Rh5AG253800

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007959
Physical Location & Seq
Forward (+)
32707030 .. 32715746
8717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG253800.1

Sequence Viewer

Length: 3144 bp
ATGGAGACCCTTCTGGGTCGAGCGCTGGAGTATACTCTCAAGTACTGGCTCAAATCCTTCTCCAGAGACCAGTTCAAGTGGCAGGGACGGACGGTTCAGCTTTCCAATTTAGATATGGACGGCGATGCTTTGCACTCCAGCATGGGATTACCGCCGGCGCTGCACGTGACCACGGCCAGAGTCGGCAAACTTGTGATAGTGCTCCCATCATCTGTGAGCAATGTTCAAGTGGAACCGATAGTAGTGCAGATTGATAAGCTGGATTTGGTTTTAGAGGAGACTTCGGAGTTGGATGCGAGAAACAGTCCTAGCAGTTCGCCATCATCCACTAGCTCCGGGAAAGGAAGTGGTTATGGATTTGCTGATAAGATTGCAGATGGAATGACTATAGAGATTCACACAGTTAATCTTCTACTTGAAACTTGTGGGGGTGGTCGCCAAGGAGGGGCAGCTTGGGCATCTCCTCTGGCTTCTATCACTATACACAACCTTTTGCTGTATTCTACTAATGAAAATTGGCAGGTAGTAAATCTTAAGGAAGCACGGGAGTTCTCCACCAACAAAAAGTTCATTTATGTTTTCAAAAAACTTGAGTGGGAATCTTTGTCCATCGATCTCCTGCCTCACCCTGATATGTTCACGGATGCTAATATAGCGAGCACTCAAAATGGAGGAACCCAGAGAGATGATGATGGTGCAAAGCGAGCTTTCTTTGGTGGGGAGCGCTTTATAGAAGGAATTTCAGGAGAAGCTTATATCACAGTGCAAAGGACTGAATTAAATAGTCCACTCGGTCTTGAGGTTCAGTTACATATCACAGAAGCCATTTGTCCTGCAATAAGTGAACCAGGACTACGTGCTCTTCTCCGCTTTATGACTGGATTATATGTCTGTCTGAATAGAGGAGATGTTGATTCAAATACTCAGAAGGTTTGCAGTTTCCTTTACTTTATTTACTTACCATTATCATGCTTAGGGACCATCTTAATTTCCCCTTGTCTTTCTATCCGTGTGGTATTTTTTCAGCGGTCAACTGAAGCAGCAGGCCGTTCTATCGTTTCAATTGTTGTAGATCACATATTTCTCTGTATTAAAGATACTGAATTCAAGCTTGAACTTTTGATGCAGTCACTCTTCTTTTCACGGACAAGTGTTTCTGATGGAGAAATTGACAATAACTTGTCTAAGGTTATGATTGGTGGGCTCTTTTTAAGGGATACCTATTCAAGACCTCCTTGCACTTTAGTGCAACCATCAATGGATGCCATTTCCGACGAACCTGTGCATGTTCCTGACTTTGGTAAGGACTTTTGCCCTCCCATATATCCTTTGGGAGCTCAGCAGTGGCAGTTGATCGTGGGTGTCCCTCTTTTATGCCTTCATTCTCTTCAGACCAAGCCCTCCCCTGTGCCTCCAGCTTTTGCTACACAAACAGTCATTAATTGCCAACCCCTGATGATTCATCTTCAGGAAGAATCCTGTTTGAGGATCTCTTCCTTCCTGGCCGATGGAATTGTTGTCAATCCCGGTGCTGTTTTGCCAGATTTCTCAGTAAATTCCTTGATTTTCACCCTCAAGGAATTAGATGTTACCGTTCCTTTGGACGTGGACAATTTGCGTAGTCGTGGTAACAACAGAAGCAGTATCAACCAGAGTTCCTTTTCCGGAGCAAGGCTTCACATTAAGAACTTGTTCTTCTCAGAGTCACCGTCACTAAAACTAAGGCTACTGAATTTGGACAAGGATCCTGCTTGCTTCTGTCTCTGGGAAGGTCAACCAGTTGATGCTAGCCAGAAGAAATGGACTACCAGAGCATCACACCTTACTTTGTCTCTCGAAACGTGTACTGCATCAGCTGGACTTCAGAGTTCTCTTGATGGGACCTCAGGCTTGTGGAGATGTGTTGAGCTGAAAGATGCTTGCATAGAAGTAGCTATGGTAACTGCTGATGGGAGCCCATTAACAAATGTTCCTCCTCCTGGTGGTATTGTCAGGATAGGGCTAGCTTGTGAAAAGTATATCTCAAATACTTCTGTTGAACAGTTGTATTTTGTTTTGGATCTTTATGCGTACTTTGGCAGAGTTAGTGAAAAGATAGTTCTTGTTGGAAAATATACTAGACCAAAGACGAGTACGGATGATACGTTCAAGGGGAAGCTAATTGACAAGGTTCCCAATGATACAGCAGTTAGTTTAATGGTGAATAATCTTCAGCTACGATTTCTGGAATCATCTTCCATGCATGTTGAAGGAACGCCTCTAGTTCAGTTTGTTGGGCATGATCTCTTTATAAGAGTTACTCACAGAACTCTTGGTGGTGCAGTTGCTGTTTCCTCCATTATACGTTGGGATAGCGTTGAGGTGGACTGCATAGACAGTGAGGGAAACTTGGCATCTGAGAATGGCAGAGATTTAATTTCTATTGAAAATGGTCATGGTGTTTTGGTGAGTGGAAATGGGTATCCTCAACTAAGACCTGTCTTTTGGATAGATAACCAAATAAACCATTCATCAAATGGTAAAGCTATTGTAGATCGATTTCTTGACATAAACGTGGAACATGTAATTCCTTTAAATGAACAAGATGTAGAGTGTCACAGTTTGAATGTGTCAGCTTGTATTTCTGGCATTCGCCTAGGTGGCGGAATGAACTATGCTGAATCCTTGCTGCATCGCTTTGGAATACTTGAGGCTGATGGTGGTCCTGGGAAGGGGCTATCTAAAGAGTTGGAGAATTTACGAGCAGGCCCCCTTTCAAAAATTTTTGAACCATCACCTCTCATTGCTGATTCAAAAGAGGATGGAAGTTCAGGAGATGGGAAAGAGAGTGAGGTTTTGCACTTGGGAAAGCCAGATGATGTGAATGTGTCCATAGAATTGAAAAATTGGTTATTTGCTCTTGAAGGCGCACAGGAAATGGCAGTGTTTAGAAGTTCCTCCAATCAGGAGGGTGATAGCAGAGAAGAGAGGTCTTGGCACACAACATTCCACAATCTGCACCTAAAAGGAAAAAGCCGTCCAAAACAAATGATGAATGGCATTAGAAAGTCATACAGGACACCAAAATATCCTTTAGAATTGGTGACTGTAAGCATATCTCTCTCTTCCCTTGATCCCTATGTTTTTAATATCCTGTTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1047

Amino Acids

115.04

Weight (kDa)

5.46

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 33 - 205 5.3e-12 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2291
Acc36I ACCTGC 1 cut(s) 511
AccI GTMKAC 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 1664
AciI CCGC 4 cut(s) 152, 868, 1027, 2643
AclWI GGATC 5 cut(s) 1496, 1739, 1752, 2067, 3107
AcoI YGGCCR 2 cut(s) 174, 1503
AcsI RAATTY 6 cut(s) 738, 1103, 1555, 1732, 2734, 2760
AcuI CTGAAG 5 cut(s) 1056, 1373, 1451, 1847, 2195
AcvI CACGTG 1 cut(s) 166
AfaI GTAC 4 cut(s) 44, 1846, 2072, 2134
AfeI AGCGCT 2 cut(s) 24, 725
AfiI CCNNNNNNNGG 7 cut(s) 445, 1298, 1485, 1671, 1979, 1982, 2711
AflII CTTAAG 1 cut(s) 533
AflIII ACRYGT 2 cut(s) 1841, 2560
AjiI CACGTC 1 cut(s) 1606
AjnI CCWGG 4 cut(s) 847, 1500, 1978, 2704
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AleI CACNNNNGTG 1 cut(s) 1244
AloI GAACNNNNNNTCC 4 cut(s) 78, 110, 2129, 2161
Alw21I GWGCWC 4 cut(s) 204, 662, 862, 1339
Alw26I GTCTC 4 cut(s) 60, 272, 1766, 1836
AlwI GGATC 5 cut(s) 1496, 1739, 1752, 2067, 3107
AlwNI CAGNNNCTG 1 cut(s) 2327
Aor13HI TCCGGA 1 cut(s) 1664
Aor51HI AGCGCT 2 cut(s) 24, 725
AoxI GGCC 4 cut(s) 174, 1045, 1503, 2746
ApeKI GCWGC 4 cut(s) 160, 449, 1040, 2668
ApoI RAATTY 6 cut(s) 738, 1103, 1555, 1732, 2734, 2760
ArsI GACNNNNNNTTYG 2 cut(s) 2466, 2498
AseI ATTAAT 1 cut(s) 1440
Asp700I GAANNNNTTC 1 cut(s) 1691
AspA2I CCTAGG 1 cut(s) 2635
AspLEI GCGC 4 cut(s) 25, 160, 726, 2909
AspS9I GGNCC 4 cut(s) 978, 1881, 2702, 2747
AsuC2I CCSGG 2 cut(s) 337, 1527
AsuHPI GGTGA 8 cut(s) 617, 1561, 1698, 2212, 2458, 2766, 2963, 3094
AsuNHI GCTAGC 2 cut(s) 1787, 2002
AvaII GGWCC 3 cut(s) 978, 1881, 2702
AvrII CCTAGG 1 cut(s) 2635
AxyI CCTNAGG 1 cut(s) 1885
BaeI ACNNNNGTAYC 2 cut(s) 2172, 2205
BamHI GGATCC 1 cut(s) 1744
BanII GRGCYC 3 cut(s) 1206, 1339, 1958
BbrPI CACGTG 1 cut(s) 166
Bbv12I GWGCWC 4 cut(s) 204, 662, 862, 1339
BbvI GCAGC 4 cut(s) 147, 461, 1052, 2655
BceAI ACGGC 4 cut(s) 136, 189, 1032, 3000
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 4 cut(s) 849, 1502, 1980, 2706
BciVI GTATCC 2 cut(s) 1210, 2472
BcnI CCSGG 2 cut(s) 337, 1527
BcoDI GTCTC 4 cut(s) 60, 272, 1766, 1836
BfaI CTAG 8 cut(s) 309, 330, 1788, 2003, 2118, 2261, 2636, 3142
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 3 cut(s) 26, 161, 727
BfrI CTTAAG 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 511
BfuI GTATCC 2 cut(s) 1210, 2472
BglI GCCNNNNNGGC 1 cut(s) 2640
BisI GCNGC 4 cut(s) 161, 450, 1041, 2669
BlnI CCTAGG 1 cut(s) 2635
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 4 cut(s) 162, 451, 1042, 2670
BmcAI AGTACT 1 cut(s) 44
Bme1390I CCNGG 6 cut(s) 337, 849, 1502, 1527, 1980, 2706
Bme18I GGWCC 3 cut(s) 978, 1881, 2702
BmgBI CACGTC 1 cut(s) 1606
BmgT120I GGNCC 4 cut(s) 978, 1881, 2702, 2747
BmiI GGNNCC 8 cut(s) 234, 676, 979, 1746, 1882, 1955, 2172, 2749
BmrFI CCNGG 6 cut(s) 337, 849, 1502, 1527, 1980, 2706
BmtI GCTAGC 2 cut(s) 1791, 2006
BpmI CTGGAG 4 cut(s) 46, 47, 121, 1398
Bpu10I CCTNAGC 1 cut(s) 973
Bpu1102I GCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 5 cut(s) 23, 611, 818, 1559, 2708
BpuMI CCSGG 2 cut(s) 337, 1527
Bsa29I ATCGAT 2 cut(s) 612, 2536
BsaAI YACGTR 2 cut(s) 166, 857
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 4 cut(s) 171, 439, 2635, 2705
BsaWI WCCGGW 1 cut(s) 1664
Bsc4I CCNNNNNNNGG 7 cut(s) 445, 1298, 1485, 1671, 1979, 1982, 2711
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 4 cut(s) 50, 70, 883, 1778
Bse21I CCTNAGG 1 cut(s) 1885
Bse3DI GCAATG 2 cut(s) 226, 2781
BseAI TCCGGA 1 cut(s) 1664
BseBI CCWGG 4 cut(s) 849, 1502, 1980, 2706
BseCI ATCGAT 2 cut(s) 612, 2536
BseDI CCNNGG 4 cut(s) 171, 439, 2635, 2705
BseGI GGATG 6 cut(s) 298, 323, 649, 1267, 2143, 2806
BseLI CCNNNNNNNGG 7 cut(s) 445, 1298, 1485, 1671, 1979, 1982, 2711
BseMI GCAATG 2 cut(s) 226, 2781
BseMII CTCAG 6 cut(s) 938, 1352, 1563, 1713, 1899, 2388
BseNI ACTGG 4 cut(s) 50, 70, 883, 1778
BseRI GAGGAG 4 cut(s) 290, 453, 918, 1965
BseXI GCAGC 4 cut(s) 147, 461, 1052, 2655
BsgI GTGCAG 4 cut(s) 146, 266, 2340, 2981
BshFI GGCC 4 cut(s) 176, 1047, 1505, 2748
BshVI ATCGAT 2 cut(s) 612, 2536
BsiHKAI GWGCWC 4 cut(s) 204, 662, 862, 1339
BsiSI CCGG 4 cut(s) 155, 336, 1527, 1665
BslFI GGGAC 4 cut(s) 99, 991, 1349, 1894
BslI CCNNNNNNNGG 7 cut(s) 445, 1298, 1485, 1671, 1979, 1982, 2711
BsmAI GTCTC 4 cut(s) 60, 272, 1766, 1836
BsmFI GGGAC 4 cut(s) 99, 991, 1349, 1894
BsmI GAATGC 1 cut(s) 2628
BsnI GGCC 4 cut(s) 176, 1047, 1505, 2748
Bso31I GGTCTC 1 cut(s) 60
Bsp1286I GDGCHC 6 cut(s) 204, 662, 862, 1206, 1339, 1958
Bsp13I TCCGGA 1 cut(s) 1664
Bsp143I GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
Bsp1720I GCTNAGC 1 cut(s) 1338
BspACI CCGC 4 cut(s) 152, 868, 1027, 2643
BspANI GGCC 4 cut(s) 176, 1047, 1505, 2748
BspCNI CTCAG 6 cut(s) 937, 1351, 1562, 1712, 1898, 2389
BspDI ATCGAT 2 cut(s) 612, 2536
BspEI TCCGGA 1 cut(s) 1664
BspLI GGNNCC 8 cut(s) 234, 676, 979, 1746, 1882, 1955, 2172, 2749
BspMI ACCTGC 1 cut(s) 511
BspOI GCTAGC 2 cut(s) 1791, 2006
BspPI GGATC 5 cut(s) 1496, 1739, 1752, 2067, 3107
BspQI GCTCTTC 1 cut(s) 867
BspTI CTTAAG 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 2 cut(s) 226, 2781
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 4 cut(s) 50, 70, 883, 1778
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 4 cut(s) 171, 439, 2635, 2705
BssMI GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
BssNAI GTATAC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 439, 2635
Bst1107I GTATAC 1 cut(s) 33
Bst2UI CCWGG 4 cut(s) 849, 1502, 1980, 2706
Bst6I CTCTTC 6 cut(s) 867, 1139, 1392, 1498, 2958, 3109
BstAFI CTTAAG 1 cut(s) 533
BstBAI YACGTR 2 cut(s) 166, 857
BstC8I GCNNGC 9 cut(s) 156, 658, 705, 1045, 1753, 1789, 1921, 2004, 2746
BstDSI CCRYGG 1 cut(s) 171
BstENI CCTNNNNNAGG 1 cut(s) 1483
BstF5I GGATG 6 cut(s) 298, 323, 649, 1267, 2143, 2806
BstH2I RGCGCY 3 cut(s) 26, 161, 727
BstHHI GCGC 4 cut(s) 25, 160, 726, 2909
BstKTI GATC 9 cut(s) 616, 1075, 1356, 1491, 1747, 2062, 2284, 2536, 3115
BstMAI GTCTC 4 cut(s) 60, 272, 1766, 1836
BstMBI GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
BstMWI GCNNNNNNNGC 5 cut(s) 160, 455, 653, 704, 2640
BstNI CCWGG 4 cut(s) 849, 1502, 1980, 2706
BstNSI RCATGY 3 cut(s) 1289, 2246, 2564
BstSCI CCNGG 6 cut(s) 335, 847, 1500, 1525, 1978, 2704
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 4 cut(s) 147, 461, 1052, 2655
BstX2I RGATCY 3 cut(s) 1488, 1744, 2059
BstYI RGATCY 3 cut(s) 1488, 1744, 2059
BstZ17I GTATAC 1 cut(s) 33
Bsu15I ATCGAT 2 cut(s) 612, 2536
Bsu36I CCTNAGG 1 cut(s) 1885
BsuI GTATCC 2 cut(s) 1210, 2472
BsuRI GGCC 4 cut(s) 176, 1047, 1505, 2748
BsuTUI ATCGAT 2 cut(s) 612, 2536
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 2 cut(s) 138, 2657
BtrI CACGTC 1 cut(s) 1606
BtsCI GGATG 6 cut(s) 298, 323, 649, 1267, 2143, 2806
BtsI GCAGTG 2 cut(s) 1349, 2928
BtsIMutI CAGTG 4 cut(s) 768, 1349, 2383, 2928
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 9 cut(s) 156, 658, 705, 1045, 1753, 1789, 1921, 2004, 2746
CaiI CAGNNNCTG 1 cut(s) 2327
CfoI GCGC 4 cut(s) 25, 160, 726, 2909
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 978, 1881, 2702, 2747
ClaI ATCGAT 2 cut(s) 612, 2536
Csp6I GTAC 4 cut(s) 43, 1845, 2071, 2133
CviAII CATG 8 cut(s) 142, 969, 1286, 2239, 2243, 2279, 2435, 2561
CviQI GTAC 4 cut(s) 43, 1845, 2071, 2133
DpnI GATC 9 cut(s) 615, 1074, 1355, 1490, 1746, 2061, 2283, 2535, 3114
DpnII GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
DraI TTTAAA 1 cut(s) 2574
EaeI YGGCCR 2 cut(s) 174, 1503
Eam1104I CTCTTC 6 cut(s) 867, 1139, 1392, 1498, 2958, 3109
EarI CTCTTC 6 cut(s) 867, 1139, 1392, 1498, 2958, 3109
EciI GGCGGA 1 cut(s) 2658
Ecl136II GAGCTC 1 cut(s) 1337
Eco130I CCWWGG 2 cut(s) 439, 2635
Eco24I GRGCYC 3 cut(s) 1206, 1339, 1958
Eco31I GGTCTC 1 cut(s) 60
Eco47I GGWCC 3 cut(s) 978, 1881, 2702
Eco47III AGCGCT 2 cut(s) 24, 725
Eco53kI GAGCTC 1 cut(s) 1337
Eco57I CTGAAG 5 cut(s) 1056, 1373, 1451, 1847, 2195
Eco72I CACGTG 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 1885
EcoICRI GAGCTC 1 cut(s) 1337
EcoNI CCTNNNNNAGG 1 cut(s) 1483
EcoO109I RGGNCCY 2 cut(s) 1881, 2747
EcoRI GAATTC 1 cut(s) 1103
EcoRII CCWGG 4 cut(s) 847, 1500, 1978, 2704
EcoT14I CCWWGG 2 cut(s) 439, 2635
EcoT22I ATGCAT 1 cut(s) 2244
EcoT38I GRGCYC 3 cut(s) 1206, 1339, 1958
ErhI CCWWGG 2 cut(s) 439, 2635
FaeI CATG 8 cut(s) 145, 972, 1289, 2242, 2246, 2282, 2438, 2564
FalI AAGNNNNNCTT 2 cut(s) 1219, 1251
FaqI GGGAC 4 cut(s) 99, 991, 1349, 1894
FatI CATG 8 cut(s) 141, 968, 1285, 2238, 2242, 2278, 2434, 2560
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 161, 450, 1041, 2669
FokI GGATG 6 cut(s) 305, 310, 656, 1274, 2150, 2813
FriOI GRGCYC 3 cut(s) 1206, 1339, 1958
Fsp4HI GCNGC 4 cut(s) 161, 450, 1041, 2669
FspBI CTAG 8 cut(s) 309, 330, 1788, 2003, 2118, 2261, 2636, 3142
GlaI GCGC 4 cut(s) 24, 159, 725, 2908
GluI GCNGC 4 cut(s) 161, 450, 1041, 2669
GsuI CTGGAG 4 cut(s) 46, 47, 121, 1398
HaeII RGCGCY 3 cut(s) 26, 161, 727
HaeIII GGCC 4 cut(s) 176, 1047, 1505, 2748
HapII CCGG 4 cut(s) 155, 336, 1527, 1665
HhaI GCGC 4 cut(s) 25, 160, 726, 2909
Hin1II CATG 8 cut(s) 145, 972, 1289, 2242, 2246, 2282, 2438, 2564
Hin6I GCGC 4 cut(s) 23, 158, 724, 2907
HinP1I GCGC 4 cut(s) 23, 158, 724, 2907
HincII GTYRAC 2 cut(s) 1032, 1775
HindII GTYRAC 2 cut(s) 1032, 1775
HindIII AAGCTT 2 cut(s) 750, 1109
HpaII CCGG 4 cut(s) 155, 336, 1527, 1665
HphI GGTGA 8 cut(s) 617, 1561, 1698, 2212, 2458, 2766, 2963, 3094
Hpy166II GTNNAC 9 cut(s) 33, 639, 788, 845, 1032, 1609, 1775, 1845, 2365
Hpy188I TCNGA 9 cut(s) 286, 897, 927, 1159, 1273, 1392, 1702, 1866, 2398
Hpy8I GTNNAC 9 cut(s) 33, 639, 788, 845, 1032, 1609, 1775, 1845, 2365
Hpy99I CGWCG 1 cut(s) 1277
HpyCH4IV ACGT 7 cut(s) 165, 856, 1605, 1841, 2144, 2344, 2553
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 455, 653, 704, 2640
HpySE526I ACGT 7 cut(s) 165, 856, 1605, 1841, 2144, 2344, 2553
Hsp92II CATG 8 cut(s) 145, 972, 1289, 2242, 2246, 2282, 2438, 2564
HspAI GCGC 4 cut(s) 23, 158, 724, 2907
Kpn2I TCCGGA 1 cut(s) 1664
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 6 cut(s) 207, 338, 721, 1334, 1667, 1953
Lsp1109I GCAGC 4 cut(s) 147, 461, 1052, 2655
MaeI CTAG 8 cut(s) 309, 330, 1788, 2003, 2118, 2261, 2636, 3142
MaeII ACGT 7 cut(s) 165, 856, 1605, 1841, 2144, 2344, 2553
MalI GATC 9 cut(s) 615, 1074, 1355, 1490, 1746, 2061, 2283, 2535, 3114
MboI GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
MfeI CAATTG 1 cut(s) 1062
MflI RGATCY 3 cut(s) 1488, 1744, 2059
MhlI GDGCHC 6 cut(s) 204, 662, 862, 1206, 1339, 1958
MlyI GAGTC 2 cut(s) 189, 1712
MmeI TCCRAC 4 cut(s) 270, 1296, 2086, 2709
Mph1103I ATGCAT 1 cut(s) 2244
MreI CGCCGGCG 1 cut(s) 154
MroI TCCGGA 1 cut(s) 1664
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 1691
MslI CAYNNNNRTG 3 cut(s) 967, 1244, 2552
MspA1I CMGCKG 2 cut(s) 1027, 1856
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 4 cut(s) 155, 336, 1527, 1665
MspR9I CCNGG 6 cut(s) 337, 849, 1502, 1527, 1980, 2706
MunI CAATTG 1 cut(s) 1062
Mva1269I GAATGC 1 cut(s) 2628
MvaI CCWGG 4 cut(s) 849, 1502, 1980, 2706
MwoI GCNNNNNNNGC 5 cut(s) 160, 455, 653, 704, 2640
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 2 cut(s) 337, 1527
NdeII GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
NgoMIV GCCGGC 1 cut(s) 154
NheI GCTAGC 2 cut(s) 1787, 2002
NlaIII CATG 8 cut(s) 145, 972, 1289, 2242, 2246, 2282, 2438, 2564
NlaIV GGNNCC 8 cut(s) 234, 676, 979, 1746, 1882, 1955, 2172, 2749
NmuCI GTSAC 6 cut(s) 166, 1128, 1704, 1710, 2594, 3082
NsiI ATGCAT 1 cut(s) 2244
NspI RCATGY 3 cut(s) 1289, 2246, 2564
OliI CACNNNNGTG 1 cut(s) 1244
PciI ACATGT 1 cut(s) 2560
PciSI GCTCTTC 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 2141
PctI GAATGC 1 cut(s) 2628
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 1691
PfeI GAWTC 8 cut(s) 394, 599, 914, 1459, 1475, 2228, 2660, 2789
PfoI TCCNGGA 1 cut(s) 335
PkrI GCNGC 4 cut(s) 162, 451, 1042, 2670
PleI GAGTC 2 cut(s) 188, 1711
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 2 cut(s) 188, 1711
Ppu21I YACGTR 2 cut(s) 166, 857
PpuMI RGGWCCY 1 cut(s) 1881
PscI ACATGT 1 cut(s) 2560
PshBI ATTAAT 1 cut(s) 1440
PsiI TTATAA 1 cut(s) 2291
Psp124BI GAGCTC 1 cut(s) 1339
Psp5II RGGWCCY 1 cut(s) 1881
Psp6I CCWGG 4 cut(s) 847, 1500, 1978, 2704
PspCI CACGTG 1 cut(s) 166
PspGI CCWGG 4 cut(s) 847, 1500, 1978, 2704
PspN4I GGNNCC 8 cut(s) 234, 676, 979, 1746, 1882, 1955, 2172, 2749
PspPI GGNCC 4 cut(s) 978, 1881, 2702, 2747
PspPPI RGGWCCY 1 cut(s) 1881
PsrI GAACNNNNNNTAC 2 cut(s) 837, 869
PstNI CAGNNNCTG 1 cut(s) 2327
PsuI RGATCY 3 cut(s) 1488, 1744, 2059
PvuII CAGCTG 1 cut(s) 1856
RsaI GTAC 4 cut(s) 44, 1846, 2072, 2134
RsaNI GTAC 4 cut(s) 43, 1845, 2071, 2133
RseI CAYNNNNRTG 3 cut(s) 967, 1244, 2552
SacI GAGCTC 1 cut(s) 1339
SapI GCTCTTC 1 cut(s) 867
SatI GCNGC 4 cut(s) 161, 450, 1041, 2669
Sau3AI GATC 9 cut(s) 613, 1072, 1353, 1488, 1744, 2059, 2281, 2533, 3112
Sau96I GGNCC 4 cut(s) 978, 1881, 2702, 2747
ScaI AGTACT 1 cut(s) 44
SchI GAGTC 2 cut(s) 189, 1712
ScrFI CCNGG 6 cut(s) 337, 849, 1502, 1527, 1980, 2706
SduI GDGCHC 6 cut(s) 204, 662, 862, 1206, 1339, 1958
SfcI CTRYAG 1 cut(s) 387
SgrAI CRCCGGYG 1 cut(s) 154
SinI GGWCC 3 cut(s) 978, 1881, 2702
SmiMI CAYNNNNRTG 3 cut(s) 967, 1244, 2552
SmlI CTYRAG 6 cut(s) 38, 533, 590, 797, 1574, 2687
SmoI CTYRAG 6 cut(s) 38, 533, 590, 797, 1574, 2687
SsiI CCGC 4 cut(s) 152, 868, 1027, 2643
SspMI CTAG 8 cut(s) 309, 330, 1788, 2003, 2118, 2261, 2636, 3142
SstI GAGCTC 1 cut(s) 1339
StyD4I CCNGG 6 cut(s) 335, 847, 1500, 1525, 1978, 2704
StyI CCWWGG 2 cut(s) 439, 2635
TaiI ACGT 7 cut(s) 168, 859, 1608, 1844, 2147, 2347, 2556
TaqI TCGA 4 cut(s) 19, 612, 1836, 2536
TaqII GACCGA 1 cut(s) 782
TatI WGTACW 2 cut(s) 42, 1844
TfiI GAWTC 8 cut(s) 394, 599, 914, 1459, 1475, 2228, 2660, 2789
TscAI CASTG 4 cut(s) 768, 1349, 2383, 2928
TseFI GTSAC 6 cut(s) 166, 1128, 1704, 1710, 2594, 3082
TseI GCWGC 4 cut(s) 160, 449, 1040, 2668
Tsp45I GTSAC 6 cut(s) 166, 1128, 1704, 1710, 2594, 3082
TspDTI ATGAA 8 cut(s) 525, 559, 1370, 1451, 2499, 2592, 2663, 3047
TspGWI ACGGA 5 cut(s) 103, 656, 998, 1159, 2150
TspRI CASTG 4 cut(s) 768, 1349, 2383, 2928
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 3 cut(s) 978, 1881, 2702
VspI ATTAAT 1 cut(s) 1440
XagI CCTNNNNNAGG 1 cut(s) 1483
XapI RAATTY 6 cut(s) 738, 1103, 1555, 1732, 2734, 2760
XceI RCATGY 3 cut(s) 1289, 2246, 2564
XcmI CCANNNNNNNNNTGG 3 cut(s) 112, 1327, 2513
XmaJI CCTAGG 1 cut(s) 2635
XmiI GTMKAC 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 1691
XspI CTAG 8 cut(s) 309, 330, 1788, 2003, 2118, 2261, 2636, 3142
ZrmI AGTACT 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 2244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.