AT3G20395

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011136
Physical Location & Seq
Reverse (-)
7111934 .. 7113899
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20395.1

Sequence Viewer

Length: 672 bp
ATGGGCATTTCTAAGATGATTCTTCTGAAATTCACATATGTTGTTTCTTTCTGGATTACTTGCGTGATCTTCAAAATGTCAAGAACGATAAAACGAGCTTTGTTGGGCTCATTCATCTTCATTCTTGCTTCCGCTAGTGTTGTGGTAGCCGCCATTGTAGGAGCCATCGAAGGTCACACCACTGACATCGGATTTCTTCAAGGTAGTGTGCTTGGAGTGGTGGCTGGAGTTATCACTGCGGTCCAACTCTTTGGACCGGTGCTACATAGTGATCAACCTTTGTCTAAGGTGGCTTTACTCAGGAGAGTAGTGAATGGGAAAGCCATTATGGGATTGGTTAGACCTTTTGTTCTCAAAGCATATCAATGGCAAATAATAGCATTGGATACAAGTTACATGGAGAGTTCAAACTTATACGATTTCAATCATGAAAAAAAGGGACTATCGAAGAGCTCTATTCAGAACATCCCGATGTTCTACAACCGTTCAGAACATCAAACAAAATCGAGTTGCTCGATTTGCTTACAGGATTGGGAAGAAGGGGAAGTAGGAAGAAAGCTAGCAAGATGTGGCCACACATTTCATATGAATTGCATAGATGAGTGGTTGCTTAGACAAGAAACTTGCCCCATTTGTAGAGACCATCTTTCACATAACACAACTTCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

223

Amino Acids

24.89

Weight (kDa)

8.99

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 167 - 213 1.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 170 - 213 1.1e-13 Ring finger domain
zf-RING_11 PF17123 171 - 199 9.2e-09 RING-like zinc finger
zf-C3HC4 PF00097 171 - 212 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 171 - 204 1.4e-06 RING-type zinc-finger
zf-C3HC4_2 PF13923 171 - 212 6.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 171 - 213 4.8e-07 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 132, 150, 239
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 1 cut(s) 29
AgeI ACCGGT 1 cut(s) 256
AgsI TTSAA 4 cut(s) 73, 200, 408, 424
AluBI AGCT 3 cut(s) 98, 453, 559
AluI AGCT 3 cut(s) 98, 453, 559
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 1 cut(s) 633
AoxI GGCC 1 cut(s) 571
ApoI RAATTY 1 cut(s) 29
AsiGI ACCGGT 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 241, 254
AsuNHI GCTAGC 1 cut(s) 559
AvaII GGWCC 2 cut(s) 241, 254
BalI TGGCCA 1 cut(s) 573
BanII GRGCYC 2 cut(s) 110, 455
Bbv12I GWGCWC 1 cut(s) 455
BccI CCATC 2 cut(s) 173, 651
BciVI GTATCC 1 cut(s) 379
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 2 cut(s) 135, 560
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme18I GGWCC 2 cut(s) 241, 254
BmgT120I GGNCC 2 cut(s) 241, 254
BmiI GGNNCC 1 cut(s) 163
BmtI GCTAGC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 633
BsaWI WCCGGW 1 cut(s) 256
Bse118I RCCGGY 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 423
BseGI GGATG 1 cut(s) 465
BseJI GATNNNNATC 1 cut(s) 423
BseMII CTCAG 1 cut(s) 313
BshFI GGCC 1 cut(s) 573
BshTI ACCGGT 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 455
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 1 cut(s) 573
Bso31I GGTCTC 1 cut(s) 633
Bsp1286I GDGCHC 2 cut(s) 110, 455
Bsp143I GATC 2 cut(s) 66, 271
BspACI CCGC 3 cut(s) 132, 150, 239
BspANI GGCC 1 cut(s) 573
BspCNI CTCAG 1 cut(s) 312
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 163
BspOI GCTAGC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 256
BssMI GATC 2 cut(s) 66, 271
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 4 cut(s) 12, 285, 299, 611
BstF5I GGATG 1 cut(s) 465
BstKTI GATC 2 cut(s) 69, 274
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 2 cut(s) 66, 271
BstMWI GCNNNNNNNGC 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 251
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 573
BtsCI GGATG 1 cut(s) 465
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 2 cut(s) 180, 234
Cac8I GCNNGC 1 cut(s) 561
CciI TCATGA 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 241, 254
CspAI ACCGGT 1 cut(s) 256
CviAII CATG 2 cut(s) 397, 428
DdeI CTNAG 4 cut(s) 12, 285, 299, 611
DpnI GATC 2 cut(s) 68, 273
DpnII GATC 2 cut(s) 66, 271
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 110, 455
Eco31I GGTCTC 1 cut(s) 633
Eco47I GGWCC 2 cut(s) 241, 254
Eco53kI GAGCTC 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 453
EcoT38I GRGCYC 2 cut(s) 110, 455
FaeI CATG 2 cut(s) 400, 431
FaqI GGGAC 1 cut(s) 453
FatI CATG 2 cut(s) 396, 427
FauNDI CATATG 2 cut(s) 37, 585
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 2 cut(s) 110, 455
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 2 cut(s) 135, 560
GluI GCNGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 573
HapII CCGG 1 cut(s) 257
Hin1II CATG 2 cut(s) 400, 431
HinfI GANTC 1 cut(s) 19
HpaII CCGG 1 cut(s) 257
Hpy188I TCNGA 4 cut(s) 27, 191, 462, 490
Hpy188III TCNNGA 5 cut(s) 52, 81, 301, 428, 469
HpyAV CCTTC 2 cut(s) 164, 533
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 594
HpyF10VI GCNNNNNNNGC 1 cut(s) 519
HpyF3I CTNAG 4 cut(s) 12, 285, 299, 611
Hsp92II CATG 2 cut(s) 400, 431
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 2 cut(s) 66, 271
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 5 cut(s) 37, 210, 270, 286, 512
MaeI CTAG 2 cut(s) 135, 560
MaeIII GTNAC 2 cut(s) 173, 392
MalI GATC 2 cut(s) 68, 273
MboI GATC 2 cut(s) 66, 271
MboII GAAGA 7 cut(s) 14, 61, 109, 188, 460, 548, 564
MhlI GDGCHC 2 cut(s) 110, 455
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 2 cut(s) 29, 589
MluNI TGGCCA 1 cut(s) 573
MmeI TCCRAC 1 cut(s) 268
Mox20I TGGCCA 1 cut(s) 573
MscI TGGCCA 1 cut(s) 573
MslI CAYNNNNRTG 2 cut(s) 364, 470
Msp20I TGGCCA 1 cut(s) 573
MspI CCGG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 519
NdeI CATATG 2 cut(s) 37, 585
NdeII GATC 2 cut(s) 66, 271
NheI GCTAGC 1 cut(s) 559
NlaIII CATG 2 cut(s) 400, 431
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 173
PagI TCATGA 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PfeI GAWTC 1 cut(s) 19
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 455
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 2 cut(s) 241, 254
RseI CAYNNNNRTG 2 cut(s) 364, 470
SacI GAGCTC 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 443
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 66, 271
Sau96I GGNCC 2 cut(s) 241, 254
SduI GDGCHC 2 cut(s) 110, 455
SetI ASST 8 cut(s) 100, 175, 205, 280, 291, 346, 455, 561
SinI GGWCC 2 cut(s) 241, 254
SmiMI CAYNNNNRTG 2 cut(s) 364, 470
Sse9I AATT 2 cut(s) 29, 589
SsiI CCGC 3 cut(s) 132, 150, 239
SspMI CTAG 2 cut(s) 135, 560
SstI GAGCTC 1 cut(s) 455
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 4 cut(s) 168, 446, 506, 515
TasI AATT 2 cut(s) 29, 589
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 1 cut(s) 19
TscAI CASTG 2 cut(s) 187, 241
TseFI GTSAC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 5 cut(s) 103, 109, 444, 572, 602
TspRI CASTG 2 cut(s) 187, 241
VpaK11BI GGWCC 2 cut(s) 241, 254
XapI RAATTY 1 cut(s) 29
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XspI CTAG 2 cut(s) 135, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.