Rmu_sc0004757.1_g000012
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004757.1
OG0007866
Physical Location & Seq
Forward (+)
44817 .. 45990
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004757.1_g000012.1.cds

Sequence Viewer

Length: 591 bp
atgccgagccacggcctgaggcacgtgaccacggttcgcccgtggctgcggaatttccgaagcgcgacgacgctggcggctctgccgtcttcgccttcggccgaagattcggagcgggccgagtccaaggagaagcccaagcccaagcccagctcgtatttcccgaagcgagggcagagtttggagctggtgtgtgagtctttggctttcaaagggaagggtctgtgtaaggtttcggagaccgggttcgtcgtcatgtgcgaccgggctttgcctggggagcggttcattgggcgcgtgactcgcaagaaagggacctatgctgaggtgacaaaggtacagacactatctcctcattgggatttcgtggatgctccttgtgagtatgcttcttactgtggaggatgtaaaacgcaaagtttgtcctatgaggcgcaaatcagagctaaggagcaccaagttcgtgagttgatgataaattttggaaaggtttctcctacagagctggaaagcatcatgaagcccattgttccctgtgacatccagttccgttacaggaataaggctagtgcacaacttaaacctttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

196

Amino Acids

22.0

Weight (kDa)

9.43

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 115, 283
AccII CGCG 2 cut(s) 65, 297
AciI CCGC 4 cut(s) 49, 77, 115, 283
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 52, 478
AcvI CACGTG 1 cut(s) 25
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 2 cut(s) 11, 170
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 274
AloI GAACNNNNNNTCC 2 cut(s) 230, 262
AluBI AGCT 4 cut(s) 153, 187, 446, 505
AluI AGCT 4 cut(s) 153, 187, 446, 505
Alw21I GWGCWC 2 cut(s) 456, 574
Alw26I GTCTC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 570
AoxI GGCC 3 cut(s) 13, 99, 117
ApaLI GTGCAC 1 cut(s) 570
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 2 cut(s) 52, 478
Asp700I GAANNNNTTC 1 cut(s) 490
AspLEI GCGC 3 cut(s) 65, 297, 436
AspS9I GGNCC 2 cut(s) 117, 315
AsuC2I CCSGG 2 cut(s) 244, 266
AsuHPI GGTGA 1 cut(s) 340
AvaII GGWCC 1 cut(s) 315
AxyI CCTNAGG 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 574
BbrPI CACGTG 1 cut(s) 25
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 456, 574
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 33
BceAI ACGGC 2 cut(s) 28, 70
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BciT130I CCWGG 1 cut(s) 276
BcnI CCSGG 2 cut(s) 244, 266
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 2 cut(s) 47, 78
BlsI GCNGC 2 cut(s) 48, 79
Bme1390I CCNGG 3 cut(s) 244, 266, 276
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 117, 315
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 3 cut(s) 244, 266, 276
BmsI GCATC 2 cut(s) 361, 522
BpiI GAAGAC 1 cut(s) 81
Bpu10I CCTNAGC 2 cut(s) 324, 447
BpuMI CCSGG 2 cut(s) 244, 266
BsaAI YACGTR 1 cut(s) 25
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 5 cut(s) 10, 30, 41, 126, 275
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 230, 260, 334, 364
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 170
Bse1I ACTGG 1 cut(s) 544
Bse21I CCTNAGG 1 cut(s) 17
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 5 cut(s) 10, 30, 41, 126, 275
BseGI GGATG 3 cut(s) 376, 410, 540
BseLI CCNNNNNNNGG 2 cut(s) 11, 170
BseMII CTCAG 2 cut(s) 8, 315
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 342
BseSI GKGCMC 1 cut(s) 574
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 149
Bsh1236I CGCG 2 cut(s) 65, 297
Bsh1285I CGRYCG 2 cut(s) 102, 265
BshFI GGCC 3 cut(s) 15, 101, 119
BsiEI CGRYCG 2 cut(s) 102, 265
BsiHKAI GWGCWC 2 cut(s) 456, 574
BsiSI CCGG 2 cut(s) 243, 265
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 11, 170
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 3 cut(s) 15, 101, 119
Bso31I GGTCTC 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 456, 574
BspACI CCGC 4 cut(s) 49, 77, 115, 283
BspANI GGCC 3 cut(s) 15, 101, 119
BspCNI CTCAG 2 cut(s) 9, 316
BspFNI CGCG 2 cut(s) 65, 297
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 316
BspTNI GGTCTC 1 cut(s) 233
BsrBI CCGCTC 2 cut(s) 115, 283
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 5 cut(s) 10, 30, 41, 126, 275
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 34, 398
BstBAI YACGTR 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 75, 117
BstDEI CTNAG 3 cut(s) 17, 324, 447
BstDSI CCRYGG 3 cut(s) 10, 30, 41
BstF5I GGATG 3 cut(s) 376, 410, 540
BstFNI CGCG 2 cut(s) 65, 297
BstHHI GCGC 3 cut(s) 65, 297, 436
BstMAI GTCTC 1 cut(s) 233
BstMCI CGRYCG 2 cut(s) 102, 265
BstMWI GCNNNNNNNGC 3 cut(s) 91, 280, 303
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 3 cut(s) 242, 264, 274
BstSFI CTRYAG 1 cut(s) 498
BstSLI GKGCMC 1 cut(s) 574
BstUI CGCG 2 cut(s) 65, 297
BstV1I GCAGC 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 81
BstZI CGGCCG 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 3 cut(s) 15, 101, 119
BtgI CCRYGG 3 cut(s) 10, 30, 41
BtsCI GGATG 3 cut(s) 376, 410, 540
Cac8I GCNNGC 2 cut(s) 75, 117
CciI TCATGA 1 cut(s) 516
CfoI GCGC 3 cut(s) 65, 297, 436
Cfr13I GGNCC 2 cut(s) 117, 315
CseI GACGC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 338
CviAII CATG 2 cut(s) 256, 517
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 3 cut(s) 17, 324, 447
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 315
Eco52I CGGCCG 1 cut(s) 99
Eco72I CACGTG 1 cut(s) 25
Eco81I CCTNAGG 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 2 cut(s) 259, 520
FaiI YATR 5 cut(s) 257, 321, 387, 429, 518
FaqI GGGAC 1 cut(s) 328
FatI CATG 2 cut(s) 255, 516
FauI CCCGC 1 cut(s) 108
Fnu4HI GCNGC 2 cut(s) 47, 78
FokI GGATG 3 cut(s) 383, 417, 527
Fsp4HI GCNGC 2 cut(s) 47, 78
FspBI CTAG 1 cut(s) 567
GlaI GCGC 3 cut(s) 64, 296, 435
GluI GCNGC 2 cut(s) 47, 78
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 3 cut(s) 15, 101, 119
HapII CCGG 2 cut(s) 243, 265
HgaI GACGC 1 cut(s) 79
HhaI GCGC 3 cut(s) 65, 297, 436
Hin1II CATG 2 cut(s) 259, 520
Hin6I GCGC 3 cut(s) 63, 295, 434
HinP1I GCGC 3 cut(s) 63, 295, 434
HinfI GANTC 4 cut(s) 107, 122, 197, 301
HpaII CCGG 2 cut(s) 243, 265
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 572
Hpy188I TCNGA 4 cut(s) 59, 112, 238, 443
Hpy188III TCNNGA 3 cut(s) 163, 464, 517
Hpy8I GTNNAC 1 cut(s) 572
Hpy99I CGWCG 3 cut(s) 70, 73, 254
HpyAV CCTTC 2 cut(s) 105, 211
HpyCH4III ACNGT 2 cut(s) 34, 398
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 280, 303
HpyF3I CTNAG 3 cut(s) 17, 324, 447
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 2 cut(s) 259, 520
HspAI GCGC 3 cut(s) 63, 295, 434
LmnI GCTCC 5 cut(s) 112, 184, 280, 379, 451
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 2 cut(s) 361, 522
MaeI CTAG 1 cut(s) 567
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 5 cut(s) 25, 298, 328, 536, 551
MbiI CCGCTC 2 cut(s) 115, 283
MboII GAAGA 2 cut(s) 81, 116
MhlI GDGCHC 2 cut(s) 456, 574
MluCI AATT 2 cut(s) 52, 478
MlyI GAGTC 3 cut(s) 131, 206, 295
MnlI CCTC 6 cut(s) 12, 164, 319, 363, 395, 424
MroXI GAANNNNTTC 1 cut(s) 490
MseI TTAA 2 cut(s) 579, 589
MspI CCGG 2 cut(s) 243, 265
MspR9I CCNGG 3 cut(s) 244, 266, 276
MvaI CCWGG 1 cut(s) 276
MvnI CGCG 2 cut(s) 65, 297
MwoI GCNNNNNNNGC 3 cut(s) 91, 280, 303
NciI CCSGG 2 cut(s) 244, 266
NlaIII CATG 2 cut(s) 259, 520
NlaIV GGNNCC 1 cut(s) 316
NmeAIII GCCGAG 2 cut(s) 30, 145
NmuCI GTSAC 4 cut(s) 25, 298, 328, 536
PagI TCATGA 1 cut(s) 516
PcsI WCGNNNNNNNCGW 3 cut(s) 38, 161, 258
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 48, 79
PleI GAGTC 3 cut(s) 130, 205, 295
PmaCI CACGTG 1 cut(s) 25
PmlI CACGTG 1 cut(s) 25
PpsI GAGTC 3 cut(s) 130, 205, 295
Ppu21I YACGTR 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 274
PspCI CACGTG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 117, 315
PspPPI RGGWCCY 1 cut(s) 315
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SaqAI TTAA 2 cut(s) 579, 589
SatI GCNGC 2 cut(s) 47, 78
Sau96I GGNCC 2 cut(s) 117, 315
SchI GAGTC 3 cut(s) 131, 206, 295
ScrFI CCNGG 3 cut(s) 244, 266, 276
SduI GDGCHC 2 cut(s) 456, 574
SfaNI GCATC 2 cut(s) 361, 522
SfcI CTRYAG 1 cut(s) 498
SinI GGWCC 1 cut(s) 315
Sse9I AATT 2 cut(s) 52, 478
SsiI CCGC 4 cut(s) 49, 77, 115, 283
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 3 cut(s) 242, 264, 274
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 34, 398
TaiI ACGT 1 cut(s) 27
TasI AATT 2 cut(s) 52, 478
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 579, 589
Tru9I TTAA 2 cut(s) 579, 589
TseFI GTSAC 4 cut(s) 25, 298, 328, 536
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 4 cut(s) 25, 298, 328, 536
TspDTI ATGAA 2 cut(s) 277, 533
TspGWI ACGGA 1 cut(s) 539
VneI GTGCAC 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 2 cut(s) 52, 478
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.