Rw5G023520

Polycomb group protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009336
Physical Location & Seq
Reverse (-)
33179525 .. 33183277
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023520.1

Sequence Viewer

Length: 1086 bp
ATGGCCAAGTTCGCTTTAGGATCGGAACCGGTGGTGGGCTCCCTCTCATCCTCCAAGAAGAGAGAGTACAGAGTCACCAACCGCCTCCAAGAGGGCAAGAAACCCATATACGCCGTCGTTTTCAACTTCATCGACTCTCGCTACTTCAACGTCTTCGCCACCGTCGGCGGCAACCGGGTGACTGTGTATGAATGCTTAGAAGGGGGAGTGATAGCTGTGTTGCAGTCTTACATTGATGAAGATCTGGGCATGCAACGTGATGGAACACCGTTGCTTGTGGCTGGAGGATTCAATGGTACGATGCGTGTCATTGATTGTGGCAGTGAGAAGATAGACAAGAGTTTTGTTGGCCATGGTGACTCGATAAACGAAATCAGGACTCAGCCATTGAAGTCATCACTTGTAGTGTCAGCAAGCAAAGATGAGTCAGTTCGACTATGGAATGTTCATACTGGAATATGTATTTTGATATTCGCTGGAGCAGGGGGTCATCGAAATGAAGTCTTGAGTGTGGACTTCCATCCATCTGACATTTATCGCATTGCAAGTTGTGGCATGGACAACACAATTAAGATATGGTCGATGAAAGAGTTCTGGACATATGTAGAGAAATCTTTCACATGGACAGATCTTCCATCAAAATTCCCTACAAAATACGTACAATTTCCTGTATTCCTAGCCTCCATTCACACAAACTATGTTGACTGTAATAGGTGGCTTGGAGATTTTATGCTCTCAAAGAGTGTTGACAGTGAAATTGTGCTATGGGAACCAAAAATGAAGGAGCAGTCTCCCGGGGAGGGCACCGTTGACATCCTGCAAAAGTACCCTGTTCCAGGGTGTGATATTTGGTTCATCAAGTTTTCCTGTGATTTCCATTATAATGCAGCTGCTATAGGGAATAGAGAAGGAAAGATATTCATTTGGGAACTACAATCGAGCCCCCCAGTCCTTATCGCAAAGTTGTCTCATCCTCAATCAAAATCTCCAATTAGACAAACTGCCATGTCCTTTGATGGAAGCACCATTCTCAGCTGCTGTGAGGACGGCACTATTTGGCGCTGGGATGTCATGGAAAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005677 GO:0006139 GO:0006325 GO:0006342 GO:0006349 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009266 GO:0009409 GO:0009628 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009960 GO:0009987 GO:0010154 GO:0010162 GO:0010231 GO:0010431 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0017053 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0021700 GO:0022414 GO:0022611 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031519 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048316 GO:0048367 GO:0048519 GO:0048523 GO:0048580 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051239 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070734 GO:0071514 GO:0071695 GO:0071704 GO:0071840 GO:0080050 GO:0080090 GO:0090567 GO:0090568 GO:0090696 GO:0097437 GO:0099402 GO:0140110 GO:1901360 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:2000014 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

361

Amino Acids

40.5

Weight (kDa)

6.02

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 44 - 198 4.1e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 82 - 155 3.5e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 85 - 198 2e-14 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 87 - 194 1.3e-14 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 91 - 197 1.2e-16 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 91 - 155 9.9e-10 THOC3 beta-propeller domain
WDR55 PF24796 92 - 198 6.4e-06 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 100 - 202 2.1e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 132 - 198 4.5e-06 WDR3 second beta-propeller domain
WD40 PF00400 163 - 194 1.9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AccB1I GGYRCC 1 cut(s) 803
AciI CCGC 2 cut(s) 82, 168
AclWI GGATC 1 cut(s) 28
AcoI YGGCCR 2 cut(s) 3, 349
AcsI RAATTY 2 cut(s) 641, 1078
AfaI GTAC 4 cut(s) 68, 298, 660, 827
AfiI CCNNNNNNNGG 4 cut(s) 35, 91, 800, 836
AgeI ACCGGT 1 cut(s) 28
AgsI TTSAA 4 cut(s) 124, 148, 292, 391
AjnI CCWGG 1 cut(s) 835
AluBI AGCT 3 cut(s) 215, 890, 1035
AluI AGCT 3 cut(s) 215, 890, 1035
Alw26I GTCTC 2 cut(s) 795, 972
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 1038
Ama87I CYCGRG 1 cut(s) 794
AoxI GGCC 2 cut(s) 3, 349
ApeKI GCWGC 3 cut(s) 887, 890, 1035
ApoI RAATTY 2 cut(s) 641, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AsiGI ACCGGT 1 cut(s) 28
Asp700I GAANNNNTTC 2 cut(s) 590, 614
AspLEI GCGC 1 cut(s) 1062
AsuC2I CCSGG 3 cut(s) 176, 795, 796
AsuHPI GGTGA 3 cut(s) 67, 190, 368
AvaI CYCGRG 1 cut(s) 794
BaeGI GKGCMC 1 cut(s) 806
BalI TGGCCA 2 cut(s) 5, 351
BanI GGYRCC 1 cut(s) 803
BanII GRGCYC 2 cut(s) 41, 944
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 145
BbvI GCAGC 3 cut(s) 877, 899, 1022
BccI CCATC 5 cut(s) 254, 528, 532, 643, 1010
BceAI ACGGC 2 cut(s) 98, 1063
BciT130I CCWGG 1 cut(s) 837
BcnI CCSGG 3 cut(s) 176, 795, 796
BcoDI GTCTC 2 cut(s) 795, 972
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 894
BfoI RGCGCY 1 cut(s) 1063
BglII AGATCT 2 cut(s) 241, 628
BisI GCNGC 4 cut(s) 169, 888, 891, 1036
BlsI GCNGC 4 cut(s) 170, 889, 892, 1037
Bme1390I CCNGG 4 cut(s) 176, 795, 796, 837
BmeT110I CYCGRG 1 cut(s) 794
BmiI GGNNCC 4 cut(s) 27, 40, 771, 805
BmrFI CCNGG 4 cut(s) 176, 795, 796, 837
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 1 cut(s) 291
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 145
BpmI CTGGAG 2 cut(s) 303, 498
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 3 cut(s) 176, 795, 796
BsaAI YACGTR 1 cut(s) 658
BsaBI GATNNNNATC 1 cut(s) 240
BsaJI CCNNGG 4 cut(s) 352, 794, 795, 836
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 91, 800, 836
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 457, 947
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 1 cut(s) 837
BseDI CCNNGG 4 cut(s) 352, 794, 795, 836
BseGI GGATG 5 cut(s) 47, 520, 813, 970, 1072
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 4 cut(s) 35, 91, 800, 836
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 2 cut(s) 395, 1045
BseNI ACTGG 2 cut(s) 457, 947
BseSI GKGCMC 1 cut(s) 806
BseXI GCAGC 3 cut(s) 877, 899, 1022
BseYI CCCAGC 1 cut(s) 1062
BshFI GGCC 2 cut(s) 5, 351
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 1 cut(s) 28
BsiHKCI CYCGRG 1 cut(s) 794
BsiSI CCGG 3 cut(s) 29, 175, 795
BslI CCNNNNNNNGG 4 cut(s) 35, 91, 800, 836
BsmAI GTCTC 2 cut(s) 795, 972
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 2 cut(s) 5, 351
BsoBI CYCGRG 1 cut(s) 794
Bsp1286I GDGCHC 3 cut(s) 41, 806, 944
Bsp143I GATC 3 cut(s) 20, 241, 628
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 2 cut(s) 82, 168
BspANI GGCC 2 cut(s) 5, 351
BspCNI CTCAG 2 cut(s) 394, 1044
BspLI GGNNCC 4 cut(s) 27, 40, 771, 805
BspPI GGATC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 457, 947
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 4 cut(s) 352, 794, 795, 836
BssMI GATC 3 cut(s) 20, 241, 628
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 837
Bst4CI ACNGT 6 cut(s) 163, 184, 270, 707, 752, 808
Bst6I CTCTTC 1 cut(s) 53
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 251, 415
BstDEI CTNAG 3 cut(s) 196, 381, 1031
BstDSI CCRYGG 1 cut(s) 352
BstENI CCTNNNNNAGG 2 cut(s) 89, 834
BstF5I GGATG 5 cut(s) 47, 520, 813, 970, 1072
BstH2I RGCGCY 1 cut(s) 1063
BstHHI GCGC 1 cut(s) 1062
BstKTI GATC 3 cut(s) 23, 244, 631
BstMAI GTCTC 2 cut(s) 795, 972
BstMBI GATC 3 cut(s) 20, 241, 628
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 837
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 4 cut(s) 174, 793, 794, 835
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 1 cut(s) 806
BstSNI TACGTA 1 cut(s) 658
BstV1I GCAGC 3 cut(s) 877, 899, 1022
BstV2I GAAGAC 1 cut(s) 145
BstX2I RGATCY 2 cut(s) 241, 628
BstYI RGATCY 2 cut(s) 241, 628
BsuRI GGCC 2 cut(s) 5, 351
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 5 cut(s) 47, 520, 813, 970, 1072
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 2 cut(s) 328, 757
Cac8I GCNNGC 2 cut(s) 251, 415
CaiI CAGNNNCTG 1 cut(s) 1038
CfoI GCGC 1 cut(s) 1062
Cfr10I RCCGGY 1 cut(s) 28
Cfr9I CCCGGG 1 cut(s) 794
Csp6I GTAC 4 cut(s) 67, 297, 659, 826
CspAI ACCGGT 1 cut(s) 28
CviAII CATG 6 cut(s) 250, 353, 556, 621, 1006, 1072
CviQI GTAC 4 cut(s) 67, 297, 659, 826
DdeI CTNAG 3 cut(s) 196, 381, 1031
DpnI GATC 3 cut(s) 22, 243, 630
DpnII GATC 3 cut(s) 20, 241, 628
EaeI YGGCCR 2 cut(s) 3, 349
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 2 cut(s) 41, 944
Eco88I CYCGRG 1 cut(s) 794
EcoNI CCTNNNNNAGG 2 cut(s) 89, 834
EcoRII CCWGG 1 cut(s) 835
EcoT14I CCWWGG 1 cut(s) 352
EcoT38I GRGCYC 2 cut(s) 41, 944
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 6 cut(s) 253, 356, 559, 624, 1009, 1075
FatI CATG 6 cut(s) 249, 352, 555, 620, 1005, 1071
FauNDI CATATG 1 cut(s) 601
Fnu4HI GCNGC 4 cut(s) 169, 888, 891, 1036
FokI GGATG 5 cut(s) 34, 507, 800, 957, 1079
FriOI GRGCYC 2 cut(s) 41, 944
Fsp4HI GCNGC 4 cut(s) 169, 888, 891, 1036
FspBI CTAG 1 cut(s) 677
GlaI GCGC 1 cut(s) 1061
GluI GCNGC 4 cut(s) 169, 888, 891, 1036
GsaI CCCAGC 1 cut(s) 1066
GsuI CTGGAG 2 cut(s) 303, 498
HaeII RGCGCY 1 cut(s) 1063
HaeIII GGCC 2 cut(s) 5, 351
HapII CCGG 3 cut(s) 29, 175, 795
HhaI GCGC 1 cut(s) 1062
Hin1II CATG 6 cut(s) 253, 356, 559, 624, 1009, 1075
Hin6I GCGC 1 cut(s) 1060
HinP1I GCGC 1 cut(s) 1060
HincII GTYRAC 3 cut(s) 703, 748, 811
HindII GTYRAC 3 cut(s) 703, 748, 811
HinfI GANTC 6 cut(s) 72, 134, 288, 359, 379, 425
HpaII CCGG 3 cut(s) 29, 175, 795
HphI GGTGA 3 cut(s) 67, 190, 368
Hpy166II GTNNAC 4 cut(s) 514, 703, 748, 811
Hpy188I TCNGA 2 cut(s) 25, 529
Hpy188III TCNNGA 3 cut(s) 376, 505, 595
Hpy8I GTNNAC 4 cut(s) 514, 703, 748, 811
Hpy99I CGWCG 2 cut(s) 119, 167
HpyAV CCTTC 3 cut(s) 194, 775, 902
HpyCH4III ACNGT 6 cut(s) 163, 184, 270, 707, 752, 808
HpyCH4IV ACGT 3 cut(s) 150, 256, 657
HpyCH4V TGCA 5 cut(s) 223, 253, 545, 820, 887
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 196, 381, 1031
HpySE526I ACGT 3 cut(s) 150, 256, 657
Hsp92II CATG 6 cut(s) 253, 356, 559, 624, 1009, 1075
HspAI GCGC 1 cut(s) 1060
Kzo9I GATC 3 cut(s) 20, 241, 628
LmnI GCTCC 3 cut(s) 44, 479, 784
Lsp1109I GCAGC 3 cut(s) 877, 899, 1022
LweI GCATC 1 cut(s) 291
MaeI CTAG 1 cut(s) 677
MaeII ACGT 3 cut(s) 150, 256, 657
MaeIII GTNAC 3 cut(s) 73, 178, 356
MalI GATC 3 cut(s) 22, 243, 630
MboI GATC 3 cut(s) 20, 241, 628
MboII GAAGA 5 cut(s) 70, 145, 251, 340, 623
MflI RGATCY 2 cut(s) 241, 628
MhlI GDGCHC 3 cut(s) 41, 806, 944
MlsI TGGCCA 2 cut(s) 5, 351
MluCI AATT 6 cut(s) 567, 641, 662, 756, 990, 1078
MluNI TGGCCA 2 cut(s) 5, 351
MlyI GAGTC 5 cut(s) 81, 128, 353, 373, 434
MnlI CCTC 9 cut(s) 53, 61, 85, 95, 278, 691, 793, 984, 1036
Mox20I TGGCCA 2 cut(s) 5, 351
MroXI GAANNNNTTC 2 cut(s) 590, 614
MscI TGGCCA 2 cut(s) 5, 351
MseI TTAA 2 cut(s) 570, 1084
MslI CAYNNNNRTG 2 cut(s) 495, 882
Msp20I TGGCCA 2 cut(s) 5, 351
MspA1I CMGCKG 2 cut(s) 890, 1035
MspI CCGG 3 cut(s) 29, 175, 795
MspR9I CCNGG 4 cut(s) 176, 795, 796, 837
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 837
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 3 cut(s) 176, 795, 796
NcoI CCATGG 1 cut(s) 352
NdeI CATATG 1 cut(s) 601
NdeII GATC 3 cut(s) 20, 241, 628
NlaIII CATG 6 cut(s) 253, 356, 559, 624, 1009, 1075
NlaIV GGNNCC 4 cut(s) 27, 40, 771, 805
NmuCI GTSAC 3 cut(s) 73, 178, 356
NspI RCATGY 1 cut(s) 253
PaeI GCATGC 1 cut(s) 253
PctI GAATGC 1 cut(s) 197
PdmI GAANNNNTTC 2 cut(s) 590, 614
PfeI GAWTC 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 28
PkrI GCNGC 4 cut(s) 170, 889, 892, 1037
PleI GAGTC 5 cut(s) 80, 128, 353, 373, 433
PpsI GAGTC 5 cut(s) 80, 128, 353, 373, 433
Ppu21I YACGTR 1 cut(s) 658
PsiI TTATAA 1 cut(s) 882
Psp6I CCWGG 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 1062
PspGI CCWGG 1 cut(s) 835
PspN4I GGNNCC 4 cut(s) 27, 40, 771, 805
PstNI CAGNNNCTG 1 cut(s) 1038
PsuI RGATCY 2 cut(s) 241, 628
PvuII CAGCTG 2 cut(s) 890, 1035
RsaI GTAC 4 cut(s) 68, 298, 660, 827
RsaNI GTAC 4 cut(s) 67, 297, 659, 826
RseI CAYNNNNRTG 2 cut(s) 495, 882
SaqAI TTAA 2 cut(s) 570, 1084
SatI GCNGC 4 cut(s) 169, 888, 891, 1036
Sau3AI GATC 3 cut(s) 20, 241, 628
SchI GAGTC 5 cut(s) 81, 128, 353, 373, 434
ScrFI CCNGG 4 cut(s) 176, 795, 796, 837
SduI GDGCHC 3 cut(s) 41, 806, 944
SetI ASST 7 cut(s) 153, 217, 259, 660, 716, 892, 1037
SfaNI GCATC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 894
SmaI CCCGGG 1 cut(s) 796
SmiMI CAYNNNNRTG 2 cut(s) 495, 882
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
SnaBI TACGTA 1 cut(s) 658
SphI GCATGC 1 cut(s) 253
Sse9I AATT 6 cut(s) 567, 641, 662, 756, 990, 1078
SsiI CCGC 2 cut(s) 82, 168
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 174, 793, 794, 835
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 6 cut(s) 163, 184, 270, 707, 752, 808
TaiI ACGT 3 cut(s) 153, 259, 660
TaqI TCGA 6 cut(s) 132, 362, 433, 493, 581, 938
TasI AATT 6 cut(s) 567, 641, 662, 756, 990, 1078
TatI WGTACW 1 cut(s) 66
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 2 cut(s) 570, 1084
Tru9I TTAA 2 cut(s) 570, 1084
TscAI CASTG 2 cut(s) 328, 757
TseFI GTSAC 3 cut(s) 73, 178, 356
TseI GCWGC 3 cut(s) 887, 890, 1035
Tsp45I GTSAC 3 cut(s) 73, 178, 356
TspDTI ATGAA 9 cut(s) 118, 204, 252, 437, 513, 599, 794, 844, 910
TspMI CCCGGG 1 cut(s) 794
TspRI CASTG 2 cut(s) 328, 757
XagI CCTNNNNNAGG 2 cut(s) 89, 834
XapI RAATTY 2 cut(s) 641, 1078
XceI RCATGY 1 cut(s) 253
XmaI CCCGGG 1 cut(s) 794
XmnI GAANNNNTTC 2 cut(s) 590, 614
XspI CTAG 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.