Rh2DG442100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007866
Physical Location & Seq
Reverse (-)
64241472 .. 64249227
7756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG442100.1

Sequence Viewer

Length: 1059 bp
ATGGAGTTCTCATTTGGTTCTCAAAAATGGTTACCTAAAGAATCATTACACGAGAGACAAGATGGAATTGAGAATTATGCCTTGGGTCTGCATGCTCCTGGCTTTTTTGATAAGGTTCTAAATGTTGACAAGTGCTTATTGCAAAGTGAGCCTGCCGACATGGTTCTTGCCGCCGTACAACAAAGTTGGAGAGATCCACAATTAGGTCTCTCTCCTTTTGATGTTTACTCTCATGCCGGATTTCTTAAGCATCTAATGCTAAGAACTGGAAGGAATGTGAAGACCGGTCTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTTACAAGCCAGAGCTACTTAAGCCTCTTCTTCAAAGGATAACAGCTATCCCCGAAGTGGTAAGCGTCATGAACAATGTAAATACGTCTGTTGGTAACACGTCTCTTGGGGAGGTCGAGTACACTTTGTATGGAAAGTCTAGCATAACAGAGACCTTACGAGGGCTCACCTTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAGATTTTATATAAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGAGGGCTCAGAGATTGTTCTTGACCTGTTTTGTGGGACTGGCACCATTGGTCTGACACTGGCCAGAAGGGCCAAACATGTTTATGGCTATGAAATAATTCCTCAGGCCATTGCAGATGCTTGTCTGAATGCTGAGCTGAATGGTATCCGTAATGCTACATTTGTCCAAGGGGATCTTAACAAAATTGATGAGAGTTTTGGGAACAATTTTCCTAAGCCTGACATCGTTATTTCAGATCCCAATCGTCCAGGAATGCACATGAAGTTGATTAAGTTTCTTCTAAAGCTGAAGGCTTCACGCATTGTTTATGTATCATGTAACCCTGCCACATGTGCACGTGACCTTGATTATCTCTGCAATGGTGTGGTAGAGAAAAACATAAAAGGCTGTTACAAGCTAAAGAGTGTGCAACCAGTAGACATGTTTCCGCACACTCCTCATATTGAGTGTGTTTGCCTCTTGGAGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

352

Amino Acids

39.12

Weight (kDa)

6.32

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 161 - 350 4.6e-16 tRNA (Uracil-5-)-methyltransferase
Methyltransf_15 PF09445 199 - 277 3.9e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 200 - 274 8e-13 Methyltransferase domain
Methyltransf_25 PF13649 201 - 259 1.2e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 626
AccI GTMKAC 1 cut(s) 1002
AciI CCGC 2 cut(s) 171, 1013
AclWI GGATC 3 cut(s) 188, 765, 815
AcoI YGGCCR 1 cut(s) 645
AcuI CTGAAG 1 cut(s) 893
AcvI CACGTG 1 cut(s) 923
AfaI GTAC 2 cut(s) 177, 443
AfiI CCNNNNNNNGG 3 cut(s) 203, 379, 483
AflII CTTAAG 2 cut(s) 245, 341
AflIII ACRYGT 4 cut(s) 420, 661, 914, 1005
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 2 cut(s) 356, 497
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 3 cut(s) 97, 534, 832
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 6 cut(s) 337, 368, 721, 871, 982, 1051
AluI AGCT 6 cut(s) 337, 368, 721, 871, 982, 1051
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 5 cut(s) 49, 212, 429, 467, 581
Alw44I GTGCAC 1 cut(s) 918
AlwI GGATC 3 cut(s) 188, 765, 815
AoxI GGCC 3 cut(s) 645, 654, 690
ApaLI GTGCAC 1 cut(s) 918
AsiGI ACCGGT 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 481, 524
AxyI CCTNAGG 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 922
BalI TGGCCA 1 cut(s) 647
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 489, 593
BauI CACGAG 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 923
BbsI GAAGAC 2 cut(s) 281, 287
Bbv12I GWGCWC 1 cut(s) 922
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 3 cut(s) 99, 536, 834
BciVI GTATCC 1 cut(s) 740
BcoDI GTCTC 5 cut(s) 49, 212, 429, 467, 581
BfaI CTAG 1 cut(s) 462
BfrI CTTAAG 2 cut(s) 245, 341
BfuI GTATCC 1 cut(s) 740
BglI GCCNNNNNGGC 1 cut(s) 653
BisI GCNGC 1 cut(s) 171
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 99, 536, 834
BmgBI CACGTC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 654
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 3 cut(s) 99, 536, 834
BmsI GCATC 2 cut(s) 259, 691
BpiI GAAGAC 2 cut(s) 281, 287
Bpu10I CCTNAGC 1 cut(s) 798
Bpu1102I GCTNAGC 1 cut(s) 717
BsaAI YACGTR 1 cut(s) 923
BsaBI GATNNNNATC 1 cut(s) 825
BsaI GGTCTC 3 cut(s) 212, 467, 581
BsaJI CCNNGG 2 cut(s) 81, 751
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 379, 483
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 4 cut(s) 271, 628, 648, 998
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 2 cut(s) 693, 949
Bse8I GATNNNNATC 1 cut(s) 825
BseBI CCWGG 3 cut(s) 99, 536, 834
BseDI CCNNGG 2 cut(s) 81, 751
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 3 cut(s) 203, 379, 483
BseMI GCAATG 2 cut(s) 693, 949
BseMII CTCAG 4 cut(s) 592, 606, 701, 708
BseNI ACTGG 4 cut(s) 271, 628, 648, 998
BseRI GAGGAG 2 cut(s) 597, 1011
BseSI GKGCMC 1 cut(s) 922
BshFI GGCC 3 cut(s) 647, 656, 692
BshNI GGYRCC 1 cut(s) 626
BshTI ACCGGT 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 922
BsiSI CCGG 2 cut(s) 237, 285
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 3 cut(s) 203, 379, 483
BsmAI GTCTC 5 cut(s) 49, 212, 429, 467, 581
BsmBI CGTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 634
BsmI GAATGC 2 cut(s) 718, 843
BsnI GGCC 3 cut(s) 647, 656, 692
Bso31I GGTCTC 3 cut(s) 212, 467, 581
Bsp1286I GDGCHC 3 cut(s) 489, 593, 922
Bsp143I GATC 3 cut(s) 193, 757, 820
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 2 cut(s) 171, 1013
BspANI GGCC 3 cut(s) 647, 656, 692
BspCNI CTCAG 4 cut(s) 591, 605, 700, 709
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 1 cut(s) 628
BspPI GGATC 3 cut(s) 188, 765, 815
BspT107I GGYRCC 1 cut(s) 626
BspTI CTTAAG 2 cut(s) 245, 341
BspTNI GGTCTC 3 cut(s) 212, 467, 581
BsrDI GCAATG 2 cut(s) 693, 949
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 4 cut(s) 271, 628, 648, 998
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 81, 751
BssMI GATC 3 cut(s) 193, 757, 820
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 81, 751
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 3 cut(s) 99, 536, 834
Bst6I CTCTTC 1 cut(s) 354
BstAFI CTTAAG 2 cut(s) 245, 341
BstAPI GCANNNNNTGC 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 923
BstC8I GCNNGC 2 cut(s) 93, 153
BstDEI CTNAG 6 cut(s) 260, 578, 592, 687, 717, 798
BstEII GGTNACC 1 cut(s) 30
BstKTI GATC 3 cut(s) 196, 760, 823
BstMAI GTCTC 5 cut(s) 49, 212, 429, 467, 581
BstMBI GATC 3 cut(s) 193, 757, 820
BstMWI GCNNNNNNNGC 5 cut(s) 148, 256, 343, 653, 917
BstNI CCWGG 3 cut(s) 99, 536, 834
BstNSI RCATGY 4 cut(s) 95, 665, 918, 1009
BstPI GGTNACC 1 cut(s) 30
BstSCI CCNGG 3 cut(s) 97, 534, 832
BstSLI GKGCMC 1 cut(s) 922
BstV2I GAAGAC 2 cut(s) 281, 287
BstX2I RGATCY 3 cut(s) 193, 757, 820
BstYI RGATCY 3 cut(s) 193, 757, 820
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 3 cut(s) 647, 656, 692
BtrI CACGTC 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 641
Cac8I GCNNGC 2 cut(s) 93, 153
CciI TCATGA 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 1 cut(s) 654
CseI GACGC 1 cut(s) 376
Csp6I GTAC 2 cut(s) 176, 442
CspAI ACCGGT 1 cut(s) 284
CviAII CATG 9 cut(s) 92, 160, 233, 391, 662, 844, 900, 915, 1006
CviQI GTAC 2 cut(s) 176, 442
DdeI CTNAG 6 cut(s) 260, 578, 592, 687, 717, 798
DpnI GATC 3 cut(s) 195, 759, 822
DpnII GATC 3 cut(s) 193, 757, 820
EaeI YGGCCR 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 81, 751
Eco24I GRGCYC 2 cut(s) 489, 593
Eco31I GGTCTC 3 cut(s) 212, 467, 581
Eco57I CTGAAG 1 cut(s) 893
Eco72I CACGTG 1 cut(s) 923
Eco81I CCTNAGG 1 cut(s) 687
Eco91I GGTNACC 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 30
EcoRII CCWGG 3 cut(s) 97, 534, 832
EcoT14I CCWWGG 2 cut(s) 81, 751
EcoT38I GRGCYC 2 cut(s) 489, 593
ErhI CCWWGG 2 cut(s) 81, 751
Esp3I CGTCTC 1 cut(s) 429
FaeI CATG 9 cut(s) 95, 163, 236, 394, 665, 847, 903, 918, 1009
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 1 cut(s) 634
FatI CATG 9 cut(s) 91, 159, 232, 390, 661, 843, 899, 914, 1005
FblI GTMKAC 1 cut(s) 1002
Fnu4HI GCNGC 1 cut(s) 171
FriOI GRGCYC 2 cut(s) 489, 593
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 1 cut(s) 462
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 3 cut(s) 647, 656, 692
HapII CCGG 2 cut(s) 237, 285
HgaI GACGC 1 cut(s) 376
Hin1II CATG 9 cut(s) 95, 163, 236, 394, 665, 847, 903, 918, 1009
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 1 cut(s) 41
HpaII CCGG 2 cut(s) 237, 285
HphI GGTGA 2 cut(s) 481, 524
Hpy166II GTNNAC 5 cut(s) 127, 226, 444, 920, 1003
Hpy188I TCNGA 5 cut(s) 581, 595, 639, 711, 820
Hpy188III TCNNGA 3 cut(s) 293, 391, 605
Hpy8I GTNNAC 5 cut(s) 127, 226, 444, 920, 1003
HpyAV CCTTC 3 cut(s) 264, 645, 868
HpyCH4IV ACGT 3 cut(s) 407, 422, 922
HpyCH4V TGCA 7 cut(s) 91, 142, 698, 841, 920, 942, 994
HpyF10VI GCNNNNNNNGC 5 cut(s) 148, 256, 343, 653, 917
HpyF3I CTNAG 6 cut(s) 260, 578, 592, 687, 717, 798
HpySE526I ACGT 3 cut(s) 407, 422, 922
Hsp92II CATG 9 cut(s) 95, 163, 236, 394, 665, 847, 903, 918, 1009
Kzo9I GATC 3 cut(s) 193, 757, 820
LmnI GCTCC 2 cut(s) 100, 1048
LweI GCATC 2 cut(s) 259, 691
MaeI CTAG 1 cut(s) 462
MaeII ACGT 3 cut(s) 407, 422, 922
MaeIII GTNAC 6 cut(s) 30, 313, 416, 902, 923, 974
MalI GATC 3 cut(s) 195, 759, 822
MboI GATC 3 cut(s) 193, 757, 820
MboII GAAGA 7 cut(s) 281, 292, 341, 344, 508, 575, 854
MflI RGATCY 3 cut(s) 193, 757, 820
MhlI GDGCHC 3 cut(s) 489, 593, 922
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 7 cut(s) 66, 73, 200, 307, 681, 768, 790
MluNI TGGCCA 1 cut(s) 647
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 9 cut(s) 328, 357, 427, 476, 575, 580, 696, 1032, 1052
Mox20I TGGCCA 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 647
MseI TTAA 5 cut(s) 246, 306, 342, 762, 855
MslI CAYNNNNRTG 1 cut(s) 666
Msp20I TGGCCA 1 cut(s) 647
MspCI CTTAAG 2 cut(s) 245, 341
MspI CCGG 2 cut(s) 237, 285
MspR9I CCNGG 3 cut(s) 99, 536, 834
Mva1269I GAATGC 2 cut(s) 718, 843
MvaI CCWGG 3 cut(s) 99, 536, 834
MwoI GCNNNNNNNGC 5 cut(s) 148, 256, 343, 653, 917
NdeII GATC 3 cut(s) 193, 757, 820
NlaIII CATG 9 cut(s) 95, 163, 236, 394, 665, 847, 903, 918, 1009
NlaIV GGNNCC 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 313, 923
NspI RCATGY 4 cut(s) 95, 665, 918, 1009
PaeI GCATGC 1 cut(s) 95
PagI TCATGA 1 cut(s) 390
PciI ACATGT 3 cut(s) 661, 914, 1005
PctI GAATGC 2 cut(s) 718, 843
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 1 cut(s) 172
PmaCI CACGTG 1 cut(s) 923
PmlI CACGTG 1 cut(s) 923
Ppu21I YACGTR 1 cut(s) 923
PscI ACATGT 3 cut(s) 661, 914, 1005
Psp6I CCWGG 3 cut(s) 97, 534, 832
PspCI CACGTG 1 cut(s) 923
PspEI GGTNACC 1 cut(s) 30
PspGI CCWGG 3 cut(s) 97, 534, 832
PspN4I GGNNCC 1 cut(s) 628
PspPI GGNCC 1 cut(s) 654
PsuI RGATCY 3 cut(s) 193, 757, 820
RsaI GTAC 2 cut(s) 177, 443
RsaNI GTAC 2 cut(s) 176, 442
RseI CAYNNNNRTG 1 cut(s) 666
SaqAI TTAA 5 cut(s) 246, 306, 342, 762, 855
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 3 cut(s) 193, 757, 820
Sau96I GGNCC 1 cut(s) 654
ScrFI CCNGG 3 cut(s) 99, 536, 834
SduI GDGCHC 3 cut(s) 489, 593, 922
SfaNI GCATC 2 cut(s) 259, 691
SfiI GGCCNNNNNGGCC 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 245, 341
SmoI CTYRAG 2 cut(s) 245, 341
SphI GCATGC 1 cut(s) 95
Sse9I AATT 7 cut(s) 66, 73, 200, 307, 681, 768, 790
SsiI CCGC 2 cut(s) 171, 1013
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 3 cut(s) 97, 534, 832
StyI CCWWGG 2 cut(s) 81, 751
TaiI ACGT 3 cut(s) 410, 425, 925
TaqI TCGA 1 cut(s) 438
TasI AATT 7 cut(s) 66, 73, 200, 307, 681, 768, 790
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 5 cut(s) 246, 306, 342, 762, 855
Tru9I TTAA 5 cut(s) 246, 306, 342, 762, 855
TscAI CASTG 1 cut(s) 648
TseFI GTSAC 2 cut(s) 313, 923
Tsp45I GTSAC 2 cut(s) 313, 923
TspDTI ATGAA 3 cut(s) 407, 690, 860
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 1 cut(s) 648
Vha464I CTTAAG 2 cut(s) 245, 341
VneI GTGCAC 1 cut(s) 918
XceI RCATGY 4 cut(s) 95, 665, 918, 1009
XmiI GTMKAC 1 cut(s) 1002
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.