FvH4_3g21780

N-terminal region of Chorein or VPS13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007959
Physical Location & Seq
Forward (+)
14821150 .. 14831519
10370 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21780.t1

Sequence Viewer

Length: 3621 bp
ATGGAGTCCATTCTGGCTCGCGCGCTGGAGTACACTCTCAAGTACTGGCTCAAATCCTTCTCCCGAGACCAGTTCAAGCTGCAGGGACGCACTGTGCAGCTCTCCAATCTAGATATGGACGGCGATGCTTTGCACTCTAGCATGGGATTGCCGCCGGCGCTGCACGTCACCACGGCCAGAGTCGGAAAACTTGTGATTGTGCTGCCATCTGTGAGCAATGTGCAAGTGGAACCGATAGTGGTGCAGATTGATAAGCTCGATTTGGTGTTGGAGGAGAATGCCGAGTTGGATGCGAGTAGCAGTCCGAGCAGTTCGCCGTCGTCCGCTACCTCCGGGAAAGGAAGTGGTTATGGATTTGCTGATAAGATTGCAGATGGAATGACTATAGAGATTCGCACAGTTAATATTCTACTTGAAACTCGTGGGGGTGGTCGCCAAGGAGGGGCAGCATGGGCATCGCCTTTGGCATCTATCACTATACGGAACCTTTTGCTATATTCTACTAATGAAAATTGGGAGGTAGTAAATCTTAAGGAAGCACGGGAGTTCTCCACCAACAAAAGGTTCATTTATGTTTTCAAAAAACTTGAGTGGCAATCTTTGTCCATCGATCTCCTGCCTCACCCTGATATGTTCACGGATGCTAATATAGCGTGTACTCAAATGGGAGGAAACCAGAGAGATGATGATGGTGCAAAGCGGGCTTTCTTTGGTGGGGAGCGCTTTATAGAAGGAATTTCAGGAGAAGCTTATATCACAGTGCAAAGGACTGAATTAAATAGTCCACTTGGGCTTGAGGTTCAGTTACATATCACGGAAGCCATTTGTCCTGCAATAAGTGAACCAGGACTACGTGCTCTTCTCCGGTTTATGACTGGATTATATGTCTGTCTGAGTCGAGGAGATATTGATTCAAACACTCAACAGCGGTCAACTCAAGCAGCAGGCCGTTCTATCGTTTCAATTGTTGTGGACCACATATTTCTTTGCATTAAAGATACTGAATTCAAGCTTGAACTTCTGATGCAATCACTCTTCTTTTCACGGGCAAGTGTTTCTGATGGAGGGATTGACAACAACTTGTCGAAGGTTATGATTGGTGGACTCTTTTTAAGGGATACCTTTTCAAGACCTCCTTGCACCTTAGTGCAACCATCAATGCATGCCATTTCTGAGGAACCTGTGCATGTTCCTGACTTTGGTAAGGACTTTTGCCCTCCCATATATCCTTTGGGAGCTCAGCAGTGGCAGTTGATCGAGGGTGTCCCTCTTTTATGCCTTCATTCTCTTCTGACCAAGCCCTCCCCTGAGCCTCCAGCTTTTGCTACACAAACAGTCATTAATTGCCAACCCCTGATGATTCATCTTCAGGAAGGATCCTGTCTGAGGATCTCTTCCTTCCTGGCTGATGGAATTCTCGCCAGTCCTGGTGCTGTTTTACCGGATTTCTCAGTAAATTCCTTGATTTTCATCCTCAAGGAATTAGATGTTACTGTGCCTTTGGACGTGGACAATTTGCGAAGTCGTGGTAACAACAGGAGCAGTATCAATCAGAGTTCCTTTTCTGGAGCAAGGCTTCACATTGAGAACTTGTTCTTCTCAGAGTCACCTTCACTAAAACTAAGGTTACTGAATTTGGACAAGGATCCTGCTTGCTTTTGTCTCTGGAAAGGTCAACCAGTTGATGCTAGCCAGAAGAAATGGACTACTAGATCATCACACATTAGTTTGTCCCTAGAAACATGCACTGCATCAGCTGGACTTCAGAGTTCTCTTGATGGGACCTCAGGCTTGTGGAGATGTATTGAGCTGAAAGATGCTTGCATAGAAGTAGCTATGGTAACTGCTGATGGGAGCCCATTAACAAATGTTCCTCCTCCTGGTGGTATTGTCAGGATAGGGGTAGCGTGTGAAAAGTATCTCTCAAATACTTCTGTTGAACAGTTGTATTTTGTTTTGGATCTTTATGCGTACTTTGGCAGAGTTAGTGAAAAGATAGTTCTTGTTGGAAAGAGTACTAGACCAAAGATCAAGGATGATTCCTTCAAAGGGAGGCTAATTGACAAGGTTCCCAATGATACAGCAGTTAGTTTAGTGGTGAATGATCTTCAGCTACGATTTCTGGAATCATCGTCCATGCAAATTGAAGGAATGCCTCTAGTTCAGTTTGTTGGGCATGATCTCTTTATAAGAGTCACTCACAGAACTCTTGGTGGTGCAGTTGCTGTTTCCTCCACTATACGTTGGGATAGTGTTGAGGTGGACTGCGTAGATAGTGAGGGAAACTTGGCAAGTCAGAATGGCACAGAAAATGGTCATGGTCTTTTGGCGAGTGGAAATGGGTATCCTCAACTAAGACCTGTCTTTTGGATACATAACCAAATAAACCATTTGTCAAATGGTAAAGCTATTGTGGATCGATTTCTTGACATAAGCGTGGAAAATGTAATTCCTTTAAATGAACAAGATGTAGAATGTCATAGTTTGAGTGTGTCAGCTTGTATTTCTGGCATTCGCCTAGGTGGTGGAATGAACTATGCTGAATCCTTGCTGCATCGATTCGGAATACTTGACGTTGATGGTGGTCCTGGGAAGGGGTTATCTGAAGAGTTGGAGAATTTACAAGCAGGCCCACTTTCAAAACTTTTCAAACCATCGCCTCTCATTGTTGATTCTAAAGAGGATGAAAGTTCAGGAGATGGGAAAGGGGGCAAGGCTTTGCACTTGCCAGATGATGTGGACGTGTCCGTAGAATTGAAAAATTGGTTATTTGCTCTTGAAGGTGCACACGAAATTGCAGTGTTTAGGAGTTCCTACGATCAGGAGGGTGTGCGCAGAGAAGAGAGGTCTTGGCACACAACATTCCATAATCTGCACCTAAAAGGAAAAAGTAGTCCAAAACAAATGATGGATGGTATTAGGAAGTCATACAGGACACCAAAATTTCCTATAGAATTGGTGACGGTTGGTGTGGAAGGCTTGCAGATCTTAAAGCCACATGCCCAGAACTACAATAATCCTGCTGTCGTACACATGAATGGGATCAAGGAATCTGCAGGTGTAAATCTTGAAATTCGCCTGGTGACGATGGAGGACAGTGTTGATCATGAAATGGTTGAATGGGTTGTAGAAAATGTGAAATTCTCTGTTGAGCAACCGATTGAGGCGGTTGTATCAAAGGATGAGCTACAACATCTTGTTGTTCTGTGCAAGTCAGAAGTTGACTCAATGGGTCGAATAACGGCTGGAATTCTGCAGCTATTTAAGTTGGAGGAGACCATAGGCCAGGCTGCTATGAATCAACTGACCAACCTTGGAAGTGAGGGCTTCAACAAGATCTTCTCCCCTGAGAAGCTCGGCAGAGGTAGCAGCTTCGGCAGTGTTGGGTTCCCTCAATCAAATTTGATAAATGAATGCCCAATTACAAGTACAACCACATCAGAAATGACTGTAGCTTCGCTTGAGGAGGTCGTTATAGATTCACAACTTAAGTGTGCTGCTCTTCTTACCGAACTGAGTAGTTCAGATTCTTCCATGCAACATCTTGCAAGTGTAAAACAACTCACTCAAAAACTTCAGAGTATGCAGAGTTTATTGACACAACTAAAATCTCAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chorein_N

Protein Analysis

1207

Amino Acids

131.92

Weight (kDa)

5.36

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLTP3A_B PF24917 19 - 204 1.4e-12 Bridge-like lipid transfer protein family member 3A/B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2189
AarI CACCTGC 1 cut(s) 3047
Acc16I TGCGCA 1 cut(s) 2834
Acc36I ACCTGC 1 cut(s) 3047
AccII CGCG 2 cut(s) 21, 23
AciI CCGC 5 cut(s) 152, 324, 700, 928, 3167
AclWI GGATC 8 cut(s) 1371, 1384, 1397, 1640, 1653, 1968, 2424, 3050
AcoI YGGCCR 1 cut(s) 174
AcuI CTGAAG 5 cut(s) 1352, 1748, 2093, 2625, 3560
AfaI GTAC 7 cut(s) 32, 44, 658, 1973, 2017, 3030, 3430
AfeI AGCGCT 1 cut(s) 722
AfiI CCNNNNNNNGG 8 cut(s) 442, 561, 1199, 1386, 1880, 1883, 2049, 2594
AflII CTTAAG 2 cut(s) 530, 3488
AflIII ACRYGT 1 cut(s) 2742
AjiI CACGTC 3 cut(s) 166, 1507, 2743
AjnI CCWGG 7 cut(s) 844, 1401, 1426, 1879, 2587, 3078, 3285
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 548, 580
AleI CACNNNNGTG 1 cut(s) 1145
Alw21I GWGCWC 3 cut(s) 859, 1240, 2788
Alw26I GTCTC 3 cut(s) 60, 1667, 3269
Alw44I GTGCAC 1 cut(s) 2784
AlwI GGATC 8 cut(s) 1371, 1384, 1397, 1640, 1653, 1968, 2424, 3050
AlwNI CAGNNNCTG 1 cut(s) 2225
Ama87I CYCGRG 1 cut(s) 63
Aor51HI AGCGCT 1 cut(s) 722
AoxI GGCC 4 cut(s) 174, 946, 2629, 3283
ApaLI GTGCAC 1 cut(s) 2784
ArsI GACNNNNNNTTYG 4 cut(s) 2349, 2381, 3439, 3471
AseI ATTAAT 1 cut(s) 1341
Asp700I GAANNNNTTC 1 cut(s) 1592
AspA2I CCTAGG 1 cut(s) 2518
AspLEI GCGC 5 cut(s) 23, 25, 160, 723, 2835
AspS9I GGNCC 4 cut(s) 973, 1782, 2585, 2630
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 6 cut(s) 160, 614, 1599, 2110, 2971, 3094
AsuNHI GCTAGC 1 cut(s) 1688
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 3 cut(s) 973, 1782, 2585
AvrII CCTAGG 1 cut(s) 2518
AxyI CCTNAGG 1 cut(s) 1786
BaeGI GKGCMC 1 cut(s) 2788
BaeI ACNNNNGTAYC 2 cut(s) 2070, 2103
BamHI GGATCC 2 cut(s) 1376, 1645
BanII GRGCYC 2 cut(s) 1240, 1859
BauI CACGAG 1 cut(s) 420
Bbv12I GWGCWC 3 cut(s) 859, 1240, 2788
BceAI ACGGC 5 cut(s) 136, 189, 301, 933, 3258
BcgI CGANNNNNNTGC 6 cut(s) 223, 257, 272, 306, 438, 472
BciT130I CCWGG 7 cut(s) 846, 1403, 1428, 1881, 2589, 3080, 3287
BciVI GTATCC 3 cut(s) 1111, 2355, 2364
BclI TGATCA 1 cut(s) 3103
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 60, 1667, 3269
BfaI CTAG 8 cut(s) 110, 138, 1689, 1710, 1736, 2019, 2159, 2519
BfmI CTRYAG 6 cut(s) 80, 384, 2949, 3054, 3254, 3450
BfoI RGCGCY 2 cut(s) 161, 724
BfrI CTTAAG 2 cut(s) 530, 3488
BfuAI ACCTGC 1 cut(s) 3047
BfuI GTATCC 3 cut(s) 1111, 2355, 2364
BglII AGATCT 2 cut(s) 2985, 3336
BlnI CCTAGG 1 cut(s) 2518
BlpI GCTNAGC 1 cut(s) 1239
BmcAI AGTACT 2 cut(s) 44, 2017
Bme1390I CCNGG 8 cut(s) 334, 846, 1403, 1428, 1881, 2589, 3080, 3287
Bme18I GGWCC 3 cut(s) 973, 1782, 2585
BmeT110I CYCGRG 1 cut(s) 63
BmgBI CACGTC 3 cut(s) 166, 1507, 2743
BmgT120I GGNCC 4 cut(s) 973, 1782, 2585, 2630
BmiI GGNNCC 9 cut(s) 231, 485, 1179, 1378, 1647, 1783, 1856, 2070, 3389
BmrFI CCNGG 8 cut(s) 334, 846, 1403, 1428, 1881, 2589, 3080, 3287
BmtI GCTAGC 1 cut(s) 1692
BpmI CTGGAG 3 cut(s) 47, 1299, 1587
Bpu10I CCTNAGC 1 cut(s) 1308
Bpu1102I GCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 6 cut(s) 23, 608, 815, 921, 1460, 3482
BpuMI CCSGG 1 cut(s) 334
Bsa29I ATCGAT 3 cut(s) 609, 2419, 2557
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 1469
BsaI GGTCTC 2 cut(s) 60, 3269
BsaJI CCNNGG 5 cut(s) 171, 436, 2518, 2588, 3313
BsaWI WCCGGW 2 cut(s) 864, 1441
Bsc4I CCNNNNNNNGG 8 cut(s) 442, 561, 1199, 1386, 1880, 1883, 2049, 2594
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 5 cut(s) 50, 70, 880, 1422, 1679
Bse21I CCTNAGG 1 cut(s) 1786
Bse3DI GCAATG 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 1469
BseBI CCWGG 7 cut(s) 846, 1403, 1428, 1881, 2589, 3080, 3287
BseCI ATCGAT 3 cut(s) 609, 2419, 2557
BseDI CCNNGG 5 cut(s) 171, 436, 2518, 2588, 3313
BseGI GGATG 7 cut(s) 295, 646, 1470, 2041, 2689, 2917, 3187
BseJI GATNNNNATC 1 cut(s) 1469
BseLI CCNNNNNNNGG 8 cut(s) 442, 561, 1199, 1386, 1880, 1883, 2049, 2594
BseMI GCAATG 1 cut(s) 223
BseNI ACTGG 5 cut(s) 50, 70, 880, 1422, 1679
BsePI GCGCGC 1 cut(s) 21
BseRI GAGGAG 5 cut(s) 287, 915, 1866, 3287, 3479
BseSI GKGCMC 1 cut(s) 2788
BsgI GTGCAG 5 cut(s) 116, 146, 263, 2238, 2858
Bsh1236I CGCG 2 cut(s) 21, 23
BshFI GGCC 4 cut(s) 176, 948, 2631, 3285
BshVI ATCGAT 3 cut(s) 609, 2419, 2557
BsiHKAI GWGCWC 3 cut(s) 859, 1240, 2788
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 4 cut(s) 155, 333, 865, 1442
BslFI GGGAC 4 cut(s) 99, 1250, 1717, 1795
BslI CCNNNNNNNGG 8 cut(s) 442, 561, 1199, 1386, 1880, 1883, 2049, 2594
BsmAI GTCTC 3 cut(s) 60, 1667, 3269
BsmFI GGGAC 4 cut(s) 99, 1250, 1717, 1795
BsmI GAATGC 4 cut(s) 283, 2157, 2511, 3419
BsnI GGCC 4 cut(s) 176, 948, 2631, 3285
Bso31I GGTCTC 2 cut(s) 60, 3269
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 4 cut(s) 859, 1240, 1859, 2788
Bsp1720I GCTNAGC 1 cut(s) 1239
BspACI CCGC 5 cut(s) 152, 324, 700, 928, 3167
BspANI GGCC 4 cut(s) 176, 948, 2631, 3285
BspDI ATCGAT 3 cut(s) 609, 2419, 2557
BspFNI CGCG 2 cut(s) 21, 23
BspHI TCATGA 1 cut(s) 3106
BspLI GGNNCC 9 cut(s) 231, 485, 1179, 1378, 1647, 1783, 1856, 2070, 3389
BspMAI CTGCAG 3 cut(s) 84, 3058, 3258
BspMI ACCTGC 1 cut(s) 3047
BspOI GCTAGC 1 cut(s) 1692
BspPI GGATC 8 cut(s) 1371, 1384, 1397, 1640, 1653, 1968, 2424, 3050
BspQI GCTCTTC 2 cut(s) 864, 3507
BspTI CTTAAG 2 cut(s) 530, 3488
BspTNI GGTCTC 2 cut(s) 60, 3269
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 5 cut(s) 50, 70, 880, 1422, 1679
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 5 cut(s) 171, 436, 2518, 2588, 3313
BssHII GCGCGC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 3 cut(s) 436, 2518, 3313
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 7 cut(s) 846, 1403, 1428, 1881, 2589, 3080, 3287
Bst4CI ACNGT 9 cut(s) 94, 400, 760, 1336, 1495, 1944, 2965, 3098, 3451
Bst6I CTCTTC 7 cut(s) 864, 1040, 1293, 1399, 2601, 2835, 3507
BstAFI CTTAAG 2 cut(s) 530, 3488
BstBAI YACGTR 1 cut(s) 854
BstDSI CCRYGG 1 cut(s) 171
BstENI CCTNNNNNAGG 1 cut(s) 1384
BstF5I GGATG 7 cut(s) 295, 646, 1470, 2041, 2689, 2917, 3187
BstFNI CGCG 2 cut(s) 21, 23
BstH2I RGCGCY 2 cut(s) 161, 724
BstHHI GCGC 5 cut(s) 23, 25, 160, 723, 2835
BstMAI GTCTC 3 cut(s) 60, 1667, 3269
BstMWI GCNNNNNNNGC 8 cut(s) 157, 160, 306, 452, 650, 701, 3366, 3375
BstNI CCWGG 7 cut(s) 846, 1403, 1428, 1881, 2589, 3080, 3287
BstNSI RCATGY 4 cut(s) 1166, 1190, 1746, 3002
BstSCI CCNGG 8 cut(s) 332, 844, 1401, 1426, 1879, 2587, 3078, 3285
BstSFI CTRYAG 6 cut(s) 80, 384, 2949, 3054, 3254, 3450
BstSLI GKGCMC 1 cut(s) 2788
BstUI CGCG 2 cut(s) 21, 23
BstX2I RGATCY 6 cut(s) 1376, 1389, 1645, 1960, 2985, 3336
BstYI RGATCY 6 cut(s) 1376, 1389, 1645, 1960, 2985, 3336
Bsu15I ATCGAT 3 cut(s) 609, 2419, 2557
Bsu36I CCTNAGG 1 cut(s) 1786
BsuI GTATCC 3 cut(s) 1111, 2355, 2364
BsuRI GGCC 4 cut(s) 176, 948, 2631, 3285
BsuTUI ATCGAT 3 cut(s) 609, 2419, 2557
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 3 cut(s) 138, 441, 2640
BtrI CACGTC 3 cut(s) 166, 1507, 2743
BtsCI GGATG 7 cut(s) 295, 646, 1470, 2041, 2689, 2917, 3187
BtsI GCAGTG 4 cut(s) 1250, 1746, 2805, 3385
BtsIMutI CAGTG 7 cut(s) 90, 765, 1250, 1746, 2805, 3103, 3385
BveI ACCTGC 1 cut(s) 3047
CaiI CAGNNNCTG 1 cut(s) 2225
CciI TCATGA 1 cut(s) 3106
CfoI GCGC 5 cut(s) 23, 25, 160, 723, 2835
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 4 cut(s) 973, 1782, 2585, 2630
ClaI ATCGAT 3 cut(s) 609, 2419, 2557
CseI GACGC 1 cut(s) 96
Csp6I GTAC 7 cut(s) 31, 43, 657, 1972, 2016, 3029, 3429
CspCI CAANNNNNGTGG 2 cut(s) 951, 986
CviQI GTAC 7 cut(s) 31, 43, 657, 1972, 2016, 3029, 3429
DraI TTTAAA 1 cut(s) 2457
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 7 cut(s) 864, 1040, 1293, 1399, 2601, 2835, 3507
EarI CTCTTC 7 cut(s) 864, 1040, 1293, 1399, 2601, 2835, 3507
Ecl136II GAGCTC 1 cut(s) 1238
Eco130I CCWWGG 3 cut(s) 436, 2518, 3313
Eco24I GRGCYC 2 cut(s) 1240, 1859
Eco31I GGTCTC 2 cut(s) 60, 3269
Eco47I GGWCC 3 cut(s) 973, 1782, 2585
Eco47III AGCGCT 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 1238
Eco57I CTGAAG 5 cut(s) 1352, 1748, 2093, 2625, 3560
Eco81I CCTNAGG 1 cut(s) 1786
Eco88I CYCGRG 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 1384
EcoO109I RGGNCCY 1 cut(s) 1782
EcoRI GAATTC 3 cut(s) 1004, 1413, 3249
EcoRII CCWGG 7 cut(s) 844, 1401, 1426, 1879, 2587, 3078, 3285
EcoT14I CCWWGG 3 cut(s) 436, 2518, 3313
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 2 cut(s) 1240, 1859
ErhI CCWWGG 3 cut(s) 436, 2518, 3313
FalI AAGNNNNNCTT 2 cut(s) 1120, 1152
FaqI GGGAC 4 cut(s) 99, 1250, 1717, 1795
FauI CCCGC 1 cut(s) 693
FbaI TGATCA 1 cut(s) 3103
FokI GGATG 7 cut(s) 302, 653, 1457, 2048, 2696, 2924, 3194
FriOI GRGCYC 2 cut(s) 1240, 1859
FspBI CTAG 8 cut(s) 110, 138, 1689, 1710, 1736, 2019, 2159, 2519
FspI TGCGCA 1 cut(s) 2834
GlaI GCGC 5 cut(s) 22, 24, 159, 722, 2834
GsuI CTGGAG 3 cut(s) 47, 1299, 1587
HaeII RGCGCY 2 cut(s) 161, 724
HaeIII GGCC 4 cut(s) 176, 948, 2631, 3285
HapII CCGG 4 cut(s) 155, 333, 865, 1442
HgaI GACGC 1 cut(s) 96
HhaI GCGC 5 cut(s) 23, 25, 160, 723, 2835
Hin6I GCGC 5 cut(s) 21, 23, 158, 721, 2833
HinP1I GCGC 5 cut(s) 21, 23, 158, 721, 2833
HincII GTYRAC 3 cut(s) 933, 1676, 3223
HindII GTYRAC 3 cut(s) 933, 1676, 3223
HindIII AAGCTT 2 cut(s) 747, 1010
HpaII CCGG 4 cut(s) 155, 333, 865, 1442
HphI GGTGA 6 cut(s) 160, 614, 1599, 2110, 2971, 3094
Hpy99I CGWCG 1 cut(s) 322
HpyCH4III ACNGT 9 cut(s) 94, 400, 760, 1336, 1495, 1944, 2965, 3098, 3451
HpyCH4IV ACGT 6 cut(s) 165, 853, 1506, 2242, 2574, 2742
HpyF10VI GCNNNNNNNGC 8 cut(s) 157, 160, 306, 452, 650, 701, 3366, 3375
HpySE526I ACGT 6 cut(s) 165, 853, 1506, 2242, 2574, 2742
HspAI GCGC 5 cut(s) 21, 23, 158, 721, 2833
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 3103
LguI GCTCTTC 2 cut(s) 864, 3507
LmnI GCTCC 5 cut(s) 718, 1235, 1539, 1568, 1854
MaeI CTAG 8 cut(s) 110, 138, 1689, 1710, 1736, 2019, 2159, 2519
MaeII ACGT 6 cut(s) 165, 853, 1506, 2242, 2574, 2742
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 6 cut(s) 1376, 1389, 1645, 1960, 2985, 3336
MhlI GDGCHC 4 cut(s) 859, 1240, 1859, 2788
MlyI GAGTC 7 cut(s) 14, 189, 904, 1098, 1613, 2202, 3218
MmeI TCCRAC 6 cut(s) 163, 249, 267, 1987, 2592, 3249
Mph1103I ATGCAT 1 cut(s) 1164
MreI CGCCGGCG 1 cut(s) 154
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 1592
MslI CAYNNNNRTG 3 cut(s) 1145, 2435, 3036
MspA1I CMGCKG 2 cut(s) 928, 1757
MspCI CTTAAG 2 cut(s) 530, 3488
MspI CCGG 4 cut(s) 155, 333, 865, 1442
MspR9I CCNGG 8 cut(s) 334, 846, 1403, 1428, 1881, 2589, 3080, 3287
MunI CAATTG 1 cut(s) 963
Mva1269I GAATGC 4 cut(s) 283, 2157, 2511, 3419
MvaI CCWGG 7 cut(s) 846, 1403, 1428, 1881, 2589, 3080, 3287
MvnI CGCG 2 cut(s) 21, 23
MwoI GCNNNNNNNGC 8 cut(s) 157, 160, 306, 452, 650, 701, 3366, 3375
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 334
NgoMIV GCCGGC 1 cut(s) 154
NheI GCTAGC 1 cut(s) 1688
NlaIV GGNNCC 9 cut(s) 231, 485, 1179, 1378, 1647, 1783, 1856, 2070, 3389
NmeAIII GCCGAG 2 cut(s) 307, 3336
NmuCI GTSAC 5 cut(s) 166, 1605, 2194, 2959, 3082
NsbI TGCGCA 1 cut(s) 2834
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 4 cut(s) 1166, 1190, 1746, 3002
OliI CACNNNNGTG 1 cut(s) 1145
PaeI GCATGC 1 cut(s) 1166
PagI TCATGA 1 cut(s) 3106
PaqCI CACCTGC 1 cut(s) 3047
PauI GCGCGC 1 cut(s) 21
PciSI GCTCTTC 2 cut(s) 864, 3507
PctI GAATGC 4 cut(s) 283, 2157, 2511, 3419
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 1592
PfoI TCCNGGA 1 cut(s) 332
PleI GAGTC 7 cut(s) 13, 188, 903, 1098, 1612, 2201, 3218
PpsI GAGTC 7 cut(s) 13, 188, 903, 1098, 1612, 2201, 3218
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 1782
PshBI ATTAAT 1 cut(s) 1341
PsiI TTATAA 1 cut(s) 2189
Psp124BI GAGCTC 1 cut(s) 1240
Psp5II RGGWCCY 1 cut(s) 1782
Psp6I CCWGG 7 cut(s) 844, 1401, 1426, 1879, 2587, 3078, 3285
PspGI CCWGG 7 cut(s) 844, 1401, 1426, 1879, 2587, 3078, 3285
PspN4I GGNNCC 9 cut(s) 231, 485, 1179, 1378, 1647, 1783, 1856, 2070, 3389
PspPI GGNCC 4 cut(s) 973, 1782, 2585, 2630
PspPPI RGGWCCY 1 cut(s) 1782
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstI CTGCAG 3 cut(s) 84, 3058, 3258
PstNI CAGNNNCTG 1 cut(s) 2225
PsuI RGATCY 6 cut(s) 1376, 1389, 1645, 1960, 2985, 3336
PteI GCGCGC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 1757
RsaI GTAC 7 cut(s) 32, 44, 658, 1973, 2017, 3030, 3430
RsaNI GTAC 7 cut(s) 31, 43, 657, 1972, 2016, 3029, 3429
RseI CAYNNNNRTG 3 cut(s) 1145, 2435, 3036
SacI GAGCTC 1 cut(s) 1240
SapI GCTCTTC 2 cut(s) 864, 3507
Sau96I GGNCC 4 cut(s) 973, 1782, 2585, 2630
ScaI AGTACT 2 cut(s) 44, 2017
SchI GAGTC 7 cut(s) 14, 189, 904, 1098, 1613, 2202, 3218
ScrFI CCNGG 8 cut(s) 334, 846, 1403, 1428, 1881, 2589, 3080, 3287
SduI GDGCHC 4 cut(s) 859, 1240, 1859, 2788
SfcI CTRYAG 6 cut(s) 80, 384, 2949, 3054, 3254, 3450
SgrAI CRCCGGYG 1 cut(s) 154
SinI GGWCC 3 cut(s) 973, 1782, 2585
SmiMI CAYNNNNRTG 3 cut(s) 1145, 2435, 3036
SmlI CTYRAG 8 cut(s) 38, 530, 587, 794, 936, 1475, 3461, 3488
SmoI CTYRAG 8 cut(s) 38, 530, 587, 794, 936, 1475, 3461, 3488
SphI GCATGC 1 cut(s) 1166
SsiI CCGC 5 cut(s) 152, 324, 700, 928, 3167
SspI AATATT 1 cut(s) 406
SspMI CTAG 8 cut(s) 110, 138, 1689, 1710, 1736, 2019, 2159, 2519
SstI GAGCTC 1 cut(s) 1240
StyD4I CCNGG 8 cut(s) 332, 844, 1401, 1426, 1879, 2587, 3078, 3285
StyI CCWWGG 3 cut(s) 436, 2518, 3313
TaaI ACNGT 9 cut(s) 94, 400, 760, 1336, 1495, 1944, 2965, 3098, 3451
TaiI ACGT 6 cut(s) 168, 856, 1509, 2245, 2577, 2745
TaqI TCGA 8 cut(s) 258, 609, 898, 1085, 1257, 2419, 2557, 3235
TatI WGTACW 5 cut(s) 30, 42, 656, 2015, 3428
TauI GCSGC 1 cut(s) 154
TscAI CASTG 7 cut(s) 97, 765, 1250, 1753, 2805, 3103, 3385
TseFI GTSAC 5 cut(s) 166, 1605, 2194, 2959, 3082
Tsp45I GTSAC 5 cut(s) 166, 1605, 2194, 2959, 3082
TspGWI ACGGA 4 cut(s) 496, 653, 830, 2737
TspRI CASTG 7 cut(s) 97, 765, 1250, 1753, 2805, 3103, 3385
Vha464I CTTAAG 2 cut(s) 530, 3488
VneI GTGCAC 1 cut(s) 2784
VpaK11BI GGWCC 3 cut(s) 973, 1782, 2585
VspI ATTAAT 1 cut(s) 1341
XagI CCTNNNNNAGG 1 cut(s) 1384
XbaI TCTAGA 1 cut(s) 109
XceI RCATGY 4 cut(s) 1166, 1190, 1746, 3002
XcmI CCANNNNNNNNNTGG 3 cut(s) 112, 1228, 2396
XmaJI CCTAGG 1 cut(s) 2518
XmnI GAANNNNTTC 1 cut(s) 1592
XspI CTAG 8 cut(s) 110, 138, 1689, 1710, 1736, 2019, 2159, 2519
ZrmI AGTACT 2 cut(s) 44, 2017
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.