Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002530

Physical Location & Seq

Genomic Coordinates Forward (+)

31331814 .. 31342524

Gene Structure 10711 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1214700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGAGGAGGAGATGGACGAAACCGATGCTCAACCTCAGGTGTATATGGCCTGCATTCGACACGGCCATCGTGTTGGAGTTTCTTATTACGATTCTAGTATTCGCCAGCTTCATGTGCTGGAAGTTTGGGAAGATGGCAGCACGGACTTCCCCCTAATTGATTTGGTAAAATATCAAGCCAAGCCGCTTATCATTTACACAAGTACTAAAAGTGAAGAATCATTCTTGTCCGCTTTACAGCGAAGTGATGGAATGACTGAGGCTTTTACGGTAAAGCTTGTGAAAAGTTCAATATTTAGCTATGAGCAAGCATGGCACAGATTGATATACCTTCGAGTAACAGGGATGGATGATGGATTAAACATCAAGGAGAGAATTTGCTATTTGAGTTCTATGATGGACATGGGAAGTGATGTTCAAGTTCGTACTAGTGGTGGCCTTCTTGCCATATTAGAGAATGAAAGGGTTGTAGATACGCTTGGACAAATGGAAAGTGGGAACCCATCCATTGCAATTGATTCTGTTATAGAAGTGTCACTAAATAATTTCCTTAAACTCGATGCAGCAGCTCATGAGGCACTGCAGATATTCCTAACAGATAAGCATCCAAGCCATATGGGGATTGGTAGAGCAAAAGAAGGGTTCTCTGTATTTGGCATGATGAATAAGTGTGTGACGCCAATGGGGAGACGTCTCTTGAGAAGCTGGTTCTTGAGGCCAATACTTGACCTTGAAGTTTTGAACAACCGTCTCAATGCCATATCATTCTTTCTTTCTTCTGAAGAATTGATAAATTCTTTACATGGAACTCTGAAATCTGTAAAGGACATTCCTCACATACTCAAGAAATTCAACTCCCCGAGCTCGATATGTACCAGCAGTGATTGGACAGCTTTTTTAAAGAGTGTTTGTTCACTCTTGCATGTAAACAAGATATTTGAAGTAGGTGTTTCTGAGAGTCTTCAAGAGCATGCAAAGTACTTCAATTTGGACATTGTTGAGAAGGCTGCTTCATGCATTACAACTGAGCTGGCTTATGTATATGAATTGGTAATTGGTGTTCTAGATGTAAATAGAAGTAAGGAGAAGGGATATGCGACGATTGTGAAAGATGACTTCTGTGATGAGTTAGATGAGCTGAGGCAAATATATGAGGAATTGCCTGAGTTTTTAGAACAGGTGTCTCAAATGGAACTTGAAAGACTTCCTCAGCTGTACAAAGACAAGCTTGCTCCTTGTATTGTTTATATACATCAGATAGGATACTTGATGTGCAGTTTCGAAGAAAAATTTGATGAAGCTACCCTGGAGAAAGTTCAAGATTTCGAATTTGCTTTCTCTGATGCAGAAGGAGAAACAAAAAGGTTCTTTTATTATACACCAAAGACACGAGAGCTGGATAATTTGCTGGGAGATATCTATCATAAAATACTAGATATGGAGAGGGCAATCACTCGAGATTTGGTGTCGCATATACTTGTATTTTCAGAACACCTGCTTAAGGCTGTAAATTTTGCAGCTGAACTTGATTGCTTTTTGTCATTGGCACTGGTTGCTCGTCAAAACAATTATGTGAGGCCGACGCTGACTATGGATTGCTTGATTGATATACAAAACGGAAGACATGTTTTGCAGGAAATGATTGTAGACACATTTGTTCCGAATGACACAAAGATCGAAAATGATGGAAGAATTAATATCATCACTGGTCCAAACTATTCAGGGAAAAGCATCTACATAAAGCAGGTAGCTTTAATTGTTTTCCTGTCCCACATAGGAAGTTTTGTACCAGCAGATTCTGCAACTGTTGGCTTGACTGATAGAATATTTTGCGCAATGGGAAGCAAGTTTATGAGTGCTGAAAAGTCAACATTTATGATTGATTTGCATCAAGTAGGGATGATGCTAAGGCAGGCGACTTCAAGGTCTTTGTGCCTCGTTGATGAATTTGGTAAAGGTACTCTTACAGAAGATGGTGTTGGTTTGCTTGGTGGAACTATAAACTACTTGGTTTCATATGATGACCCTCCAAAGGTACTGGTGTGCACTCATCTGACTGAGTTATTCAGTGAGAGTTGTTTACCTAAATCCAAGAACATCAAATTTTACACTATGAGTGTGTTGAGGCCTGAGAACTTTACAGATATTGAGGATATTGTATTCCTATATAGGCTAGTTCCTGGGCATGCACTTCTTAGCTACGGTCTGCATTGTGCTCTACTTGCCGGTGTCCCCGAGGAAGTTATTCAAAGAGCATCATTTATATTGGACGCTCATGGAAACAATAAACACGTTGAGCGATTGTGCAATGAGAACGTATCAGCTATGGATCAGCAATACCAGAATGTAGTGGACAAGATGCTGGCGTTTGATTTTCGCAAAGGCGATGTAGGCTTGTTTTTTCAGGATTTATTTCCAACCGAGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000710 GO:0000712 GO:0000793 GO:0000794 GO:0000795 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0010777 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0030983 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042623 GO:0043073 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0098813 GO:0099086 GO:0140013 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MutS_III MutS_IV MutS_V

Protein Analysis

810

Amino Acids

91.61

Weight (kDa)

5.06

Isoelectric Point (pI)

40.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_III	PF05192	190 - 516	9e-32	MutS domain III
MutS_V	PF00488	567 - 756	8.1e-47	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20475 AT3G20475 AT3G20475
fragaria_vesca	FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000 FvH4_3g23000
malus_domestica	MD11G1214700.v1.1
prunus_persica	Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1 Prupe.4G216500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0040391 RchiOBHm_Chr5g0040451
rosa_laevigata	RLG00000033976
rosa_multiflora	Rmu_sc0004516.1_g000007
rosa_roxburghii	Rroxscaffold_1G00040210 Rroxscaffold_1G00040250 Rroxscaffold_1G00040300 Rroxscaffold_1G00040940
rosa_rugosa	Rorug05G0184700
rosa_samantha	Rh5AG272000 Rh5BG275900 Rh5CG308000 Rh5DG284100
rosa_wichuraiana	Rw5G025430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1503
AasI	GACNNNNNNGTC	1 cut(s)	1924
AatII	GACGTC	1 cut(s)	694
Acc16I	TGCGCA	1 cut(s)	1834
Acc36I	ACCTGC	2 cut(s)	1503, 1735
AccI	GTMKAC	1 cut(s)	1647
AciI	CCGC	2 cut(s)	185, 231
AclWI	GGATC	1 cut(s)	2337
AcoI	YGGCCR	1 cut(s)	64
AcsI	RAATTY	8 cut(s)	375, 793, 848, 1289, 1328, 1510, 1946, 2102
AcuI	CTGAAG	1 cut(s)	801
AcyI	GRCGYC	2 cut(s)	677, 691
AfaI	GTAC	8 cut(s)	205, 427, 874, 980, 1217, 1788, 1960, 2037
AfiI	CCNNNNNNNGG	2 cut(s)	1501, 2032
AflII	CTTAAG	1 cut(s)	1499
AflIII	ACRYGT	2 cut(s)	1624, 2290
AhlI	ACTAGT	1 cut(s)	428
AjnI	CCWGG	2 cut(s)	1305, 2179
AjuI	GAANNNNNNNTTGG	4 cut(s)	1038, 1070, 1962, 1994
AloI	GAACNNNNNNTCC	2 cut(s)	399, 431
Alw21I	GWGCWC	3 cut(s)	866, 2048, 2218
Alw26I	GTCTC	4 cut(s)	682, 698, 755, 1188
Alw44I	GTGCAC	1 cut(s)	2044
AlwI	GGATC	1 cut(s)	2337
AlwNI	CAGNNNCTG	1 cut(s)	1799
Ama87I	CYCGRG	3 cut(s)	859, 1455, 2234
AoxI	GGCC	6 cut(s)	48, 64, 436, 716, 1577, 2126
ApaLI	GTGCAC	1 cut(s)	2044
ApeKI	GCWGC	5 cut(s)	138, 563, 566, 1007, 1517
ApoI	RAATTY	8 cut(s)	375, 793, 848, 1289, 1328, 1510, 1946, 2102
AseI	ATTAAT	1 cut(s)	1695
Asp700I	GAANNNNTTC	2 cut(s)	1203, 2244
AspLEI	GCGC	1 cut(s)	1835
AspS9I	GGNCC	1 cut(s)	1709
AsuII	TTCGAA	2 cut(s)	1281, 1326
AvaI	CYCGRG	3 cut(s)	859, 1455, 2234
AvaII	GGWCC	1 cut(s)	1709
AxyI	CCTNAGG	1 cut(s)	36
BaeGI	GKGCMC	1 cut(s)	2048
BaeI	ACNNNNGTAYC	2 cut(s)	2027, 2060
BanII	GRGCYC	1 cut(s)	866
BauI	CACGAG	1 cut(s)	1389
BbsI	GAAGAC	2 cut(s)	953, 1627
Bbv12I	GWGCWC	3 cut(s)	866, 2048, 2218
BbvCI	CCTCAGC	2 cut(s)	1139, 1209
BbvI	GCAGC	5 cut(s)	150, 575, 578, 994, 1529
BceAI	ACGGC	1 cut(s)	79
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BciT130I	CCWGG	2 cut(s)	1307, 2181
BciVI	GTATCC	1 cut(s)	1256
BcoDI	GTCTC	4 cut(s)	682, 698, 755, 1188
BcuI	ACTAGT	1 cut(s)	428
BfaI	CTAG	5 cut(s)	96, 429, 1064, 1433, 2174
BfmI	CTRYAG	1 cut(s)	581
BfrI	CTTAAG	1 cut(s)	1499
BfuAI	ACCTGC	2 cut(s)	1503, 1735
BfuI	GTATCC	1 cut(s)	1256
BisI	GCNGC	6 cut(s)	139, 185, 564, 567, 1008, 1518
BlsI	GCNGC	6 cut(s)	140, 186, 565, 568, 1009, 1519
BmcAI	AGTACT	2 cut(s)	205, 980
Bme1390I	CCNGG	2 cut(s)	1307, 2181
Bme18I	GGWCC	1 cut(s)	1709
BmeT110I	CYCGRG	3 cut(s)	859, 1455, 2234
BmgT120I	GGNCC	1 cut(s)	1709
BmiI	GGNNCC	1 cut(s)	500
BmrFI	CCNGG	2 cut(s)	1307, 2181
BmsI	GCATC	9 cut(s)	16, 550, 613, 1333, 1740, 1893, 1897, 2264, 2349
BpiI	GAAGAC	2 cut(s)	953, 1627
BpmI	CTGGAG	1 cut(s)	1328
Bpu10I	CCTNAGC	3 cut(s)	1139, 1209, 1907
Bpu14I	TTCGAA	2 cut(s)	1281, 1326
BpuEI	CTTGAG	3 cut(s)	718, 733, 827
BsaBI	GATNNNNATC	3 cut(s)	603, 1419, 1887
BsaHI	GRCGYC	2 cut(s)	677, 691
BsaJI	CCNNGG	3 cut(s)	1305, 2180, 2235
Bsc4I	CCNNNNNNNGG	2 cut(s)	1501, 2032
Bse118I	RCCGGY	1 cut(s)	2225
Bse1I	ACTGG	3 cut(s)	1554, 1711, 2043
Bse21I	CCTNAGG	1 cut(s)	36
Bse3DI	GCAATG	3 cut(s)	507, 1842, 2314
Bse8I	GATNNNNATC	3 cut(s)	603, 1419, 1887
BseBI	CCWGG	2 cut(s)	1307, 2181
BseDI	CCNNGG	3 cut(s)	1305, 2180, 2235
BseGI	GGATG	5 cut(s)	351, 355, 503, 604, 1905
BseJI	GATNNNNATC	3 cut(s)	603, 1419, 1887
BseLI	CCNNNNNNNGG	2 cut(s)	1501, 2032
BseMI	GCAATG	3 cut(s)	507, 1842, 2314
BseMII	CTCAG	9 cut(s)	50, 249, 945, 1017, 1130, 1155, 1223, 2049, 2121
BseNI	ACTGG	3 cut(s)	1554, 1711, 2043
BseRI	GAGGAG	2 cut(s)	20, 23
BseSI	GKGCMC	1 cut(s)	2048
BseXI	GCAGC	5 cut(s)	150, 575, 578, 994, 1529
BseYI	CCCAGC	1 cut(s)	1408
BsgI	GTGCAG	1 cut(s)	1294
BshFI	GGCC	6 cut(s)	50, 66, 438, 718, 1579, 2128
BsiHKAI	GWGCWC	3 cut(s)	866, 2048, 2218
BsiHKCI	CYCGRG	3 cut(s)	859, 1455, 2234
BsiSI	CCGG	1 cut(s)	2226
BslFI	GGGAC	2 cut(s)	1753, 2216
BslI	CCNNNNNNNGG	2 cut(s)	1501, 2032
BsmAI	GTCTC	4 cut(s)	682, 698, 755, 1188
BsmBI	CGTCTC	3 cut(s)	682, 698, 755
BsmFI	GGGAC	2 cut(s)	1753, 2216
BsmI	GAATGC	1 cut(s)	54
BsnI	GGCC	6 cut(s)	50, 66, 438, 718, 1579, 2128
BsoBI	CYCGRG	3 cut(s)	859, 1455, 2234
Bsp119I	TTCGAA	2 cut(s)	1281, 1326
Bsp1286I	GDGCHC	3 cut(s)	866, 2048, 2218
Bsp1407I	TGTACA	1 cut(s)	1215
Bsp143I	GATC	2 cut(s)	1674, 2329
BspACI	CCGC	2 cut(s)	185, 231
BspANI	GGCC	6 cut(s)	50, 66, 438, 718, 1579, 2128
BspCNI	CTCAG	9 cut(s)	49, 250, 946, 1018, 1131, 1156, 1222, 2050, 2122
BspHI	TCATGA	1 cut(s)	571
BspLI	GGNNCC	1 cut(s)	500
BspMAI	CTGCAG	1 cut(s)	585
BspMI	ACCTGC	2 cut(s)	1503, 1735
BspPI	GGATC	1 cut(s)	2337
BspT104I	TTCGAA	2 cut(s)	1281, 1326
BspTI	CTTAAG	1 cut(s)	1499
BsrDI	GCAATG	3 cut(s)	507, 1842, 2314
BsrFI	RCCGGY	1 cut(s)	2225
BsrGI	TGTACA	1 cut(s)	1215
BsrI	ACTGG	3 cut(s)	1554, 1711, 2043
BssAI	RCCGGY	1 cut(s)	2225
BssECI	CCNNGG	3 cut(s)	1305, 2180, 2235
BssMI	GATC	2 cut(s)	1674, 2329
BssNI	GRCGYC	2 cut(s)	677, 691
BssSI	CACGAG	1 cut(s)	1389
Bst2BI	CACGAG	1 cut(s)	1389
Bst2UI	CCWGG	2 cut(s)	1307, 2181
Bst4CI	ACNGT	4 cut(s)	271, 749, 1807, 2204
BstACI	GRCGYC	2 cut(s)	677, 691
BstAFI	CTTAAG	1 cut(s)	1499
BstAPI	GCANNNNNTGC	2 cut(s)	1553, 1799
BstAUI	TGTACA	1 cut(s)	1215
BstBI	TTCGAA	2 cut(s)	1281, 1326
BstC8I	GCNNGC	9 cut(s)	52, 107, 309, 972, 1032, 1230, 1914, 2187, 2364
BstENI	CCTNNNNNAGG	1 cut(s)	1499
BstF5I	GGATG	5 cut(s)	351, 355, 503, 604, 1905
BstHHI	GCGC	1 cut(s)	1835
BstKTI	GATC	2 cut(s)	1677, 2332
BstMAI	GTCTC	4 cut(s)	682, 698, 755, 1188
BstMBI	GATC	2 cut(s)	1674, 2329
BstMWI	GCNNNNNNNGC	7 cut(s)	115, 313, 575, 1553, 1799, 2222, 2391
BstNI	CCWGG	2 cut(s)	1307, 2181
BstNSI	RCATGY	4 cut(s)	926, 974, 1628, 2189
BstSCI	CCNGG	2 cut(s)	1305, 2179
BstSFI	CTRYAG	1 cut(s)	581
BstSLI	GKGCMC	1 cut(s)	2048
BstV1I	GCAGC	5 cut(s)	150, 575, 578, 994, 1529
BstV2I	GAAGAC	2 cut(s)	953, 1627
BstXI	CCANNNNNNTGG	1 cut(s)	74
Bsu36I	CCTNAGG	1 cut(s)	36
BsuI	GTATCC	1 cut(s)	1256
BsuRI	GGCC	6 cut(s)	50, 66, 438, 718, 1579, 2128
BtgZI	GCGATG	1 cut(s)	2400
BtsCI	GGATG	5 cut(s)	351, 355, 503, 604, 1905
BtsI	GCAGTG	2 cut(s)	578, 886
BtsIMutI	CAGTG	5 cut(s)	578, 886, 1547, 1704, 2074
BveI	ACCTGC	2 cut(s)	1503, 1735
Cac8I	GCNNGC	9 cut(s)	52, 107, 309, 972, 1032, 1230, 1914, 2187, 2364
CaiI	CAGNNNCTG	1 cut(s)	1799
CciI	TCATGA	1 cut(s)	571
CfoI	GCGC	1 cut(s)	1835
Cfr10I	RCCGGY	1 cut(s)	2225
Cfr13I	GGNCC	1 cut(s)	1709
CseI	GACGC	3 cut(s)	685, 1591, 2279
Csp6I	GTAC	8 cut(s)	204, 426, 873, 979, 1216, 1787, 1959, 2036
CviQI	GTAC	8 cut(s)	204, 426, 873, 979, 1216, 1787, 1959, 2036
DpnI	GATC	2 cut(s)	1676, 2331
DpnII	GATC	2 cut(s)	1674, 2329
DraI	TTTAAA	1 cut(s)	900
DrdI	GACNNNNNNGTC	1 cut(s)	1924
DseDI	GACNNNNNNGTC	1 cut(s)	1924
EaeI	YGGCCR	1 cut(s)	64
Ecl136II	GAGCTC	1 cut(s)	864
Eco147I	AGGCCT	1 cut(s)	2128
Eco24I	GRGCYC	1 cut(s)	866
Eco32I	GATATC	1 cut(s)	1417
Eco47I	GGWCC	1 cut(s)	1709
Eco53kI	GAGCTC	1 cut(s)	864
Eco57I	CTGAAG	1 cut(s)	801
Eco81I	CCTNAGG	1 cut(s)	36
Eco88I	CYCGRG	3 cut(s)	859, 1455, 2234
EcoICRI	GAGCTC	1 cut(s)	864
EcoNI	CCTNNNNNAGG	1 cut(s)	1499
EcoRII	CCWGG	2 cut(s)	1305, 2179
EcoRV	GATATC	1 cut(s)	1417
EcoT22I	ATGCAT	1 cut(s)	1019
EcoT38I	GRGCYC	1 cut(s)	866
Esp3I	CGTCTC	3 cut(s)	682, 698, 755
FalI	AAGNNNNNCTT	4 cut(s)	1212, 1244, 1947, 1979
FaqI	GGGAC	2 cut(s)	1753, 2216
FauNDI	CATATG	2 cut(s)	615, 2017
FblI	GTMKAC	1 cut(s)	1647
Fnu4HI	GCNGC	6 cut(s)	139, 185, 564, 567, 1008, 1518
FokI	GGATG	5 cut(s)	358, 362, 490, 591, 1912
FriOI	GRGCYC	1 cut(s)	866
Fsp4HI	GCNGC	6 cut(s)	139, 185, 564, 567, 1008, 1518
FspBI	CTAG	5 cut(s)	96, 429, 1064, 1433, 2174
FspI	TGCGCA	1 cut(s)	1834
GlaI	GCGC	1 cut(s)	1834
GluI	GCNGC	6 cut(s)	139, 185, 564, 567, 1008, 1518
GsaI	CCCAGC	1 cut(s)	1412
GsuI	CTGGAG	1 cut(s)	1328
HaeIII	GGCC	6 cut(s)	50, 66, 438, 718, 1579, 2128
HapII	CCGG	1 cut(s)	2226
HgaI	GACGC	3 cut(s)	685, 1591, 2279
HhaI	GCGC	1 cut(s)	1835
Hin1I	GRCGYC	2 cut(s)	677, 691
Hin6I	GCGC	1 cut(s)	1833
HinP1I	GCGC	1 cut(s)	1833
HincII	GTYRAC	1 cut(s)	1869
HindII	GTYRAC	1 cut(s)	1869
HindIII	AAGCTT	2 cut(s)	275, 1226
HinfI	GANTC	5 cut(s)	92, 218, 518, 958, 1796
HpaII	CCGG	1 cut(s)	2226
Hpy166II	GTNNAC	7 cut(s)	914, 928, 1648, 1869, 2046, 2081, 2353
Hpy188I	TCNGA	8 cut(s)	781, 813, 955, 1257, 1342, 1489, 1662, 2055
Hpy188III	TCNNGA	9 cut(s)	572, 697, 712, 844, 965, 1064, 1319, 1457, 2405
Hpy8I	GTNNAC	7 cut(s)	914, 928, 1648, 1869, 2046, 2081, 2353
Hpy99I	CGWCG	2 cut(s)	1102, 1585
HpyAV	CCTTC	6 cut(s)	341, 449, 632, 997, 1081, 1343
HpyCH4III	ACNGT	4 cut(s)	271, 749, 1807, 2204
HpyCH4IV	ACGT	3 cut(s)	691, 2292, 2316
HpyF10VI	GCNNNNNNNGC	7 cut(s)	115, 313, 575, 1553, 1799, 2222, 2391
HpySE526I	ACGT	3 cut(s)	691, 2292, 2316
Hsp92I	GRCGYC	2 cut(s)	677, 691
HspAI	GCGC	1 cut(s)	1833
Kzo9I	GATC	2 cut(s)	1674, 2329
LmnI	GCTCC	1 cut(s)	1237
Lsp1109I	GCAGC	5 cut(s)	150, 575, 578, 994, 1529
LweI	GCATC	9 cut(s)	16, 550, 613, 1333, 1740, 1893, 1897, 2264, 2349
MaeI	CTAG	5 cut(s)	96, 429, 1064, 1433, 2174
MaeII	ACGT	3 cut(s)	691, 2292, 2316
MaeIII	GTNAC	3 cut(s)	337, 534, 673
MalI	GATC	2 cut(s)	1676, 2331
MboI	GATC	2 cut(s)	1674, 2329
MboII	GAAGA	9 cut(s)	143, 227, 768, 794, 953, 1295, 1632, 1701, 1982
MfeI	CAATTG	1 cut(s)	513
MhlI	GDGCHC	3 cut(s)	866, 2048, 2218
MlyI	GAGTC	1 cut(s)	967
MmeI	TCCRAC	1 cut(s)	55
Mph1103I	ATGCAT	1 cut(s)	1019
MroXI	GAANNNNTTC	2 cut(s)	1203, 2244
MseI	TTAA	7 cut(s)	359, 552, 899, 1500, 1695, 1754, 2428
MspA1I	CMGCKG	2 cut(s)	1213, 1520
MspCI	CTTAAG	1 cut(s)	1499
MspI	CCGG	1 cut(s)	2226
MspR9I	CCNGG	2 cut(s)	1307, 2181
MunI	CAATTG	1 cut(s)	513
Mva1269I	GAATGC	1 cut(s)	54
MvaI	CCWGG	2 cut(s)	1307, 2181
MwoI	GCNNNNNNNGC	7 cut(s)	115, 313, 575, 1553, 1799, 2222, 2391
NdeI	CATATG	2 cut(s)	615, 2017
NdeII	GATC	2 cut(s)	1674, 2329
NlaIV	GGNNCC	1 cut(s)	500
NmuCI	GTSAC	2 cut(s)	534, 673
NsbI	TGCGCA	1 cut(s)	1834
NsiI	ATGCAT	1 cut(s)	1019
NspI	RCATGY	4 cut(s)	926, 974, 1628, 2189
NspV	TTCGAA	2 cut(s)	1281, 1326
PaeI	GCATGC	2 cut(s)	974, 2189
PaeR7I	CTCGAG	1 cut(s)	1455
PagI	TCATGA	1 cut(s)	571
PaqCI	CACCTGC	1 cut(s)	1503
PceI	AGGCCT	1 cut(s)	2128
PciI	ACATGT	1 cut(s)	1624
PctI	GAATGC	1 cut(s)	54
PdmI	GAANNNNTTC	2 cut(s)	1203, 2244
PfeI	GAWTC	4 cut(s)	92, 218, 518, 1796
PkrI	GCNGC	6 cut(s)	140, 186, 565, 568, 1009, 1519
PleI	GAGTC	1 cut(s)	966
PpsI	GAGTC	1 cut(s)	966
PscI	ACATGT	1 cut(s)	1624
PshBI	ATTAAT	1 cut(s)	1695
Psp124BI	GAGCTC	1 cut(s)	866
Psp6I	CCWGG	2 cut(s)	1305, 2179
PspFI	CCCAGC	1 cut(s)	1408
PspGI	CCWGG	2 cut(s)	1305, 2179
PspN4I	GGNNCC	1 cut(s)	500
PspPI	GGNCC	1 cut(s)	1709
PsrI	GAACNNNNNNTAC	2 cut(s)	1044, 1076
PstI	CTGCAG	1 cut(s)	585
PstNI	CAGNNNCTG	1 cut(s)	1799
PvuII	CAGCTG	2 cut(s)	1213, 1520
RsaI	GTAC	8 cut(s)	205, 427, 874, 980, 1217, 1788, 1960, 2037
RsaNI	GTAC	8 cut(s)	204, 426, 873, 979, 1216, 1787, 1959, 2036
SacI	GAGCTC	1 cut(s)	866
SaqAI	TTAA	7 cut(s)	359, 552, 899, 1500, 1695, 1754, 2428
SatI	GCNGC	6 cut(s)	139, 185, 564, 567, 1008, 1518
Sau3AI	GATC	2 cut(s)	1674, 2329
Sau96I	GGNCC	1 cut(s)	1709
ScaI	AGTACT	2 cut(s)	205, 980
SchI	GAGTC	1 cut(s)	967
ScrFI	CCNGG	2 cut(s)	1307, 2181
SduI	GDGCHC	3 cut(s)	866, 2048, 2218
SfaNI	GCATC	9 cut(s)	16, 550, 613, 1333, 1740, 1893, 1897, 2264, 2349
SfcI	CTRYAG	1 cut(s)	581
Sfr274I	CTCGAG	1 cut(s)	1455
SfuI	TTCGAA	2 cut(s)	1281, 1326
SinI	GGWCC	1 cut(s)	1709
SlaI	CTCGAG	1 cut(s)	1455
SmlI	CTYRAG	5 cut(s)	697, 712, 842, 1455, 1499
SmoI	CTYRAG	5 cut(s)	697, 712, 842, 1455, 1499
SpeI	ACTAGT	1 cut(s)	428
SphI	GCATGC	2 cut(s)	974, 2189
SseBI	AGGCCT	1 cut(s)	2128
SsiI	CCGC	2 cut(s)	185, 231
SspI	AATATT	2 cut(s)	294, 1827
SspMI	CTAG	5 cut(s)	96, 429, 1064, 1433, 2174
SstI	GAGCTC	1 cut(s)	866
StuI	AGGCCT	1 cut(s)	2128
StyD4I	CCNGG	2 cut(s)	1305, 2179
TaaI	ACNGT	4 cut(s)	271, 749, 1807, 2204
TaiI	ACGT	3 cut(s)	694, 2295, 2319
TaqI	TCGA	8 cut(s)	58, 334, 558, 866, 1281, 1326, 1456, 1677
TatI	WGTACW	3 cut(s)	203, 978, 1215
TauI	GCSGC	1 cut(s)	187
TfiI	GAWTC	4 cut(s)	92, 218, 518, 1796
Tru1I	TTAA	7 cut(s)	359, 552, 899, 1500, 1695, 1754, 2428
Tru9I	TTAA	7 cut(s)	359, 552, 899, 1500, 1695, 1754, 2428
TscAI	CASTG	5 cut(s)	585, 886, 1554, 1711, 2074
TseFI	GTSAC	2 cut(s)	534, 673
TseI	GCWGC	5 cut(s)	138, 563, 566, 1007, 1517
Tsp45I	GTSAC	2 cut(s)	534, 673
TspDTI	ATGAA	8 cut(s)	101, 474, 677, 1002, 1059, 1311, 1959, 2004
TspGWI	ACGGA	2 cut(s)	158, 1632
TspRI	CASTG	5 cut(s)	585, 886, 1554, 1711, 2074
Vha464I	CTTAAG	1 cut(s)	1499
VneI	GTGCAC	1 cut(s)	2044
VpaK11BI	GGWCC	1 cut(s)	1709
VspI	ATTAAT	1 cut(s)	1695
XagI	CCTNNNNNAGG	1 cut(s)	1499
XapI	RAATTY	8 cut(s)	375, 793, 848, 1289, 1328, 1510, 1946, 2102
XbaI	TCTAGA	1 cut(s)	1063
XceI	RCATGY	4 cut(s)	926, 974, 1628, 2189
XcmI	CCANNNNNNNNNTGG	1 cut(s)	620
XhoI	CTCGAG	1 cut(s)	1455
XmiI	GTMKAC	1 cut(s)	1647
XmnI	GAANNNNTTC	2 cut(s)	1203, 2244
XspI	CTAG	5 cut(s)	96, 429, 1064, 1433, 2174
ZraI	GACGTC	1 cut(s)	692
ZrmI	AGTACT	2 cut(s)	205, 980
Zsp2I	ATGCAT	1 cut(s)	1019

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.