RLG00000019944
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007866
Physical Location & Seq
Reverse (-)
58950163 .. 58957042
6880 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019944

Sequence Viewer

Length: 1653 bp
ATGGCGACGATGCCGAGCCACGGCCTGAGGCACGTGACCACGGTTCGTCCGTGGCTGCGGAATTTCCGAAGCGCGACGACGGTGGCGGCTCTGCCGTCTTCGCCTTCGGCCGAAGATTCGGAGCGGGCCGAGTCCAAGGAGAAGCCCAAGCCCAGCTCGTATTTCCCGAAGCGAGGGCAGAGTTTGGAGCTGGTGTGTGAGTCTTTGGCTTTCAAAGGGAAGGGTCTGTGTAAGGTTTCGGAGACCGGGTTCGTCGTCATGTGCGACCGGGCTTTGCCTGGTGAGCGGTTCATTGGGCGCGTGACTCGCAAGAAAGGGACCTATGCTGAGGTGACAAAGGTTCAGACACTATCTCCTCATTGGGATTTTGTGGATGCTCCTCCTCATTGGGATTTTGTGGATGCTCCTTGTGAGTATGCTTCTTACTGTGGAGGATGTAAAACGCAAAGTTTGTCCTATGAGGCGCAAATCAGAGCTAAGGAGCACCAAGTTCGTGAGTTGATAATAAATTTTGGAAAGGTTTCTCCTACAGAGCTGGAAAGCATCATGAAGCCCATTGTTCCCTGTGACATTCAGTTCCGTTACAGGAATAAGATGGAGTTCTCATTTGGTTCTCAAAAATGGTTACCTAAAGAATCATTACATGAGAGACAAGATGGAATTGAGAATTATGCCTTGGGTCTGCATGCTCCTGGCTTTTTTGATAAGGTTCTAAATGTTGACAAGTGCTTATTGCAAAGTGAGCCAGCCGATATGGTTCTTGCGGCCGTGCAACAAAGTTGGAGAGATCCACAATTAGGTCTCTCTCCTTATGATGTTTACTCTCATGCCGGATTTCTTAAGCATCTAATGCTAAGGACTGGAAGGAATGTGAAGACCGGTCTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTTACAAGCCAGAGCTACTTAAGCCTCTTCTTCAAAGGATAACAGCTATCCCCGAAGTGGTAAGCGTCATGAACAACGTAAATACGTCTGTTGGTAACACATCTCTTGGGGAGGTCGAGTACACTTTGTATGGAAAGTCTAGCATAACAGAGACCTTACGAGGGCTCACCTTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAAATTTTATATAAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGAGGGCTCAGAGATTGTTCTTGACCTTTTTTGTGGCACTGGCACCATTGGTCTGACACTTGCCAGAAGGGCCAAACATGTTTATGGCTATGAAATAATTCCTCAGGCCATTGCAGATGCTTGTCTGAATGCTGAGCTGAATGGTATCAGTAACGCCACATTTGTCCAAGGGGATCTTAACAAAATTGATGAAAGTTTTGGGAACAATTTTCCTAAGCCTGACATCGTTATTTCAGATCCCAATCGTCCAGGAATGCACATGAAGTTGATTAAGTTTCTTCTAAAGCTGAAGGCTTCACGCATTGTTTATGTATCATGTAACCCTGCCACATGTGCGCGTGACCTTGATTATCTCTGCAATGGTGTGGTAGAGAAAAACATAAAAGGCTGTTACAAGCTAAAGAGTGTGCAACCGGTAGACATGTTTCCGCACACTCCTCATATTGAGTGTGTTTGCCTCTTGGAGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

551

Amino Acids

61.32

Weight (kDa)

8.01

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 359 - 548 9.4e-16 tRNA (Uracil-5-)-methyltransferase
Methyltransf_15 PF09445 397 - 475 4.3e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 398 - 472 8.6e-13 Methyltransferase domain
Methyltransf_25 PF13649 399 - 457 2.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1220
AccBSI CCGCTC 2 cut(s) 124, 286
AccI GTMKAC 1 cut(s) 1596
AccII CGCG 3 cut(s) 74, 300, 1516
AciI CCGC 6 cut(s) 58, 86, 124, 286, 764, 1607
AclWI GGATC 3 cut(s) 782, 1359, 1409
AcoI YGGCCR 2 cut(s) 108, 765
AcsI RAATTY 3 cut(s) 61, 508, 1137
AcuI CTGAAG 1 cut(s) 1487
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 1 cut(s) 1037
AfiI CCNNNNNNNGG 5 cut(s) 20, 173, 797, 973, 1077
AflII CTTAAG 2 cut(s) 839, 935
AflIII ACRYGT 3 cut(s) 1255, 1508, 1599
AgeI ACCGGT 2 cut(s) 878, 1591
AgsI TTSAA 3 cut(s) 214, 950, 1091
AjnI CCWGG 4 cut(s) 277, 691, 1128, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 5 cut(s) 236, 643, 806, 1061, 1175
AlwI GGATC 3 cut(s) 782, 1359, 1409
AoxI GGCC 6 cut(s) 22, 108, 126, 765, 1248, 1284
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 3 cut(s) 61, 508, 1137
AsiGI ACCGGT 2 cut(s) 878, 1591
Asp700I GAANNNNTTC 2 cut(s) 520, 1275
AspLEI GCGC 4 cut(s) 74, 300, 466, 1516
AspS9I GGNCC 3 cut(s) 126, 318, 1248
AsuC2I CCSGG 2 cut(s) 247, 269
AsuHPI GGTGA 4 cut(s) 293, 343, 1075, 1118
AvaII GGWCC 1 cut(s) 318
AxyI CCTNAGG 2 cut(s) 26, 1281
BanI GGYRCC 1 cut(s) 1220
BanII GRGCYC 2 cut(s) 1083, 1187
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 3 cut(s) 90, 875, 881
Bbv12I GWGCWC 1 cut(s) 486
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 42
BccI CCATC 2 cut(s) 589, 650
BceAI ACGGC 3 cut(s) 37, 79, 752
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 4 cut(s) 279, 693, 1130, 1428
BcnI CCSGG 2 cut(s) 247, 269
BcoDI GTCTC 5 cut(s) 236, 643, 806, 1061, 1175
BfaI CTAG 1 cut(s) 1056
BfmI CTRYAG 1 cut(s) 528
BfrI CTTAAG 2 cut(s) 839, 935
BglI GCCNNNNNGGC 1 cut(s) 1247
BisI GCNGC 3 cut(s) 56, 87, 765
BlpI GCTNAGC 1 cut(s) 1311
BlsI GCNGC 3 cut(s) 57, 88, 766
Bme1390I CCNGG 6 cut(s) 247, 269, 279, 693, 1130, 1428
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 3 cut(s) 126, 318, 1248
BmiI GGNNCC 2 cut(s) 319, 1222
BmrFI CCNGG 6 cut(s) 247, 269, 279, 693, 1130, 1428
BmsI GCATC 5 cut(s) 364, 391, 552, 853, 1285
BpiI GAAGAC 3 cut(s) 90, 875, 881
Bpu10I CCTNAGC 4 cut(s) 327, 477, 854, 1392
Bpu1102I GCTNAGC 1 cut(s) 1311
BpuMI CCSGG 2 cut(s) 247, 269
BsaAI YACGTR 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 1419
BsaI GGTCTC 4 cut(s) 236, 806, 1061, 1175
BsaJI CCNNGG 6 cut(s) 19, 39, 50, 135, 675, 1345
BsaWI WCCGGW 2 cut(s) 878, 1591
BsaXI ACNNNNNCTCC 8 cut(s) 179, 209, 233, 263, 337, 367, 1019, 1049
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 173, 797, 973, 1077
Bse118I RCCGGY 2 cut(s) 878, 1591
Bse1I ACTGG 2 cut(s) 865, 1222
Bse21I CCTNAGG 2 cut(s) 26, 1281
Bse3DI GCAATG 2 cut(s) 1287, 1543
Bse8I GATNNNNATC 1 cut(s) 1419
BseBI CCWGG 4 cut(s) 279, 693, 1130, 1428
BseDI CCNNGG 6 cut(s) 19, 39, 50, 135, 675, 1345
BseGI GGATG 3 cut(s) 379, 406, 440
BseJI GATNNNNATC 1 cut(s) 1419
BseLI CCNNNNNNNGG 5 cut(s) 20, 173, 797, 973, 1077
BseMI GCAATG 2 cut(s) 1287, 1543
BseMII CTCAG 6 cut(s) 17, 318, 1186, 1200, 1295, 1302
BseNI ACTGG 2 cut(s) 865, 1222
BseRI GAGGAG 5 cut(s) 345, 369, 372, 1191, 1605
BseX3I CGGCCG 2 cut(s) 108, 765
BseXI GCAGC 1 cut(s) 42
BseYI CCCAGC 1 cut(s) 152
Bsh1236I CGCG 3 cut(s) 74, 300, 1516
Bsh1285I CGRYCG 3 cut(s) 111, 268, 768
BshFI GGCC 6 cut(s) 24, 110, 128, 767, 1250, 1286
BshNI GGYRCC 1 cut(s) 1220
BshTI ACCGGT 2 cut(s) 878, 1591
BsiEI CGRYCG 3 cut(s) 111, 268, 768
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 5 cut(s) 246, 268, 831, 879, 1592
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 5 cut(s) 20, 173, 797, 973, 1077
BsmAI GTCTC 5 cut(s) 236, 643, 806, 1061, 1175
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 2 cut(s) 1312, 1437
BsnI GGCC 6 cut(s) 24, 110, 128, 767, 1250, 1286
Bso31I GGTCTC 4 cut(s) 236, 806, 1061, 1175
Bsp1286I GDGCHC 3 cut(s) 486, 1083, 1187
Bsp143I GATC 3 cut(s) 787, 1351, 1414
Bsp1720I GCTNAGC 1 cut(s) 1311
BspACI CCGC 6 cut(s) 58, 86, 124, 286, 764, 1607
BspANI GGCC 6 cut(s) 24, 110, 128, 767, 1250, 1286
BspCNI CTCAG 6 cut(s) 18, 319, 1185, 1199, 1294, 1303
BspFNI CGCG 3 cut(s) 74, 300, 1516
BspHI TCATGA 2 cut(s) 546, 984
BspLI GGNNCC 2 cut(s) 319, 1222
BspPI GGATC 3 cut(s) 782, 1359, 1409
BspT107I GGYRCC 1 cut(s) 1220
BspTI CTTAAG 2 cut(s) 839, 935
BspTNI GGTCTC 4 cut(s) 236, 806, 1061, 1175
BsrBI CCGCTC 2 cut(s) 124, 286
BsrDI GCAATG 2 cut(s) 1287, 1543
BsrFI RCCGGY 2 cut(s) 878, 1591
BsrI ACTGG 2 cut(s) 865, 1222
BssAI RCCGGY 2 cut(s) 878, 1591
BssECI CCNNGG 6 cut(s) 19, 39, 50, 135, 675, 1345
BssMI GATC 3 cut(s) 787, 1351, 1414
BssT1I CCWWGG 3 cut(s) 135, 675, 1345
Bst2UI CCWGG 4 cut(s) 279, 693, 1130, 1428
Bst4CI ACNGT 3 cut(s) 43, 82, 428
Bst6I CTCTTC 1 cut(s) 948
BstAFI CTTAAG 2 cut(s) 839, 935
BstAPI GCANNNNNTGC 1 cut(s) 850
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 126, 687, 747
BstDEI CTNAG 9 cut(s) 26, 327, 477, 854, 1172, 1186, 1281, 1311, 1392
BstDSI CCRYGG 3 cut(s) 19, 39, 50
BstEII GGTNACC 1 cut(s) 624
BstF5I GGATG 3 cut(s) 379, 406, 440
BstFNI CGCG 3 cut(s) 74, 300, 1516
BstHHI GCGC 4 cut(s) 74, 300, 466, 1516
BstKTI GATC 3 cut(s) 790, 1354, 1417
BstMAI GTCTC 5 cut(s) 236, 643, 806, 1061, 1175
BstMBI GATC 3 cut(s) 787, 1351, 1414
BstMCI CGRYCG 3 cut(s) 111, 268, 768
BstMWI GCNNNNNNNGC 8 cut(s) 100, 283, 306, 742, 850, 937, 1247, 1511
BstNI CCWGG 4 cut(s) 279, 693, 1130, 1428
BstNSI RCATGY 4 cut(s) 689, 1259, 1512, 1603
BstPI GGTNACC 1 cut(s) 624
BstSCI CCNGG 6 cut(s) 245, 267, 277, 691, 1128, 1426
BstSFI CTRYAG 1 cut(s) 528
BstUI CGCG 3 cut(s) 74, 300, 1516
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 3 cut(s) 90, 875, 881
BstX2I RGATCY 3 cut(s) 787, 1351, 1414
BstYI RGATCY 3 cut(s) 787, 1351, 1414
BstZI CGGCCG 2 cut(s) 108, 765
Bsu36I CCTNAGG 2 cut(s) 26, 1281
BsuRI GGCC 6 cut(s) 24, 110, 128, 767, 1250, 1286
BtgI CCRYGG 3 cut(s) 19, 39, 50
BtsCI GGATG 3 cut(s) 379, 406, 440
BtsIMutI CAGTG 1 cut(s) 1215
Cac8I GCNNGC 3 cut(s) 126, 687, 747
CciI TCATGA 2 cut(s) 546, 984
CfoI GCGC 4 cut(s) 74, 300, 466, 1516
Cfr10I RCCGGY 2 cut(s) 878, 1591
Cfr13I GGNCC 3 cut(s) 126, 318, 1248
CseI GACGC 1 cut(s) 970
Csp6I GTAC 1 cut(s) 1036
CspAI ACCGGT 2 cut(s) 878, 1591
CviQI GTAC 1 cut(s) 1036
DdeI CTNAG 9 cut(s) 26, 327, 477, 854, 1172, 1186, 1281, 1311, 1392
DpnI GATC 3 cut(s) 789, 1353, 1416
DpnII GATC 3 cut(s) 787, 1351, 1414
EaeI YGGCCR 2 cut(s) 108, 765
EagI CGGCCG 2 cut(s) 108, 765
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
EclXI CGGCCG 2 cut(s) 108, 765
Eco130I CCWWGG 3 cut(s) 135, 675, 1345
Eco24I GRGCYC 2 cut(s) 1083, 1187
Eco31I GGTCTC 4 cut(s) 236, 806, 1061, 1175
Eco47I GGWCC 1 cut(s) 318
Eco52I CGGCCG 2 cut(s) 108, 765
Eco57I CTGAAG 1 cut(s) 1487
Eco72I CACGTG 1 cut(s) 34
Eco81I CCTNAGG 2 cut(s) 26, 1281
Eco91I GGTNACC 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 624
EcoRII CCWGG 4 cut(s) 277, 691, 1128, 1426
EcoT14I CCWWGG 3 cut(s) 135, 675, 1345
EcoT38I GRGCYC 2 cut(s) 1083, 1187
ErhI CCWWGG 3 cut(s) 135, 675, 1345
FalI AAGNNNNNCTT 2 cut(s) 1338, 1370
FaqI GGGAC 1 cut(s) 331
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 1596
Fnu4HI GCNGC 3 cut(s) 56, 87, 765
FokI GGATG 3 cut(s) 386, 413, 447
FriOI GRGCYC 2 cut(s) 1083, 1187
Fsp4HI GCNGC 3 cut(s) 56, 87, 765
FspBI CTAG 1 cut(s) 1056
GlaI GCGC 4 cut(s) 73, 299, 465, 1515
GluI GCNGC 3 cut(s) 56, 87, 765
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 6 cut(s) 24, 110, 128, 767, 1250, 1286
HapII CCGG 5 cut(s) 246, 268, 831, 879, 1592
HgaI GACGC 1 cut(s) 970
HhaI GCGC 4 cut(s) 74, 300, 466, 1516
Hin6I GCGC 4 cut(s) 72, 298, 464, 1514
HinP1I GCGC 4 cut(s) 72, 298, 464, 1514
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 5 cut(s) 116, 131, 200, 304, 635
HpaII CCGG 5 cut(s) 246, 268, 831, 879, 1592
HphI GGTGA 4 cut(s) 293, 343, 1075, 1118
Hpy166II GTNNAC 4 cut(s) 721, 820, 1038, 1597
Hpy188III TCNNGA 6 cut(s) 166, 494, 547, 887, 985, 1199
Hpy8I GTNNAC 4 cut(s) 721, 820, 1038, 1597
Hpy99I CGWCG 4 cut(s) 10, 79, 82, 257
HpyAV CCTTC 5 cut(s) 114, 214, 858, 1239, 1462
HpyCH4III ACNGT 3 cut(s) 43, 82, 428
HpyCH4IV ACGT 3 cut(s) 33, 993, 1001
HpyCH4V TGCA 7 cut(s) 685, 736, 772, 1292, 1435, 1536, 1588
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 283, 306, 742, 850, 937, 1247, 1511
HpyF3I CTNAG 9 cut(s) 26, 327, 477, 854, 1172, 1186, 1281, 1311, 1392
HpySE526I ACGT 3 cut(s) 33, 993, 1001
HspAI GCGC 4 cut(s) 72, 298, 464, 1514
Kzo9I GATC 3 cut(s) 787, 1351, 1414
LmnI GCTCC 7 cut(s) 121, 187, 382, 409, 481, 694, 1642
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 5 cut(s) 364, 391, 552, 853, 1285
MaeI CTAG 1 cut(s) 1056
MaeII ACGT 3 cut(s) 33, 993, 1001
MalI GATC 3 cut(s) 789, 1353, 1416
MbiI CCGCTC 2 cut(s) 124, 286
MboI GATC 3 cut(s) 787, 1351, 1414
MboII GAAGA 9 cut(s) 90, 125, 875, 886, 935, 938, 1102, 1169, 1448
MflI RGATCY 3 cut(s) 787, 1351, 1414
MhlI GDGCHC 3 cut(s) 486, 1083, 1187
MlyI GAGTC 3 cut(s) 140, 209, 298
MmeI TCCRAC 1 cut(s) 761
MroXI GAANNNNTTC 2 cut(s) 520, 1275
MseI TTAA 5 cut(s) 840, 900, 936, 1356, 1449
MslI CAYNNNNRTG 1 cut(s) 1260
MspCI CTTAAG 2 cut(s) 839, 935
MspI CCGG 5 cut(s) 246, 268, 831, 879, 1592
MspR9I CCNGG 6 cut(s) 247, 269, 279, 693, 1130, 1428
Mva1269I GAATGC 2 cut(s) 1312, 1437
MvaI CCWGG 4 cut(s) 279, 693, 1130, 1428
MvnI CGCG 3 cut(s) 74, 300, 1516
MwoI GCNNNNNNNGC 8 cut(s) 100, 283, 306, 742, 850, 937, 1247, 1511
NciI CCSGG 2 cut(s) 247, 269
NdeII GATC 3 cut(s) 787, 1351, 1414
NlaIV GGNNCC 2 cut(s) 319, 1222
NmeAIII GCCGAG 2 cut(s) 39, 154
NmuCI GTSAC 6 cut(s) 34, 301, 331, 566, 907, 1517
NspI RCATGY 4 cut(s) 689, 1259, 1512, 1603
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 2 cut(s) 546, 984
PciI ACATGT 3 cut(s) 1255, 1508, 1599
PcsI WCGNNNNNNNCGW 3 cut(s) 47, 164, 261
PctI GAATGC 2 cut(s) 1312, 1437
PdmI GAANNNNTTC 2 cut(s) 520, 1275
PfeI GAWTC 2 cut(s) 116, 635
PfoI TCCNGGA 1 cut(s) 1426
PinAI ACCGGT 2 cut(s) 878, 1591
PkrI GCNGC 3 cut(s) 57, 88, 766
PleI GAGTC 3 cut(s) 139, 208, 298
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 3 cut(s) 139, 208, 298
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 318
PscI ACATGT 3 cut(s) 1255, 1508, 1599
Psp5II RGGWCCY 1 cut(s) 318
Psp6I CCWGG 4 cut(s) 277, 691, 1128, 1426
PspCI CACGTG 1 cut(s) 34
PspEI GGTNACC 1 cut(s) 624
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 4 cut(s) 277, 691, 1128, 1426
PspN4I GGNNCC 2 cut(s) 319, 1222
PspPI GGNCC 3 cut(s) 126, 318, 1248
PspPPI RGGWCCY 1 cut(s) 318
PsuI RGATCY 3 cut(s) 787, 1351, 1414
RsaI GTAC 1 cut(s) 1037
RsaNI GTAC 1 cut(s) 1036
RseI CAYNNNNRTG 1 cut(s) 1260
SaqAI TTAA 5 cut(s) 840, 900, 936, 1356, 1449
SatI GCNGC 3 cut(s) 56, 87, 765
Sau3AI GATC 3 cut(s) 787, 1351, 1414
Sau96I GGNCC 3 cut(s) 126, 318, 1248
SchI GAGTC 3 cut(s) 140, 209, 298
ScrFI CCNGG 6 cut(s) 247, 269, 279, 693, 1130, 1428
SduI GDGCHC 3 cut(s) 486, 1083, 1187
SfaNI GCATC 5 cut(s) 364, 391, 552, 853, 1285
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 1260
SmlI CTYRAG 2 cut(s) 839, 935
SmoI CTYRAG 2 cut(s) 839, 935
SphI GCATGC 1 cut(s) 689
SsiI CCGC 6 cut(s) 58, 86, 124, 286, 764, 1607
SspMI CTAG 1 cut(s) 1056
StyD4I CCNGG 6 cut(s) 245, 267, 277, 691, 1128, 1426
StyI CCWWGG 3 cut(s) 135, 675, 1345
TaaI ACNGT 3 cut(s) 43, 82, 428
TaiI ACGT 3 cut(s) 36, 996, 1004
TaqI TCGA 1 cut(s) 1032
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 2 cut(s) 89, 767
TfiI GAWTC 2 cut(s) 116, 635
Tru1I TTAA 5 cut(s) 840, 900, 936, 1356, 1449
Tru9I TTAA 5 cut(s) 840, 900, 936, 1356, 1449
TscAI CASTG 1 cut(s) 1222
TseFI GTSAC 6 cut(s) 34, 301, 331, 566, 907, 1517
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 6 cut(s) 34, 301, 331, 566, 907, 1517
TspDTI ATGAA 6 cut(s) 280, 563, 1001, 1284, 1383, 1454
TspGWI ACGGA 2 cut(s) 39, 569
TspRI CASTG 1 cut(s) 1222
Vha464I CTTAAG 2 cut(s) 839, 935
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 3 cut(s) 61, 508, 1137
XceI RCATGY 4 cut(s) 689, 1259, 1512, 1603
XmiI GTMKAC 1 cut(s) 1596
XmnI GAANNNNTTC 2 cut(s) 520, 1275
XspI CTAG 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.