Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002530

Physical Location & Seq

Genomic Coordinates Forward (+)

7143418 .. 7151047

Gene Structure 7630 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G20475.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGGAGGAAATGGAAGACACTGAAACCGAACCACAGGTTTACATGGCTTGTATTCAGCACGGTCGGAGAGTTGGAGTTTCTTACTACGACTGTAGTGTACGCCAGCTTCATGTGCTAGAATTTTGGGAAGAAGATTGCTCAGATTTTACATTGATCAATATGGTAAAATATCAAGCGAAGCCATCGATCATTTACGCAAGCACGAAAAGTGAAGAATCCTTTGTAGCTGCTTTGCAGCAGAATGACGGAACTGACGAGACTACCATGGTAAAGCTGGTAAAGAGCTCAACATTCAGCTACGAGCAAGCGTGGCACAGACTGGTATATCTTCGAGTAACTGGAATGGATGATGGATTGAACATCAAAGAAAGGATTTGTTATCTGAGTTCCATGATGGATGTGGGCAGTGAAGTCCAAGTTCGTGTTAGTGGTGGTCTTCTTGCTATATTAGAAAGCGAACGAATTGTAGAAACCCTGGAACAAAACGAATCTGGGAGTGCATCAATCGCAATTGATTCAGTCATGGAAGTACCATTGAACAAGTTTCTTAAACTTGATGCTGCTGCTCACGAGGCTCTGCAGATATTTCAGACAGATAAACATCCAAGCCATATGGGCATTGGCCGGGCCAAAGAAGGGTTCTCGGTATTTGGAATGATGAATAAGTGTGCCACGCCAATGGGTAGACGCCTTTTAAGAAGCTGGTTTATGAGACCAATTTTAGATCTTGAAGTGTTAGATCGCCGTCTCAATGCTATTTCCTTTTTCATTTCTTCAGTAGAGCTGATGGCATCATTGCGGGAGACACTGAAATCAGTGAAGGACATTTCACATCTACTCAAGAAATTCAACTCTCCGACGTCCCTCTGTACCAGTAACGACTGGACAGCTTTCTTGAAGAGCATAAGTGCGCTCCTGCACGTGAATAAGATATTTGAAGTTGGAGTTTCAGAAAGTCTCAGAGAGCATATGAGACGCTTCAACTTGGACATTATTGAGAAGGCCGGCTTATGTATCAGCACAGAGCTAGATTATGTCTATGAACTGGTCATTGGAGTCATTGATGTTACTAGAAGCAAAGAGAGGGGATATCAAACTTTGGTTAAAGAAGGATTCTGTGCTGAGTTGGATGAGCTCAGGCAAATATATGAGGAGTTGCCAGAATTTCTGCAGGAGGTTTCAGCGATGGAGTTAGAACACTTTCCTCATTTGCATAAGGAAAAGCTTCCTCCTTGTATCGTCTATATTCAACAAATTGGGTACCTCATGTGTATCTTTGGAGAAAAGCTTGATGAAACTGCTCTTAATAGGCTTACTGAATTTGAATTTGCGTTTTCTGATATGGATGGAGAGACTCAGCGATTCTTTTACCATACCTCGAAGACACGAGAGTTAGACAACCTTCTTGGAGATATCTACCACAAAATTTTAGATATGGAAAGGGCAATTATTAGGGACTTGCTGTCACACACACTTTTGTTCTCGGCTCACCTGCTGAAGGCAGTTAACTTTGTTGCAGAACTTGATTGCATTTTATCGTTGGCTTGTGTAGCCCATCAGAATAACTACGTAAGGCCTGTCCTGACAGTAGAATCATTGCTTGATATTCGAAATGGAAGGCATGTTTTGCAGGAAATGGCTGTAGATACTTTTATCCCAAACGACACTGAAATCAATGATAATGGACGAATTCATATAATTACCGGGCCTAATTACTCAGGAAAGAGCATATATGTAAAGCAGGTGGCGTTAATTGTTTTCCTATCCCATATTGGAAGCTTTGTACCAGCAGATGCAGCAACTGTTGGTTTAACTGACAGGATCTTTTGTGCAATGGGAAGCAAGTTCATGACCGCGGAGCAATCTACATTCATGATAGATCTGCATCAAGTAGGAATGATGCTCAGGCAGGCAACTTCAAGATCTCTGTGTCTCTTAGACGAGTTTGGTAAAGGCACTCTTACAGAAGATGGTATTGGCTTGCTTGGTGGGACAATTAGTCACTTTGCTACATGTGCTGAGCCACCAAGGGTTGTAGTATGTACGCACTTGACTGAGCTACTTAACGAGAGCTGCTTGCCTGTTTCTGAGAAGATTAAGTTCTACACAATGAGCGTTCTTAGGCCAGACACAGAATCTGCAAACATGGAAGAGATTGTTTTTCTTTATAGGTTAATTCCGGGACAAACTTTGCTGAGCTATGGCCTTCACTGTGCGCTACTCGCTGGTGTCCCGGAGGAAGTCGTGAAGAGAGCAGCCATCGTGTTGGACGCCTTTGAGAGTAACAACAACGTCGATAAACTAAGCCTTGACAAAATATCGTCTCAAGATCAAGCATTCAAGGATGCTGTTGACAAGTTTGCGGAGCTTGACATCAGTAAAGGTGACATCCATGCCTTCTTTCAAGATATCTTCACTTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000710 GO:0000712 GO:0000793 GO:0000794 GO:0000795 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0010777 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042623 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0098813 GO:0099086 GO:0140013 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MutS_III MutS_IV MutS_V

Protein Analysis

807

Amino Acids

91.12

Weight (kDa)

5.11

Isoelectric Point (pI)

38.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_III	PF05192	189 - 515	1.8e-32	MutS domain III
MutS_V	PF00488	566 - 737	8.7e-38	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20475 AT3G20475 AT3G20475
fragaria_vesca	FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000
malus_domestica	MD11G1214700.v1.1
prunus_persica	Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0040391 RchiOBHm_Chr5g0040451
rosa_laevigata	RLG00000033976
rosa_multiflora	Rmu_sc0004516.1_g000007
rosa_roxburghii	Rroxscaffold_1G00040210 Rroxscaffold_1G00040250 Rroxscaffold_1G00040300 Rroxscaffold_1G00040940
rosa_rugosa	Rorug05G0184700
rosa_samantha	Rh5AG272000 Rh5BG275900 Rh5CG308000 Rh5DG284100
rosa_wichuraiana	Rw5G025430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1500, 1732
AatII	GACGTC	1 cut(s)	863
Acc36I	ACCTGC	2 cut(s)	1500, 1732
Acc65I	GGTACC	1 cut(s)	1260
AccB1I	GGYRCC	1 cut(s)	1260
AccI	GTMKAC	1 cut(s)	685
AccII	CGCG	1 cut(s)	1856
AciI	CCGC	4 cut(s)	799, 1854, 1856, 2363
AclWI	GGATC	1 cut(s)	1829
AcoI	YGGCCR	1 cut(s)	622
AcsI	RAATTY	7 cut(s)	119, 845, 1163, 1319, 1325, 1425, 1689
AcuI	CTGAAG	2 cut(s)	759, 1517
AcvI	CACGTG	1 cut(s)	922
AcyI	GRCGYC	3 cut(s)	688, 860, 2271
AfaI	GTAC	6 cut(s)	99, 531, 871, 1262, 1785, 2044
AfiI	CCNNNNNNNGG	2 cut(s)	636, 1498
AflIII	ACRYGT	1 cut(s)	2012
AhdI	GACNNNNNGTC	2 cut(s)	1462, 1998
AjnI	CCWGG	1 cut(s)	474
AjuI	GAANNNNNNNTTGG	6 cut(s)	623, 655, 1035, 1067, 1959, 1991
Alw21I	GWGCWC	2 cut(s)	287, 1137
Alw26I	GTCTC	9 cut(s)	251, 706, 752, 797, 962, 967, 1346, 1937, 2328
AlwI	GGATC	1 cut(s)	1829
AlwNI	CAGNNNCTG	2 cut(s)	1802, 2138
AoxI	GGCC	7 cut(s)	622, 627, 1002, 1574, 1706, 2123, 2203
ApeKI	GCWGC	7 cut(s)	227, 235, 560, 563, 1796, 2073, 2255
ApoI	RAATTY	7 cut(s)	119, 845, 1163, 1319, 1325, 1425, 1689
Asp700I	GAANNNNTTC	2 cut(s)	1200, 1224
Asp718I	GGTACC	1 cut(s)	1260
AspLEI	GCGC	2 cut(s)	913, 2218
AspS9I	GGNCC	2 cut(s)	627, 1706
AsuC2I	CCSGG	4 cut(s)	626, 1705, 2181, 2234
AsuHPI	GGTGA	2 cut(s)	1481, 2396
AsuII	TTCGAA	1 cut(s)	1609
BanI	GGYRCC	1 cut(s)	1260
BanII	GRGCYC	2 cut(s)	287, 1137
BarI	GAAGNNNNNNTAC	2 cut(s)	90, 122
BauI	CACGAG	2 cut(s)	569, 1386
BbrPI	CACGTG	1 cut(s)	922
BbsI	GAAGAC	3 cut(s)	21, 428, 1388
Bbv12I	GWGCWC	2 cut(s)	287, 1137
BbvI	GCAGC	7 cut(s)	214, 247, 547, 550, 1808, 2060, 2267
BccI	CCATC	9 cut(s)	190, 344, 388, 781, 1180, 1340, 1563, 1964, 2267
BceAI	ACGGC	1 cut(s)	729
BciT130I	CCWGG	1 cut(s)	476
BclI	TGATCA	1 cut(s)	153
BcnI	CCSGG	4 cut(s)	626, 1705, 2181, 2234
BcoDI	GTCTC	9 cut(s)	251, 706, 752, 797, 962, 967, 1346, 1937, 2328
BfaI	CTAG	3 cut(s)	116, 1028, 1071
BfmI	CTRYAG	4 cut(s)	91, 578, 1169, 1641
BfuAI	ACCTGC	2 cut(s)	1500, 1732
BglI	GCCNNNNNGGC	1 cut(s)	615
BglII	AGATCT	3 cut(s)	724, 1879, 1922
BisI	GCNGC	7 cut(s)	228, 236, 561, 564, 1797, 2074, 2256
BlpI	GCTNAGC	2 cut(s)	2019, 2195
BlsI	GCNGC	7 cut(s)	229, 237, 562, 565, 1798, 2075, 2257
Bme1390I	CCNGG	5 cut(s)	476, 626, 1705, 2181, 2234
BmeRI	GACNNNNNGTC	2 cut(s)	1462, 1998
BmgT120I	GGNCC	2 cut(s)	627, 1706
BmiI	GGNNCC	1 cut(s)	1262
BmrFI	CCNGG	5 cut(s)	476, 626, 1705, 2181, 2234
BmsI	GCATC	7 cut(s)	509, 547, 800, 1783, 1890, 1894, 2335
BpiI	GAAGAC	3 cut(s)	21, 428, 1388
Bpu10I	CCTNAGC	2 cut(s)	1136, 1904
Bpu1102I	GCTNAGC	2 cut(s)	2019, 2195
Bpu14I	TTCGAA	1 cut(s)	1609
BpuEI	CTTGAG	2 cut(s)	824, 2310
BpuMI	CCSGG	4 cut(s)	626, 1705, 2181, 2234
Bsa29I	ATCGAT	1 cut(s)	185
BsaAI	YACGTR	2 cut(s)	922, 1570
BsaBI	GATNNNNATC	2 cut(s)	600, 1884
BsaHI	GRCGYC	3 cut(s)	688, 860, 2271
BsaI	GGTCTC	1 cut(s)	706
BsaJI	CCNNGG	4 cut(s)	264, 474, 1854, 2027
Bsc4I	CCNNNNNNNGG	2 cut(s)	636, 1498
Bse118I	RCCGGY	1 cut(s)	1004
Bse1I	ACTGG	5 cut(s)	324, 343, 873, 887, 1050
Bse3DI	GCAATG	3 cut(s)	794, 1595, 1839
Bse8I	GATNNNNATC	2 cut(s)	600, 1884
BseBI	CCWGG	1 cut(s)	476
BseCI	ATCGAT	1 cut(s)	185
BseDI	CCNNGG	4 cut(s)	264, 474, 1854, 2027
BseGI	GGATG	7 cut(s)	352, 403, 601, 1135, 1351, 2350, 2388
BseJI	GATNNNNATC	2 cut(s)	600, 1884
BseLI	CCNNNNNNNGG	2 cut(s)	636, 1498
BseMI	GCAATG	3 cut(s)	794, 1595, 1839
BseNI	ACTGG	5 cut(s)	324, 343, 873, 887, 1050
BseRI	GAGGAG	1 cut(s)	1166
BseXI	GCAGC	7 cut(s)	214, 247, 547, 550, 1808, 2060, 2267
BsgI	GTGCAG	1 cut(s)	902
Bsh1236I	CGCG	1 cut(s)	1856
Bsh1285I	CGRYCG	1 cut(s)	64
BshFI	GGCC	7 cut(s)	624, 629, 1004, 1576, 1708, 2125, 2205
BshNI	GGYRCC	1 cut(s)	1260
BshVI	ATCGAT	1 cut(s)	185
BsiEI	CGRYCG	1 cut(s)	64
BsiHKAI	GWGCWC	2 cut(s)	287, 1137
BsiSI	CCGG	5 cut(s)	625, 1005, 1704, 2180, 2234
BslFI	GGGAC	5 cut(s)	847, 1469, 2005, 2196, 2216
BslI	CCNNNNNNNGG	2 cut(s)	636, 1498
BsmAI	GTCTC	9 cut(s)	251, 706, 752, 797, 962, 967, 1346, 1937, 2328
BsmBI	CGTCTC	3 cut(s)	752, 967, 2328
BsmFI	GGGAC	5 cut(s)	847, 1469, 2005, 2196, 2216
BsmI	GAATGC	1 cut(s)	2336
BsnI	GGCC	7 cut(s)	624, 629, 1004, 1576, 1708, 2125, 2205
Bso31I	GGTCTC	1 cut(s)	706
Bsp119I	TTCGAA	1 cut(s)	1609
Bsp1286I	GDGCHC	2 cut(s)	287, 1137
Bsp143I	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
Bsp1720I	GCTNAGC	2 cut(s)	2019, 2195
Bsp19I	CCATGG	1 cut(s)	264
BspACI	CCGC	4 cut(s)	799, 1854, 1856, 2363
BspANI	GGCC	7 cut(s)	624, 629, 1004, 1576, 1708, 2125, 2205
BspDI	ATCGAT	1 cut(s)	185
BspFNI	CGCG	1 cut(s)	1856
BspHI	TCATGA	2 cut(s)	1848, 1872
BspLI	GGNNCC	1 cut(s)	1262
BspMAI	CTGCAG	2 cut(s)	582, 1173
BspMI	ACCTGC	2 cut(s)	1500, 1732
BspPI	GGATC	1 cut(s)	1829
BspQI	GCTCTTC	1 cut(s)	893
BspT104I	TTCGAA	1 cut(s)	1609
BspT107I	GGYRCC	1 cut(s)	1260
BspTNI	GGTCTC	1 cut(s)	706
BsrDI	GCAATG	3 cut(s)	794, 1595, 1839
BsrFI	RCCGGY	1 cut(s)	1004
BsrI	ACTGG	5 cut(s)	324, 343, 873, 887, 1050
BssAI	RCCGGY	1 cut(s)	1004
BssECI	CCNNGG	4 cut(s)	264, 474, 1854, 2027
BssMI	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
BssNI	GRCGYC	3 cut(s)	688, 860, 2271
BssSI	CACGAG	2 cut(s)	569, 1386
BssT1I	CCWWGG	2 cut(s)	264, 2027
Bst2BI	CACGAG	2 cut(s)	569, 1386
Bst2UI	CCWGG	1 cut(s)	476
Bst4CI	ACNGT	5 cut(s)	62, 92, 1588, 1804, 2213
Bst6I	CTCTTC	3 cut(s)	893, 2145, 2243
BstACI	GRCGYC	3 cut(s)	688, 860, 2271
BstAPI	GCANNNNNTGC	1 cut(s)	1627
BstBAI	YACGTR	2 cut(s)	922, 1570
BstBI	TTCGAA	1 cut(s)	1609
BstC8I	GCNNGC	7 cut(s)	104, 199, 306, 1006, 1911, 1982, 2078
BstDSI	CCRYGG	2 cut(s)	264, 1854
BstENI	CCTNNNNNAGG	1 cut(s)	1496
BstF5I	GGATG	7 cut(s)	352, 403, 601, 1135, 1351, 2350, 2388
BstFNI	CGCG	1 cut(s)	1856
BstHHI	GCGC	2 cut(s)	913, 2218
BstKTI	GATC	8 cut(s)	156, 189, 727, 742, 1824, 1882, 1925, 2332
BstMAI	GTCTC	9 cut(s)	251, 706, 752, 797, 962, 967, 1346, 1937, 2328
BstMBI	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
BstMCI	CGRYCG	1 cut(s)	64
BstNI	CCWGG	1 cut(s)	476
BstNSI	RCATGY	2 cut(s)	1625, 2016
BstSCI	CCNGG	5 cut(s)	474, 624, 1703, 2179, 2232
BstSFI	CTRYAG	4 cut(s)	91, 578, 1169, 1641
BstSNI	TACGTA	1 cut(s)	1570
BstUI	CGCG	1 cut(s)	1856
BstV1I	GCAGC	7 cut(s)	214, 247, 547, 550, 1808, 2060, 2267
BstV2I	GAAGAC	3 cut(s)	21, 428, 1388
BstX2I	RGATCY	4 cut(s)	724, 1821, 1879, 1922
BstXI	CCANNNNNNTGG	2 cut(s)	679, 2266
BstYI	RGATCY	4 cut(s)	724, 1821, 1879, 1922
Bsu15I	ATCGAT	1 cut(s)	185
BsuRI	GGCC	7 cut(s)	624, 629, 1004, 1576, 1708, 2125, 2205
BsuTUI	ATCGAT	1 cut(s)	185
BtgI	CCRYGG	2 cut(s)	264, 1854
BtgZI	GCGATG	1 cut(s)	1199
BtsCI	GGATG	7 cut(s)	352, 403, 601, 1135, 1351, 2350, 2388
BtsI	GCAGTG	1 cut(s)	412
BtsIMutI	CAGTG	6 cut(s)	18, 412, 806, 822, 1665, 2209
BveI	ACCTGC	2 cut(s)	1500, 1732
Cac8I	GCNNGC	7 cut(s)	104, 199, 306, 1006, 1911, 1982, 2078
CaiI	CAGNNNCTG	2 cut(s)	1802, 2138
CciI	TCATGA	2 cut(s)	1848, 1872
CfoI	GCGC	2 cut(s)	913, 2218
Cfr10I	RCCGGY	1 cut(s)	1004
Cfr13I	GGNCC	2 cut(s)	627, 1706
Cfr42I	CCGCGG	1 cut(s)	1857
ClaI	ATCGAT	1 cut(s)	185
CseI	GACGC	3 cut(s)	696, 984, 2279
Csp6I	GTAC	6 cut(s)	98, 530, 870, 1261, 1784, 2043
CviQI	GTAC	6 cut(s)	98, 530, 870, 1261, 1784, 2043
DpnI	GATC	8 cut(s)	155, 188, 726, 741, 1823, 1881, 1924, 2331
DpnII	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
DriI	GACNNNNNGTC	2 cut(s)	1462, 1998
EaeI	YGGCCR	1 cut(s)	622
Eam1104I	CTCTTC	3 cut(s)	893, 2145, 2243
Eam1105I	GACNNNNNGTC	2 cut(s)	1462, 1998
EarI	CTCTTC	3 cut(s)	893, 2145, 2243
Ecl136II	GAGCTC	2 cut(s)	285, 1135
Eco105I	TACGTA	1 cut(s)	1570
Eco130I	CCWWGG	2 cut(s)	264, 2027
Eco147I	AGGCCT	1 cut(s)	1576
Eco24I	GRGCYC	2 cut(s)	287, 1137
Eco31I	GGTCTC	1 cut(s)	706
Eco32I	GATATC	3 cut(s)	1091, 1414, 2410
Eco53kI	GAGCTC	2 cut(s)	285, 1135
Eco57I	CTGAAG	2 cut(s)	759, 1517
Eco72I	CACGTG	1 cut(s)	922
EcoICRI	GAGCTC	2 cut(s)	285, 1135
EcoNI	CCTNNNNNAGG	1 cut(s)	1496
EcoRI	GAATTC	1 cut(s)	1689
EcoRII	CCWGG	1 cut(s)	474
EcoRV	GATATC	3 cut(s)	1091, 1414, 2410
EcoT14I	CCWWGG	2 cut(s)	264, 2027
EcoT38I	GRGCYC	2 cut(s)	287, 1137
ErhI	CCWWGG	2 cut(s)	264, 2027
Esp3I	CGTCTC	3 cut(s)	752, 967, 2328
FalI	AAGNNNNNCTT	4 cut(s)	992, 1024, 1944, 1976
FaqI	GGGAC	5 cut(s)	847, 1469, 2005, 2196, 2216
FauI	CCCGC	1 cut(s)	792
FauNDI	CATATG	2 cut(s)	612, 969
FbaI	TGATCA	1 cut(s)	153
FblI	GTMKAC	1 cut(s)	685
Fnu4HI	GCNGC	7 cut(s)	228, 236, 561, 564, 1797, 2074, 2256
FokI	GGATG	7 cut(s)	359, 410, 588, 1142, 1358, 2357, 2375
FriOI	GRGCYC	2 cut(s)	287, 1137
Fsp4HI	GCNGC	7 cut(s)	228, 236, 561, 564, 1797, 2074, 2256
FspBI	CTAG	3 cut(s)	116, 1028, 1071
GlaI	GCGC	2 cut(s)	912, 2217
GluI	GCNGC	7 cut(s)	228, 236, 561, 564, 1797, 2074, 2256
HaeIII	GGCC	7 cut(s)	624, 629, 1004, 1576, 1708, 2125, 2205
HapII	CCGG	5 cut(s)	625, 1005, 1704, 2180, 2234
HgaI	GACGC	3 cut(s)	696, 984, 2279
HhaI	GCGC	2 cut(s)	913, 2218
Hin1I	GRCGYC	3 cut(s)	688, 860, 2271
Hin6I	GCGC	2 cut(s)	911, 2216
HinP1I	GCGC	2 cut(s)	911, 2216
HincII	GTYRAC	2 cut(s)	1507, 2353
HindII	GTYRAC	2 cut(s)	1507, 2353
HindIII	AAGCTT	3 cut(s)	1223, 1286, 1777
HinfI	GANTC	9 cut(s)	215, 488, 515, 1056, 1113, 1354, 1362, 1592, 2135
HpaI	GTTAAC	1 cut(s)	1507
HpaII	CCGG	5 cut(s)	625, 1005, 1704, 2180, 2234
HphI	GGTGA	2 cut(s)	1481, 2396
Hpy166II	GTNNAC	5 cut(s)	40, 98, 686, 1507, 2353
Hpy8I	GTNNAC	5 cut(s)	40, 98, 686, 1507, 2353
Hpy99I	CGWCG	2 cut(s)	862, 2297
HpyAV	CCTTC	9 cut(s)	629, 814, 994, 1103, 1412, 1492, 1611, 2216, 2407
HpyCH4III	ACNGT	5 cut(s)	62, 92, 1588, 1804, 2213
HpyCH4IV	ACGT	4 cut(s)	860, 921, 1569, 2292
HpySE526I	ACGT	4 cut(s)	860, 921, 1569, 2292
Hsp92I	GRCGYC	3 cut(s)	688, 860, 2271
HspAI	GCGC	2 cut(s)	911, 2216
KpnI	GGTACC	1 cut(s)	1264
KroI	GCCGGC	1 cut(s)	1004
KroNI	GCCGGC	1 cut(s)	1006
Ksp22I	TGATCA	1 cut(s)	153
KspAI	GTTAAC	1 cut(s)	1507
KspI	CCGCGG	1 cut(s)	1857
Kzo9I	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
LguI	GCTCTTC	1 cut(s)	893
LmnI	GCTCC	3 cut(s)	918, 1858, 2365
Lsp1109I	GCAGC	7 cut(s)	214, 247, 547, 550, 1808, 2060, 2267
LweI	GCATC	7 cut(s)	509, 547, 800, 1783, 1890, 1894, 2335
MaeI	CTAG	3 cut(s)	116, 1028, 1071
MaeII	ACGT	4 cut(s)	860, 921, 1569, 2292
MaeIII	GTNAC	7 cut(s)	334, 875, 1066, 1464, 2000, 2282, 2384
MalI	GATC	8 cut(s)	155, 188, 726, 741, 1823, 1881, 1924, 2331
MboI	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
MfeI	CAATTG	1 cut(s)	510
MflI	RGATCY	4 cut(s)	724, 1821, 1879, 1922
MhlI	GDGCHC	2 cut(s)	287, 1137
MlyI	GAGTC	2 cut(s)	1065, 1348
MmeI	TCCRAC	6 cut(s)	44, 52, 881, 922, 1107, 2247
MroNI	GCCGGC	1 cut(s)	1004
MroXI	GAANNNNTTC	2 cut(s)	1200, 1224
MslI	CAYNNNNRTG	1 cut(s)	677
MspA1I	CMGCKG	1 cut(s)	1856
MspI	CCGG	5 cut(s)	625, 1005, 1704, 2180, 2234
MspR9I	CCNGG	5 cut(s)	476, 626, 1705, 2181, 2234
MunI	CAATTG	1 cut(s)	510
Mva1269I	GAATGC	1 cut(s)	2336
MvaI	CCWGG	1 cut(s)	476
MvnI	CGCG	1 cut(s)	1856
NaeI	GCCGGC	1 cut(s)	1006
NciI	CCSGG	4 cut(s)	626, 1705, 2181, 2234
NcoI	CCATGG	1 cut(s)	264
NdeI	CATATG	2 cut(s)	612, 969
NdeII	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
NgoMIV	GCCGGC	1 cut(s)	1004
NlaIV	GGNNCC	1 cut(s)	1262
NmeAIII	GCCGAG	1 cut(s)	1463
NmuCI	GTSAC	3 cut(s)	1464, 2000, 2384
NspI	RCATGY	2 cut(s)	1625, 2016
NspV	TTCGAA	1 cut(s)	1609
PagI	TCATGA	2 cut(s)	1848, 1872
PaqCI	CACCTGC	2 cut(s)	1500, 1732
PceI	AGGCCT	1 cut(s)	1576
PciI	ACATGT	1 cut(s)	2012
PciSI	GCTCTTC	1 cut(s)	893
PcsI	WCGNNNNNNNCGW	1 cut(s)	252
PctI	GAATGC	1 cut(s)	2336
PdiI	GCCGGC	1 cut(s)	1006
PdmI	GAANNNNTTC	2 cut(s)	1200, 1224
PfeI	GAWTC	7 cut(s)	215, 488, 515, 1113, 1362, 1592, 2135
PfoI	TCCNGGA	2 cut(s)	2179, 2232
PkrI	GCNGC	7 cut(s)	229, 237, 562, 565, 1798, 2075, 2257
PleI	GAGTC	2 cut(s)	1064, 1348
PmaCI	CACGTG	1 cut(s)	922
PmlI	CACGTG	1 cut(s)	922
PpsI	GAGTC	2 cut(s)	1064, 1348
Ppu21I	YACGTR	2 cut(s)	922, 1570
PscI	ACATGT	1 cut(s)	2012
Psp124BI	GAGCTC	2 cut(s)	287, 1137
Psp6I	CCWGG	1 cut(s)	474
PspCI	CACGTG	1 cut(s)	922
PspGI	CCWGG	1 cut(s)	474
PspN4I	GGNNCC	1 cut(s)	1262
PspPI	GGNCC	2 cut(s)	627, 1706
PstI	CTGCAG	2 cut(s)	582, 1173
PstNI	CAGNNNCTG	2 cut(s)	1802, 2138
PsuI	RGATCY	4 cut(s)	724, 1821, 1879, 1922
RsaI	GTAC	6 cut(s)	99, 531, 871, 1262, 1785, 2044
RsaNI	GTAC	6 cut(s)	98, 530, 870, 1261, 1784, 2043
RseI	CAYNNNNRTG	1 cut(s)	677
SacI	GAGCTC	2 cut(s)	287, 1137
SacII	CCGCGG	1 cut(s)	1857
SapI	GCTCTTC	1 cut(s)	893
SatI	GCNGC	7 cut(s)	228, 236, 561, 564, 1797, 2074, 2256
Sau3AI	GATC	8 cut(s)	153, 186, 724, 739, 1821, 1879, 1922, 2329
Sau96I	GGNCC	2 cut(s)	627, 1706
SchI	GAGTC	2 cut(s)	1065, 1348
ScrFI	CCNGG	5 cut(s)	476, 626, 1705, 2181, 2234
SduI	GDGCHC	2 cut(s)	287, 1137
SfaNI	GCATC	7 cut(s)	509, 547, 800, 1783, 1890, 1894, 2335
SfcI	CTRYAG	4 cut(s)	91, 578, 1169, 1641
Sfr303I	CCGCGG	1 cut(s)	1857
SfuI	TTCGAA	1 cut(s)	1609
SgrBI	CCGCGG	1 cut(s)	1857
SmiMI	CAYNNNNRTG	1 cut(s)	677
SmlI	CTYRAG	2 cut(s)	839, 2325
SmoI	CTYRAG	2 cut(s)	839, 2325
SnaBI	TACGTA	1 cut(s)	1570
SseBI	AGGCCT	1 cut(s)	1576
SsiI	CCGC	4 cut(s)	799, 1854, 1856, 2363
SspMI	CTAG	3 cut(s)	116, 1028, 1071
SstI	GAGCTC	2 cut(s)	287, 1137
StuI	AGGCCT	1 cut(s)	1576
StyD4I	CCNGG	5 cut(s)	474, 624, 1703, 2179, 2232
StyI	CCWWGG	2 cut(s)	264, 2027
TaaI	ACNGT	5 cut(s)	62, 92, 1588, 1804, 2213
TaiI	ACGT	4 cut(s)	863, 924, 1572, 2295
TaqI	TCGA	5 cut(s)	185, 331, 1379, 1609, 2295
TfiI	GAWTC	7 cut(s)	215, 488, 515, 1113, 1362, 1592, 2135
TscAI	CASTG	6 cut(s)	25, 412, 813, 822, 1672, 2216
TseFI	GTSAC	3 cut(s)	1464, 2000, 2384
TseI	GCWGC	7 cut(s)	227, 235, 560, 563, 1796, 2073, 2255
Tsp45I	GTSAC	3 cut(s)	1464, 2000, 2384
TspDTI	ATGAA	8 cut(s)	98, 674, 757, 1056, 1308, 1682, 1837, 1861
TspGWI	ACGGA	1 cut(s)	261
TspRI	CASTG	6 cut(s)	25, 412, 813, 822, 1672, 2216
XagI	CCTNNNNNAGG	1 cut(s)	1496
XapI	RAATTY	7 cut(s)	119, 845, 1163, 1319, 1325, 1425, 1689
XceI	RCATGY	2 cut(s)	1625, 2016
XcmI	CCANNNNNNNNNTGG	3 cut(s)	271, 397, 617
XmiI	GTMKAC	1 cut(s)	685
XmnI	GAANNNNTTC	2 cut(s)	1200, 1224
XspI	CTAG	3 cut(s)	116, 1028, 1071
ZraI	GACGTC	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.