MD03G1211800.v1.1

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007959
Physical Location & Seq
Reverse (-)
29067257 .. 29079739
12483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1211800.v1.1.491

Sequence Viewer

Length: 3642 bp
ATGGAGTCGATATTGGCTCGGGCGCTGGAGTACACTCTCAAATATTGGTTGAAATCTTTCTCCAGAGACCAGTTCAAGTTGCAGGGACGGACGGTCCAGCTTTCTAATTTAGATATCAATGGCGATGCTTTGCATTCGAGCATGGGATTGCCGCCGGCACTGAACGTAGCCACGGCCAAAGTTGGAAAGTTGGAGATAGTGCTCCCATCTGTCAGTAATGTGCAAATAGAGCCGATTGTCGTGCAAATTGATAGGCTTGATTTGGTTCTGGAGGAGAAGTCAGATTTGGATGAGAGGAGTCCAAGGAGTTCCCCATCATCCTCTAGCTCAGCAATGGGCAGCGGTTATGGATTTGCTGATAAGATTGCAGATGGAATGACTTTGGAGATTCTCACAGTTAATCTTCTACTTGAGACTCGTGGGGGTGGTCGAAGCCATGAAGGCGCTAGTTGGGCATCACCTCTGGGCTCTATAACTATACGGAACCTTTTTCTGTATACTACCAATGAAAATTGGCAGGTTGTAAGTCTTAAGGAATCACGGGAATTCTCCAGTGACAAAAAGTTCATATATTTGTTCAAAAAACTTGAGTGGGAATCTTTGTCTATTGATCTCCTTCCCCACCCTGATATGTTCATGGATGCTAATATAGCACGTACTGAAGATGGAAGAAACCAGAGAGATGACGATGGTGCAAAGCGAGTTTTCTTTGGTGGGGAGCGCTTTATAGAAGGAATTTCAGGACAAGCTTATATCACAGTGCAAAGGACTGAATTAAACAGTCCACTTGGACTTGAGGTTCAGTTACATATCACAGAGGCCATTTGTCCTGCTATAAGTGAACCAGGACTACGTGCTCTCCTTCGCTTTATGACAGGATTGTATGTATGTCTGAATAGAGGAGATGTTGATTCAAATACTCAGCAGCGATCCACACAAGCAGCAGGACGTTCTATTGTCTCAATTGTTGTGGACCACATTTTTCTTTGCATTAAAGATGCTGAGTTCAAGCTTGAACTTTTGATGCAATCACTCTTCTTCTCACGGGCCAGTGTTTCAGATGGAGAAATTGACAACAACTTGTCGAGGGTTATGATTGGTGGACTGTTTTTAAGGGATACCTTTTCACGACCTCCCTGCACTTTGGTTCAACCATCAATGCATGCAGTTTCTGAAAAACCTCTCCATGTTCCTGACTTTGGTAAGAACTTTTGCCCTCCCATATATCCTTTGGGAGATCAGGAGTGGCAGCTAATTAAAGGTGTCCCTTTTTTGTGCCTTCACTCTCTTCAGATCAAGCCATCCCCAATCCCTCCATCTTTTGCTTCACAAACAGTAATTAATTGTCAACCCCTAATGATTGATCTCCAAGAAGGATCCTGTTTGAGGATCGCTTCCTTCCTGGCTGATGGAATTGTAGTCAATCCTGGTGCTGTTTTACCAGATTTCTCAGTCAATTCGTTGACTTTCACCCTGAAGGAATTAGATGTGACTGTTCCTTTGGACAGAGATAAATTGTGTGACCGTGCTAACAAGGACAGTATCTACCAGAGTACCTTTTCTGGAGCAAGGCTTCATATCGAGAACTTGTTATTTTCGGAGTCACCATCACTAAAACTAAGGTTACTGAATCTGGAGAAGGATCCTGCTTGTTTTTGTCTTTGGGAAGATCAACCAATTGATGCCAGCCAGAAGAAATGGAGTGCTAGAGCATCGCACATCAGTTTGTCACTAGAAAAATGCACCAAGTCTGCTGGCCTTCAGGGTTCTCTTGATGGGAATTCAGGCATGTGGAGATGTGTTGAGCTGAAAGATGCTTGTGTTGAAGTAGCTATGGTAACTGCTGATGGGAGCCCATTAACAAATGTTCCTCCCCCCCGTGGTATTGTCAGGGTGGGGGTAGCTTGTCAAAACTATCTCTCCAACACATCTGTTGAACAGTTATTTTTTGTTTTGGATCTTTATTCGTACTTTGGCAGAGTTAGTGAAAAGATTGTTCTCATTGGAAAAAATACTGGAAAAAAGAATAAGGATCATTCTATTGACTTAAAGCTGATTGACAAGGTTCCCAATGATACAGCGGTAAGCTTGGCAGTAAAAAATCTTCAGATTAAATTTCTGGAATCATCTTCCGTGAATATTGAAGGAATGCCTCTGGTTCAGTTTATTGGGGATGATCTCTTTATAAAAGTTACTCACAGAACTCTCGGTGGTGCAGTTGCTGTTTCATCCACTATACATTGGGATAGTGTTGAGGTGGACTGTGTAGACACTGAGGGAAACTTGGCACATGAGAATGGCTCAGTTTTAACTTCTGTTGAAAATGGTCTTTCAACTAGTGGAAATGGGTACCCTCAGCTAAGACCTGTATTTTGGATACATAACCAAATAAAACATCAATCAAATGGAAAAGCTTTTGTAAATCCATTTCTGGACGTAAGCATGGTACACGTAATTCCTTTAAATGAACGAGATGCAGAGTGTCATAGTCTGAATGTGTCGGCGTGTATCTCTGGCATACGCCTAAGTGGGGGAATGAACTATGCTGAATCCCTGCTGCATCGCTTTGGAATACTTGGCCCTGATGGTGGTCCAGGAAAGGGGCTCTCTAAGGAGTTAGAAAAATTACAAGCTGGACCACTGTCAAAACTTTTCAAACCGTTGCATCTCATTGTTGATGTGAAAGAGGATGGAAGTCCAGGAGATGGGAGAGAAAGTGGGGTCTTGCACTTGGGAAAGCCAGATGATGTAGACGTGTCCGTAGAGTTCAAAAATTGGTTATTTGCTCTTGAAGGTGAACGGGAGATTGCAGAAAGATGGTGGTTCGACAATCATGAGGATGTGCAAAGAGAAGAGAGGTGTTGGCACACAACGTTCCATAATCTGCATGTAAAAGCGAAAAGTGGTCCAAAGCATATGCTGAATGGTAATGGAAAGTCATACAGGACCCAAAAATATCCTGTGGAATTGGTTACAGTTGGTGTGGAAGGCTTGCAGATACTAAAGCCACATTCTCAGAAGTCTAACAATGTTGCTGTCTTACCTGCGAATGGGATCAAGGAAACTGCTGAGACATCTGCAGGTATAGATCTTGAATTTCGCATGGTGATACCAGAGGACCCGGTCGATCACAAAATGGTGGAATGGGCTGTGGAAAATGTGAAATTCTCTGTTAAGCAACCGATTGAGGCGGTTGTAACGAAGGATGAGTTGCAACATCTTACTTTTCTGTGCAAGTCGGAAGTTGAATCAATGGGTCGAATGACGGCTGGAATTCTGCGGTTACTCAAGCTGGAGGGGTCCATTGGTGAGGCTGCAATGGACCAACTAAGCAACCTTGGAACTGAGGGCATCGACAAGTTACTCTCACCAGCAAAGCTCAGCAGGGGCGGCAGCTTTTCCAGCACGGGGCTCTCTCAGTCAAATTTGATTATTGAAACCCCAAGTACAACCGCGACCTTGGAATCGACAGTGGCTTCACTTGAGGAGGCATTTACAGATTCACAGTCCAAGTGCACTGCTCTTCTTGCTGACCTAGGTAGTTCAGAGCCGTCTGCACAGCATCTTGCGACTGTAAAAGAACTCAATCAGAAACTTCAGAGTATGCAGAGTTTATTGACGCAGTTACGGTCTCACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1214

Amino Acids

133.37

Weight (kDa)

5.57

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 19 - 204 1.8e-06 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2186
Acc36I ACCTGC 3 cut(s) 508, 3052, 3071
Acc65I GGTACC 1 cut(s) 2349
AccB1I GGYRCC 1 cut(s) 2349
AccB7I CCANNNNNTGG 1 cut(s) 2705
AccI GTMKAC 3 cut(s) 497, 2268, 2751
AccII CGCG 1 cut(s) 3455
AciI CCGC 7 cut(s) 152, 342, 2081, 3191, 3280, 3390, 3453
AclI AACGTT 1 cut(s) 2873
AclWI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1637, 1650, 1965, 2040, 3062
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 8 cut(s) 545, 735, 1780, 2114, 3095, 3164, 3273, 3424
AcuI CTGAAG 6 cut(s) 681, 1274, 1496, 1745, 2090, 3581
AfaI GTAC 7 cut(s) 32, 658, 1555, 1970, 2351, 2448, 3448
AfeI AGCGCT 1 cut(s) 722
AfiI CCNNNNNNNGG 9 cut(s) 1199, 1386, 1880, 2530, 2588, 2600, 2705, 3050, 3408
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 2 cut(s) 2449, 2754
AhdI GACNNNNNGTC 1 cut(s) 92
AhlI ACTAGT 1 cut(s) 2336
AjiI CACGTC 1 cut(s) 2755
AjnI CCWGG 5 cut(s) 844, 1401, 1426, 2593, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AloI GAACNNNNNNTCC 2 cut(s) 783, 815
Alw21I GWGCWC 3 cut(s) 204, 859, 3518
Alw26I GTCTC 5 cut(s) 60, 407, 964, 3065, 3636
Alw44I GTGCAC 1 cut(s) 3514
AlwI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1637, 1650, 1965, 2040, 3062
AlwNI CAGNNNCTG 2 cut(s) 1172, 2222
Ama87I CYCGRG 1 cut(s) 18
Aor51HI AGCGCT 1 cut(s) 722
AoxI GGCC 5 cut(s) 174, 819, 1047, 1756, 2578
ApaLI GTGCAC 1 cut(s) 3514
ApeKI GCWGC 7 cut(s) 339, 925, 941, 1249, 2557, 3314, 3393
ApoI RAATTY 8 cut(s) 545, 735, 1780, 2114, 3095, 3164, 3273, 3424
ArsI GACNNNNNNTTYG 4 cut(s) 1483, 1515, 2355, 2387
AseI ATTAAT 1 cut(s) 1341
Asp700I GAANNNNTTC 1 cut(s) 56
Asp718I GGTACC 1 cut(s) 2349
AspA2I CCTAGG 1 cut(s) 3535
AspLEI GCGC 3 cut(s) 25, 446, 723
AsuC2I CCSGG 1 cut(s) 3122
AsuHPI GGTGA 7 cut(s) 450, 1462, 1596, 2807, 3118, 3320, 3360
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 9 cut(s) 94, 973, 2591, 2636, 2906, 2946, 3118, 3300, 3322
AvrII CCTAGG 1 cut(s) 3535
BaeGI GKGCMC 1 cut(s) 3518
BaeI ACNNNNGTAYC 4 cut(s) 1537, 1570, 2067, 2100
BamHI GGATCC 2 cut(s) 1376, 1642
BanI GGYRCC 1 cut(s) 2349
BanII GRGCYC 4 cut(s) 470, 1856, 2607, 3414
BarI GAAGNNNNNNTAC 2 cut(s) 2088, 2120
BauI CACGAG 1 cut(s) 417
Bbv12I GWGCWC 3 cut(s) 204, 859, 3518
BbvCI CCTCAGC 1 cut(s) 2355
BbvI GCAGC 7 cut(s) 351, 937, 953, 1261, 2544, 3301, 3405
BceAI ACGGC 3 cut(s) 189, 3282, 3535
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 1119, 1153
BciT130I CCWGG 5 cut(s) 846, 1403, 1428, 2595, 2700
BciVI GTATCC 2 cut(s) 1111, 2370
BcnI CCSGG 1 cut(s) 3122
BcoDI GTCTC 5 cut(s) 60, 407, 964, 3065, 3636
BcuI ACTAGT 1 cut(s) 2336
BfaI CTAG 6 cut(s) 324, 447, 1707, 1733, 2337, 3536
BfmI CTRYAG 1 cut(s) 3078
BfoI RGCGCY 3 cut(s) 26, 447, 724
BfrI CTTAAG 1 cut(s) 530
BfuAI ACCTGC 3 cut(s) 508, 3052, 3071
BfuI GTATCC 2 cut(s) 1111, 2370
BglI GCCNNNNNGGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 3088
BisI GCNGC 9 cut(s) 152, 340, 926, 942, 1250, 2558, 3315, 3391, 3394
BlnI CCTAGG 1 cut(s) 3535
BlpI GCTNAGC 2 cut(s) 328, 3380
BlsI GCNGC 9 cut(s) 153, 341, 927, 943, 1251, 2559, 3316, 3392, 3395
Bme1390I CCNGG 6 cut(s) 846, 1403, 1428, 2595, 2700, 3122
Bme18I GGWCC 9 cut(s) 94, 973, 2591, 2636, 2906, 2946, 3118, 3300, 3322
BmeRI GACNNNNNGTC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 1 cut(s) 2755
BmiI GGNNCC 9 cut(s) 485, 1378, 1644, 1853, 2067, 2351, 2948, 3120, 3301
BmrFI CCNGG 6 cut(s) 846, 1403, 1428, 2595, 2700, 3122
BoxI GACNNNNGTC 1 cut(s) 2641
BplI GAGNNNNNCTC 2 cut(s) 2285, 2317
BpmI CTGGAG 7 cut(s) 46, 47, 290, 535, 1584, 1655, 3314
Bpu10I CCTNAGC 1 cut(s) 2355
Bpu1102I GCTNAGC 2 cut(s) 328, 3380
BpuEI CTTGAG 5 cut(s) 431, 608, 815, 3272, 3503
BpuMI CCSGG 1 cut(s) 3122
BsaAI YACGTR 3 cut(s) 656, 854, 2452
BsaI GGTCTC 2 cut(s) 60, 3636
BsaJI CCNNGG 6 cut(s) 171, 302, 1879, 3337, 3459, 3535
BsaXI ACNNNNNCTCC 8 cut(s) 263, 293, 843, 873, 1904, 1934, 2701, 2731
Bsc4I CCNNNNNNNGG 9 cut(s) 1199, 1386, 1880, 2530, 2588, 2600, 2705, 3050, 3408
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 4 cut(s) 70, 552, 1050, 2020
Bse3DI GCAATG 2 cut(s) 339, 3324
BseBI CCWGG 5 cut(s) 846, 1403, 1428, 2595, 2700
BseDI CCNNGG 6 cut(s) 171, 302, 1879, 3337, 3459, 3535
BseGI GGATG 9 cut(s) 295, 317, 646, 1301, 2179, 2228, 2695, 2845, 3211
BseLI CCNNNNNNNGG 9 cut(s) 1199, 1386, 1880, 2530, 2588, 2600, 2705, 3050, 3408
BseMI GCAATG 2 cut(s) 339, 3324
BseNI ACTGG 4 cut(s) 70, 552, 1050, 2020
BseRI GAGGAG 4 cut(s) 287, 310, 915, 3500
BseSI GKGCMC 1 cut(s) 3518
BseXI GCAGC 7 cut(s) 351, 937, 953, 1261, 2544, 3301, 3405
BsgI GTGCAG 3 cut(s) 1123, 2235, 3540
Bsh1236I CGCG 1 cut(s) 3455
Bsh1285I CGRYCG 1 cut(s) 3126
BshFI GGCC 5 cut(s) 176, 821, 1049, 1758, 2580
BshNI GGYRCC 1 cut(s) 2349
BsiEI CGRYCG 1 cut(s) 3126
BsiHKAI GWGCWC 3 cut(s) 204, 859, 3518
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 2 cut(s) 155, 3122
BslFI GGGAC 2 cut(s) 99, 1250
BslI CCNNNNNNNGG 9 cut(s) 1199, 1386, 1880, 2530, 2588, 2600, 2705, 3050, 3408
BsmAI GTCTC 5 cut(s) 60, 407, 964, 3065, 3636
BsmFI GGGAC 2 cut(s) 99, 1250
BsmI GAATGC 2 cut(s) 133, 2154
BsnI GGCC 5 cut(s) 176, 821, 1049, 1758, 2580
Bso31I GGTCTC 2 cut(s) 60, 3636
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 7 cut(s) 204, 470, 859, 1856, 2607, 3414, 3518
Bsp1720I GCTNAGC 2 cut(s) 328, 3380
BspACI CCGC 7 cut(s) 152, 342, 2081, 3191, 3280, 3390, 3453
BspANI GGCC 5 cut(s) 176, 821, 1049, 1758, 2580
BspFNI CGCG 1 cut(s) 3455
BspHI TCATGA 1 cut(s) 2833
BspLI GGNNCC 9 cut(s) 485, 1378, 1644, 1853, 2067, 2351, 2948, 3120, 3301
BspMAI CTGCAG 1 cut(s) 3082
BspMI ACCTGC 3 cut(s) 508, 3052, 3071
BspPI GGATC 9 cut(s) 924, 1371, 1384, 1397, 1637, 1650, 1965, 2040, 3062
BspQI GCTCTTC 1 cut(s) 3528
BspT107I GGYRCC 1 cut(s) 2349
BspTI CTTAAG 1 cut(s) 530
BspTNI GGTCTC 2 cut(s) 60, 3636
BsrDI GCAATG 2 cut(s) 339, 3324
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 4 cut(s) 70, 552, 1050, 2020
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 6 cut(s) 171, 302, 1879, 3337, 3459, 3535
BssNAI GTATAC 1 cut(s) 498
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 4 cut(s) 302, 3337, 3459, 3535
Bst1107I GTATAC 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 5 cut(s) 846, 1403, 1428, 2595, 2700
Bst6I CTCTTC 4 cut(s) 1040, 1293, 2847, 3528
BstAFI CTTAAG 1 cut(s) 530
BstBAI YACGTR 3 cut(s) 656, 854, 2452
BstC8I GCNNGC 5 cut(s) 156, 1164, 1687, 1756, 2993
BstDSI CCRYGG 2 cut(s) 171, 1879
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 9 cut(s) 295, 317, 646, 1301, 2179, 2228, 2695, 2845, 3211
BstFNI CGCG 1 cut(s) 3455
BstH2I RGCGCY 3 cut(s) 26, 447, 724
BstHHI GCGC 3 cut(s) 25, 446, 723
BstMAI GTCTC 5 cut(s) 60, 407, 964, 3065, 3636
BstMCI CGRYCG 1 cut(s) 3126
BstMWI GCNNNNNNNGC 7 cut(s) 229, 441, 452, 650, 3390, 3402, 3527
BstNI CCWGG 5 cut(s) 846, 1403, 1428, 2595, 2700
BstNSI RCATGY 3 cut(s) 1166, 1792, 2891
BstPAI GACNNNNGTC 1 cut(s) 2641
BstSCI CCNGG 6 cut(s) 844, 1401, 1426, 2593, 2698, 3120
BstSFI CTRYAG 1 cut(s) 3078
BstSLI GKGCMC 1 cut(s) 3518
BstUI CGCG 1 cut(s) 3455
BstV1I GCAGC 7 cut(s) 351, 937, 953, 1261, 2544, 3301, 3405
BstX2I RGATCY 4 cut(s) 1376, 1642, 1957, 3088
BstYI RGATCY 4 cut(s) 1376, 1642, 1957, 3088
BstZ17I GTATAC 1 cut(s) 498
BsuI GTATCC 2 cut(s) 1111, 2370
BsuRI GGCC 5 cut(s) 176, 821, 1049, 1758, 2580
BtgI CCRYGG 2 cut(s) 171, 1879
BtgZI GCGATG 3 cut(s) 138, 1698, 2546
BtrI CACGTC 1 cut(s) 2755
BtsCI GGATG 9 cut(s) 295, 317, 646, 1301, 2179, 2228, 2695, 2845, 3211
BtsI GCAGTG 1 cut(s) 3516
BtsIMutI CAGTG 8 cut(s) 158, 559, 765, 1057, 2271, 2639, 3477, 3516
BveI ACCTGC 3 cut(s) 508, 3052, 3071
Cac8I GCNNGC 5 cut(s) 156, 1164, 1687, 1756, 2993
CaiI CAGNNNCTG 2 cut(s) 1172, 2222
CciI TCATGA 1 cut(s) 2833
CfoI GCGC 3 cut(s) 25, 446, 723
Cfr10I RCCGGY 1 cut(s) 154
CseI GACGC 1 cut(s) 3628
Csp6I GTAC 7 cut(s) 31, 657, 1554, 1969, 2350, 2447, 3447
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 7 cut(s) 31, 657, 1554, 1969, 2350, 2447, 3447
DraI TTTAAA 1 cut(s) 2463
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 4 cut(s) 1040, 1293, 2847, 3528
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 4 cut(s) 1040, 1293, 2847, 3528
Eco130I CCWWGG 4 cut(s) 302, 3337, 3459, 3535
Eco24I GRGCYC 4 cut(s) 470, 1856, 2607, 3414
Eco31I GGTCTC 2 cut(s) 60, 3636
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 9 cut(s) 94, 973, 2591, 2636, 2906, 2946, 3118, 3300, 3322
Eco47III AGCGCT 1 cut(s) 722
Eco57I CTGAAG 6 cut(s) 681, 1274, 1496, 1745, 2090, 3581
Eco88I CYCGRG 1 cut(s) 18
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoO109I RGGNCCY 2 cut(s) 2946, 3118
EcoRI GAATTC 3 cut(s) 545, 1780, 3273
EcoRII CCWGG 5 cut(s) 844, 1401, 1426, 2593, 2698
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 4 cut(s) 302, 3337, 3459, 3535
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 4 cut(s) 470, 1856, 2607, 3414
ErhI CCWWGG 4 cut(s) 302, 3337, 3459, 3535
FaqI GGGAC 2 cut(s) 99, 1250
FauNDI CATATG 1 cut(s) 2916
FblI GTMKAC 3 cut(s) 497, 2268, 2751
Fnu4HI GCNGC 9 cut(s) 152, 340, 926, 942, 1250, 2558, 3315, 3391, 3394
FokI GGATG 9 cut(s) 302, 304, 653, 1288, 2186, 2215, 2702, 2852, 3218
FriOI GRGCYC 4 cut(s) 470, 1856, 2607, 3414
Fsp4HI GCNGC 9 cut(s) 152, 340, 926, 942, 1250, 2558, 3315, 3391, 3394
FspBI CTAG 6 cut(s) 324, 447, 1707, 1733, 2337, 3536
GlaI GCGC 3 cut(s) 24, 445, 722
GluI GCNGC 9 cut(s) 152, 340, 926, 942, 1250, 2558, 3315, 3391, 3394
GsuI CTGGAG 7 cut(s) 46, 47, 290, 535, 1584, 1655, 3314
HaeII RGCGCY 3 cut(s) 26, 447, 724
HaeIII GGCC 5 cut(s) 176, 821, 1049, 1758, 2580
HapII CCGG 2 cut(s) 155, 3122
HgaI GACGC 1 cut(s) 3628
HhaI GCGC 3 cut(s) 25, 446, 723
Hin6I GCGC 3 cut(s) 23, 444, 721
HinP1I GCGC 3 cut(s) 23, 444, 721
HincII GTYRAC 2 cut(s) 1349, 1464
HindII GTYRAC 2 cut(s) 1349, 1464
HindIII AAGCTT 4 cut(s) 747, 1010, 2086, 2412
HpaII CCGG 2 cut(s) 155, 3122
HphI GGTGA 7 cut(s) 450, 1462, 1596, 2807, 3118, 3320, 3360
HpyCH4IV ACGT 8 cut(s) 165, 655, 853, 949, 2436, 2451, 2754, 2873
HpyF10VI GCNNNNNNNGC 7 cut(s) 229, 441, 452, 650, 3390, 3402, 3527
HpySE526I ACGT 8 cut(s) 165, 655, 853, 949, 2436, 2451, 2754, 2873
HspAI GCGC 3 cut(s) 23, 444, 721
KpnI GGTACC 1 cut(s) 2353
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
LguI GCTCTTC 1 cut(s) 3528
LmnI GCTCC 4 cut(s) 207, 718, 1565, 1851
Lsp1109I GCAGC 7 cut(s) 351, 937, 953, 1261, 2544, 3301, 3405
MaeI CTAG 6 cut(s) 324, 447, 1707, 1733, 2337, 3536
MaeII ACGT 8 cut(s) 165, 655, 853, 949, 2436, 2451, 2754, 2873
MfeI CAATTG 2 cut(s) 963, 1677
MflI RGATCY 4 cut(s) 1376, 1642, 1957, 3088
MhlI GDGCHC 7 cut(s) 204, 470, 859, 1856, 2607, 3414, 3518
MlyI GAGTC 4 cut(s) 14, 307, 409, 1610
MmeI TCCRAC 4 cut(s) 163, 171, 1947, 3219
Mph1103I ATGCAT 1 cut(s) 1164
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 56
MslI CAYNNNNRTG 2 cut(s) 2295, 2838
MspA1I CMGCKG 2 cut(s) 342, 2081
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 2 cut(s) 155, 3122
MspR9I CCNGG 6 cut(s) 846, 1403, 1428, 2595, 2700, 3122
MunI CAATTG 2 cut(s) 963, 1677
Mva1269I GAATGC 2 cut(s) 133, 2154
MvaI CCWGG 5 cut(s) 846, 1403, 1428, 2595, 2700
MvnI CGCG 1 cut(s) 3455
MwoI GCNNNNNNNGC 7 cut(s) 229, 441, 452, 650, 3390, 3402, 3527
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 3122
NdeI CATATG 1 cut(s) 2916
NgoMIV GCCGGC 1 cut(s) 154
NlaIV GGNNCC 9 cut(s) 485, 1378, 1644, 1853, 2067, 2351, 2948, 3120, 3301
NmuCI GTSAC 5 cut(s) 554, 1489, 1520, 1602, 1728
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 3 cut(s) 1166, 1792, 2891
PaeI GCATGC 1 cut(s) 1166
PagI TCATGA 1 cut(s) 2833
PciSI GCTCTTC 1 cut(s) 3528
PctI GAATGC 2 cut(s) 133, 2154
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 56
PflFI GACNNNGTC 1 cut(s) 3122
PflMI CCANNNNNTGG 1 cut(s) 2705
PfoI TCCNGGA 2 cut(s) 2593, 2698
PkrI GCNGC 9 cut(s) 153, 341, 927, 943, 1251, 2559, 3316, 3392, 3395
PleI GAGTC 4 cut(s) 13, 306, 409, 1609
PpsI GAGTC 4 cut(s) 13, 306, 409, 1609
Ppu21I YACGTR 3 cut(s) 656, 854, 2452
PpuMI RGGWCCY 2 cut(s) 2946, 3118
PshAI GACNNNNGTC 1 cut(s) 2641
PshBI ATTAAT 1 cut(s) 1341
PsiI TTATAA 1 cut(s) 2186
Psp1406I AACGTT 1 cut(s) 2873
Psp5II RGGWCCY 2 cut(s) 2946, 3118
Psp6I CCWGG 5 cut(s) 844, 1401, 1426, 2593, 2698
PspGI CCWGG 5 cut(s) 844, 1401, 1426, 2593, 2698
PspN4I GGNNCC 9 cut(s) 485, 1378, 1644, 1853, 2067, 2351, 2948, 3120, 3301
PspPPI RGGWCCY 2 cut(s) 2946, 3118
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 1 cut(s) 3082
PstNI CAGNNNCTG 2 cut(s) 1172, 2222
PsuI RGATCY 4 cut(s) 1376, 1642, 1957, 3088
PsyI GACNNNGTC 1 cut(s) 3122
RsaI GTAC 7 cut(s) 32, 658, 1555, 1970, 2351, 2448, 3448
RsaNI GTAC 7 cut(s) 31, 657, 1554, 1969, 2350, 2447, 3447
RseI CAYNNNNRTG 2 cut(s) 2295, 2838
SapI GCTCTTC 1 cut(s) 3528
SatI GCNGC 9 cut(s) 152, 340, 926, 942, 1250, 2558, 3315, 3391, 3394
SchI GAGTC 4 cut(s) 14, 307, 409, 1610
ScrFI CCNGG 6 cut(s) 846, 1403, 1428, 2595, 2700, 3122
SduI GDGCHC 7 cut(s) 204, 470, 859, 1856, 2607, 3414, 3518
SfcI CTRYAG 1 cut(s) 3078
SinI GGWCC 9 cut(s) 94, 973, 2591, 2636, 2906, 2946, 3118, 3300, 3322
SmiMI CAYNNNNRTG 2 cut(s) 2295, 2838
SmlI CTYRAG 6 cut(s) 410, 530, 587, 794, 3287, 3482
SmoI CTYRAG 6 cut(s) 410, 530, 587, 794, 3287, 3482
SpeI ACTAGT 1 cut(s) 2336
SphI GCATGC 1 cut(s) 1166
SsiI CCGC 7 cut(s) 152, 342, 2081, 3191, 3280, 3390, 3453
SspI AATATT 2 cut(s) 44, 2140
SspMI CTAG 6 cut(s) 324, 447, 1707, 1733, 2337, 3536
StyD4I CCNGG 6 cut(s) 844, 1401, 1426, 2593, 2698, 3120
StyI CCWWGG 4 cut(s) 302, 3337, 3459, 3535
TaiI ACGT 8 cut(s) 168, 658, 856, 952, 2439, 2454, 2757, 2876
TatI WGTACW 2 cut(s) 30, 3446
TauI GCSGC 2 cut(s) 154, 3393
TscAI CASTG 8 cut(s) 165, 559, 765, 1057, 2278, 2646, 3477, 3523
TseFI GTSAC 5 cut(s) 554, 1489, 1520, 1602, 1728
TseI GCWGC 7 cut(s) 339, 925, 941, 1249, 2557, 3314, 3393
Tsp45I GTSAC 5 cut(s) 554, 1489, 1520, 1602, 1728
TspDTI ATGAA 8 cut(s) 453, 522, 556, 625, 1565, 2217, 2481, 2552
TspGWI ACGGA 4 cut(s) 103, 496, 2122, 2749
TspRI CASTG 8 cut(s) 165, 559, 765, 1057, 2278, 2646, 3477, 3523
Tth111I GACNNNGTC 1 cut(s) 3122
Van91I CCANNNNNTGG 1 cut(s) 2705
Vha464I CTTAAG 1 cut(s) 530
VneI GTGCAC 1 cut(s) 3514
VpaK11BI GGWCC 9 cut(s) 94, 973, 2591, 2636, 2906, 2946, 3118, 3300, 3322
VspI ATTAAT 1 cut(s) 1341
XagI CCTNNNNNAGG 1 cut(s) 1384
XapI RAATTY 8 cut(s) 545, 735, 1780, 2114, 3095, 3164, 3273, 3424
XceI RCATGY 3 cut(s) 1166, 1792, 2891
XcmI CCANNNNNNNNNTGG 1 cut(s) 1228
XmaJI CCTAGG 1 cut(s) 3535
XmiI GTMKAC 3 cut(s) 497, 2268, 2751
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 6 cut(s) 324, 447, 1707, 1733, 2337, 3536
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.