MD11G1227100.v1.1

Polycomb group protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009336
Physical Location & Seq
Forward (+)
33074083 .. 33078069
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1227100.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGCGGCAAAGTTCGCTTTAGGGTCGGAACCGGTGGTGGGTTCGCTCGCACCCTCAAAGAAGAAAGAGTACAGAGTCACCAACAGGCTCCAAGAGGGCAAGCGGCCCCTATACGCCATCGTTTTCAACTTCATCGACTCTCGCTACTTCAACGTCTTCGCCACAGTCGGTGGCAATCGGGTGACTGTATACCAATGCCTAGAAGGGGGTGTGATCGCTGTGTTGCAGTCCTACATTGATGAAGATAAGGATGAGTCTTTTTACACTGTGAGCTGGGCATGCAATAGTGATGGATCCCCATTGCTTGTGGCTGGAGGAATTAATGGTATAATGCGTGTCATCGATTGTGGCAGTGAGAAGATAGACAAGAGTTTTGTTGGCCATGGGGACTCAATAAATGAGATCAGGACGCAACCGTTGAAGTCATCACTTGTGGTGTCAGCAAGCAAAGATGAGTCAGTTCGGCTATGGAATGTTCAGACTGGAATATGCATTTTGATATTTGCTGGAGCAGGGGGTCACCGCAATGAAGTCCTAAGTGTGGACTTTCATCCTTCTGACATATATCGTATTGCGAGTTGTGGCATGGACAACACTGTTAAGATCTGGTCAATGAAAGAGTTCTGGACATATGTAGAGAAATCTTTCACATGGACAGATCTTCCGTCAAAATTTCCTACAAAATATGTGCAATTTCCTGTATTCATAGCTTCCATTCATACAAACTATGTTGACTGTAATAGGTGGCTTGGTGATTTTCTCCTCTCAAAGAGTGTTGACAATGAAATTGTGCTGTGGGAACCGAAAATGAAGGAACAGTCTCCTGGGGAGGGTACTGTTGACATCCTTCAAAAATATCCGGTTCCAGAGTGTGATATTTGGTTCATCAAGTTTTCCTGTGATTTCCACTACAATGCAGCTGCTATAGGGAATAGAGAGGGAAAGATTTTCATTTGGGAACTACAATCCAGCCCTCCTGTTCTTATTGCAAAGTTGTTACATCCTCAATCAAAATCTCCGATTAGGCAAACTGCTACGTCTTTTGATGGAAGCACCATTCTAAGCTGCTGTGAGGACGGAACTATTTGGCGTTGGGATGCTATGGAAAGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009409 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009960 GO:0009987 GO:0010154 GO:0010162 GO:0010231 GO:0010431 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090567 GO:0090568 GO:0090696 GO:0097437 GO:0099402 GO:0140110 GO:1901360 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

372

Amino Acids

41.59

Weight (kDa)

5.63

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 42 - 208 4.1e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 86 - 165 9.8e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 93 - 204 2.1e-14 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 94 - 165 2.7e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 97 - 208 4.1e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 98 - 207 1.8e-16 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 110 - 212 2.3e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 142 - 208 3e-06 WDR3 second beta-propeller domain
WD40 PF00400 173 - 204 2.1e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 189
AciI CCGC 3 cut(s) 5, 103, 523
AclWI GGATC 2 cut(s) 288, 301
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 1 cut(s) 671
AfaI GTAC 2 cut(s) 71, 835
AfiI CCNNNNNNNGG 4 cut(s) 38, 205, 541, 830
AgeI ACCGGT 1 cut(s) 31
AgsI TTSAA 4 cut(s) 127, 151, 421, 851
AjnI CCWGG 1 cut(s) 823
AluBI AGCT 4 cut(s) 273, 710, 920, 1065
AluI AGCT 4 cut(s) 273, 710, 920, 1065
Alw26I GTCTC 1 cut(s) 825
AlwI GGATC 2 cut(s) 288, 301
AoxI GGCC 2 cut(s) 104, 379
ApeKI GCWGC 3 cut(s) 917, 920, 1065
ApoI RAATTY 1 cut(s) 671
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AseI ATTAAT 1 cut(s) 321
AsiGI ACCGGT 1 cut(s) 31
Asp700I GAANNNNTTC 2 cut(s) 620, 644
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 4 cut(s) 70, 193, 512, 764
BalI TGGCCA 1 cut(s) 381
BamHI GGATCC 1 cut(s) 293
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 907, 929, 1052
BccI CCATC 3 cut(s) 125, 284, 1040
BciT130I CCWGG 1 cut(s) 825
BcoDI GTCTC 1 cut(s) 825
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 924
BglII AGATCT 2 cut(s) 603, 658
BisI GCNGC 5 cut(s) 6, 104, 918, 921, 1066
BlsI GCNGC 5 cut(s) 7, 105, 919, 922, 1067
Bme1390I CCNGG 1 cut(s) 825
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 6 cut(s) 30, 89, 107, 295, 801, 864
BmrFI CCNGG 1 cut(s) 825
BmsI GCATC 1 cut(s) 1087
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 2 cut(s) 333, 528
Bsa29I ATCGAT 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 382, 824
BsaWI WCCGGW 2 cut(s) 31, 859
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 205, 541, 830
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 2 cut(s) 299, 532
BseBI CCWGG 1 cut(s) 825
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 382, 824
BseGI GGATG 5 cut(s) 256, 550, 843, 1000, 1102
BseLI CCNNNNNNNGG 4 cut(s) 38, 205, 541, 830
BseMI GCAATG 2 cut(s) 299, 532
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 752
BseXI GCAGC 3 cut(s) 907, 929, 1052
BseYI CCCAGC 1 cut(s) 273
BshFI GGCC 2 cut(s) 106, 381
BshTI ACCGGT 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 342
BsiSI CCGG 2 cut(s) 32, 860
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 4 cut(s) 38, 205, 541, 830
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 2 cut(s) 106, 381
Bsp143I GATC 5 cut(s) 213, 293, 402, 603, 658
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 3 cut(s) 5, 103, 523
BspANI GGCC 2 cut(s) 106, 381
BspDI ATCGAT 1 cut(s) 342
BspLI GGNNCC 6 cut(s) 30, 89, 107, 295, 801, 864
BspPI GGATC 2 cut(s) 288, 301
BsrDI GCAATG 2 cut(s) 299, 532
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 1 cut(s) 487
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 382, 824
BssMI GATC 5 cut(s) 213, 293, 402, 603, 658
BssNAI GTATAC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 382
Bst1107I GTATAC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 8 cut(s) 166, 187, 268, 417, 598, 737, 819, 838
BstC8I GCNNGC 4 cut(s) 48, 101, 280, 445
BstDEI CTNAG 2 cut(s) 536, 1061
BstDSI CCRYGG 1 cut(s) 382
BstEII GGTNACC 1 cut(s) 518
BstF5I GGATG 5 cut(s) 256, 550, 843, 1000, 1102
BstKTI GATC 5 cut(s) 216, 296, 405, 606, 661
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 5 cut(s) 213, 293, 402, 603, 658
BstMWI GCNNNNNNNGC 2 cut(s) 14, 279
BstNI CCWGG 1 cut(s) 825
BstNSI RCATGY 1 cut(s) 282
BstPI GGTNACC 1 cut(s) 518
BstSCI CCNGG 1 cut(s) 823
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 3 cut(s) 907, 929, 1052
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 3 cut(s) 293, 603, 658
BstYI RGATCY 3 cut(s) 293, 603, 658
BstZ17I GTATAC 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 342
BsuRI GGCC 2 cut(s) 106, 381
BsuTUI ATCGAT 1 cut(s) 342
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 5 cut(s) 256, 550, 843, 1000, 1102
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 3 cut(s) 264, 358, 594
Cac8I GCNNGC 4 cut(s) 48, 101, 280, 445
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 105
ClaI ATCGAT 1 cut(s) 342
CseI GACGC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 70, 834
CspAI ACCGGT 1 cut(s) 31
CviAII CATG 4 cut(s) 279, 383, 586, 651
CviQI GTAC 2 cut(s) 70, 834
DdeI CTNAG 2 cut(s) 536, 1061
DpnI GATC 5 cut(s) 215, 295, 404, 605, 660
DpnII GATC 5 cut(s) 213, 293, 402, 603, 658
EaeI YGGCCR 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 382
Eco91I GGTNACC 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 382
EcoT22I ATGCAT 1 cut(s) 494
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 4 cut(s) 282, 386, 589, 654
FaqI GGGAC 1 cut(s) 401
FatI CATG 4 cut(s) 278, 382, 585, 650
FauNDI CATATG 1 cut(s) 631
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 6, 104, 918, 921, 1066
FokI GGATG 5 cut(s) 263, 537, 830, 987, 1109
Fsp4HI GCNGC 5 cut(s) 6, 104, 918, 921, 1066
FspBI CTAG 1 cut(s) 200
GluI GCNGC 5 cut(s) 6, 104, 918, 921, 1066
GsaI CCCAGC 1 cut(s) 277
GsuI CTGGAG 2 cut(s) 333, 528
HaeIII GGCC 2 cut(s) 106, 381
HapII CCGG 2 cut(s) 32, 860
HgaI GACGC 1 cut(s) 418
Hin1II CATG 4 cut(s) 282, 386, 589, 654
HincII GTYRAC 3 cut(s) 733, 778, 841
HindII GTYRAC 3 cut(s) 733, 778, 841
HinfI GANTC 5 cut(s) 75, 137, 254, 389, 455
HpaII CCGG 2 cut(s) 32, 860
HphI GGTGA 4 cut(s) 70, 193, 512, 764
Hpy166II GTNNAC 5 cut(s) 190, 544, 733, 778, 841
Hpy188I TCNGA 4 cut(s) 28, 480, 559, 1020
Hpy188III TCNNGA 3 cut(s) 406, 625, 866
Hpy8I GTNNAC 5 cut(s) 190, 544, 733, 778, 841
HpyAV CCTTC 4 cut(s) 197, 564, 805, 857
HpyCH4III ACNGT 8 cut(s) 166, 187, 268, 417, 598, 737, 819, 838
HpyCH4IV ACGT 2 cut(s) 153, 1037
HpyCH4V TGCA 6 cut(s) 226, 282, 492, 691, 917, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 279
HpyF3I CTNAG 2 cut(s) 536, 1061
HpySE526I ACGT 2 cut(s) 153, 1037
Hsp92II CATG 4 cut(s) 282, 386, 589, 654
Kzo9I GATC 5 cut(s) 213, 293, 402, 603, 658
LmnI GCTCC 2 cut(s) 93, 509
Lsp1109I GCAGC 3 cut(s) 907, 929, 1052
LweI GCATC 1 cut(s) 1087
MaeI CTAG 1 cut(s) 200
MaeII ACGT 2 cut(s) 153, 1037
MaeIII GTNAC 4 cut(s) 76, 181, 518, 996
MalI GATC 5 cut(s) 215, 295, 404, 605, 660
MboI GATC 5 cut(s) 213, 293, 402, 603, 658
MboII GAAGA 5 cut(s) 73, 148, 254, 370, 653
MflI RGATCY 3 cut(s) 293, 603, 658
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 4 cut(s) 318, 671, 692, 786
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 5 cut(s) 84, 131, 263, 383, 464
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 9 cut(s) 64, 88, 308, 773, 823, 931, 984, 1014, 1066
Mox20I TGGCCA 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 494
MroXI GAANNNNTTC 2 cut(s) 620, 644
MscI TGGCCA 1 cut(s) 381
MseI TTAA 3 cut(s) 321, 600, 1114
MslI CAYNNNNRTG 2 cut(s) 525, 912
Msp20I TGGCCA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 2 cut(s) 32, 860
MspR9I CCNGG 1 cut(s) 825
MvaI CCWGG 1 cut(s) 825
MwoI GCNNNNNNNGC 2 cut(s) 14, 279
NcoI CCATGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 631
NdeII GATC 5 cut(s) 213, 293, 402, 603, 658
NlaIII CATG 4 cut(s) 282, 386, 589, 654
NlaIV GGNNCC 6 cut(s) 30, 89, 107, 295, 801, 864
NmuCI GTSAC 3 cut(s) 76, 181, 518
NsiI ATGCAT 1 cut(s) 494
NspI RCATGY 1 cut(s) 282
PaeI GCATGC 1 cut(s) 282
PdmI GAANNNNTTC 2 cut(s) 620, 644
PinAI ACCGGT 1 cut(s) 31
PkrI GCNGC 5 cut(s) 7, 105, 919, 922, 1067
PleI GAGTC 5 cut(s) 83, 131, 262, 383, 463
PpsI GAGTC 5 cut(s) 83, 131, 262, 383, 463
PshBI ATTAAT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 823
PspEI GGTNACC 1 cut(s) 518
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 6 cut(s) 30, 89, 107, 295, 801, 864
PspPI GGNCC 1 cut(s) 105
PsuI RGATCY 3 cut(s) 293, 603, 658
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 2 cut(s) 71, 835
RsaNI GTAC 2 cut(s) 70, 834
RseI CAYNNNNRTG 2 cut(s) 525, 912
SaqAI TTAA 3 cut(s) 321, 600, 1114
SatI GCNGC 5 cut(s) 6, 104, 918, 921, 1066
Sau3AI GATC 5 cut(s) 213, 293, 402, 603, 658
Sau96I GGNCC 1 cut(s) 105
SchI GAGTC 5 cut(s) 84, 131, 263, 383, 464
ScrFI CCNGG 1 cut(s) 825
SetI ASST 7 cut(s) 156, 275, 712, 746, 922, 1040, 1067
SfaNI GCATC 1 cut(s) 1087
SfcI CTRYAG 1 cut(s) 924
SmiMI CAYNNNNRTG 2 cut(s) 525, 912
SphI GCATGC 1 cut(s) 282
Sse9I AATT 4 cut(s) 318, 671, 692, 786
SsiI CCGC 3 cut(s) 5, 103, 523
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 823
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 8 cut(s) 166, 187, 268, 417, 598, 737, 819, 838
TaiI ACGT 2 cut(s) 156, 1040
TaqI TCGA 2 cut(s) 135, 342
TasI AATT 4 cut(s) 318, 671, 692, 786
TatI WGTACW 1 cut(s) 69
TauI GCSGC 2 cut(s) 8, 106
Tru1I TTAA 3 cut(s) 321, 600, 1114
Tru9I TTAA 3 cut(s) 321, 600, 1114
TscAI CASTG 3 cut(s) 271, 358, 601
TseFI GTSAC 3 cut(s) 76, 181, 518
TseI GCWGC 3 cut(s) 917, 920, 1065
Tsp45I GTSAC 3 cut(s) 76, 181, 518
TspGWI ACGGA 2 cut(s) 654, 1092
TspRI CASTG 3 cut(s) 271, 358, 601
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 1 cut(s) 671
XceI RCATGY 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 2 cut(s) 620, 644
XspI CTAG 1 cut(s) 200
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.