MD11G1227200.v1.1

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007959
Physical Location & Seq
Reverse (-)
33078416 .. 33088348
9933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1227200.v1.1.491

Sequence Viewer

Length: 3651 bp
ATGGAGTCGATATTGGCTCGGGCGCTGGAGTACACTCTCAAGTACTGGTTGAAATCCTTCTCCAGAGACCAGTTCAAGTTGCAGGGACGGACAGTTCAGCTTTCTAATTTGGATATCAATGGCGATGCTTTGCACGCGAGCATGGGATTGCCACCGGCACTGAATGTAGCCACGGCCAAAGTTGGAAAATTGGAGATAGTGCTCCCATCTGTGAGTAATGTGCAATTAGAGCCGATTGTTGTGCAAATTGATAGGCTTGATTTGGTTTTGGAGGAGAAGTCAGATTTGGATGAGAGGAGCCCAAGAAGTTCCCCATCATCCTCTAGCTCAGCAAAGGGTAGCGGTTATGGATTTGCTGATAAGATTGCTGATGGAATGACTTTGGAGATTCTCACAGTTAATCTTCTACTTGAGACTCGTGGGGGTGGTCGAAGCCAAGGAGGCGCTAGTTGGGCATCACCTCTGGCCTCTATAACTATACACAACCTTTTGCTGTATACTACCAATGAAAATTGGCAGGTTGTAAATCTTAAGGAGGCACGGGAATTCTCCAGCGACAAAAATTTCATATATTTGTTCAAAAAACTTGAGTGGGAATCTCTGTCTATTGATCTCCTTCCCCACCCTGATATGTTCATGGATGCTGACATAGCACGTACTGAAGATGGAAGAAACCAGAGAGACGATGATGGTGCAAAGCGAGTTTTCTTTGGTGGGGAGCGCTTTATAGAAGGAATTTCAGGACAAGCTTATATCACAGTGCAAAGGACCGAATTAAACAGTCCACTTGGACTTGAGGTTCAGTTACATATCACAGAGGCCATTTGTCCTGCTATAAGTGAACCAGGACTACGTGCTCTTCTCCGCTTTATGACAGGATTGTATGTCTGTCTGAATAGAGGAGATGTTGATTCAAATACTCAGCAGCGATCCACAGAAGCAGCAGGACGTTCTATAGTCTCAATTGTTGTGGACCACATATTTCTCTGCATTAAAGATGCTGAGTTCAAGCTCGAACTTTTAATGCAATCACTCTTCTTCTCACGGGCCAGTGTTTCAGATGGAGAAATTGACAACAACTTGTCGAGGGTTATGATTGGTGGACTGTTTTTAAGGGATACCTATTCACGGCCTCCATGCACTTTGGTTCAACCATCAATGCATGCAGTTTCTGAAGAACCTCTGCATGTTCCTGACTTCGGTAAGAATTTTTGCCCTCCCATATATCCTTTGGGAGATCAGGAGTGGCAATTCATTAAAGGTGTCCCTTTTTTGTGCCTCCACTCTCTTCAGATCAAGCCATCCCCAGTCCCTCCATCTTTTGCTTCACAAACAGTAATTAATTGTCAACCCTTAATGATTGATCTCCAAGAAGGATCCTGTTTGAGGATCGCTTCCTTCCTGGCTGATGGAATTGTGGTCAATCCTGGTGCTGTTGTACCAGATTCCTCAGTAAATTCCTTGATTTTCACCCTCAAGGAGTTAGATGTGACTGTTCCTTTGGACATAGATAAGTTGTGTGACACTGCTAACAACAGGGACAATATCTACCAGAGTGCCTTTTCTGGAGCAAGGCTTCATATCAAGAACTTGTTAATTTCAGAGTCACCATCACTAAAACTAAGGTTACTGAATCTGGAGAAGGACCCTGCTTGCTTTTGTCTTTGGGAAGGTCAACCAATTGATGCCAGCCAGAAGAAATGGAGTGCTAGAGCATCACACATTAGTTTGTCATTAGAAAAGTGCACTAAGTCAGCTGGCTTTCAGAGTTCTATTGATTGGAATTCAGGCATGTGGAGATGTGTTGAGCTGAAAGATGCTTGTGTTGAAGTAGCTATGGTAACTGCTGATGGGAGCCCATTAACAAATGTTCCTCCTCCCGGTGGTATTGTCAGGGTGGGGGTAGCTTGTCAAAACTATCTCTCCAACACATCTGTTGAACAGTTATTTTTTGTTTTGGATCTTTATTCGTACTTTGGCAGAGTTAGTGAAAAGATTGTTCTTGTTGGAAAAAATACTGGAAAAAAGAAGAAGAGGGATCATTCTGTTGAATTAAAGCTGATTGACAAGGTTCCCAATGATACAGCGGTAAGCTTGGCAGTCAAAAATCTCCAGATTAAATTTCTGGAGTCATCTTCCGTGAATATTGAAGGAATGCCTCTGGTTCAGTTTATTGGTGATGATCTCTTCATAAAAGTTACTCACAGAACTCTTGGTGGTGCAATTGCTGTTTCATCCACTATTCATTGGGATAGTGTTGAGGTGGACTGTGTAGACACTGAGGGAAACTTGGGACATGAGAACGGCTCAGGTTTAACTTACATTGAAAATGGTCTTTCAACGAGTGGAAATGGGTACCCTCAGCTAAGACCTGTATTTTGGATACATAACCAAACAAAACATCAATCAAATGGCAAAGCTTTTGTGGATCCATTTCTGGACATAAGTATGGTACACGTAATTCCTTTAAATGAACGTGATGCAGAGTGTCATAGTCTGAATGTGTGTGCGTGTATCTCTGGCATACGCCTAGGTGGGGGAATGAACTATGCTGAATCCTTGCTGCATCGCTTTGGAGTACTTGGCCCTGATGGTGGTCCTGGAAAGGGGCTCTCTAAGGAGTTAGAAAAATTGCAAGCTGGACCACTGTCGAAACTTTTCAAACCGTCGCATCTCATTATTGATCTGAAAGAGGATGGAAGTTCACGAGACGGGAAAGAAAGTGGGGTCTTGCACTTGGGAAAGCCAGATGATGTCGACGTGTCCATAGAGTTCAAAAATTGGTTATTTGCTCTTGAAGGTGAACGGGAGATTGCAGAAAGATGGTGGTTTGATAATCATGAGGATGTGCAAAGAGAAGAGAGGTGTTGGCACACAACGTTCCATAGTCTGCATGTAAAAGCAAAAAGTAGTCCAAAGCATAGGCTGAGTGGCAATGGAAAGTCATACAGGACCCAAAAATATCCTGTGGAATTGGTTACAGTTGGTGTGCAAGGCTTGCAGATGCTAAAGCCCCATGCCCAGAAGTCTAACAATGCTGCTATCTTACCTGCGAATGGGATCAAGGAAACTACACAGACATCTGGAGGTATAGATCTTGAAATTCGCATGGTGATACCAGAGGACCCAGTCGATCATGAAATGGTGGTATGGGCTGTGGAAAATGTGAAATTCTCTGTTAAGCAACCGATTGAGGCAGTTGTAACGAAGGATGAGTTGCAACATCTTACTTTTCTGTGCAAGTCGGAAGTTGAATCAATGGGTCGAGTCACTGCTGGAATTCTGAGGCTACTCAAGCTGGAGGGTTCCATTGGCGAGGCTGCAATGGAACAACTGAGCAACCTCAGAACAGAGGGCATTGACAAGATATTCTCCCCTGGAAGGCTCACCAGGGGTGGCAGCTTTTCCAGTACTGGGCTCTCTCAATCAAACTTGGTTAATGAAACCCCAAGTACAACCCCCACCTTGGAATCGACAGTGGCTTCACTCGAGGTGGCATTTACAGAATCCCAGGCCAAGTGCACTGCTCTTCTTGCCGACCTAGATAGTTCAGAACAAGCCGCACAGCATCTTGCAACTGTTAAACAACTCAATGAGAAACTTCAGAGTATGCAGAGTTTATTGACGCAATTACGGTCACAGATATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1217

Amino Acids

133.81

Weight (kDa)

5.58

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 19 - 204 2.8e-07 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 508, 3058
Acc65I GGTACC 1 cut(s) 2355
AccB1I GGYRCC 1 cut(s) 2355
AccI GTMKAC 3 cut(s) 497, 2274, 2757
AccII CGCG 1 cut(s) 137
AciI CCGC 4 cut(s) 342, 865, 2087, 3561
AclI AACGTT 1 cut(s) 2879
AclWI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1968, 2046, 2423, 2436, 3068
AcoI YGGCCR 1 cut(s) 174
AcuI CTGAAG 4 cut(s) 681, 1194, 1274, 3587
AfeI AGCGCT 1 cut(s) 722
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 2 cut(s) 2455, 2760
AjiI CACGTC 1 cut(s) 2761
AjnI CCWGG 7 cut(s) 844, 1401, 1426, 2599, 3376, 3389, 3510
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 783, 815
Alw21I GWGCWC 4 cut(s) 204, 859, 1748, 3524
Alw26I GTCTC 5 cut(s) 60, 407, 675, 964, 2703
Alw44I GTGCAC 2 cut(s) 1744, 3520
AlwI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1968, 2046, 2423, 2436, 3068
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 2 cut(s) 18, 3488
Aor51HI AGCGCT 1 cut(s) 722
AoxI GGCC 7 cut(s) 174, 465, 819, 1047, 1130, 2584, 3513
ApaLI GTGCAC 2 cut(s) 1744, 3520
ApeKI GCWGC 6 cut(s) 925, 941, 2563, 3038, 3320, 3399
ArsI GACNNNNNNTTYG 4 cut(s) 1483, 1515, 2361, 2393
AseI ATTAAT 1 cut(s) 1341
Asp700I GAANNNNTTC 2 cut(s) 56, 2657
Asp718I GGTACC 1 cut(s) 2355
AspA2I CCTAGG 1 cut(s) 2530
AspLEI GCGC 3 cut(s) 25, 446, 723
AspS9I GGNCC 9 cut(s) 768, 973, 1047, 1645, 2585, 2597, 2642, 2952, 3124
AsuC2I CCSGG 1 cut(s) 1881
AsuHPI GGTGA 7 cut(s) 450, 1462, 1599, 2189, 2813, 3124, 3379
AvaI CYCGRG 2 cut(s) 18, 3488
AvaII GGWCC 7 cut(s) 768, 973, 1645, 2597, 2642, 2952, 3124
AvrII CCTAGG 1 cut(s) 2530
BaeGI GKGCMC 2 cut(s) 1748, 3524
BaeI ACNNNNGTAYC 2 cut(s) 2073, 2106
BamHI GGATCC 2 cut(s) 1376, 2428
BanI GGYRCC 1 cut(s) 2355
BanII GRGCYC 4 cut(s) 302, 1859, 2613, 3420
BauI CACGAG 2 cut(s) 417, 2706
Bbv12I GWGCWC 4 cut(s) 204, 859, 1748, 3524
BbvCI CCTCAGC 1 cut(s) 2361
BbvI GCAGC 6 cut(s) 937, 953, 2550, 3025, 3307, 3411
BceAI ACGGC 3 cut(s) 189, 1145, 2320
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 674, 708
BciT130I CCWGG 7 cut(s) 846, 1403, 1428, 2601, 3378, 3391, 3512
BciVI GTATCC 2 cut(s) 1111, 2376
BcnI CCSGG 1 cut(s) 1881
BcoDI GTCTC 5 cut(s) 60, 407, 675, 964, 2703
BfaI CTAG 5 cut(s) 324, 447, 1710, 2531, 3542
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 3 cut(s) 26, 447, 724
BfrI CTTAAG 1 cut(s) 530
BfuAI ACCTGC 2 cut(s) 508, 3058
BfuI GTATCC 2 cut(s) 1111, 2376
BglI GCCNNNNNGGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 3094
BisI GCNGC 7 cut(s) 926, 942, 2564, 3039, 3321, 3400, 3561
BlnI CCTAGG 1 cut(s) 2530
BlpI GCTNAGC 1 cut(s) 328
BlsI GCNGC 7 cut(s) 927, 943, 2565, 3040, 3322, 3401, 3562
BmcAI AGTACT 3 cut(s) 44, 2580, 3412
Bme1390I CCNGG 8 cut(s) 846, 1403, 1428, 1881, 2601, 3378, 3391, 3512
Bme18I GGWCC 7 cut(s) 768, 973, 1645, 2597, 2642, 2952, 3124
BmeT110I CYCGRG 2 cut(s) 18, 3488
BmgBI CACGTC 1 cut(s) 2761
BmgT120I GGNCC 9 cut(s) 768, 973, 1047, 1645, 2585, 2597, 2642, 2952, 3124
BmrFI CCNGG 8 cut(s) 846, 1403, 1428, 1881, 2601, 3378, 3391, 3512
BmrI ACTGGG 3 cut(s) 1301, 3122, 3423
BmuI ACTGGG 3 cut(s) 1301, 3122, 3423
BoxI GACNNNNGTC 1 cut(s) 2647
BplI GAGNNNNNCTC 2 cut(s) 2291, 2323
BpmI CTGGAG 9 cut(s) 46, 47, 535, 1587, 1658, 2096, 2147, 3105, 3320
Bpu10I CCTNAGC 2 cut(s) 2308, 2361
Bpu1102I GCTNAGC 1 cut(s) 328
BpuEI CTTGAG 6 cut(s) 23, 431, 608, 815, 1460, 3278
BpuMI CCSGG 1 cut(s) 1881
BsaAI YACGTR 3 cut(s) 656, 854, 2458
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 7 cut(s) 171, 436, 2530, 3376, 3390, 3465, 3510
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 1907, 1937
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 8 cut(s) 50, 70, 1050, 1307, 2023, 3128, 3408, 3418
Bse3DI GCAATG 2 cut(s) 2941, 3330
BseBI CCWGG 7 cut(s) 846, 1403, 1428, 2601, 3378, 3391, 3512
BseDI CCNNGG 7 cut(s) 171, 436, 2530, 3376, 3390, 3465, 3510
BseGI GGATG 8 cut(s) 295, 317, 646, 1301, 2234, 2701, 2851, 3217
BseMI GCAATG 2 cut(s) 2941, 3330
BseNI ACTGG 8 cut(s) 50, 70, 1050, 1307, 2023, 3128, 3408, 3418
BseRI GAGGAG 4 cut(s) 287, 310, 915, 1866
BseSI GKGCMC 2 cut(s) 1748, 3524
BseXI GCAGC 6 cut(s) 937, 953, 2550, 3025, 3307, 3411
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 7 cut(s) 176, 467, 821, 1049, 1132, 2586, 3515
BshNI GGYRCC 1 cut(s) 2355
BsiHKAI GWGCWC 4 cut(s) 204, 859, 1748, 3524
BsiHKCI CYCGRG 2 cut(s) 18, 3488
BsiSI CCGG 2 cut(s) 155, 1881
BslFI GGGAC 5 cut(s) 99, 1250, 1295, 1553, 2307
BsmAI GTCTC 5 cut(s) 60, 407, 675, 964, 2703
BsmBI CGTCTC 2 cut(s) 675, 2703
BsmFI GGGAC 5 cut(s) 99, 1250, 1295, 1553, 2307
BsmI GAATGC 1 cut(s) 2160
BsnI GGCC 7 cut(s) 176, 467, 821, 1049, 1132, 2586, 3515
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 2 cut(s) 18, 3488
Bsp1286I GDGCHC 8 cut(s) 204, 302, 859, 1748, 1859, 2613, 3420, 3524
Bsp1720I GCTNAGC 1 cut(s) 328
BspACI CCGC 4 cut(s) 342, 865, 2087, 3561
BspANI GGCC 7 cut(s) 176, 467, 821, 1049, 1132, 2586, 3515
BspFNI CGCG 1 cut(s) 137
BspHI TCATGA 2 cut(s) 2839, 3136
BspMI ACCTGC 2 cut(s) 508, 3058
BspPI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1968, 2046, 2423, 2436, 3068
BspQI GCTCTTC 2 cut(s) 864, 3534
BspT107I GGYRCC 1 cut(s) 2355
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 2 cut(s) 2941, 3330
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 8 cut(s) 50, 70, 1050, 1307, 2023, 3128, 3408, 3418
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 7 cut(s) 171, 436, 2530, 3376, 3390, 3465, 3510
BssNAI GTATAC 1 cut(s) 498
BssSI CACGAG 2 cut(s) 417, 2706
BssT1I CCWWGG 3 cut(s) 436, 2530, 3465
Bst1107I GTATAC 1 cut(s) 498
Bst2BI CACGAG 2 cut(s) 417, 2706
Bst2UI CCWGG 7 cut(s) 846, 1403, 1428, 2601, 3378, 3391, 3512
Bst6I CTCTTC 7 cut(s) 864, 1040, 1293, 2027, 2192, 2853, 3534
BstAFI CTTAAG 1 cut(s) 530
BstAPI GCANNNNNTGC 1 cut(s) 2998
BstBAI YACGTR 3 cut(s) 656, 854, 2458
BstC8I GCNNGC 8 cut(s) 135, 139, 1164, 1654, 1690, 1759, 2637, 2999
BstDSI CCRYGG 1 cut(s) 171
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 8 cut(s) 295, 317, 646, 1301, 2234, 2701, 2851, 3217
BstFNI CGCG 1 cut(s) 137
BstH2I RGCGCY 3 cut(s) 26, 447, 724
BstHHI GCGC 3 cut(s) 25, 446, 723
BstMAI GTCTC 5 cut(s) 60, 407, 675, 964, 2703
BstMWI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2998, 3295, 3533
BstNI CCWGG 7 cut(s) 846, 1403, 1428, 2601, 3378, 3391, 3512
BstNSI RCATGY 4 cut(s) 1166, 1190, 1795, 2897
BstPAI GACNNNNGTC 1 cut(s) 2647
BstSCI CCNGG 8 cut(s) 844, 1401, 1426, 1879, 2599, 3376, 3389, 3510
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 2 cut(s) 1748, 3524
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 6 cut(s) 937, 953, 2550, 3025, 3307, 3411
BstX2I RGATCY 4 cut(s) 1376, 1960, 2428, 3094
BstYI RGATCY 4 cut(s) 1376, 1960, 2428, 3094
BstZ17I GTATAC 1 cut(s) 498
BsuI GTATCC 2 cut(s) 1111, 2376
BsuRI GGCC 7 cut(s) 176, 467, 821, 1049, 1132, 2586, 3515
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 2 cut(s) 138, 2552
BtrI CACGTC 1 cut(s) 2761
BtsCI GGATG 8 cut(s) 295, 317, 646, 1301, 2234, 2701, 2851, 3217
BtsI GCAGTG 3 cut(s) 1524, 3270, 3522
BtsIMutI CAGTG 9 cut(s) 158, 765, 1057, 1524, 2277, 2645, 3270, 3483, 3522
BveI ACCTGC 2 cut(s) 508, 3058
Cac8I GCNNGC 8 cut(s) 135, 139, 1164, 1654, 1690, 1759, 2637, 2999
CaiI CAGNNNCTG 1 cut(s) 1172
CciI TCATGA 2 cut(s) 2839, 3136
CfoI GCGC 3 cut(s) 25, 446, 723
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 9 cut(s) 768, 973, 1047, 1645, 2585, 2597, 2642, 2952, 3124
CseI GACGC 1 cut(s) 3634
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
DraI TTTAAA 1 cut(s) 2469
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 7 cut(s) 864, 1040, 1293, 2027, 2192, 2853, 3534
EarI CTCTTC 7 cut(s) 864, 1040, 1293, 2027, 2192, 2853, 3534
Eco130I CCWWGG 3 cut(s) 436, 2530, 3465
Eco24I GRGCYC 4 cut(s) 302, 1859, 2613, 3420
Eco31I GGTCTC 1 cut(s) 60
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 7 cut(s) 768, 973, 1645, 2597, 2642, 2952, 3124
Eco47III AGCGCT 1 cut(s) 722
Eco57I CTGAAG 4 cut(s) 681, 1194, 1274, 3587
Eco88I CYCGRG 2 cut(s) 18, 3488
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoO109I RGGNCCY 3 cut(s) 1645, 2952, 3124
EcoRI GAATTC 3 cut(s) 545, 1783, 3279
EcoRII CCWGG 7 cut(s) 844, 1401, 1426, 2599, 3376, 3389, 3510
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 3 cut(s) 436, 2530, 3465
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 4 cut(s) 302, 1859, 2613, 3420
ErhI CCWWGG 3 cut(s) 436, 2530, 3465
Esp3I CGTCTC 2 cut(s) 675, 2703
FaqI GGGAC 5 cut(s) 99, 1250, 1295, 1553, 2307
FblI GTMKAC 3 cut(s) 497, 2274, 2757
Fnu4HI GCNGC 7 cut(s) 926, 942, 2564, 3039, 3321, 3400, 3561
FokI GGATG 8 cut(s) 302, 304, 653, 1288, 2221, 2708, 2858, 3224
FriOI GRGCYC 4 cut(s) 302, 1859, 2613, 3420
Fsp4HI GCNGC 7 cut(s) 926, 942, 2564, 3039, 3321, 3400, 3561
FspBI CTAG 5 cut(s) 324, 447, 1710, 2531, 3542
GlaI GCGC 3 cut(s) 24, 445, 722
GluI GCNGC 7 cut(s) 926, 942, 2564, 3039, 3321, 3400, 3561
GsuI CTGGAG 9 cut(s) 46, 47, 535, 1587, 1658, 2096, 2147, 3105, 3320
HaeII RGCGCY 3 cut(s) 26, 447, 724
HaeIII GGCC 7 cut(s) 176, 467, 821, 1049, 1132, 2586, 3515
HapII CCGG 2 cut(s) 155, 1881
HgaI GACGC 1 cut(s) 3634
HhaI GCGC 3 cut(s) 25, 446, 723
Hin6I GCGC 3 cut(s) 23, 444, 721
HinP1I GCGC 3 cut(s) 23, 444, 721
HincII GTYRAC 3 cut(s) 1349, 1676, 2758
HindII GTYRAC 3 cut(s) 1349, 1676, 2758
HindIII AAGCTT 3 cut(s) 747, 2092, 2418
HpaII CCGG 2 cut(s) 155, 1881
HphI GGTGA 7 cut(s) 450, 1462, 1599, 2189, 2813, 3124, 3379
Hpy99I CGWCG 2 cut(s) 2671, 2762
HpyCH4IV ACGT 7 cut(s) 655, 853, 949, 2457, 2476, 2760, 2879
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2998, 3295, 3533
HpySE526I ACGT 7 cut(s) 655, 853, 949, 2457, 2476, 2760, 2879
HspAI GCGC 3 cut(s) 23, 444, 721
KpnI GGTACC 1 cut(s) 2359
LguI GCTCTTC 2 cut(s) 864, 3534
LmnI GCTCC 5 cut(s) 207, 297, 718, 1568, 1854
Lsp1109I GCAGC 6 cut(s) 937, 953, 2550, 3025, 3307, 3411
MaeI CTAG 5 cut(s) 324, 447, 1710, 2531, 3542
MaeII ACGT 7 cut(s) 655, 853, 949, 2457, 2476, 2760, 2879
MfeI CAATTG 3 cut(s) 963, 1680, 2223
MflI RGATCY 4 cut(s) 1376, 1960, 2428, 3094
MhlI GDGCHC 8 cut(s) 204, 302, 859, 1748, 1859, 2613, 3420, 3524
MlyI GAGTC 5 cut(s) 14, 409, 1613, 2138, 3276
MmeI TCCRAC 4 cut(s) 163, 1950, 1987, 3225
Mph1103I ATGCAT 1 cut(s) 1164
MroXI GAANNNNTTC 2 cut(s) 56, 2657
MslI CAYNNNNRTG 3 cut(s) 2447, 2844, 3643
MspA1I CMGCKG 2 cut(s) 1757, 2087
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 2 cut(s) 155, 1881
MspR9I CCNGG 8 cut(s) 846, 1403, 1428, 1881, 2601, 3378, 3391, 3512
MunI CAATTG 3 cut(s) 963, 1680, 2223
Mva1269I GAATGC 1 cut(s) 2160
MvaI CCWGG 7 cut(s) 846, 1403, 1428, 2601, 3378, 3391, 3512
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 8 cut(s) 134, 229, 441, 452, 650, 2998, 3295, 3533
NciI CCSGG 1 cut(s) 1881
NmuCI GTSAC 5 cut(s) 1489, 1520, 1605, 3268, 3636
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 4 cut(s) 1166, 1190, 1795, 2897
PaeI GCATGC 1 cut(s) 1166
PaeR7I CTCGAG 1 cut(s) 3488
PagI TCATGA 2 cut(s) 2839, 3136
PciSI GCTCTTC 2 cut(s) 864, 3534
PctI GAATGC 1 cut(s) 2160
PdmI GAANNNNTTC 2 cut(s) 56, 2657
PfeI GAWTC 9 cut(s) 388, 596, 911, 1445, 1633, 2555, 3254, 3470, 3506
PflFI GACNNNGTC 1 cut(s) 3128
PfoI TCCNGGA 1 cut(s) 2599
PkrI GCNGC 7 cut(s) 927, 943, 2565, 3040, 3322, 3401, 3562
PleI GAGTC 5 cut(s) 13, 409, 1612, 2137, 3275
PpsI GAGTC 5 cut(s) 13, 409, 1612, 2137, 3275
Ppu21I YACGTR 3 cut(s) 656, 854, 2458
PpuMI RGGWCCY 3 cut(s) 1645, 2952, 3124
PshAI GACNNNNGTC 1 cut(s) 2647
PshBI ATTAAT 1 cut(s) 1341
Psp1406I AACGTT 1 cut(s) 2879
Psp5II RGGWCCY 3 cut(s) 1645, 2952, 3124
Psp6I CCWGG 7 cut(s) 844, 1401, 1426, 2599, 3376, 3389, 3510
PspGI CCWGG 7 cut(s) 844, 1401, 1426, 2599, 3376, 3389, 3510
PspPI GGNCC 9 cut(s) 768, 973, 1047, 1645, 2585, 2597, 2642, 2952, 3124
PspPPI RGGWCCY 3 cut(s) 1645, 2952, 3124
PspXI VCTCGAGB 1 cut(s) 3488
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 4 cut(s) 1376, 1960, 2428, 3094
PsyI GACNNNGTC 1 cut(s) 3128
PvuII CAGCTG 1 cut(s) 1757
RseI CAYNNNNRTG 3 cut(s) 2447, 2844, 3643
SalI GTCGAC 1 cut(s) 2756
SapI GCTCTTC 2 cut(s) 864, 3534
SatI GCNGC 7 cut(s) 926, 942, 2564, 3039, 3321, 3400, 3561
Sau96I GGNCC 9 cut(s) 768, 973, 1047, 1645, 2585, 2597, 2642, 2952, 3124
ScaI AGTACT 3 cut(s) 44, 2580, 3412
SchI GAGTC 5 cut(s) 14, 409, 1613, 2138, 3276
ScrFI CCNGG 8 cut(s) 846, 1403, 1428, 1881, 2601, 3378, 3391, 3512
SduI GDGCHC 8 cut(s) 204, 302, 859, 1748, 1859, 2613, 3420, 3524
SfcI CTRYAG 1 cut(s) 954
Sfr274I CTCGAG 1 cut(s) 3488
SinI GGWCC 7 cut(s) 768, 973, 1645, 2597, 2642, 2952, 3124
SlaI CTCGAG 1 cut(s) 3488
SmiMI CAYNNNNRTG 3 cut(s) 2447, 2844, 3643
SmlI CTYRAG 8 cut(s) 38, 410, 530, 587, 794, 1475, 3293, 3488
SmoI CTYRAG 8 cut(s) 38, 410, 530, 587, 794, 1475, 3293, 3488
SphI GCATGC 1 cut(s) 1166
SsiI CCGC 4 cut(s) 342, 865, 2087, 3561
SspI AATATT 1 cut(s) 2146
SspMI CTAG 5 cut(s) 324, 447, 1710, 2531, 3542
StyD4I CCNGG 8 cut(s) 844, 1401, 1426, 1879, 2599, 3376, 3389, 3510
StyI CCWWGG 3 cut(s) 436, 2530, 3465
TaiI ACGT 7 cut(s) 658, 856, 952, 2460, 2479, 2763, 2882
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 5 cut(s) 30, 42, 2578, 3410, 3452
TauI GCSGC 1 cut(s) 3563
TfiI GAWTC 9 cut(s) 388, 596, 911, 1445, 1633, 2555, 3254, 3470, 3506
TscAI CASTG 9 cut(s) 165, 765, 1057, 1531, 2284, 2652, 3277, 3483, 3529
TseFI GTSAC 5 cut(s) 1489, 1520, 1605, 3268, 3636
TseI GCWGC 6 cut(s) 925, 941, 2563, 3038, 3320, 3399
Tsp45I GTSAC 5 cut(s) 1489, 1520, 1605, 3268, 3636
TspGWI ACGGA 2 cut(s) 103, 2128
TspRI CASTG 9 cut(s) 165, 765, 1057, 1531, 2284, 2652, 3277, 3483, 3529
Tth111I GACNNNGTC 1 cut(s) 3128
Vha464I CTTAAG 1 cut(s) 530
VneI GTGCAC 2 cut(s) 1744, 3520
VpaK11BI GGWCC 7 cut(s) 768, 973, 1645, 2597, 2642, 2952, 3124
VspI ATTAAT 1 cut(s) 1341
XagI CCTNNNNNAGG 1 cut(s) 1384
XceI RCATGY 4 cut(s) 1166, 1190, 1795, 2897
XcmI CCANNNNNNNNNTGG 1 cut(s) 1228
XhoI CTCGAG 1 cut(s) 3488
XmaJI CCTAGG 1 cut(s) 2530
XmiI GTMKAC 3 cut(s) 497, 2274, 2757
XmnI GAANNNNTTC 2 cut(s) 56, 2657
XspI CTAG 5 cut(s) 324, 447, 1710, 2531, 3542
ZrmI AGTACT 3 cut(s) 44, 2580, 3412
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.