Rorug02G0369500
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007866
Physical Location & Seq
Forward (+)
47117851 .. 47121150
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0369500.1

Sequence Viewer

Length: 570 bp
ATGTTGGATGAGGATGCTGCTTCTCGGCTAGTTCCTAAACTTTTGTTTTGTAAATATGCTTGCATCCCTCTGGTTGGGCATAGTGGTGGCTTGTTGCTGTTATGGAATGATAATAAATTTAATATTAATGTACTTGATCAACATCCTTATTTTATTCATACCCGTTGCACTAATTTGTTGAATGGTACTGTTTGGTTTACCACGTTTCTGTATATGTATCCTCAGAAAGAGTTGCAGAATGGATTGTGGCTTGATTTATTAAATCTTCATGTTCCTTCTTTTGAGGCATGGATTATTATTGGGGATTTTAATTGTATTCTGAATAGTGCTGAAAAGCGGGGTGGGCTTCGTGTTACAAATCGTTATATGGTGCAATTTCAGGACTTTTTGAATTGTGCTGGATTGATTTCTTTGCCTTATACTGGTAGTGCGTTTACTTGGACTAATAAGCAACAGGCTGATATTAAGGAATGGTTGGATCGAGCTGTCGCAAATCCTGTTGCTTATACTTTGTTTCCATTCATGACTCTTAAGACCATTATCTCGGCAATAATGAGAAAAAGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

189

Amino Acids

21.72

Weight (kDa)

8.27

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 23 - 166 1.1e-06 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 337
AclWI GGATC 1 cut(s) 486
AcsI RAATTY 1 cut(s) 116
AfaI GTAC 2 cut(s) 132, 187
AfiI CCNNNNNNNGG 2 cut(s) 74, 422
AflII CTTAAG 1 cut(s) 530
AgsI TTSAA 2 cut(s) 181, 391
AluBI AGCT 2 cut(s) 485, 564
AluI AGCT 2 cut(s) 485, 564
AlwI GGATC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 116
AseI ATTAAT 1 cut(s) 126
BbvI GCAGC 1 cut(s) 4
BciVI GTATCC 1 cut(s) 228
BclI TGATCA 1 cut(s) 136
BfaI CTAG 1 cut(s) 29
BfrI CTTAAG 1 cut(s) 530
BfuI GTATCC 1 cut(s) 228
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
BmsI GCATC 2 cut(s) 4, 72
BsaBI GATNNNNATC 1 cut(s) 141
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 422
Bse1I ACTGG 1 cut(s) 427
Bse8I GATNNNNATC 1 cut(s) 141
BseGI GGATG 4 cut(s) 13, 19, 63, 142
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 74, 422
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 427
BseXI GCAGC 1 cut(s) 4
BslI CCNNNNNNNGG 2 cut(s) 74, 422
Bsp143I GATC 2 cut(s) 136, 478
BspACI CCGC 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 522
BspPI GGATC 1 cut(s) 486
BspTI CTTAAG 1 cut(s) 530
BsrI ACTGG 1 cut(s) 427
BssMI GATC 2 cut(s) 136, 478
Bst4CI ACNGT 1 cut(s) 190
BstAFI CTTAAG 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 4 cut(s) 13, 19, 63, 142
BstKTI GATC 2 cut(s) 139, 481
BstMBI GATC 2 cut(s) 136, 478
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 4
BsuI GTATCC 1 cut(s) 228
BtsCI GGATG 4 cut(s) 13, 19, 63, 142
Cac8I GCNNGC 1 cut(s) 61
CciI TCATGA 1 cut(s) 522
Csp6I GTAC 2 cut(s) 131, 186
CviAII CATG 3 cut(s) 269, 288, 523
CviJI RGCY 7 cut(s) 28, 90, 250, 346, 458, 485, 564
CviKI_1 RGCY 7 cut(s) 28, 90, 250, 346, 458, 485, 564
CviQI GTAC 2 cut(s) 131, 186
DdeI CTNAG 1 cut(s) 222
DpnI GATC 2 cut(s) 138, 480
DpnII GATC 2 cut(s) 136, 478
FaeI CATG 3 cut(s) 272, 291, 526
FatI CATG 3 cut(s) 268, 287, 522
FauI CCCGC 1 cut(s) 330
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 20, 26, 50, 129
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 29
GluI GCNGC 1 cut(s) 18
Hin1II CATG 3 cut(s) 272, 291, 526
HindIII AAGCTT 1 cut(s) 562
HinfI GANTC 1 cut(s) 526
Hpy166II GTNNAC 2 cut(s) 198, 435
Hpy188I TCNGA 2 cut(s) 225, 321
Hpy188III TCNNGA 2 cut(s) 380, 523
Hpy8I GTNNAC 2 cut(s) 198, 435
HpyAV CCTTC 1 cut(s) 285
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 4 cut(s) 63, 168, 235, 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 203
Hsp92II CATG 3 cut(s) 272, 291, 526
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 478
LpnPI CCDG 6 cut(s) 56, 365, 384, 408, 440, 510
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 2 cut(s) 4, 72
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 138, 480
MboI GATC 2 cut(s) 136, 478
MboII GAAGA 1 cut(s) 257
MluCI AATT 5 cut(s) 116, 172, 310, 374, 391
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 4 cut(s) 4, 78, 231, 277
MseI TTAA 6 cut(s) 120, 126, 260, 309, 465, 531
MslI CAYNNNNRTG 1 cut(s) 84
MspCI CTTAAG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 343
NdeII GATC 2 cut(s) 136, 478
NlaIII CATG 3 cut(s) 272, 291, 526
NmeAIII GCCGAG 2 cut(s) 4, 524
PagI TCATGA 1 cut(s) 522
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 126
RsaI GTAC 2 cut(s) 132, 187
RsaNI GTAC 2 cut(s) 131, 186
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 6 cut(s) 120, 126, 260, 309, 465, 531
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 2 cut(s) 136, 478
SchI GAGTC 1 cut(s) 520
SetI ASST 3 cut(s) 206, 487, 566
SfaNI GCATC 2 cut(s) 4, 72
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 5 cut(s) 116, 172, 310, 374, 391
SsiI CCGC 1 cut(s) 337
SspI AATATT 1 cut(s) 124
SspMI CTAG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 206
TaqI TCGA 1 cut(s) 481
TasI AATT 5 cut(s) 116, 172, 310, 374, 391
TatI WGTACW 1 cut(s) 130
Tru1I TTAA 6 cut(s) 120, 126, 260, 309, 465, 531
Tru9I TTAA 6 cut(s) 120, 126, 260, 309, 465, 531
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 3 cut(s) 146, 257, 511
Vha464I CTTAAG 1 cut(s) 530
VspI ATTAAT 1 cut(s) 126
XapI RAATTY 1 cut(s) 116
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.