RLG00000033930

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011136
Physical Location & Seq
Reverse (-)
34316035 .. 34317523
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033930

Sequence Viewer

Length: 711 bp
ATGTCTAGTTTGTGCAAGGAATTGTTTTCAGGCTTATTGGAGGGTGCATTGAGATCTAAAGAGGTTGTTTCTTTGTGGGTTTTGGCTACACTAGAAGGTTTGGGAATTGGAGCTTTGAATGGAGTCACTAAGAGAGTTCTTCTTTCTGCATTTACCTGCATTCTTGCAATAGGAGGAGCTATAGCGGGAGCAATTCATGGAGCCATCAAAGGCCAGACGACAGAAACTGGGTTTTCTAGTGGAGCCGGAATAGGTGGTTTGGCAGGGGCTATTGCTGCCATTCAATTGATGGATTTGATAGTTGATGGGCATCCATTGTCTAAGGTTGCTCTCTTAGGTGGGATGGTGAATGGGAAGGTGTTTACGGAGTGGATGAGCTCTGCATTGCTAAAAGCCTATCATTGGCAAGTTAGCACGCTGGAGACTTACAGAGAGATTTCTGAACTCTATGACATCACTAGAGTTGGGGGATTATCCCAAGAATGCATTCAAAATCTCCCTCAAAGTATGTTTTGTTCTAGCAATATCATTGAGTCTTCCGATGAGTTTTGCTGCTCCATTTGCTTACAGGAATTAGAGGATGGAGAGTATACAAGAGAACTTCCCATCTGTAGGCACTTGTTTCACTTGGCCTGCATAGACCTATGGCTAAAGCGACAAGGCTCTTGCCCTATTTGTAGAGTCCATGTCAGTGACAGCGCAAGATTATGA

Protein Analysis

237

Amino Acids

25.3

Weight (kDa)

5.51

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 183 - 227 1.1e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 184 - 227 7.7e-13 Ring finger domain
zf-RING_11 PF17123 185 - 213 1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 185 - 226 7.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 185 - 224 4.6e-06 RING-type zinc-finger
zf-C3HC4 PF00097 185 - 226 5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 590
AciI CCGC 1 cut(s) 185
AfiI CCNNNNNNNGG 2 cut(s) 402, 612
AgsI TTSAA 3 cut(s) 118, 284, 491
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 3 cut(s) 113, 179, 378
AluI AGCT 3 cut(s) 113, 179, 378
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 416
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 2 cut(s) 211, 630
ApeKI GCWGC 2 cut(s) 275, 552
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 701
AsuHPI GGTGA 1 cut(s) 358
BanII GRGCYC 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 2 cut(s) 262, 539
BccI CCATC 6 cut(s) 212, 283, 299, 337, 575, 614
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 5 cut(s) 6, 92, 237, 459, 519
BfmI CTRYAG 2 cut(s) 180, 610
BfuAI ACCTGC 1 cut(s) 164
BglII AGATCT 1 cut(s) 53
BisI GCNGC 2 cut(s) 276, 553
BlsI GCNGC 2 cut(s) 277, 554
BmiI GGNNCC 2 cut(s) 202, 244
BmrI ACTGGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 237
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 440
BsaBI GATNNNNATC 1 cut(s) 309
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 612
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 309
BseGI GGATG 4 cut(s) 310, 348, 378, 586
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 402, 612
BseMI GCAATG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 1 cut(s) 189
BseXI GCAGC 2 cut(s) 262, 539
BshFI GGCC 2 cut(s) 213, 632
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 402, 612
BsmAI GTCTC 1 cut(s) 416
BsmI GAATGC 3 cut(s) 159, 486, 488
BsnI GGCC 2 cut(s) 213, 632
Bsp1286I GDGCHC 1 cut(s) 380
Bsp143I GATC 1 cut(s) 53
BspACI CCGC 1 cut(s) 185
BspANI GGCC 2 cut(s) 213, 632
BspLI GGNNCC 2 cut(s) 202, 244
BspMI ACCTGC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 383
BsrI ACTGG 1 cut(s) 232
BssMI GATC 1 cut(s) 53
BssNAI GTATAC 1 cut(s) 591
Bst1107I GTATAC 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 416, 634
BstDEI CTNAG 3 cut(s) 129, 321, 334
BstF5I GGATG 4 cut(s) 310, 348, 378, 586
BstHHI GCGC 1 cut(s) 701
BstKTI GATC 1 cut(s) 56
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 1 cut(s) 53
BstMWI GCNNNNNNNGC 2 cut(s) 275, 561
BstSFI CTRYAG 2 cut(s) 180, 610
BstV1I GCAGC 2 cut(s) 262, 539
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BstZ17I GTATAC 1 cut(s) 591
BsuRI GGCC 2 cut(s) 213, 632
BtsCI GGATG 4 cut(s) 310, 348, 378, 586
BtsIMutI CAGTG 1 cut(s) 697
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 2 cut(s) 416, 634
CaiI CAGNNNCTG 1 cut(s) 227
CfoI GCGC 1 cut(s) 701
CviAII CATG 2 cut(s) 197, 686
DdeI CTNAG 3 cut(s) 129, 321, 334
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 1 cut(s) 380
FaeI CATG 2 cut(s) 200, 689
FaiI YATR 9 cut(s) 182, 198, 450, 509, 591, 638, 646, 687, 709
FatI CATG 2 cut(s) 196, 685
FauI CCCGC 1 cut(s) 178
FblI GTMKAC 1 cut(s) 590
Fnu4HI GCNGC 2 cut(s) 276, 553
FokI GGATG 4 cut(s) 297, 355, 385, 593
FriOI GRGCYC 1 cut(s) 380
Fsp4HI GCNGC 2 cut(s) 276, 553
FspBI CTAG 5 cut(s) 6, 92, 237, 459, 519
GlaI GCGC 1 cut(s) 700
GluI GCNGC 2 cut(s) 276, 553
GsuI CTGGAG 1 cut(s) 440
HaeIII GGCC 2 cut(s) 213, 632
HapII CCGG 1 cut(s) 246
HhaI GCGC 1 cut(s) 701
Hin1II CATG 2 cut(s) 200, 689
Hin6I GCGC 1 cut(s) 699
HinP1I GCGC 1 cut(s) 699
HinfI GANTC 3 cut(s) 123, 533, 681
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 2 cut(s) 363, 591
Hpy188I TCNGA 2 cut(s) 442, 541
Hpy8I GTNNAC 2 cut(s) 363, 591
HpyAV CCTTC 2 cut(s) 89, 349
HpyCH4V TGCA 8 cut(s) 15, 47, 149, 159, 167, 383, 486, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 561
HpyF3I CTNAG 3 cut(s) 129, 321, 334
Hsp92II CATG 2 cut(s) 200, 689
HspAI GCGC 1 cut(s) 699
Kzo9I GATC 1 cut(s) 53
LmnI GCTCC 6 cut(s) 110, 176, 188, 200, 242, 560
LpnPI CCDG 9 cut(s) 15, 169, 213, 227, 249, 259, 404, 554, 646
Lsp1109I GCAGC 2 cut(s) 262, 539
LweI GCATC 1 cut(s) 319
MaeI CTAG 5 cut(s) 6, 92, 237, 459, 519
MaeIII GTNAC 2 cut(s) 124, 692
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 2 cut(s) 131, 528
MfeI CAATTG 1 cut(s) 284
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 380
MluCI AATT 5 cut(s) 20, 105, 192, 284, 572
MlyI GAGTC 3 cut(s) 132, 542, 690
MnlI CCTC 5 cut(s) 34, 55, 167, 510, 571
Mph1103I ATGCAT 1 cut(s) 488
MroXI GAANNNNTTC 1 cut(s) 486
MslI CAYNNNNRTG 1 cut(s) 690
MspI CCGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 284
Mva1269I GAATGC 3 cut(s) 159, 486, 488
MwoI GCNNNNNNNGC 2 cut(s) 275, 561
NdeII GATC 1 cut(s) 53
NlaIII CATG 2 cut(s) 200, 689
NlaIV GGNNCC 2 cut(s) 202, 244
NmuCI GTSAC 2 cut(s) 124, 692
NsiI ATGCAT 1 cut(s) 488
PctI GAATGC 3 cut(s) 159, 486, 488
PdmI GAANNNNTTC 1 cut(s) 486
PkrI GCNGC 2 cut(s) 277, 554
PleI GAGTC 3 cut(s) 131, 541, 689
PpsI GAGTC 3 cut(s) 131, 541, 689
Psp124BI GAGCTC 1 cut(s) 380
PspN4I GGNNCC 2 cut(s) 202, 244
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 690
SacI GAGCTC 1 cut(s) 380
SatI GCNGC 2 cut(s) 276, 553
Sau3AI GATC 1 cut(s) 53
SchI GAGTC 3 cut(s) 132, 542, 690
SduI GDGCHC 1 cut(s) 380
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 2 cut(s) 180, 610
SmiMI CAYNNNNRTG 1 cut(s) 690
Sse9I AATT 5 cut(s) 20, 105, 192, 284, 572
SsiI CCGC 1 cut(s) 185
SspMI CTAG 5 cut(s) 6, 92, 237, 459, 519
SstI GAGCTC 1 cut(s) 380
TasI AATT 5 cut(s) 20, 105, 192, 284, 572
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 2 cut(s) 124, 692
TseI GCWGC 2 cut(s) 275, 552
Tsp45I GTSAC 2 cut(s) 124, 692
TspDTI ATGAA 1 cut(s) 185
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 590
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 5 cut(s) 6, 92, 237, 459, 519
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.