT 1 cut(s) 1230
Psp5II RGGWCCY 2 cut(s) 661, 1298
Psp6I CCWGG 2 cut(s) 1300, 1513
PspGI CCWGG 2 cut(s) 1300, 1513
PspN4I GGNNCC 7 cut(s) 662, 663, 872, 926, 1239, 1299, 1603
PspPI GGNCC 2 cut(s) 661, 1298
PspPPI RGGWCCY 2 cut(s) 661, 1298
PspXI VCTCGAGB 1 cut(s) 187
PstI CTGCAG 2 cut(s) 244, 1597
PsuI RGATCY 2 cut(s) 633, 1427
PvuI CGATCG 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 1766
RsaI GTAC 1 cut(s) 1177
RsaNI GTAC 1 cut(s) 1176
SacII CCGCGG 1 cut(s) 1400
SaqAI TTAA 1 cut(s) 863
SatI GCNGC 5 cut(s) 243, 557, 911, 1317, 1593
Sau96I GGNCC 2 cut(s) 661, 1298
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 4 cut(s) 370, 1302, 1414, 1515
SduI GDGCHC 3 cut(s) 1189, 1606, 1705
SfaNI GCATC 4 cut(s) 965, 1176, 1579, 1759
SfcI CTRYAG 3 cut(s) 240, 669, 1593
SfoI GGCGCC 1 cut(s) 926
Sfr274I CTCGAG 2 cut(s) 187, 201
Sfr303I CCGCGG 1 cut(s) 1400
SgrBI CCGCGG 1 cut(s) 1400
SinI GGWCC 2 cut(s) 661, 1298
SlaI CTCGAG 2 cut(s) 187, 201
SmlI CTYRAG 3 cut(s) 187, 201, 281
SmoI CTYRAG 3 cut(s) 187, 201, 281
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 5 cut(s) 34, 394, 410, 619, 1671
SsiI CCGC 5 cut(s) 268, 1397, 1399, 1563, 1695
SspDI GGCGCC 1 cut(s) 924
SspMI CTAG 3 cut(s) 221, 498, 657
StyD4I CCNGG 4 cut(s) 368, 1300, 1412, 1513
StyI CCWWGG 2 cut(s) 656, 849
TaiI ACGT 2 cut(s) 1177, 1233
TaqI TCGA 8 cut(s) 102, 188, 202, 258, 942, 1262, 1554, 1679
TasI AATT 5 cut(s) 34, 394, 410, 619, 1671
TfiI GAWTC 7 cut(s) 104, 485, 1001, 1307, 1556, 1655, 1807
Tru1I TTAA 1 cut(s) 863
Tru9I TTAA 1 cut(s) 863
TscAI CASTG 1 cut(s) 1072
TseI GCWGC 5 cut(s) 242, 556, 910, 1316, 1592
TspDTI ATGAA 4 cut(s) 423, 879, 1560, 1817
TspRI CASTG 1 cut(s) 1072
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 2 cut(s) 661, 1298
XapI RAATTY 2 cut(s) 394, 1671
XceI RCATGY 1 cut(s) 1010
XhoI CTCGAG 2 cut(s) 187, 201
XmaJI CCTAGG 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 748
XmnI GAANNNNTTC 1 cut(s) 1363
XspI CTAG 3 cut(s) 221, 498, 657
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.