Rw2G034590
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007866
Physical Location & Seq
Reverse (-)
56921589 .. 56928858
7270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G034590.1

Sequence Viewer

Length: 1632 bp
ATGGCGACGATGCCGAGCCACGGCCTGAGGCACGTGACCACGGTTCGCCCTTGGCTGCGGAATTTCCGAAGCGCGACGACGCTGGCGGCTCTGCCGTCTTCGCCTTCGGCCGAAGATTCGGAGCGGGCCGAGTCCAAGGAGAAGCCCAAGCCCAAGGCCAGCTCGTATTTCCCGAAGCGAGGGCAGAGTTTGGAGCTGGTGTGTGAGTCTTTGGCTTTCAAAGGGAAGGGTCTGTGTAAGGTTTCGGAGACCGGGTTCGTCGTCATGTGCGACCGGGCTTTGCCCGGGGAGCGGTTCATTGGGCGCGTGACTCGCAAGAAAGGGACCTATGCTGAGGTGACAAAGGTACAGACACTATCTCCTCATTGGGATTTCGTGGATGCTCCTTGTGAGTATGCTTCTTACTGTGGAGGATGTAAAACGCAAAGTTTGTCCTATGAGGCGCAAATCAGAGCTAAGGAGCACCAAGTTCGTGAGTTGATGATAAATTTTGGAAAGGTTTCTCCTACAGAGCTGGAAAGCATCATGAAGCCCATTGTTCCCTGTGACATCCAGTTCCGTTACAGGAATAAGATGGAGTTCTCATTTGGTTCTCAAAAATGGTTACCTAAAGAATCATTACACGAGAGACAAGATGGAATTGAGAATTATGCCTTGGGTCTGCATGCTCCTGGCTTTTTTGATAAGGTTCTAAATGTTGACAAGTGCTTATTGCAAAGTGAGCCTGCCGACATGGTTCTTGCCGCCGTGCAACAAAGTTGGAGAGATCCACAATTAGGTCTCTCTCCTTATGATGTTTACTCTCATGCCGGATTTCTTAAGCATCTAATGCTAAGAACTGGAAGGAATGTGAAGACCGGTCTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTTACAAGCCAGAGCTACTTAAGCCTCTTCTTCAAAGGATAACAGCTATCCCCGAAGTGGTAAGCGTCATGAACAATGTAAATACGTCTGTTGGTAACACGTCTCTTGGGGAGGTCGAGTACACTTTGTATGGAAAGTCTAGCATAACAGAGACCTTACGAGGGCTCACCTTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAGATTTTATATAAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGAGGGCTCAGAGATTGTTCTTGACCTGTTTTGTGGGACTGGCACCATTGGTCTGACACTTGCCAGAAGGGCCAAACATGTTTATGGCTATGAAATAATTCCTCAGGCCATTGCAGATGCTTGTCTGAATGCTGAGCTGAATGGTATCCGTAACGCTACATTTGTCCAAGGGGATCTTAACAAAATTGATGAGAGTTTTGGGAACAATTTTCCTAAGCCTGACATCGTTATTTCAGATCCCAATCGTCCAGGAATGCACATGAAGTTGATTAAGTTTCTTCTAAAGCTGAAGGCTTCACGCATTGTTTATGTATCATGTAACCCTGCCACATGTGCACGTGACCTTGATTATCTCTGCAATGGTGTGGTAGAGAAAAACATAAAAGGCTGTTACAAGCTAAAGAGTGTGCAACCAGTAGACATGTTTCCGCACACTCCTCATATTGAGTGTGTTTGCCTCTTGGAGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

543

Amino Acids

60.56

Weight (kDa)

8.5

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 352 - 541 1.3e-15 tRNA (Uracil-5-)-methyltransferase
Methyltransf_15 PF09445 390 - 468 8e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 391 - 465 1.7e-12 Methyltransferase domain
Methyltransf_25 PF13649 392 - 450 2.2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1199
AccBSI CCGCTC 2 cut(s) 124, 292
AccI GTMKAC 1 cut(s) 1575
AccII CGCG 2 cut(s) 74, 306
AciI CCGC 6 cut(s) 58, 86, 124, 292, 744, 1586
AclWI GGATC 3 cut(s) 761, 1338, 1388
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 2 cut(s) 61, 487
AcuI CTGAAG 1 cut(s) 1466
AcvI CACGTG 2 cut(s) 34, 1496
AfaI GTAC 2 cut(s) 348, 1016
AfiI CCNNNNNNNGG 6 cut(s) 20, 179, 291, 776, 952, 1056
AflII CTTAAG 2 cut(s) 818, 914
AflIII ACRYGT 4 cut(s) 993, 1234, 1487, 1578
AgeI ACCGGT 1 cut(s) 857
AgsI TTSAA 3 cut(s) 220, 929, 1070
AjiI CACGTC 1 cut(s) 996
AjnI CCWGG 3 cut(s) 670, 1107, 1405
AjuI GAANNNNNNNTTGG 2 cut(s) 638, 670
AloI GAACNNNNNNTCC 2 cut(s) 239, 271
Alw21I GWGCWC 2 cut(s) 465, 1495
Alw26I GTCTC 6 cut(s) 242, 622, 785, 1002, 1040, 1154
Alw44I GTGCAC 1 cut(s) 1491
AlwI GGATC 3 cut(s) 761, 1338, 1388
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 6 cut(s) 22, 108, 126, 156, 1227, 1263
ApaLI GTGCAC 1 cut(s) 1491
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 2 cut(s) 61, 487
AsiGI ACCGGT 1 cut(s) 857
Asp700I GAANNNNTTC 2 cut(s) 499, 1254
AspLEI GCGC 3 cut(s) 74, 306, 445
AspS9I GGNCC 3 cut(s) 126, 324, 1227
AsuC2I CCSGG 4 cut(s) 253, 275, 285, 286
AsuHPI GGTGA 3 cut(s) 349, 1054, 1097
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 324
AxyI CCTNAGG 2 cut(s) 26, 1260
BaeGI GKGCMC 1 cut(s) 1495
BanI GGYRCC 1 cut(s) 1199
BanII GRGCYC 2 cut(s) 1062, 1166
BauI CACGAG 1 cut(s) 623
BbrPI CACGTG 2 cut(s) 34, 1496
BbsI GAAGAC 3 cut(s) 90, 854, 860
Bbv12I GWGCWC 2 cut(s) 465, 1495
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 42
BccI CCATC 2 cut(s) 568, 629
BceAI ACGGC 3 cut(s) 37, 79, 731
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 3 cut(s) 672, 1109, 1407
BciVI GTATCC 1 cut(s) 1313
BcnI CCSGG 4 cut(s) 253, 275, 285, 286
BcoDI GTCTC 6 cut(s) 242, 622, 785, 1002, 1040, 1154
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 2 cut(s) 818, 914
BfuI GTATCC 1 cut(s) 1313
BglI GCCNNNNNGGC 1 cut(s) 1226
BisI GCNGC 3 cut(s) 56, 87, 744
BlpI GCTNAGC 1 cut(s) 1290
BlsI GCNGC 3 cut(s) 57, 88, 745
Bme1390I CCNGG 7 cut(s) 253, 275, 285, 286, 672, 1109, 1407
Bme18I GGWCC 1 cut(s) 324
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 996
BmgT120I GGNCC 3 cut(s) 126, 324, 1227
BmiI GGNNCC 2 cut(s) 325, 1201
BmrFI CCNGG 7 cut(s) 253, 275, 285, 286, 672, 1109, 1407
BmsI GCATC 4 cut(s) 370, 531, 832, 1264
BpiI GAAGAC 3 cut(s) 90, 854, 860
Bpu10I CCTNAGC 3 cut(s) 333, 456, 1371
Bpu1102I GCTNAGC 1 cut(s) 1290
BpuMI CCSGG 4 cut(s) 253, 275, 285, 286
BsaAI YACGTR 2 cut(s) 34, 1496
BsaBI GATNNNNATC 1 cut(s) 1398
BsaI GGTCTC 4 cut(s) 242, 785, 1040, 1154
BsaJI CCNNGG 9 cut(s) 19, 39, 50, 135, 153, 284, 285, 654, 1324
BsaWI WCCGGW 1 cut(s) 857
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 239, 269, 343, 373, 998, 1028
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 179, 291, 776, 952, 1056
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 4 cut(s) 553, 844, 1201, 1571
Bse21I CCTNAGG 2 cut(s) 26, 1260
Bse3DI GCAATG 2 cut(s) 1266, 1522
Bse8I GATNNNNATC 1 cut(s) 1398
BseBI CCWGG 3 cut(s) 672, 1109, 1407
BseDI CCNNGG 9 cut(s) 19, 39, 50, 135, 153, 284, 285, 654, 1324
BseGI GGATG 3 cut(s) 385, 419, 549
BseJI GATNNNNATC 1 cut(s) 1398
BseLI CCNNNNNNNGG 6 cut(s) 20, 179, 291, 776, 952, 1056
BseMI GCAATG 2 cut(s) 1266, 1522
BseMII CTCAG 6 cut(s) 17, 324, 1165, 1179, 1274, 1281
BseNI ACTGG 4 cut(s) 553, 844, 1201, 1571
BseRI GAGGAG 3 cut(s) 351, 1170, 1584
BseSI GKGCMC 1 cut(s) 1495
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 42
Bsh1236I CGCG 2 cut(s) 74, 306
Bsh1285I CGRYCG 2 cut(s) 111, 274
BshFI GGCC 6 cut(s) 24, 110, 128, 158, 1229, 1265
BshNI GGYRCC 1 cut(s) 1199
BshTI ACCGGT 1 cut(s) 857
BsiEI CGRYCG 2 cut(s) 111, 274
BsiHKAI GWGCWC 2 cut(s) 465, 1495
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 5 cut(s) 252, 274, 285, 810, 858
BslFI GGGAC 2 cut(s) 337, 1207
BslI CCNNNNNNNGG 6 cut(s) 20, 179, 291, 776, 952, 1056
BsmAI GTCTC 6 cut(s) 242, 622, 785, 1002, 1040, 1154
BsmBI CGTCTC 1 cut(s) 1002
BsmFI GGGAC 2 cut(s) 337, 1207
BsmI GAATGC 2 cut(s) 1291, 1416
BsnI GGCC 6 cut(s) 24, 110, 128, 158, 1229, 1265
Bso31I GGTCTC 4 cut(s) 242, 785, 1040, 1154
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 4 cut(s) 465, 1062, 1166, 1495
Bsp143I GATC 3 cut(s) 766, 1330, 1393
Bsp1720I GCTNAGC 1 cut(s) 1290
BspACI CCGC 6 cut(s) 58, 86, 124, 292, 744, 1586
BspANI GGCC 6 cut(s) 24, 110, 128, 158, 1229, 1265
BspCNI CTCAG 6 cut(s) 18, 325, 1164, 1178, 1273, 1282
BspFNI CGCG 2 cut(s) 74, 306
BspHI TCATGA 2 cut(s) 525, 963
BspLI GGNNCC 2 cut(s) 325, 1201
BspPI GGATC 3 cut(s) 761, 1338, 1388
BspT107I GGYRCC 1 cut(s) 1199
BspTI CTTAAG 2 cut(s) 818, 914
BspTNI GGTCTC 4 cut(s) 242, 785, 1040, 1154
BsrBI CCGCTC 2 cut(s) 124, 292
BsrDI GCAATG 2 cut(s) 1266, 1522
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 4 cut(s) 553, 844, 1201, 1571
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 9 cut(s) 19, 39, 50, 135, 153, 284, 285, 654, 1324
BssMI GATC 3 cut(s) 766, 1330, 1393
BssSI CACGAG 1 cut(s) 623
BssT1I CCWWGG 5 cut(s) 50, 135, 153, 654, 1324
Bst2BI CACGAG 1 cut(s) 623
Bst2UI CCWGG 3 cut(s) 672, 1109, 1407
Bst4CI ACNGT 2 cut(s) 43, 407
Bst6I CTCTTC 1 cut(s) 927
BstAFI CTTAAG 2 cut(s) 818, 914
BstAPI GCANNNNNTGC 1 cut(s) 829
BstBAI YACGTR 2 cut(s) 34, 1496
BstC8I GCNNGC 5 cut(s) 84, 126, 160, 666, 726
BstDEI CTNAG 9 cut(s) 26, 333, 456, 833, 1151, 1165, 1260, 1290, 1371
BstDSI CCRYGG 2 cut(s) 19, 39
BstEII GGTNACC 1 cut(s) 603
BstF5I GGATG 3 cut(s) 385, 419, 549
BstFNI CGCG 2 cut(s) 74, 306
BstHHI GCGC 3 cut(s) 74, 306, 445
BstKTI GATC 3 cut(s) 769, 1333, 1396
BstMAI GTCTC 6 cut(s) 242, 622, 785, 1002, 1040, 1154
BstMBI GATC 3 cut(s) 766, 1330, 1393
BstMCI CGRYCG 2 cut(s) 111, 274
BstMWI GCNNNNNNNGC 8 cut(s) 100, 289, 312, 721, 829, 916, 1226, 1490
BstNI CCWGG 3 cut(s) 672, 1109, 1407
BstNSI RCATGY 4 cut(s) 668, 1238, 1491, 1582
BstPI GGTNACC 1 cut(s) 603
BstSCI CCNGG 7 cut(s) 251, 273, 283, 284, 670, 1107, 1405
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 1495
BstUI CGCG 2 cut(s) 74, 306
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 3 cut(s) 90, 854, 860
BstX2I RGATCY 3 cut(s) 766, 1330, 1393
BstYI RGATCY 3 cut(s) 766, 1330, 1393
BstZI CGGCCG 1 cut(s) 108
Bsu36I CCTNAGG 2 cut(s) 26, 1260
BsuI GTATCC 1 cut(s) 1313
BsuRI GGCC 6 cut(s) 24, 110, 128, 158, 1229, 1265
BtgI CCRYGG 2 cut(s) 19, 39
BtrI CACGTC 1 cut(s) 996
BtsCI GGATG 3 cut(s) 385, 419, 549
Cac8I GCNNGC 5 cut(s) 84, 126, 160, 666, 726
CciI TCATGA 2 cut(s) 525, 963
CfoI GCGC 3 cut(s) 74, 306, 445
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 3 cut(s) 126, 324, 1227
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 2 cut(s) 88, 949
Csp6I GTAC 2 cut(s) 347, 1015
CspAI ACCGGT 1 cut(s) 857
CviQI GTAC 2 cut(s) 347, 1015
DdeI CTNAG 9 cut(s) 26, 333, 456, 833, 1151, 1165, 1260, 1290, 1371
DpnI GATC 3 cut(s) 768, 1332, 1395
DpnII GATC 3 cut(s) 766, 1330, 1393
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 5 cut(s) 50, 135, 153, 654, 1324
Eco24I GRGCYC 2 cut(s) 1062, 1166
Eco31I GGTCTC 4 cut(s) 242, 785, 1040, 1154
Eco47I GGWCC 1 cut(s) 324
Eco52I CGGCCG 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 1466
Eco72I CACGTG 2 cut(s) 34, 1496
Eco81I CCTNAGG 2 cut(s) 26, 1260
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 324
EcoO65I GGTNACC 1 cut(s) 603
EcoRII CCWGG 3 cut(s) 670, 1107, 1405
EcoT14I CCWWGG 5 cut(s) 50, 135, 153, 654, 1324
EcoT38I GRGCYC 2 cut(s) 1062, 1166
ErhI CCWWGG 5 cut(s) 50, 135, 153, 654, 1324
Esp3I CGTCTC 1 cut(s) 1002
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 2 cut(s) 337, 1207
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 1575
Fnu4HI GCNGC 3 cut(s) 56, 87, 744
FokI GGATG 3 cut(s) 392, 426, 536
FriOI GRGCYC 2 cut(s) 1062, 1166
Fsp4HI GCNGC 3 cut(s) 56, 87, 744
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 3 cut(s) 73, 305, 444
GluI GCNGC 3 cut(s) 56, 87, 744
HaeIII GGCC 6 cut(s) 24, 110, 128, 158, 1229, 1265
HapII CCGG 5 cut(s) 252, 274, 285, 810, 858
HgaI GACGC 2 cut(s) 88, 949
HhaI GCGC 3 cut(s) 74, 306, 445
Hin6I GCGC 3 cut(s) 72, 304, 443
HinP1I GCGC 3 cut(s) 72, 304, 443
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HinfI GANTC 5 cut(s) 116, 131, 206, 310, 614
HpaII CCGG 5 cut(s) 252, 274, 285, 810, 858
HphI GGTGA 3 cut(s) 349, 1054, 1097
Hpy166II GTNNAC 5 cut(s) 700, 799, 1017, 1493, 1576
Hpy188I TCNGA 9 cut(s) 68, 121, 247, 452, 1154, 1168, 1212, 1284, 1393
Hpy188III TCNNGA 6 cut(s) 172, 473, 526, 866, 964, 1178
Hpy8I GTNNAC 5 cut(s) 700, 799, 1017, 1493, 1576
Hpy99I CGWCG 4 cut(s) 10, 79, 82, 263
HpyAV CCTTC 5 cut(s) 114, 220, 837, 1218, 1441
HpyCH4III ACNGT 2 cut(s) 43, 407
HpyCH4IV ACGT 4 cut(s) 33, 980, 995, 1495
HpyCH4V TGCA 8 cut(s) 664, 715, 751, 1271, 1414, 1493, 1515, 1567
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 289, 312, 721, 829, 916, 1226, 1490
HpyF3I CTNAG 9 cut(s) 26, 333, 456, 833, 1151, 1165, 1260, 1290, 1371
HpySE526I ACGT 4 cut(s) 33, 980, 995, 1495
HspAI GCGC 3 cut(s) 72, 304, 443
Kzo9I GATC 3 cut(s) 766, 1330, 1393
LmnI GCTCC 7 cut(s) 121, 193, 289, 388, 460, 673, 1621
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 4 cut(s) 370, 531, 832, 1264
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 4 cut(s) 33, 980, 995, 1495
MalI GATC 3 cut(s) 768, 1332, 1395
MbiI CCGCTC 2 cut(s) 124, 292
MboI GATC 3 cut(s) 766, 1330, 1393
MboII GAAGA 9 cut(s) 90, 125, 854, 865, 914, 917, 1081, 1148, 1427
MflI RGATCY 3 cut(s) 766, 1330, 1393
MhlI GDGCHC 4 cut(s) 465, 1062, 1166, 1495
MluCI AATT 9 cut(s) 61, 487, 639, 646, 773, 880, 1254, 1341, 1363
MlyI GAGTC 3 cut(s) 140, 215, 304
MmeI TCCRAC 1 cut(s) 740
MroXI GAANNNNTTC 2 cut(s) 499, 1254
MseI TTAA 5 cut(s) 819, 879, 915, 1335, 1428
MslI CAYNNNNRTG 1 cut(s) 1239
MspCI CTTAAG 2 cut(s) 818, 914
MspI CCGG 5 cut(s) 252, 274, 285, 810, 858
MspR9I CCNGG 7 cut(s) 253, 275, 285, 286, 672, 1109, 1407
Mva1269I GAATGC 2 cut(s) 1291, 1416
MvaI CCWGG 3 cut(s) 672, 1109, 1407
MvnI CGCG 2 cut(s) 74, 306
MwoI GCNNNNNNNGC 8 cut(s) 100, 289, 312, 721, 829, 916, 1226, 1490
NciI CCSGG 4 cut(s) 253, 275, 285, 286
NdeII GATC 3 cut(s) 766, 1330, 1393
NlaIV GGNNCC 2 cut(s) 325, 1201
NmeAIII GCCGAG 2 cut(s) 39, 154
NmuCI GTSAC 6 cut(s) 34, 307, 337, 545, 886, 1496
NspI RCATGY 4 cut(s) 668, 1238, 1491, 1582
PaeI GCATGC 1 cut(s) 668
PagI TCATGA 2 cut(s) 525, 963
PciI ACATGT 3 cut(s) 1234, 1487, 1578
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 267
PctI GAATGC 2 cut(s) 1291, 1416
PdmI GAANNNNTTC 2 cut(s) 499, 1254
PfeI GAWTC 2 cut(s) 116, 614
PfoI TCCNGGA 1 cut(s) 1405
PinAI ACCGGT 1 cut(s) 857
PkrI GCNGC 3 cut(s) 57, 88, 745
PleI GAGTC 3 cut(s) 139, 214, 304
PmaCI CACGTG 2 cut(s) 34, 1496
PmlI CACGTG 2 cut(s) 34, 1496
PpsI GAGTC 3 cut(s) 139, 214, 304
Ppu21I YACGTR 2 cut(s) 34, 1496
PpuMI RGGWCCY 1 cut(s) 324
PscI ACATGT 3 cut(s) 1234, 1487, 1578
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 3 cut(s) 670, 1107, 1405
PspCI CACGTG 2 cut(s) 34, 1496
PspEI GGTNACC 1 cut(s) 603
PspGI CCWGG 3 cut(s) 670, 1107, 1405
PspN4I GGNNCC 2 cut(s) 325, 1201
PspPI GGNCC 3 cut(s) 126, 324, 1227
PspPPI RGGWCCY 1 cut(s) 324
PsuI RGATCY 3 cut(s) 766, 1330, 1393
RsaI GTAC 2 cut(s) 348, 1016
RsaNI GTAC 2 cut(s) 347, 1015
RseI CAYNNNNRTG 1 cut(s) 1239
SaqAI TTAA 5 cut(s) 819, 879, 915, 1335, 1428
SatI GCNGC 3 cut(s) 56, 87, 744
Sau3AI GATC 3 cut(s) 766, 1330, 1393
Sau96I GGNCC 3 cut(s) 126, 324, 1227
SchI GAGTC 3 cut(s) 140, 215, 304
ScrFI CCNGG 7 cut(s) 253, 275, 285, 286, 672, 1109, 1407
SduI GDGCHC 4 cut(s) 465, 1062, 1166, 1495
SfaNI GCATC 4 cut(s) 370, 531, 832, 1264
SfcI CTRYAG 1 cut(s) 507
SinI GGWCC 1 cut(s) 324
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 1239
SmlI CTYRAG 2 cut(s) 818, 914
SmoI CTYRAG 2 cut(s) 818, 914
SphI GCATGC 1 cut(s) 668
Sse9I AATT 9 cut(s) 61, 487, 639, 646, 773, 880, 1254, 1341, 1363
SsiI CCGC 6 cut(s) 58, 86, 124, 292, 744, 1586
SspMI CTAG 1 cut(s) 1035
StyD4I CCNGG 7 cut(s) 251, 273, 283, 284, 670, 1107, 1405
StyI CCWWGG 5 cut(s) 50, 135, 153, 654, 1324
TaaI ACNGT 2 cut(s) 43, 407
TaiI ACGT 4 cut(s) 36, 983, 998, 1498
TaqI TCGA 1 cut(s) 1011
TasI AATT 9 cut(s) 61, 487, 639, 646, 773, 880, 1254, 1341, 1363
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 2 cut(s) 89, 746
TfiI GAWTC 2 cut(s) 116, 614
Tru1I TTAA 5 cut(s) 819, 879, 915, 1335, 1428
Tru9I TTAA 5 cut(s) 819, 879, 915, 1335, 1428
TseFI GTSAC 6 cut(s) 34, 307, 337, 545, 886, 1496
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 6 cut(s) 34, 307, 337, 545, 886, 1496
TspDTI ATGAA 5 cut(s) 286, 542, 980, 1263, 1433
TspGWI ACGGA 2 cut(s) 548, 1295
TspMI CCCGGG 1 cut(s) 284
Vha464I CTTAAG 2 cut(s) 818, 914
VneI GTGCAC 1 cut(s) 1491
VpaK11BI GGWCC 1 cut(s) 324
XapI RAATTY 2 cut(s) 61, 487
XceI RCATGY 4 cut(s) 668, 1238, 1491, 1582
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1575
XmnI GAANNNNTTC 2 cut(s) 499, 1254
XspI CTAG 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.