AT3G21300
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007866
Physical Location & Seq
Reverse (-)
7492739 .. 7496371
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21300.1

Sequence Viewer

Length: 1665 bp
ATGTTGGCCGCGACGCCTGCTCATGGACTCAGGTACGTGGTGGTGCCCACACGCTCATGGCTTCATAGGTTTCGATGCTGCTCGTCTTCTCTCGCTCCTCTCTCCCCTTCTGAATTCTCGGGCGGTAATAAAGCCCAGAAGAAGCTCAACGACGAGAACAACACCAACGTAAGCAATGAGAAAACGGCGCCGTATTATCCGAAACGAGGAGAAACAGTGGAGCTTGTGTGCGAGAGTTTAGGATTCAAAGGCAAAGGAATCTGTAAAGTTGATGGAACTGGCTACGTCGTTATGTGTGACCGAGCTCTTCCCGGCGAACGTTTTCTTGGCCGTGTCACTCGCCGCAAAGGCAGCTATGCTGAAGTGACAAAAATGAAGACAATATCTCCACATAAGGACTTAGTAGAAGCTCCATGTGAGTATGCATCTTACTGTGGTGGTTGCAAAACTCAGAATCTCTCTTATGAAGCTCAGCTTAGAGCTAAAGAAGAGCAAGTTCATGAGCTTATCAGACATGTTGGAAGGTTTTCTGATAACAATCCTGGCCTTGAGATTGTCTTGAAGCCTATTGTTGCTTGCGATATTCAATTCCATTATCGGAATAAGATGGAGTTTTCATTTGGTCCACAAAGATGGCTTCCGATTGAAATGTTAAACGAGAGACAAGATGGTCCTAAGAACTTTGCATTGGGGCTCCATGCACCTGGATTTTTTGATAAGGTTTTAAATGTTGATAAGTGCTTATTACAGAGCGAGCCAGGAAACTTGGTTCTTGCTGCTGTCCAAGATTGCTGGAGAGATCCTGAATTAAGTCTTTCACCATATGATTGTCGTTCACATGTTGGGTTTCTTAAACATTTGATGCTGAGAACTGGAAGGAATGTGGAGACTGGTTCACTAGAACTTATGGTCAATTTTGTGACATCGTCTTATAAGCCAGAGCTGTTGAAACCCCTGGTTGATAGGATTTCATCAATTCCTCAAGTGGTAAGCATTATGAATAATGTAAATTCTTCGGTAGGAAACACATCCGTTGGCGAAAAGGAATATACTCTTTATGGGAAGGATACAATCACAGAGGTCTTAAGAGGGCTTACATTTCAAATTTCAGCCAACTCTTTCTTTCAGACTAACACTCATCAGGCTGAAGTTTTATATAAGCTTATTGAGGAATCTGCTGGACTTAAAGGAGATGGCTCAGAAGTCGTGCTCGATCTTTTCTGTGGAACTGGTACCATAGGCCTTACACTTGCCAGAAGGGCGAAACACGTGTATGGTTATGAAGTAGTACCACAAGCAATAACAGATGCACACAAGAATGCCCAAATAAACGGCATAGAGAATGCAACATTCATCCAAGGGGATCTAAACAAAATAGGAGAAGATTTTGGGAACAATTTCCCTAAGCCTGATATCGTTATTTCTGATCCCAATCGACCTGGTATGCACATGAAGTTGATCAAGTTTCTGCTAAAGCTTAAATCTCCACGGATCATTTATGTTTCCTGTAATCCTGCAACCTGCGCCAGGGATCTCGATTACCTCTGTCACGGCGTGGAGGAGAAGAATCTAAAAGGTTGCTACAAACTGATGAGTGTTCAGCCAGTGGATATGTTCCCTCACACTCCTCACATCGAATGTGTCTGCTTGTTAGAGCTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

554

Amino Acids

61.73

Weight (kDa)

7.99

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 355 - 552 1.2e-16 tRNA (Uracil-5-)-methyltransferase
TRM5-TYW2_MTfase PF02475 399 - 459 2.4e-06 TRM5/TYW2 methyltransferase domain
MTS PF05175 399 - 461 4.9e-06 Methyltransferase small domain
Methyltransf_31 PF13847 401 - 505 8.7e-14 Methyltransferase domain
Methyltransf_15 PF09445 401 - 478 2.5e-07 RNA cap guanine-N2 methyltransferase
Methyltransf_25 PF13649 403 - 460 4.1e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
AasI GACNNNNNNGTC 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 1529
Acc65I GGTACC 1 cut(s) 1232
AccB1I GGYRCC 3 cut(s) 43, 187, 1232
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 9, 123, 343
AclI AACGTT 1 cut(s) 319
AclWI GGATC 5 cut(s) 794, 1371, 1421, 1499, 1539
AcoI YGGCCR 2 cut(s) 6, 328
AcsI RAATTY 3 cut(s) 113, 1009, 1104
AcuI CTGAAG 2 cut(s) 381, 1167
AcvI CACGTG 1 cut(s) 1270
AcyI GRCGYC 2 cut(s) 14, 188
AdeI CACNNNGTG 1 cut(s) 1555
AfaI GTAC 3 cut(s) 35, 1234, 1290
AfiI CCNNNNNNNGG 3 cut(s) 23, 206, 1527
AflII CTTAAG 1 cut(s) 1084
AflIII ACRYGT 4 cut(s) 514, 838, 1267, 1269
AgsI TTSAA 6 cut(s) 247, 562, 587, 647, 949, 1103
AjnI CCWGG 6 cut(s) 541, 703, 757, 954, 1438, 1526
AjuI GAANNNNNNNTTGG 4 cut(s) 309, 341, 671, 703
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
Alw21I GWGCWC 2 cut(s) 307, 1212
Alw26I GTCTC 2 cut(s) 655, 881
AlwI GGATC 5 cut(s) 794, 1371, 1421, 1499, 1539
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 4 cut(s) 6, 328, 544, 1240
ApeKI GCWGC 3 cut(s) 78, 351, 776
ApoI RAATTY 3 cut(s) 113, 1009, 1104
Asp700I GAANNNNTTC 3 cut(s) 321, 526, 1397
Asp718I GGTACC 1 cut(s) 1232
AspLEI GCGC 2 cut(s) 190, 1526
AspS9I GGNCC 2 cut(s) 623, 671
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 810
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 2 cut(s) 623, 671
BaeGI GKGCMC 1 cut(s) 48
BanI GGYRCC 3 cut(s) 43, 187, 1232
BanII GRGCYC 2 cut(s) 307, 696
BbrPI CACGTG 1 cut(s) 1270
BbsI GAAGAC 2 cut(s) 78, 383
Bbv12I GWGCWC 2 cut(s) 307, 1212
BbvI GCAGC 3 cut(s) 65, 363, 763
BccI CCATC 5 cut(s) 266, 601, 627, 662, 1187
BceAI ACGGC 5 cut(s) 175, 201, 315, 1348, 1567
BciT130I CCWGG 6 cut(s) 543, 705, 759, 956, 1440, 1528
BciVI GTATCC 1 cut(s) 1060
BclI TGATCA 1 cut(s) 1458
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 655, 881
BfaI CTAG 1 cut(s) 899
BfoI RGCGCY 1 cut(s) 191
BfrI CTTAAG 1 cut(s) 1084
BfuAI ACCTGC 1 cut(s) 1529
BfuI GTATCC 1 cut(s) 1060
BglI GCCNNNNNGGC 2 cut(s) 348, 1259
BisI GCNGC 5 cut(s) 9, 79, 343, 352, 777
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 5 cut(s) 10, 80, 344, 353, 778
Bme1390I CCNGG 7 cut(s) 312, 543, 705, 759, 956, 1440, 1528
Bme18I GGWCC 2 cut(s) 623, 671
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 623, 671
BmiI GGNNCC 4 cut(s) 45, 189, 695, 1234
BmrFI CCNGG 7 cut(s) 312, 543, 705, 759, 956, 1440, 1528
BmsI GCATC 4 cut(s) 65, 434, 852, 1297
BpiI GAAGAC 2 cut(s) 78, 383
BpmI CTGGAG 1 cut(s) 814
Bpu10I CCTNAGC 1 cut(s) 1404
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 2 cut(s) 569, 966
BpuMI CCSGG 1 cut(s) 312
BsaAI YACGTR 2 cut(s) 37, 1270
BsaBI GATNNNNATC 2 cut(s) 537, 1431
BsaHI GRCGYC 2 cut(s) 14, 188
BsaJI CCNNGG 4 cut(s) 954, 1357, 1487, 1527
BsaXI ACNNNNNCTCC 6 cut(s) 201, 212, 231, 242, 370, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 206, 1527
Bse1I ACTGG 5 cut(s) 283, 877, 895, 1234, 1604
Bse3DI GCAATG 1 cut(s) 181
Bse8I GATNNNNATC 2 cut(s) 537, 1431
BseBI CCWGG 6 cut(s) 543, 705, 759, 956, 1440, 1528
BseDI CCNNGG 4 cut(s) 954, 1357, 1487, 1527
BseGI GGATG 2 cut(s) 1028, 1353
BseJI GATNNNNATC 2 cut(s) 537, 1431
BseLI CCNNNNNNNGG 3 cut(s) 23, 206, 1527
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 5 cut(s) 43, 464, 485, 857, 1212
BseNI ACTGG 5 cut(s) 283, 877, 895, 1234, 1604
BseRI GAGGAG 4 cut(s) 87, 222, 1574, 1617
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 3 cut(s) 65, 363, 763
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 4 cut(s) 8, 330, 546, 1242
BshNI GGYRCC 3 cut(s) 43, 187, 1232
BsiHKAI GWGCWC 2 cut(s) 307, 1212
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 312
BslI CCNNNNNNNGG 3 cut(s) 23, 206, 1527
BsmAI GTCTC 2 cut(s) 655, 881
BsmI GAATGC 2 cut(s) 1324, 1348
BsnI GGCC 4 cut(s) 8, 330, 546, 1242
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 4 cut(s) 48, 307, 696, 1212
Bsp143I GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
Bsp1720I GCTNAGC 1 cut(s) 471
BspACI CCGC 3 cut(s) 9, 123, 343
BspANI GGCC 4 cut(s) 8, 330, 546, 1242
BspCNI CTCAG 5 cut(s) 42, 463, 484, 858, 1211
BspFNI CGCG 1 cut(s) 11
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 45, 189, 695, 1234
BspMI ACCTGC 1 cut(s) 1529
BspPI GGATC 5 cut(s) 794, 1371, 1421, 1499, 1539
BspQI GCTCTTC 2 cut(s) 312, 483
BspT107I GGYRCC 3 cut(s) 43, 187, 1232
BspTI CTTAAG 1 cut(s) 1084
BsrDI GCAATG 1 cut(s) 181
BsrI ACTGG 5 cut(s) 283, 877, 895, 1234, 1604
BssECI CCNNGG 4 cut(s) 954, 1357, 1487, 1527
BssMI GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
BssNI GRCGYC 2 cut(s) 14, 188
BssT1I CCWWGG 1 cut(s) 1357
Bst2UI CCWGG 6 cut(s) 543, 705, 759, 956, 1440, 1528
Bst4CI ACNGT 2 cut(s) 217, 434
Bst6I CTCTTC 2 cut(s) 312, 483
BstACI GRCGYC 2 cut(s) 14, 188
BstAFI CTTAAG 1 cut(s) 1084
BstBAI YACGTR 2 cut(s) 37, 1270
BstC8I GCNNGC 4 cut(s) 18, 577, 755, 1659
BstDEI CTNAG 9 cut(s) 29, 400, 450, 471, 476, 675, 866, 1198, 1404
BstDSI CCRYGG 1 cut(s) 1487
BstENI CCTNNNNNAGG 1 cut(s) 1525
BstF5I GGATG 2 cut(s) 1028, 1353
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 2 cut(s) 190, 1526
BstKTI GATC 7 cut(s) 802, 1216, 1366, 1429, 1461, 1494, 1534
BstMAI GTCTC 2 cut(s) 655, 881
BstMBI GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
BstMWI GCNNNNNNNGC 5 cut(s) 17, 348, 351, 1259, 1523
BstNI CCWGG 6 cut(s) 543, 705, 759, 956, 1440, 1528
BstNSI RCATGY 2 cut(s) 518, 842
BstSCI CCNGG 7 cut(s) 310, 541, 703, 757, 954, 1438, 1526
BstSLI GKGCMC 1 cut(s) 48
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 65, 363, 763
BstV2I GAAGAC 2 cut(s) 78, 383
BstX2I RGATCY 3 cut(s) 799, 1363, 1531
BstXI CCANNNNNNTGG 2 cut(s) 633, 704
BstYI RGATCY 3 cut(s) 799, 1363, 1531
BsuI GTATCC 1 cut(s) 1060
BsuRI GGCC 4 cut(s) 8, 330, 546, 1242
BtgI CCRYGG 1 cut(s) 1487
BtsCI GGATG 2 cut(s) 1028, 1353
BtsIMutI CAGTG 2 cut(s) 222, 1611
BveI ACCTGC 1 cut(s) 1529
Cac8I GCNNGC 4 cut(s) 18, 577, 755, 1659
CciI TCATGA 1 cut(s) 499
CfoI GCGC 2 cut(s) 190, 1526
Cfr13I GGNCC 2 cut(s) 623, 671
CseI GACGC 1 cut(s) 22
CsiI ACCWGGT 1 cut(s) 1438
Csp6I GTAC 3 cut(s) 34, 1233, 1289
CviAII CATG 8 cut(s) 23, 57, 414, 500, 515, 698, 839, 1450
CviQI GTAC 3 cut(s) 34, 1233, 1289
DdeI CTNAG 9 cut(s) 29, 400, 450, 471, 476, 675, 866, 1198, 1404
DinI GGCGCC 1 cut(s) 189
DpnI GATC 7 cut(s) 801, 1215, 1365, 1428, 1460, 1493, 1533
DpnII GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
DraI TTTAAA 1 cut(s) 726
DraIII CACNNNGTG 1 cut(s) 1555
DrdI GACNNNNNNGTC 1 cut(s) 669
DseDI GACNNNNNNGTC 1 cut(s) 669
EaeI YGGCCR 2 cut(s) 6, 328
Eam1104I CTCTTC 2 cut(s) 312, 483
EarI CTCTTC 2 cut(s) 312, 483
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 1357
Eco147I AGGCCT 1 cut(s) 1242
Eco24I GRGCYC 2 cut(s) 307, 696
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 2 cut(s) 623, 671
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 381, 1167
Eco72I CACGTG 1 cut(s) 1270
Eco88I CYCGRG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 1525
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 6 cut(s) 541, 703, 757, 954, 1438, 1526
EcoRV GATATC 1 cut(s) 1414
EcoT14I CCWWGG 1 cut(s) 1357
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 307, 696
EgeI GGCGCC 1 cut(s) 189
EheI GGCGCC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 1357
FaeI CATG 8 cut(s) 26, 60, 417, 503, 518, 701, 842, 1453
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FatI CATG 8 cut(s) 22, 56, 413, 499, 514, 697, 838, 1449
FauNDI CATATG 1 cut(s) 823
FbaI TGATCA 1 cut(s) 1458
Fnu4HI GCNGC 5 cut(s) 9, 79, 343, 352, 777
FokI GGATG 2 cut(s) 1015, 1340
FriOI GRGCYC 2 cut(s) 307, 696
Fsp4HI GCNGC 5 cut(s) 9, 79, 343, 352, 777
FspBI CTAG 1 cut(s) 899
GlaI GCGC 2 cut(s) 189, 1525
GluI GCNGC 5 cut(s) 9, 79, 343, 352, 777
GsuI CTGGAG 1 cut(s) 814
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 4 cut(s) 8, 330, 546, 1242
HapII CCGG 1 cut(s) 312
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 190, 1526
Hin1I GRCGYC 2 cut(s) 14, 188
Hin1II CATG 8 cut(s) 26, 60, 417, 503, 518, 701, 842, 1453
Hin6I GCGC 2 cut(s) 188, 1524
HinP1I GCGC 2 cut(s) 188, 1524
HindIII AAGCTT 2 cut(s) 1160, 1475
HinfI GANTC 6 cut(s) 27, 243, 258, 454, 1172, 1567
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 810
Hpy166II GTNNAC 3 cut(s) 626, 836, 896
Hpy188III TCNNGA 4 cut(s) 500, 559, 803, 1535
Hpy8I GTNNAC 3 cut(s) 626, 836, 896
Hpy99I CGWCG 3 cut(s) 16, 155, 290
HpyAV CCTTC 5 cut(s) 117, 516, 870, 1057, 1251
HpyCH4III ACNGT 2 cut(s) 217, 434
HpyCH4IV ACGT 5 cut(s) 36, 168, 285, 319, 1269
HpyCH4V TGCA 8 cut(s) 425, 444, 686, 701, 1310, 1346, 1447, 1517
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 348, 351, 1259, 1523
HpyF3I CTNAG 9 cut(s) 29, 400, 450, 471, 476, 675, 866, 1198, 1404
HpySE526I ACGT 5 cut(s) 36, 168, 285, 319, 1269
Hsp92I GRCGYC 2 cut(s) 14, 188
Hsp92II CATG 8 cut(s) 26, 60, 417, 503, 518, 701, 842, 1453
HspAI GCGC 2 cut(s) 188, 1524
KasI GGCGCC 1 cut(s) 187
KpnI GGTACC 1 cut(s) 1236
Ksp22I TGATCA 1 cut(s) 1458
Kzo9I GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
LguI GCTCTTC 2 cut(s) 312, 483
LmnI GCTCC 4 cut(s) 100, 220, 415, 699
Lsp1109I GCAGC 3 cut(s) 65, 363, 763
LweI GCATC 4 cut(s) 65, 434, 852, 1297
MabI ACCWGGT 1 cut(s) 1438
MaeI CTAG 1 cut(s) 899
MaeII ACGT 5 cut(s) 36, 168, 285, 319, 1269
MaeIII GTNAC 5 cut(s) 296, 334, 364, 919, 1547
MalI GATC 7 cut(s) 801, 1215, 1365, 1428, 1460, 1493, 1533
MboI GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
MboII GAAGA 8 cut(s) 78, 151, 299, 388, 500, 1005, 1394, 1576
MflI RGATCY 3 cut(s) 799, 1363, 1531
MhlI GDGCHC 4 cut(s) 48, 307, 696, 1212
MluCI AATT 8 cut(s) 113, 587, 806, 913, 975, 1009, 1104, 1396
Mly113I GGCGCC 1 cut(s) 188
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 499
Mph1103I ATGCAT 1 cut(s) 427
MroXI GAANNNNTTC 3 cut(s) 321, 526, 1397
MseI TTAA 7 cut(s) 653, 725, 809, 852, 1085, 1185, 1479
MslI CAYNNNNRTG 4 cut(s) 55, 631, 1272, 1317
MspCI CTTAAG 1 cut(s) 1084
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 7 cut(s) 312, 543, 705, 759, 956, 1440, 1528
Mva1269I GAATGC 2 cut(s) 1324, 1348
MvaI CCWGG 6 cut(s) 543, 705, 759, 956, 1440, 1528
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 5 cut(s) 17, 348, 351, 1259, 1523
NarI GGCGCC 1 cut(s) 188
NciI CCSGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 823
NdeII GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
NlaIII CATG 8 cut(s) 26, 60, 417, 503, 518, 701, 842, 1453
NlaIV GGNNCC 4 cut(s) 45, 189, 695, 1234
NmuCI GTSAC 5 cut(s) 296, 334, 364, 919, 1547
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 2 cut(s) 518, 842
PagI TCATGA 1 cut(s) 499
PceI AGGCCT 1 cut(s) 1242
PciI ACATGT 2 cut(s) 514, 838
PciSI GCTCTTC 2 cut(s) 312, 483
PctI GAATGC 2 cut(s) 1324, 1348
PdmI GAANNNNTTC 3 cut(s) 321, 526, 1397
PfeI GAWTC 5 cut(s) 243, 258, 454, 1172, 1567
PflFI GACNNNGTC 1 cut(s) 925
PkrI GCNGC 5 cut(s) 10, 80, 344, 353, 778
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 191
PmaCI CACGTG 1 cut(s) 1270
PmlI CACGTG 1 cut(s) 1270
PpsI GAGTC 1 cut(s) 21
Ppu21I YACGTR 2 cut(s) 37, 1270
PscI ACATGT 2 cut(s) 514, 838
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 307
Psp1406I AACGTT 1 cut(s) 319
Psp6I CCWGG 6 cut(s) 541, 703, 757, 954, 1438, 1526
PspCI CACGTG 1 cut(s) 1270
PspGI CCWGG 6 cut(s) 541, 703, 757, 954, 1438, 1526
PspN4I GGNNCC 4 cut(s) 45, 189, 695, 1234
PspPI GGNCC 2 cut(s) 623, 671
PsuI RGATCY 3 cut(s) 799, 1363, 1531
PsyI GACNNNGTC 1 cut(s) 925
RsaI GTAC 3 cut(s) 35, 1234, 1290
RsaNI GTAC 3 cut(s) 34, 1233, 1289
RseI CAYNNNNRTG 4 cut(s) 55, 631, 1272, 1317
SacI GAGCTC 1 cut(s) 307
SapI GCTCTTC 2 cut(s) 312, 483
SaqAI TTAA 7 cut(s) 653, 725, 809, 852, 1085, 1185, 1479
SatI GCNGC 5 cut(s) 9, 79, 343, 352, 777
Sau3AI GATC 7 cut(s) 799, 1213, 1363, 1426, 1458, 1491, 1531
Sau96I GGNCC 2 cut(s) 623, 671
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 7 cut(s) 312, 543, 705, 759, 956, 1440, 1528
SduI GDGCHC 4 cut(s) 48, 307, 696, 1212
SexAI ACCWGGT 1 cut(s) 1438
SfaNI GCATC 4 cut(s) 65, 434, 852, 1297
SfoI GGCGCC 1 cut(s) 189
SinI GGWCC 2 cut(s) 623, 671
SmiMI CAYNNNNRTG 4 cut(s) 55, 631, 1272, 1317
SmlI CTYRAG 3 cut(s) 548, 981, 1084
SmoI CTYRAG 3 cut(s) 548, 981, 1084
Sse9I AATT 8 cut(s) 113, 587, 806, 913, 975, 1009, 1104, 1396
SseBI AGGCCT 1 cut(s) 1242
SsiI CCGC 3 cut(s) 9, 123, 343
SspDI GGCGCC 1 cut(s) 187
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 1242
StyD4I CCNGG 7 cut(s) 310, 541, 703, 757, 954, 1438, 1526
StyI CCWWGG 1 cut(s) 1357
TaaI ACNGT 2 cut(s) 217, 434
TaiI ACGT 5 cut(s) 39, 171, 288, 322, 1272
TaqI TCGA 5 cut(s) 73, 1212, 1435, 1536, 1635
TaqII GACCGA 1 cut(s) 315
TasI AATT 8 cut(s) 113, 587, 806, 913, 975, 1009, 1104, 1396
TauI GCSGC 2 cut(s) 11, 345
TfiI GAWTC 5 cut(s) 243, 258, 454, 1172, 1567
Tru1I TTAA 7 cut(s) 653, 725, 809, 852, 1085, 1185, 1479
Tru9I TTAA 7 cut(s) 653, 725, 809, 852, 1085, 1185, 1479
TscAI CASTG 2 cut(s) 222, 1611
TseFI GTSAC 5 cut(s) 296, 334, 364, 919, 1547
TseI GCWGC 3 cut(s) 78, 351, 776
Tsp45I GTSAC 5 cut(s) 296, 334, 364, 919, 1547
TspGWI ACGGA 2 cut(s) 1021, 1504
TspRI CASTG 2 cut(s) 222, 1611
Tth111I GACNNNGTC 1 cut(s) 925
Vha464I CTTAAG 1 cut(s) 1084
VpaK11BI GGWCC 2 cut(s) 623, 671
XagI CCTNNNNNAGG 1 cut(s) 1525
XapI RAATTY 3 cut(s) 113, 1009, 1104
XceI RCATGY 2 cut(s) 518, 842
XmnI GAANNNNTTC 3 cut(s) 321, 526, 1397
XspI CTAG 1 cut(s) 899
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.