Rw5G023540

Polycomb group protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009336
Physical Location & Seq
Reverse (-)
33206892 .. 33210645
3754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023540.1

Sequence Viewer

Length: 1113 bp
ATGGCCAAGTTCGCTTTAGGATCGGAACCGGTGGTGGGCTCCCTCTCATCCTCCAAGAAGAGAGAGTACAGAGTCACCAACCGCCTCCAAGAGGGCAAGAAACCCATATACGCCGTCGTTTTCAACTTCATCGACTCTCGCTACTTCAACGTCTTCGCCACCGTCGGCGGCAACCGGGTGACTGTGTATGAATGCTTAGAAGGGGGAGTGATAGCTGTGTTGCAGTCTTACATTGATGAAGATAAGGATGAGTCTTTTTACACTGTGAGCTGGGCATGCAACGTGGATGGAACACCGTTGCTTGTGGCTGGAGGATTCAATGGTACGATGCGTGTCATTGATTGTGGCAGTGAGAAGATAGACAAGAGTTTTGTTGGCCATGGTGACTCGATAAACGAAATCAGGACTCAGCCATTGAAGTCATCACTTGTAGTGTCAGCAAGCAAAGATGAGTCAGTTCGACTATGGAATGTTCATACTGGAATATGTATTTTGATATTCGCTGGAGCAGGGGGTCATCGAAATGAAGTCTTGAGTGTGGACTTCCATCCATCTGACATTTATCGCATTGCAAGTTGTGGCATGGACAACACAATTAAGATATGGTCGATGAAAGAGTTCTGGACATATGTAGAGAAATCTTTCACATGGACAGATCTTCCATCAAAATTCCCTACAAAATACGTACAATTTCCTGTATTCCTAGCCTCCATTCACACAAACTATGTTGACTGTAATAGGTGGCTTGGAGATTTTATGCTCTCAAAGAGTGTTGACAGTGAAATTGTGCTATGGGAACCAAAAATGAAGGAACAGTCTCCCGGGGAGGGCACCGTTGACATCCTGCAAAAGTACCCTGTTCCAGGGTGTGATATTTGGTTCATCAAGTTTTCCTGTGATTTCCATTATAATGCAGCTGCTATAGGGAATAGAGAAGGAAAGATATTCATTTGGGAACTACAATCGAGCCCCCCAGTCCTTATCGCAAAGTTGTCTCATCCTCAATCAAAATCTCCAATTAGACAAACTGCCATGTCCTTTGATGGAAGCACCATTCTCAGCTGCTGTGAGGACGGCACTATTTGGCGCTGGGATGTCATGGAAAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009409 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009960 GO:0009987 GO:0010154 GO:0010162 GO:0010231 GO:0010431 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090567 GO:0090568 GO:0090696 GO:0097437 GO:0099402 GO:0140110 GO:1901360 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

370

Amino Acids

41.55

Weight (kDa)

5.72

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 41 - 207 1.2e-11 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 85 - 164 3.7e-11 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 95 - 164 5.9e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 98 - 207 2.7e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 100 - 205 1.6e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 100 - 203 1.8e-14 WDR3 first beta-propeller domain
WDR55 PF24796 101 - 207 8.2e-06 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 109 - 211 2.5e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 141 - 207 4.1e-06 WDR3 second beta-propeller domain
WD40 PF00400 172 - 203 1.9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
AccB1I GGYRCC 1 cut(s) 830
AciI CCGC 2 cut(s) 82, 168
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 2 cut(s) 3, 376
AcsI RAATTY 2 cut(s) 668, 1105
AfaI GTAC 4 cut(s) 68, 325, 687, 854
AfiI CCNNNNNNNGG 4 cut(s) 35, 91, 827, 863
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 4 cut(s) 124, 148, 319, 418
AjnI CCWGG 1 cut(s) 862
AluBI AGCT 4 cut(s) 215, 270, 917, 1062
AluI AGCT 4 cut(s) 215, 270, 917, 1062
Alw26I GTCTC 2 cut(s) 822, 999
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 1065
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 2 cut(s) 3, 376
ApeKI GCWGC 3 cut(s) 914, 917, 1062
ApoI RAATTY 2 cut(s) 668, 1105
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AsiGI ACCGGT 1 cut(s) 28
Asp700I GAANNNNTTC 2 cut(s) 617, 641
AspLEI GCGC 1 cut(s) 1089
AsuC2I CCSGG 3 cut(s) 176, 822, 823
AsuHPI GGTGA 3 cut(s) 67, 190, 395
AvaI CYCGRG 1 cut(s) 821
BaeGI GKGCMC 1 cut(s) 833
BalI TGGCCA 2 cut(s) 5, 378
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 2 cut(s) 41, 971
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 3 cut(s) 904, 926, 1049
BccI CCATC 5 cut(s) 281, 555, 559, 670, 1037
BceAI ACGGC 2 cut(s) 98, 1090
BciT130I CCWGG 1 cut(s) 864
BcnI CCSGG 3 cut(s) 176, 822, 823
BcoDI GTCTC 2 cut(s) 822, 999
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 1 cut(s) 1090
BglII AGATCT 1 cut(s) 655
BisI GCNGC 4 cut(s) 169, 915, 918, 1063
BlsI GCNGC 4 cut(s) 170, 916, 919, 1064
Bme1390I CCNGG 4 cut(s) 176, 822, 823, 864
BmeT110I CYCGRG 1 cut(s) 821
BmiI GGNNCC 4 cut(s) 27, 40, 798, 832
BmrFI CCNGG 4 cut(s) 176, 822, 823, 864
BmrI ACTGGG 1 cut(s) 968
BmsI GCATC 1 cut(s) 318
BmuI ACTGGG 1 cut(s) 968
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 2 cut(s) 330, 525
BpuEI CTTGAG 1 cut(s) 553
BpuMI CCSGG 3 cut(s) 176, 822, 823
BsaAI YACGTR 1 cut(s) 685
BsaJI CCNNGG 4 cut(s) 379, 821, 822, 863
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 91, 827, 863
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 484, 974
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 4 cut(s) 379, 821, 822, 863
BseGI GGATG 7 cut(s) 47, 253, 292, 547, 840, 997, 1099
BseLI CCNNNNNNNGG 4 cut(s) 35, 91, 827, 863
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 2 cut(s) 422, 1072
BseNI ACTGG 2 cut(s) 484, 974
BseSI GKGCMC 1 cut(s) 833
BseXI GCAGC 3 cut(s) 904, 926, 1049
BseYI CCCAGC 2 cut(s) 270, 1089
BshFI GGCC 2 cut(s) 5, 378
BshNI GGYRCC 1 cut(s) 830
BshTI ACCGGT 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 3 cut(s) 29, 175, 822
BslI CCNNNNNNNGG 4 cut(s) 35, 91, 827, 863
BsmAI GTCTC 2 cut(s) 822, 999
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 5, 378
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 41, 833, 971
Bsp143I GATC 2 cut(s) 20, 655
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 2 cut(s) 82, 168
BspANI GGCC 2 cut(s) 5, 378
BspCNI CTCAG 2 cut(s) 421, 1071
BspLI GGNNCC 4 cut(s) 27, 40, 798, 832
BspPI GGATC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 567
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 484, 974
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 4 cut(s) 379, 821, 822, 863
BssMI GATC 2 cut(s) 20, 655
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 8 cut(s) 163, 184, 265, 297, 734, 779, 816, 835
Bst6I CTCTTC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 277, 442
BstDEI CTNAG 3 cut(s) 196, 408, 1058
BstDSI CCRYGG 1 cut(s) 379
BstENI CCTNNNNNAGG 2 cut(s) 89, 861
BstF5I GGATG 7 cut(s) 47, 253, 292, 547, 840, 997, 1099
BstH2I RGCGCY 1 cut(s) 1090
BstHHI GCGC 1 cut(s) 1089
BstKTI GATC 2 cut(s) 23, 658
BstMAI GTCTC 2 cut(s) 822, 999
BstMBI GATC 2 cut(s) 20, 655
BstMWI GCNNNNNNNGC 2 cut(s) 11, 276
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 4 cut(s) 174, 820, 821, 862
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 833
BstSNI TACGTA 1 cut(s) 685
BstV1I GCAGC 3 cut(s) 904, 926, 1049
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 655
BstYI RGATCY 1 cut(s) 655
BsuRI GGCC 2 cut(s) 5, 378
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 7 cut(s) 47, 253, 292, 547, 840, 997, 1099
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 3 cut(s) 261, 355, 784
Cac8I GCNNGC 2 cut(s) 277, 442
CaiI CAGNNNCTG 1 cut(s) 1065
CfoI GCGC 1 cut(s) 1089
Cfr10I RCCGGY 1 cut(s) 28
Cfr9I CCCGGG 1 cut(s) 821
Csp6I GTAC 4 cut(s) 67, 324, 686, 853
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 6 cut(s) 276, 380, 583, 648, 1033, 1099
CviQI GTAC 4 cut(s) 67, 324, 686, 853
DdeI CTNAG 3 cut(s) 196, 408, 1058
DpnI GATC 2 cut(s) 22, 657
DpnII GATC 2 cut(s) 20, 655
EaeI YGGCCR 2 cut(s) 3, 376
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 685
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 2 cut(s) 41, 971
Eco88I CYCGRG 1 cut(s) 821
EcoNI CCTNNNNNAGG 2 cut(s) 89, 861
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 41, 971
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 6 cut(s) 279, 383, 586, 651, 1036, 1102
FatI CATG 6 cut(s) 275, 379, 582, 647, 1032, 1098
FauNDI CATATG 1 cut(s) 628
Fnu4HI GCNGC 4 cut(s) 169, 915, 918, 1063
FokI GGATG 7 cut(s) 34, 260, 299, 534, 827, 984, 1106
FriOI GRGCYC 2 cut(s) 41, 971
Fsp4HI GCNGC 4 cut(s) 169, 915, 918, 1063
FspBI CTAG 1 cut(s) 704
GlaI GCGC 1 cut(s) 1088
GluI GCNGC 4 cut(s) 169, 915, 918, 1063
GsaI CCCAGC 2 cut(s) 274, 1093
GsuI CTGGAG 2 cut(s) 330, 525
HaeII RGCGCY 1 cut(s) 1090
HaeIII GGCC 2 cut(s) 5, 378
HapII CCGG 3 cut(s) 29, 175, 822
HhaI GCGC 1 cut(s) 1089
Hin1II CATG 6 cut(s) 279, 383, 586, 651, 1036, 1102
Hin6I GCGC 1 cut(s) 1087
HinP1I GCGC 1 cut(s) 1087
HincII GTYRAC 3 cut(s) 730, 775, 838
HindII GTYRAC 3 cut(s) 730, 775, 838
HinfI GANTC 7 cut(s) 72, 134, 251, 315, 386, 406, 452
HpaII CCGG 3 cut(s) 29, 175, 822
HphI GGTGA 3 cut(s) 67, 190, 395
Hpy166II GTNNAC 4 cut(s) 541, 730, 775, 838
Hpy188I TCNGA 2 cut(s) 25, 556
Hpy188III TCNNGA 3 cut(s) 403, 532, 622
Hpy8I GTNNAC 4 cut(s) 541, 730, 775, 838
Hpy99I CGWCG 2 cut(s) 119, 167
HpyAV CCTTC 3 cut(s) 194, 802, 929
HpyCH4III ACNGT 8 cut(s) 163, 184, 265, 297, 734, 779, 816, 835
HpyCH4IV ACGT 3 cut(s) 150, 282, 684
HpyCH4V TGCA 5 cut(s) 223, 279, 572, 847, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 276
HpyF3I CTNAG 3 cut(s) 196, 408, 1058
HpySE526I ACGT 3 cut(s) 150, 282, 684
Hsp92II CATG 6 cut(s) 279, 383, 586, 651, 1036, 1102
HspAI GCGC 1 cut(s) 1087
Kzo9I GATC 2 cut(s) 20, 655
LmnI GCTCC 2 cut(s) 44, 506
Lsp1109I GCAGC 3 cut(s) 904, 926, 1049
LweI GCATC 1 cut(s) 318
MaeI CTAG 1 cut(s) 704
MaeII ACGT 3 cut(s) 150, 282, 684
MaeIII GTNAC 3 cut(s) 73, 178, 383
MalI GATC 2 cut(s) 22, 657
MboI GATC 2 cut(s) 20, 655
MboII GAAGA 5 cut(s) 70, 145, 251, 367, 650
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 3 cut(s) 41, 833, 971
MlsI TGGCCA 2 cut(s) 5, 378
MluCI AATT 6 cut(s) 594, 668, 689, 783, 1017, 1105
MluNI TGGCCA 2 cut(s) 5, 378
MlyI GAGTC 6 cut(s) 81, 128, 260, 380, 400, 461
MnlI CCTC 9 cut(s) 53, 61, 85, 95, 305, 718, 820, 1011, 1063
Mox20I TGGCCA 2 cut(s) 5, 378
MroXI GAANNNNTTC 2 cut(s) 617, 641
MscI TGGCCA 2 cut(s) 5, 378
MseI TTAA 2 cut(s) 597, 1111
MslI CAYNNNNRTG 2 cut(s) 522, 909
Msp20I TGGCCA 2 cut(s) 5, 378
MspA1I CMGCKG 2 cut(s) 917, 1062
MspI CCGG 3 cut(s) 29, 175, 822
MspR9I CCNGG 4 cut(s) 176, 822, 823, 864
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 864
MwoI GCNNNNNNNGC 2 cut(s) 11, 276
NciI CCSGG 3 cut(s) 176, 822, 823
NcoI CCATGG 1 cut(s) 379
NdeI CATATG 1 cut(s) 628
NdeII GATC 2 cut(s) 20, 655
NlaIII CATG 6 cut(s) 279, 383, 586, 651, 1036, 1102
NlaIV GGNNCC 4 cut(s) 27, 40, 798, 832
NmuCI GTSAC 3 cut(s) 73, 178, 383
NspI RCATGY 1 cut(s) 279
PaeI GCATGC 1 cut(s) 279
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 2 cut(s) 617, 641
PfeI GAWTC 1 cut(s) 315
PinAI ACCGGT 1 cut(s) 28
PkrI GCNGC 4 cut(s) 170, 916, 919, 1064
PleI GAGTC 6 cut(s) 80, 128, 259, 380, 400, 460
PpsI GAGTC 6 cut(s) 80, 128, 259, 380, 400, 460
Ppu21I YACGTR 1 cut(s) 685
PsiI TTATAA 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 862
PspFI CCCAGC 2 cut(s) 270, 1089
PspGI CCWGG 1 cut(s) 862
PspN4I GGNNCC 4 cut(s) 27, 40, 798, 832
PstNI CAGNNNCTG 1 cut(s) 1065
PsuI RGATCY 1 cut(s) 655
PvuII CAGCTG 2 cut(s) 917, 1062
RsaI GTAC 4 cut(s) 68, 325, 687, 854
RsaNI GTAC 4 cut(s) 67, 324, 686, 853
RseI CAYNNNNRTG 2 cut(s) 522, 909
SaqAI TTAA 2 cut(s) 597, 1111
SatI GCNGC 4 cut(s) 169, 915, 918, 1063
Sau3AI GATC 2 cut(s) 20, 655
SchI GAGTC 6 cut(s) 81, 128, 260, 380, 400, 461
ScrFI CCNGG 4 cut(s) 176, 822, 823, 864
SduI GDGCHC 3 cut(s) 41, 833, 971
SetI ASST 8 cut(s) 153, 217, 272, 285, 687, 743, 919, 1064
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 921
SmaI CCCGGG 1 cut(s) 823
SmiMI CAYNNNNRTG 2 cut(s) 522, 909
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
SnaBI TACGTA 1 cut(s) 685
SphI GCATGC 1 cut(s) 279
Sse9I AATT 6 cut(s) 594, 668, 689, 783, 1017, 1105
SsiI CCGC 2 cut(s) 82, 168
SspMI CTAG 1 cut(s) 704
StyD4I CCNGG 4 cut(s) 174, 820, 821, 862
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 8 cut(s) 163, 184, 265, 297, 734, 779, 816, 835
TaiI ACGT 3 cut(s) 153, 285, 687
TaqI TCGA 6 cut(s) 132, 389, 460, 520, 608, 965
TasI AATT 6 cut(s) 594, 668, 689, 783, 1017, 1105
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 2 cut(s) 597, 1111
Tru9I TTAA 2 cut(s) 597, 1111
TscAI CASTG 3 cut(s) 268, 355, 784
TseFI GTSAC 3 cut(s) 73, 178, 383
TseI GCWGC 3 cut(s) 914, 917, 1062
Tsp45I GTSAC 3 cut(s) 73, 178, 383
TspDTI ATGAA 9 cut(s) 118, 204, 252, 464, 540, 626, 821, 871, 937
TspMI CCCGGG 1 cut(s) 821
TspRI CASTG 3 cut(s) 268, 355, 784
XagI CCTNNNNNAGG 2 cut(s) 89, 861
XapI RAATTY 2 cut(s) 668, 1105
XceI RCATGY 1 cut(s) 279
XmaI CCCGGG 1 cut(s) 821
XmnI GAANNNNTTC 2 cut(s) 617, 641
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.