AT3G20720

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007959
Physical Location & Seq
Forward (+)
7239896 .. 7247030
7135 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20720.2

Sequence Viewer

Length: 3369 bp
ATGGAGTCGATACTTGCTCGAGCCTTGGAGTATACACTCAAGTATTGGCTCAAATCGTTTACCAGAGATCAATTTAAGCTTCAAGGCCGTACCGCTCAGCTTTCTAATTTAGACATCAATGGTGAGGCTATACATGCCAGTATGGGTTTGCCACCAGCTCTTAGTGTTACCACTGCCAAAGTTGGGAAATTGGAAATCATGCTTCCGTATGTCAGTAATGTACAAACAGAACCCATAGTTGTGCAAATCGATAAACTTGATCTTGTTCTTGAAGAAAATCCTGATGCCGATGTGACAAAGGGTCCAAGCAGTAGTCAGTCTCCAACCGCCTCTGCAAAAAGTAATGGTTATGGATTCGCCGACAAGATTGCAGATGGAATGACTTTACAAGTTAAGGTGGTCAATCTTCTTCTTGAAACTGGAGGTGGTGCCAACCGTGAAGGAGGGGCAGCCTGGGCTGCTCCGCTGGCATCTATTACAATTCGTAACCTTGTTCTGTACACCACTAATGAGAGTTGGAAGGTTGTAAATCTGAAAGAAGCAAGGGACTTCTCTACTAACACAGGATTTATTTATTTGTTTAAGAAACTTGAATGGGAAGCTTTATCAATTGATCTTCTACCACATCCTGATATGTTCACTGAAGCTAATTTAGCCCGGTCTGAAGAAGCAAATCTCAGAGATGAGGATGGTGCAAAACGAATCACGGTTCAGAGAACAGCACTAAACAGTCCACTTGGGCTTGAAGTCCAGTTGCACATTCCAGAAGCTGTTTGTCCAGCCTTAAGTGAACCAGGGTTGCGTGCTCTTCTCCGCTTTCTAACTGGAATGTACTTATGCCTAAACCGAGGAGATGTGGATCCAAAATCTCAGCAGTCTGCTGAAGCAGCTGGACGTTCTCTTGTCTCCGTTCTTGTGGATCATGTATTTCTTTGCATTAAAGATGCTGAATTCCAACTGGAACTGCTGATGCAATCACTCCTTTTTTCTCGGGCCTGTGTTTCAGACGGAGAATCAGCTAACTATTTAACTAAGATTTTGATTGGTGGGCTATTTTTAAGGGATGCTTTTTCGCGCTCTCCTTGTGCTCTAATCCAACCATCCATGAAAGCTGCTGCCGAAGACCTTGCAATTCCCGACTTTGCCAAGAACTTTTGCCCTCTCATATATCCATTGGACAGCGGCCCGTGGCAAATAGTTCAGGATGTACCCTTGATCTCCCTACACTCTCTTCAGGTCAAGCCATCCCCAAAACCCCCACACTTTTTCTCAAAGACAGTTATCCAGTGCCAACCCCTCATGGTTCATCTTCAGGAAGAAGCTTGTTTAAGAATTTCTTCTTTTCTGGCTGATGGCATTGTTGTAAATCCTGGTGATGTTTTACCTGATAACTCTGTTAACTCTCTTCTTTTCACCCTCAAAGAGTTAGATGTCTCTGTTCCATTAGACATGAGTAATTTGCAAGATTCAGCAATTGAAGAAGACCTATCCGTAAAAAAGTCCTTCGTTGGAGCGAGGCTTCATATTGAAAACTTGTCTTTTGCGGAGTCACCTACCCTAAAAGTTCGGCTACTAAACCTGGAGAAGGATCCTGCTTGCTTCTGTCTTTGGCCAGGACAGCCTATTGACGCCAGCCAAAAGAAATGGACAGCCGGAGCCTCACATTTTAGTTTGGCTTTAGAAACATCTCCCAACTCAACTCAACTTCAGAGTCCTCGTGGTCCGGAAATGGGTTTGTGGAATTGTGTTGAAGGAAAAGACGTGTCTATAGAAGTTGCTATGGTATCTGCGGATGGAAAACCTCTGATAACAATTCCCCCTCCTGGTGGTATTGTTCGCATAGGAGTTGCTTGTGAGCAATATATTTCACGTGCTTCAGTGGAGCAGTTATTTTTTGTTCTGGACCTCTATTCTTACTTTGGTAAGGTGAGTGAAAAGATTTCAATTGTTAAAGAAAGCAAACGCCAGAATACTGTTTCTTTGACTGGAGGGTTGTTGGAGAAGGTTCCTAGTGATACTGCTGTAAAATTGGCTTTGAAGGATCTGCAGCTTAAGTTTCTTGAGTCCTCGTTTACTAGTACCCAGGACATGCCACTCGTTCAGTTTCTTGGCAAAGATCTTTCTGTGAAGGTAACACATAGAACCCTTGGCGGCGCTATTGCAGTATCATCCAACATATACTGGGAAAATATTGAGGTAGATTGTGTAGATACCGACGTAGAACATGAACATGAGAATAGCTGGAACGGTCACTTAGTTAGCTGTAATGGGTCTACCCCACTGAGGCGTGTCTTTTGGGTTGTCAACGGAAGACATGATGAGCATAGTGGCAGCACTCTTACACCTTTTCTGGACATAAGCATAACACATGTGATTCCCCTCAGTGAAAAGGATATGGAGTGCCACAGTGTAAGCATCGTGGCTTATGGAACTCCAGGAAACTGGAATGGCGATGGTTTCCCACATTTGGGAAGGCCAGATGACATTGATGTCTCTGTTGAGTTAAGAGATTGGTTGTTTGCTTTGGAGGGCAGAGAAGGTGTTGGCACAAGAATTTTGAATAATGAAGATATTGGAAGAGAAGAGAGGTGTTGGCACACAAATTTCCGTACTTTCCGAGTAATAGCCAAGAGTACACCAAAGAACGTGGATTCAAATGGGACAGAAAACCAGTGTGATGCTCACAAGTATCCTGTGGACTCAATTATTGTCAGTGTCGAAGGTCTACAGACAGTGAAGCCTCAAATGCAGAAAGGGACCGATTCTTGTAATGGTTTGTCTACAAATGGAGTTCACGAAAACGGTCAAATGCATGGAGGGGTGAACATTGAAGCTAATATTGTGGCATCTGAAGACAAATCTGTTCATGATGACTTATTGAACTGGGTGGCAGAAAGTCTGAAATTCTCTGTTAAGCAACCTGTTGAGGCAGTTGTAACAAAGGATGAGCTGCAACATTTGACTTTCTTGTGCAAATCTGAAATTGACGCAATGGGAAGGATAGTCGCAGGGGTTTTACGTGTACTGAAGCTTGAAGAATCTATTGGCCAGGCGACTCTTAATCAATTAAGCAACCTTGGAAGTGAAGGTTTTGACAAGATGTTCTCTCCAAAAGCAAGCAGAGCAGGAAGTCCAAAAAGCTCACCTTTTGCTGCATCATTAGATTCAATGAGAGAAATTTCTTTACGTGCAAACCTCGAATCTACCATTTCGTCCATCGAAGAAGCCTCAATGGAGTTAGAGGCCAAGTGTTCAGCTCTAGTGTCTGATCTCAACGATTCAGAATCTTCAGCAAAACACGCCAACGAGCTCAAGCAGAAACTCGAGAGCTTGCAGAGCTTAATGGCTAAATTACGAACCCAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1122

Amino Acids

122.85

Weight (kDa)

5.19

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 32 - 205 6.8e-11 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2489
AccB1I GGYRCC 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 95
AccI GTMKAC 4 cut(s) 32, 2273, 2725, 2780
AccII CGCG 1 cut(s) 1075
AccIII TCCGGA 1 cut(s) 1723
AciI CCGC 8 cut(s) 93, 327, 464, 814, 1182, 1544, 1790, 2151
AclWI GGATC 6 cut(s) 854, 867, 927, 1583, 1596, 2049
AcoI YGGCCR 2 cut(s) 1610, 3046
AcsI RAATTY 6 cut(s) 950, 1332, 2553, 2602, 2903, 3177
AcvI CACGTG 1 cut(s) 1871
AcyI GRCGYC 1 cut(s) 1629
AfaI GTAC 9 cut(s) 91, 222, 500, 833, 1209, 2080, 2611, 2635, 3024
AfiI CCNNNNNNNGG 8 cut(s) 183, 1585, 1730, 1823, 1826, 2283, 2467, 2468
AflII CTTAAG 2 cut(s) 784, 2051
AflIII ACRYGT 3 cut(s) 1761, 2368, 3019
AhdI GACNNNNNGTC 1 cut(s) 300
AhlI ACTAGT 1 cut(s) 2075
AjiI CACGTC 1 cut(s) 1762
AjnI CCWGG 9 cut(s) 452, 793, 1369, 1578, 1612, 1822, 2082, 2434, 3048
AjuI GAANNNNNNNTTGG 2 cut(s) 3027, 3059
Alw21I GWGCWC 3 cut(s) 808, 1090, 3312
Alw26I GTCTC 4 cut(s) 324, 910, 1438, 2497
AlwI GGATC 6 cut(s) 854, 867, 927, 1583, 1596, 2049
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 3 cut(s) 18, 990, 3323
Aor13HI TCCGGA 1 cut(s) 1723
AoxI GGCC 7 cut(s) 85, 993, 1183, 1610, 2474, 3046, 3243
ApeKI GCWGC 9 cut(s) 449, 458, 887, 1112, 1115, 2047, 2331, 2950, 3152
ApoI RAATTY 6 cut(s) 950, 1332, 2553, 2602, 2903, 3177
Asp700I GAANNNNTTC 1 cut(s) 1336
AspLEI GCGC 2 cut(s) 1077, 2156
AspS9I GGNCC 6 cut(s) 302, 993, 1184, 1721, 1903, 2757
AsuC2I CCSGG 1 cut(s) 658
AsuHPI GGTGA 7 cut(s) 134, 1385, 1405, 1542, 1939, 2833, 3135
AvaI CYCGRG 3 cut(s) 18, 990, 3323
AvaII GGWCC 4 cut(s) 302, 1721, 1903, 2757
BaeI ACNNNNGTAYC 4 cut(s) 2007, 2040, 2202, 2235
BalI TGGCCA 2 cut(s) 1612, 3048
BamHI GGATCC 2 cut(s) 859, 1588
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 3312
BauI CACGAG 1 cut(s) 1716
BbrPI CACGTG 1 cut(s) 1871
BbsI GAAGAC 4 cut(s) 1128, 1488, 2317, 2859
Bbv12I GWGCWC 3 cut(s) 808, 1090, 3312
BbvI GCAGC 9 cut(s) 445, 461, 899, 1099, 1102, 2059, 2343, 2937, 3139
BccI CCATC 8 cut(s) 368, 683, 1108, 1252, 1346, 1787, 2447, 3224
BceAI ACGGC 1 cut(s) 72
BcgI CGANNNNNNTGC 4 cut(s) 350, 384, 1109, 1143
BciT130I CCWGG 9 cut(s) 454, 795, 1371, 1580, 1614, 1824, 2084, 2436, 3050
BciVI GTATCC 1 cut(s) 2700
BcnI CCSGG 1 cut(s) 658
BcoDI GTCTC 4 cut(s) 324, 910, 1438, 2497
BcuI ACTAGT 1 cut(s) 2075
BfaI CTAG 3 cut(s) 2010, 2076, 3260
BfmI CTRYAG 3 cut(s) 1767, 2045, 2726
BfoI RGCGCY 1 cut(s) 2157
BfrI CTTAAG 2 cut(s) 784, 2051
BfuI GTATCC 1 cut(s) 2700
BglII AGATCT 1 cut(s) 2116
BlpI GCTNAGC 1 cut(s) 96
Bme18I GGWCC 4 cut(s) 302, 1721, 1903, 2757
BmeRI GACNNNNNGTC 1 cut(s) 300
BmeT110I CYCGRG 3 cut(s) 18, 990, 3323
BmgBI CACGTC 1 cut(s) 1762
BmgT120I GGNCC 6 cut(s) 302, 993, 1184, 1721, 1903, 2757
BmiI GGNNCC 7 cut(s) 303, 430, 861, 1590, 1657, 2007, 2758
BmrI ACTGGG 2 cut(s) 2191, 2893
BmsI GCATC 9 cut(s) 274, 479, 934, 960, 1054, 2424, 2668, 2855, 3164
BmuI ACTGGG 2 cut(s) 2191, 2893
BpiI GAAGAC 4 cut(s) 1128, 1488, 2317, 2859
BpmI CTGGAG 4 cut(s) 441, 1601, 2007, 2418
Bpu1102I GCTNAGC 1 cut(s) 96
BpuEI CTTGAG 3 cut(s) 23, 2081, 3296
BpuMI CCSGG 1 cut(s) 658
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 3 cut(s) 1871, 3020, 3188
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 1629
BsaJI CCNNGG 8 cut(s) 24, 453, 794, 847, 1187, 2082, 2146, 3076
BsaWI WCCGGW 1 cut(s) 1723
Bsc4I CCNNNNNNNGG 8 cut(s) 183, 1585, 1730, 1823, 1826, 2283, 2467, 2468
Bse3DI GCAATG 1 cut(s) 2997
Bse8I GATNNNNATC 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 1723
BseBI CCWGG 9 cut(s) 454, 795, 1371, 1580, 1614, 1824, 2084, 2436, 3050
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 8 cut(s) 24, 453, 794, 847, 1187, 2082, 2146, 3076
BseGI GGATG 9 cut(s) 625, 694, 1069, 1100, 1210, 1244, 1798, 2168, 2950
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 8 cut(s) 183, 1585, 1730, 1823, 1826, 2283, 2467, 2468
BseMI GCAATG 1 cut(s) 2997
BseMII CTCAG 5 cut(s) 110, 691, 884, 2273, 2395
BseRI GAGGAG 1 cut(s) 864
BseXI GCAGC 9 cut(s) 445, 461, 899, 1099, 1102, 2059, 2343, 2937, 3139
Bsh1236I CGCG 1 cut(s) 1075
BshFI GGCC 7 cut(s) 87, 995, 1185, 1612, 2476, 3048, 3245
BshNI GGYRCC 1 cut(s) 428
BshVI ATCGAT 1 cut(s) 249
BsiHKAI GWGCWC 3 cut(s) 808, 1090, 3312
BsiHKCI CYCGRG 3 cut(s) 18, 990, 3323
BsiSI CCGG 3 cut(s) 658, 1653, 1724
BslFI GGGAC 3 cut(s) 560, 2674, 2770
BslI CCNNNNNNNGG 8 cut(s) 183, 1585, 1730, 1823, 1826, 2283, 2467, 2468
BsmAI GTCTC 4 cut(s) 324, 910, 1438, 2497
BsmFI GGGAC 3 cut(s) 560, 2674, 2770
BsnI GGCC 7 cut(s) 87, 995, 1185, 1612, 2476, 3048, 3245
BsoBI CYCGRG 3 cut(s) 18, 990, 3323
Bsp1286I GDGCHC 3 cut(s) 808, 1090, 3312
Bsp13I TCCGGA 1 cut(s) 1723
Bsp1407I TGTACA 2 cut(s) 220, 498
Bsp1720I GCTNAGC 1 cut(s) 96
BspACI CCGC 8 cut(s) 93, 327, 464, 814, 1182, 1544, 1790, 2151
BspANI GGCC 7 cut(s) 87, 995, 1185, 1612, 2476, 3048, 3245
BspCNI CTCAG 5 cut(s) 109, 690, 883, 2274, 2394
BspDI ATCGAT 1 cut(s) 249
BspEI TCCGGA 1 cut(s) 1723
BspFNI CGCG 1 cut(s) 1075
BspHI TCATGA 1 cut(s) 2866
BspLI GGNNCC 7 cut(s) 303, 430, 861, 1590, 1657, 2007, 2758
BspMAI CTGCAG 1 cut(s) 2049
BspPI GGATC 6 cut(s) 854, 867, 927, 1583, 1596, 2049
BspQI GCTCTTC 1 cut(s) 813
BspT107I GGYRCC 1 cut(s) 428
BspTI CTTAAG 2 cut(s) 784, 2051
BsrBI CCGCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 2997
BsrGI TGTACA 2 cut(s) 220, 498
BssECI CCNNGG 8 cut(s) 24, 453, 794, 847, 1187, 2082, 2146, 3076
BssNAI GTATAC 1 cut(s) 33
BssNI GRCGYC 1 cut(s) 1629
BssSI CACGAG 1 cut(s) 1716
BssT1I CCWWGG 3 cut(s) 24, 2146, 3076
Bst1107I GTATAC 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 1716
Bst2UI CCWGG 9 cut(s) 454, 795, 1371, 1580, 1614, 1824, 2084, 2436, 3050
Bst4CI ACNGT 9 cut(s) 437, 709, 731, 1279, 1975, 2249, 2408, 2734, 2804
Bst6I CTCTTC 5 cut(s) 813, 1236, 1410, 2572, 2577
BstACI GRCGYC 1 cut(s) 1629
BstAFI CTTAAG 2 cut(s) 784, 2051
BstAUI TGTACA 2 cut(s) 220, 498
BstBAI YACGTR 3 cut(s) 1871, 3020, 3188
BstC8I GCNNGC 6 cut(s) 468, 804, 1597, 1633, 3118, 3332
BstDEI CTNAG 8 cut(s) 96, 161, 677, 870, 1032, 2254, 2282, 2381
BstDSI CCRYGG 1 cut(s) 1187
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 9 cut(s) 625, 694, 1069, 1100, 1210, 1244, 1798, 2168, 2950
BstFNI CGCG 1 cut(s) 1075
BstH2I RGCGCY 1 cut(s) 2157
BstHHI GCGC 2 cut(s) 1077, 2156
BstMAI GTCTC 4 cut(s) 324, 910, 1438, 2497
BstNI CCWGG 9 cut(s) 454, 795, 1371, 1580, 1614, 1824, 2084, 2436, 3050
BstNSI RCATGY 3 cut(s) 137, 2092, 2372
BstSFI CTRYAG 3 cut(s) 1767, 2045, 2726
BstUI CGCG 1 cut(s) 1075
BstV1I GCAGC 9 cut(s) 445, 461, 899, 1099, 1102, 2059, 2343, 2937, 3139
BstV2I GAAGAC 4 cut(s) 1128, 1488, 2317, 2859
BstX2I RGATCY 4 cut(s) 859, 1588, 2041, 2116
BstXI CCANNNNNNTGG 1 cut(s) 2442
BstYI RGATCY 4 cut(s) 859, 1588, 2041, 2116
BstZ17I GTATAC 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 249
BsuI GTATCC 1 cut(s) 2700
BsuRI GGCC 7 cut(s) 87, 995, 1185, 1612, 2476, 3048, 3245
BsuTUI ATCGAT 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 1187
BtgZI GCGATG 1 cut(s) 2466
BtrI CACGTC 1 cut(s) 1762
BtsCI GGATG 9 cut(s) 625, 694, 1069, 1100, 1210, 1244, 1798, 2168, 2950
BtsI GCAGTG 1 cut(s) 171
Cac8I GCNNGC 6 cut(s) 468, 804, 1597, 1633, 3118, 3332
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 1 cut(s) 2866
CfoI GCGC 2 cut(s) 1077, 2156
Cfr13I GGNCC 6 cut(s) 302, 993, 1184, 1721, 1903, 2757
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 2 cut(s) 1637, 2996
Csp6I GTAC 9 cut(s) 90, 221, 499, 832, 1208, 2079, 2610, 2634, 3023
CspCI CAANNNNNGTGG 2 cut(s) 2628, 2663
CviQI GTAC 9 cut(s) 90, 221, 499, 832, 1208, 2079, 2610, 2634, 3023
DdeI CTNAG 8 cut(s) 96, 161, 677, 870, 1032, 2254, 2282, 2381
DrdI GACNNNNNNGTC 1 cut(s) 2489
DriI GACNNNNNGTC 1 cut(s) 300
DseDI GACNNNNNNGTC 1 cut(s) 2489
EaeI YGGCCR 2 cut(s) 1610, 3046
Eam1104I CTCTTC 5 cut(s) 813, 1236, 1410, 2572, 2577
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 5 cut(s) 813, 1236, 1410, 2572, 2577
Ecl136II GAGCTC 1 cut(s) 3310
Eco130I CCWWGG 3 cut(s) 24, 2146, 3076
Eco24I GRGCYC 1 cut(s) 3312
Eco47I GGWCC 4 cut(s) 302, 1721, 1903, 2757
Eco53kI GAGCTC 1 cut(s) 3310
Eco72I CACGTG 1 cut(s) 1871
Eco88I CYCGRG 3 cut(s) 18, 990, 3323
EcoICRI GAGCTC 1 cut(s) 3310
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoRI GAATTC 1 cut(s) 950
EcoRII CCWGG 9 cut(s) 452, 793, 1369, 1578, 1612, 1822, 2082, 2434, 3048
EcoT14I CCWWGG 3 cut(s) 24, 2146, 3076
EcoT22I ATGCAT 1 cut(s) 2814
EcoT38I GRGCYC 1 cut(s) 3312
ErhI CCWWGG 3 cut(s) 24, 2146, 3076
FalI AAGNNNNNCTT 6 cut(s) 1051, 1083, 1321, 1353, 3130, 3162
FaqI GGGAC 3 cut(s) 560, 2674, 2770
FblI GTMKAC 4 cut(s) 32, 2273, 2725, 2780
FokI GGATG 9 cut(s) 612, 701, 1076, 1087, 1217, 1231, 1805, 2155, 2957
FriOI GRGCYC 1 cut(s) 3312
FspBI CTAG 3 cut(s) 2010, 2076, 3260
GlaI GCGC 2 cut(s) 1076, 2155
GsuI CTGGAG 4 cut(s) 441, 1601, 2007, 2418
HaeII RGCGCY 1 cut(s) 2157
HaeIII GGCC 7 cut(s) 87, 995, 1185, 1612, 2476, 3048, 3245
HapII CCGG 3 cut(s) 658, 1653, 1724
HgaI GACGC 2 cut(s) 1637, 2996
HhaI GCGC 2 cut(s) 1077, 2156
Hin1I GRCGYC 1 cut(s) 1629
Hin6I GCGC 2 cut(s) 1075, 2154
HinP1I GCGC 2 cut(s) 1075, 2154
HincII GTYRAC 2 cut(s) 1399, 2305
HindII GTYRAC 2 cut(s) 1399, 2305
HindIII AAGCTT 4 cut(s) 77, 600, 1320, 3029
HpaI GTTAAC 1 cut(s) 1399
HpaII CCGG 3 cut(s) 658, 1653, 1724
HphI GGTGA 7 cut(s) 134, 1385, 1405, 1542, 1939, 2833, 3135
Hpy99I CGWCG 1 cut(s) 2219
HpyCH4III ACNGT 9 cut(s) 437, 709, 731, 1279, 1975, 2249, 2408, 2734, 2804
HpyCH4IV ACGT 7 cut(s) 895, 1761, 1870, 2217, 2646, 3019, 3187
HpyF3I CTNAG 8 cut(s) 96, 161, 677, 870, 1032, 2254, 2282, 2381
HpySE526I ACGT 7 cut(s) 895, 1761, 1870, 2217, 2646, 3019, 3187
Hsp92I GRCGYC 1 cut(s) 1629
HspAI GCGC 2 cut(s) 1075, 2154
Kpn2I TCCGGA 1 cut(s) 1723
KspAI GTTAAC 1 cut(s) 1399
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 4 cut(s) 466, 1511, 1655, 1882
Lsp1109I GCAGC 9 cut(s) 445, 461, 899, 1099, 1102, 2059, 2343, 2937, 3139
LweI GCATC 9 cut(s) 274, 479, 934, 960, 1054, 2424, 2668, 2855, 3164
MaeI CTAG 3 cut(s) 2010, 2076, 3260
MaeII ACGT 7 cut(s) 895, 1761, 1870, 2217, 2646, 3019, 3187
MaeIII GTNAC 7 cut(s) 166, 292, 485, 1548, 2131, 2249, 2935
MbiI CCGCTC 1 cut(s) 95
MfeI CAATTG 3 cut(s) 609, 1473, 1944
MflI RGATCY 4 cut(s) 859, 1588, 2041, 2116
MhlI GDGCHC 3 cut(s) 808, 1090, 3312
MlsI TGGCCA 2 cut(s) 1612, 3048
MluNI TGGCCA 2 cut(s) 1612, 3048
MlyI GAGTC 6 cut(s) 14, 1556, 1720, 2072, 2693, 3049
MmeI TCCRAC 7 cut(s) 347, 497, 979, 1120, 1489, 1977, 2196
Mox20I TGGCCA 2 cut(s) 1612, 3048
Mph1103I ATGCAT 1 cut(s) 2814
MroI TCCGGA 1 cut(s) 1723
MroXI GAANNNNTTC 1 cut(s) 1336
MscI TGGCCA 2 cut(s) 1612, 3048
MslI CAYNNNNRTG 2 cut(s) 239, 2229
Msp20I TGGCCA 2 cut(s) 1612, 3048
MspA1I CMGCKG 3 cut(s) 466, 890, 1182
MspCI CTTAAG 2 cut(s) 784, 2051
MspI CCGG 3 cut(s) 658, 1653, 1724
MunI CAATTG 3 cut(s) 609, 1473, 1944
MvaI CCWGG 9 cut(s) 454, 795, 1371, 1580, 1614, 1824, 2084, 2436, 3050
MvnI CGCG 1 cut(s) 1075
NciI CCSGG 1 cut(s) 658
NlaIV GGNNCC 7 cut(s) 303, 430, 861, 1590, 1657, 2007, 2758
NmuCI GTSAC 3 cut(s) 292, 1548, 2249
NsiI ATGCAT 1 cut(s) 2814
NspI RCATGY 3 cut(s) 137, 2092, 2372
PaeR7I CTCGAG 2 cut(s) 18, 3323
PagI TCATGA 1 cut(s) 2866
PciI ACATGT 1 cut(s) 2368
PciSI GCTCTTC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 1336
PfoI TCCNGGA 1 cut(s) 2434
PleI GAGTC 6 cut(s) 13, 1555, 1719, 2071, 2693, 3049
PmaCI CACGTG 1 cut(s) 1871
PmlI CACGTG 1 cut(s) 1871
PpsI GAGTC 6 cut(s) 13, 1555, 1719, 2071, 2693, 3049
Ppu21I YACGTR 3 cut(s) 1871, 3020, 3188
PscI ACATGT 1 cut(s) 2368
Psp124BI GAGCTC 1 cut(s) 3312
Psp6I CCWGG 9 cut(s) 452, 793, 1369, 1578, 1612, 1822, 2082, 2434, 3048
PspCI CACGTG 1 cut(s) 1871
PspGI CCWGG 9 cut(s) 452, 793, 1369, 1578, 1612, 1822, 2082, 2434, 3048
PspN4I GGNNCC 7 cut(s) 303, 430, 861, 1590, 1657, 2007, 2758
PspPI GGNCC 6 cut(s) 302, 993, 1184, 1721, 1903, 2757
PspXI VCTCGAGB 1 cut(s) 18
PstI CTGCAG 1 cut(s) 2049
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 4 cut(s) 859, 1588, 2041, 2116
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 9 cut(s) 91, 222, 500, 833, 1209, 2080, 2611, 2635, 3024
RsaNI GTAC 9 cut(s) 90, 221, 499, 832, 1208, 2079, 2610, 2634, 3023
RseI CAYNNNNRTG 2 cut(s) 239, 2229
SacI GAGCTC 1 cut(s) 3312
SapI GCTCTTC 1 cut(s) 813
Sau96I GGNCC 6 cut(s) 302, 993, 1184, 1721, 1903, 2757
SchI GAGTC 6 cut(s) 14, 1556, 1720, 2072, 2693, 3049
SduI GDGCHC 3 cut(s) 808, 1090, 3312
SfaNI GCATC 9 cut(s) 274, 479, 934, 960, 1054, 2424, 2668, 2855, 3164
SfcI CTRYAG 3 cut(s) 1767, 2045, 2726
Sfr274I CTCGAG 2 cut(s) 18, 3323
SinI GGWCC 4 cut(s) 302, 1721, 1903, 2757
SlaI CTCGAG 2 cut(s) 18, 3323
SmiMI CAYNNNNRTG 2 cut(s) 239, 2229
SmlI CTYRAG 7 cut(s) 18, 38, 784, 2051, 2060, 3311, 3323
SmoI CTYRAG 7 cut(s) 18, 38, 784, 2051, 2060, 3311, 3323
SpeI ACTAGT 1 cut(s) 2075
SsiI CCGC 8 cut(s) 93, 327, 464, 814, 1182, 1544, 1790, 2151
SspI AATATT 2 cut(s) 2191, 2839
SspMI CTAG 3 cut(s) 2010, 2076, 3260
SstI GAGCTC 1 cut(s) 3312
StyI CCWWGG 3 cut(s) 24, 2146, 3076
TaaI ACNGT 9 cut(s) 437, 709, 731, 1279, 1975, 2249, 2408, 2734, 2804
TaiI ACGT 7 cut(s) 898, 1764, 1873, 2220, 2649, 3022, 3190
TaqI TCGA 7 cut(s) 8, 19, 249, 2718, 3198, 3219, 3324
TaqII GACCGA 1 cut(s) 2774
TatI WGTACW 5 cut(s) 220, 498, 831, 2633, 3022
TauI GCSGC 2 cut(s) 1185, 2154
TseFI GTSAC 3 cut(s) 292, 1548, 2249
TseI GCWGC 9 cut(s) 449, 458, 887, 1112, 1115, 2047, 2331, 2950, 3152
Tsp45I GTSAC 3 cut(s) 292, 1548, 2249
TspDTI ATGAA 6 cut(s) 1121, 1295, 1511, 2241, 2580, 2855
TspGWI ACGGA 6 cut(s) 195, 898, 1023, 1480, 2322, 2597
Vha464I CTTAAG 2 cut(s) 784, 2051
VpaK11BI GGWCC 4 cut(s) 302, 1721, 1903, 2757
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 6 cut(s) 950, 1332, 2553, 2602, 2903, 3177
XceI RCATGY 3 cut(s) 137, 2092, 2372
XhoI CTCGAG 2 cut(s) 18, 3323
XmiI GTMKAC 4 cut(s) 32, 2273, 2725, 2780
XmnI GAANNNNTTC 1 cut(s) 1336
XspI CTAG 3 cut(s) 2010, 2076, 3260
Zsp2I ATGCAT 1 cut(s) 2814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.