AT3G20740

fie, fis3, fie1 fie (fertilization-independent endosperm)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009336
Physical Location & Seq
Reverse (-)
7248809 .. 7252452
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20740.1

Sequence Viewer

Length: 1110 bp
ATGTCGAAGATAACCTTAGGGAACGAGTCAATAGTTGGGTCTTTGACTCCATCGAATAAGAAATCGTACAAAGTGACGAATAGGATTCAGGAAGGGAAGAAACCTTTGTATGCTGTTGTTTTCAACTTCCTTGATGCTCGTTTCTTCGATGTCTTCGTTACCGCTGGTGGAAATCGGATTACTCTGTACAATTGTCTCGGAGATGGTGCCATATCAGCATTGCAATCCTATGCTGATGAAGATAAGGAAGAGTCGTTTTACACGGTAAGTTGGGCGTGTGGCGTTAATGGGAACCCATATGTTGCGGCTGGAGGAGTAAAAGGTATAATCCGAGTCATTGACGTCAACAGTGAAACGATTCATAAGAGTCTTGTGGGTCATGGAGATTCAGTGAACGAAATCAGGACACAACCTTTAAAACCTCAACTTGTGATTACTGCTAGCAAGGATGAATCTGTTCGTTTGTGGAATGTTGAAACTGGGATATGTATTTTGATATTTGCTGGAGCTGGAGGTCATCGCTATGAAGTTCTAAGTGTGGATTTTCATCCGTCTGATATTTACCGCTTTGCTAGTTGTGGTATGGACACCACTATTAAAATATGGTCAATGAAAGAGTTTTGGACGTACGTCGAGAAGTCATTCACATGGACTGATGATCCATCAAAATTCCCCACAAAATTTGTCCAATTCCCTGTATTTACAGCTTCCATTCATACAAATTATGTAGATTGTAACCGTTGGTTTGGTGATTTTATCCTCTCAAAGAGTGTGGACAACGAGATCCTGTTGTGGGAACCACAACTGAAAGAGAATTCTCCTGGCGAGGGAGCTTCAGATGTTCTATTAAGATACCCGGTTCCAATGTGTGATATTTGGTTTATCAAGTTTTCTTGTGACCTCCATTTAAGTTCTGTTGCGATAGGTAATCAGGAAGGAAAGGTTTATGTCTGGGATTTGAAAAGTTGCCCTCCTGTTTTGATTACAAAGTTATCACACAATCAATCAAAGTCTGTAATCAGGCAAACAGCCATGTCTGTCGATGGAAGCACGATTCTTGCTTGCTGCGAGGACGGGACTATATGGCGCTGGGACGTGATTACCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009409 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009960 GO:0009987 GO:0010154 GO:0010162 GO:0010231 GO:0010431 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090567 GO:0090568 GO:0090696 GO:0097437 GO:0099402 GO:0140110 GO:1901360 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

369

Amino Acids

41.26

Weight (kDa)

5.85

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 51 - 207 9.3e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 85 - 164 6.6e-12 THOC3 beta-propeller domain
WD40_Gbeta PF25391 87 - 206 1.9e-07 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 98 - 210 7.5e-15 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 100 - 206 3.1e-15 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 100 - 164 4.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 101 - 203 3.1e-14 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 211 1.3e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 142 - 207 4.2e-06 WDR3 second beta-propeller domain
WD40 PF00400 172 - 203 3.9e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
AccB1I GGYRCC 1 cut(s) 206
AciI CCGC 3 cut(s) 162, 305, 565
AclWI GGATC 2 cut(s) 653, 778
AcsI RAATTY 3 cut(s) 668, 680, 814
AcuI CTGAAG 1 cut(s) 819
AcyI GRCGYC 1 cut(s) 342
AfaI GTAC 3 cut(s) 68, 188, 629
AfiI CCNNNNNNNGG 2 cut(s) 793, 827
AgsI TTSAA 3 cut(s) 124, 476, 961
AjiI CACGTC 1 cut(s) 1096
AjnI CCWGG 1 cut(s) 820
AluBI AGCT 3 cut(s) 509, 707, 833
AluI AGCT 3 cut(s) 509, 707, 833
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 2 cut(s) 653, 778
ApeKI GCWGC 1 cut(s) 1065
ApoI RAATTY 3 cut(s) 668, 680, 814
Asp700I GAANNNNTTC 3 cut(s) 357, 456, 641
AspLEI GCGC 1 cut(s) 1089
AsuC2I CCSGG 1 cut(s) 857
AsuHPI GGTGA 1 cut(s) 761
AsuNHI GCTAGC 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 16
BanI GGYRCC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 1 cut(s) 1052
BccI CCATC 4 cut(s) 58, 197, 670, 1037
BciT130I CCWGG 1 cut(s) 822
BcnI CCSGG 1 cut(s) 857
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 2 cut(s) 441, 573
BfoI RGCGCY 1 cut(s) 1090
BisI GCNGC 2 cut(s) 306, 1066
BlsI GCNGC 2 cut(s) 307, 1067
Bme1390I CCNGG 2 cut(s) 822, 857
BmgBI CACGTC 1 cut(s) 1096
BmiI GGNNCC 4 cut(s) 208, 293, 798, 861
BmrFI CCNGG 2 cut(s) 822, 857
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 124
BmtI GCTAGC 1 cut(s) 444
BmuI ACTGGG 1 cut(s) 489
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 3 cut(s) 330, 525, 531
BpuMI CCSGG 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 342
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 2 cut(s) 793, 827
Bse1I ACTGG 1 cut(s) 484
Bse21I CCTNAGG 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 218
Bse8I GATNNNNATC 1 cut(s) 546
BseBI CCWGG 1 cut(s) 822
BseGI GGATG 2 cut(s) 454, 547
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 2 cut(s) 793, 827
BseMI GCAATG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 484
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 1 cut(s) 1052
BseYI CCCAGC 1 cut(s) 1089
BshNI GGYRCC 1 cut(s) 206
BsiSI CCGG 1 cut(s) 857
BsiWI CGTACG 1 cut(s) 627
BslFI GGGAC 2 cut(s) 1090, 1106
BslI CCNNNNNNNGG 2 cut(s) 793, 827
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 1090, 1106
Bsp1407I TGTACA 1 cut(s) 186
Bsp143I GATC 2 cut(s) 658, 783
BspACI CCGC 3 cut(s) 162, 305, 565
BspLI GGNNCC 4 cut(s) 208, 293, 798, 861
BspOI GCTAGC 1 cut(s) 444
BspPI GGATC 2 cut(s) 653, 778
BspT107I GGYRCC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 186
BsrI ACTGG 1 cut(s) 484
BssMI GATC 2 cut(s) 658, 783
BssNI GRCGYC 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 822
Bst4CI ACNGT 3 cut(s) 265, 350, 740
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 442, 1063
BstDEI CTNAG 2 cut(s) 16, 533
BstF5I GGATG 2 cut(s) 454, 547
BstH2I RGCGCY 1 cut(s) 1090
BstHHI GCGC 1 cut(s) 1089
BstKTI GATC 2 cut(s) 661, 786
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 2 cut(s) 658, 783
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 822
BstPAI GACNNNNGTC 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 820, 855
BstV1I GCAGC 1 cut(s) 1052
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 783
BstYI RGATCY 1 cut(s) 783
Bsu36I CCTNAGG 1 cut(s) 16
BtgZI GCGATG 1 cut(s) 503
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 2 cut(s) 454, 547
BtsIMutI CAGTG 2 cut(s) 355, 396
Cac8I GCNNGC 2 cut(s) 442, 1063
CfoI GCGC 1 cut(s) 1089
Csp6I GTAC 3 cut(s) 67, 187, 628
CspCI CAANNNNNGTGG 4 cut(s) 664, 699, 753, 788
CviAII CATG 3 cut(s) 380, 648, 1033
CviJI RGCY 5 cut(s) 308, 509, 707, 833, 1031
CviKI_1 RGCY 5 cut(s) 308, 509, 707, 833, 1031
CviQI GTAC 3 cut(s) 67, 187, 628
DdeI CTNAG 2 cut(s) 16, 533
DpnI GATC 2 cut(s) 660, 785
DpnII GATC 2 cut(s) 658, 783
DraI TTTAAA 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 819
Eco81I CCTNAGG 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 820
FaeI CATG 3 cut(s) 383, 651, 1036
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 2 cut(s) 1090, 1106
FatI CATG 3 cut(s) 379, 647, 1032
FauNDI CATATG 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 306, 1066
FokI GGATG 2 cut(s) 461, 534
Fsp4HI GCNGC 2 cut(s) 306, 1066
FspBI CTAG 2 cut(s) 441, 573
GlaI GCGC 1 cut(s) 1088
GluI GCNGC 2 cut(s) 306, 1066
GsaI CCCAGC 1 cut(s) 1093
GsuI CTGGAG 3 cut(s) 330, 525, 531
HaeII RGCGCY 1 cut(s) 1090
HapII CCGG 1 cut(s) 857
HhaI GCGC 1 cut(s) 1089
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 3 cut(s) 383, 651, 1036
Hin6I GCGC 1 cut(s) 1087
HinP1I GCGC 1 cut(s) 1087
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HpaII CCGG 1 cut(s) 857
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 3 cut(s) 346, 394, 775
Hpy188I TCNGA 5 cut(s) 177, 200, 332, 556, 838
Hpy188III TCNNGA 4 cut(s) 89, 403, 634, 932
Hpy8I GTNNAC 3 cut(s) 346, 394, 775
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 86, 929
HpyCH4III ACNGT 3 cut(s) 265, 350, 740
HpyCH4IV ACGT 4 cut(s) 342, 626, 630, 1095
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 2 cut(s) 16, 533
HpySE526I ACGT 4 cut(s) 342, 626, 630, 1095
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 3 cut(s) 383, 651, 1036
HspAI GCGC 1 cut(s) 1087
Kzo9I GATC 2 cut(s) 658, 783
LmnI GCTCC 2 cut(s) 506, 830
Lsp1109I GCAGC 1 cut(s) 1052
LweI GCATC 1 cut(s) 124
MaeI CTAG 2 cut(s) 441, 573
MaeII ACGT 4 cut(s) 342, 626, 630, 1095
MaeIII GTNAC 4 cut(s) 73, 157, 734, 896
MalI GATC 2 cut(s) 660, 785
MboI GATC 2 cut(s) 658, 783
MboII GAAGA 6 cut(s) 19, 109, 136, 145, 251, 260
MfeI CAATTG 1 cut(s) 190
MflI RGATCY 1 cut(s) 783
MluCI AATT 6 cut(s) 190, 668, 680, 689, 721, 814
MlyI GAGTC 5 cut(s) 35, 40, 260, 342, 376
MnlI CCTC 8 cut(s) 305, 432, 506, 770, 820, 911, 981, 1063
MroXI GAANNNNTTC 3 cut(s) 357, 456, 641
MseI TTAA 5 cut(s) 285, 416, 597, 848, 908
MslI CAYNNNNRTG 2 cut(s) 522, 646
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 857
MspR9I CCNGG 2 cut(s) 822, 857
MunI CAATTG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 822
MwoI GCNNNNNNNGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 857
NdeI CATATG 1 cut(s) 298
NdeII GATC 2 cut(s) 658, 783
NheI GCTAGC 1 cut(s) 440
NlaIII CATG 3 cut(s) 383, 651, 1036
NlaIV GGNNCC 4 cut(s) 208, 293, 798, 861
NmuCI GTSAC 2 cut(s) 73, 896
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 3 cut(s) 357, 456, 641
PfeI GAWTC 5 cut(s) 85, 358, 386, 452, 1054
Pfl23II CGTACG 1 cut(s) 627
PkrI GCNGC 2 cut(s) 307, 1067
PleI GAGTC 5 cut(s) 34, 40, 259, 341, 375
PpsI GAGTC 5 cut(s) 34, 40, 259, 341, 375
PshAI GACNNNNGTC 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 820
PspFI CCCAGC 1 cut(s) 1089
PspGI CCWGG 1 cut(s) 820
PspLI CGTACG 1 cut(s) 627
PspN4I GGNNCC 4 cut(s) 208, 293, 798, 861
PsuI RGATCY 1 cut(s) 783
RsaI GTAC 3 cut(s) 68, 188, 629
RsaNI GTAC 3 cut(s) 67, 187, 628
RseI CAYNNNNRTG 2 cut(s) 522, 646
SaqAI TTAA 5 cut(s) 285, 416, 597, 848, 908
SatI GCNGC 2 cut(s) 306, 1066
Sau3AI GATC 2 cut(s) 658, 783
SchI GAGTC 5 cut(s) 35, 40, 260, 342, 376
ScrFI CCNGG 2 cut(s) 822, 857
SfaNI GCATC 1 cut(s) 124
SmiMI CAYNNNNRTG 2 cut(s) 522, 646
Sse9I AATT 6 cut(s) 190, 668, 680, 689, 721, 814
SsiI CCGC 3 cut(s) 162, 305, 565
SspMI CTAG 2 cut(s) 441, 573
StyD4I CCNGG 2 cut(s) 820, 855
TaaI ACNGT 3 cut(s) 265, 350, 740
TaiI ACGT 4 cut(s) 345, 629, 633, 1098
TaqI TCGA 5 cut(s) 5, 53, 147, 633, 1041
TasI AATT 6 cut(s) 190, 668, 680, 689, 721, 814
TatI WGTACW 1 cut(s) 186
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 5 cut(s) 85, 358, 386, 452, 1054
Tru1I TTAA 5 cut(s) 285, 416, 597, 848, 908
Tru9I TTAA 5 cut(s) 285, 416, 597, 848, 908
TscAI CASTG 2 cut(s) 355, 396
TseFI GTSAC 2 cut(s) 73, 896
TseI GCWGC 1 cut(s) 1065
Tsp45I GTSAC 2 cut(s) 73, 896
TspDTI ATGAA 7 cut(s) 252, 350, 465, 536, 540, 626, 704
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 2 cut(s) 355, 396
XapI RAATTY 3 cut(s) 668, 680, 814
XmnI GAANNNNTTC 3 cut(s) 357, 456, 641
XspI CTAG 2 cut(s) 441, 573
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.