Rroxscaffold_2G00103680
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007866
Physical Location & Seq
Forward (+)
26655125 .. 26663219
8095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103680.1

Sequence Viewer

Length: 1683 bp
ATGGCGACGATGCCGAGCCACGGCCTGAGGCACGTGACCACGGTTCGCCCGTGGCTGCGGAATTTCCGAAGCGCGACGACGCTGGCGGCTCTGCCGTCTTCTCCTTCGGCCGAAGATTCGGAGCGGGCCGAGTCCAAGGAGAAGCCCAAGCCCAAGCCCAAGCCCAAGGCCAGCTCGTATTTCCCGAAGCGAGGGCAGAGTTTGGAGCTGGTGTGTGAGTCTTTGGCTTTCAAAGGGAAGGGTCTGTGTAAGGTTTCGGAGACCGGGTTCGTCGTCATGTGCGACCGGGCTTTGCCTGGGGAGCGGTTCATTGGGCGCGTGACTCGCAAGAAAGGGACCTATGCTGAGGTGACAAAGGTACAGACACTATCTCCGCATTGGGATTTCGTGGATGCTCCTTGTGAGTATGCATCTTACTGTGGAGGATGTAAAACACAAAATTTGTCCTATGAGGCGCAAATCAGAGCTAAGGAATACCAAGTTCGTGAGTTGATAATAAATTTTGGAAAGGTTTCTCCTACAGAGCTGGAAAGCATCATGAAGCCCATTGTTCCCTGTGACATCCAGTTCCGTTACAGGAATAAGGCTAGACCACAGACAATAATGGAAAATAATGACAACGAGATGGAATTCTCATTTGGTTCTCAAAAATGGTTACCTAAAGAATCATTACATGAGAGACAAGATGGAATTGAGAATTATGCCTTGGGTCTGCATGCTCCTGGCTTTTTTGATAAGGTTCTAAATGTTGACAAGTGCTTATTGCAAAGTGAGCCTGCCGATATGGTTCTTGCCGCCGTGCAACAAAGTTGGAGAGATCCACAATTAGGTCTCTCTCCTTATGATGTTTACTCTCATGCCGGATTTCTTAAGCATCTAATGCTAAGAACTGGAAGGAATGTGAAGACCGGTTTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTTACAAGCCAGAGCTCCTTAAGCCTCTTCTTCAAAGGATAACAGCTATCCCCGAAGTGGTAAGTGTCATGAACAATGTAAATACGTCTGTTGGTAACACGTCTCTTGGGGAGGTCGAGTACACTTTGTATGGAAAGTGTAGCATAACAGAGACCTTACGAGGGCTCACCTTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAGATTTTATATAAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGAGGGCTCAGAGATTGTTCTTGACCTGTTTTGTGGGACTGGCACCATTGGTCTGACACTTGCCAGAAGGGCCAAACATGTTTATGGCTATGAAATAATTCCTCAGGCCATTGCAGATGCTTGTCTGAATGCTGAGCTGAATGGTATCCGTAACGCCACATTTGTCCAAGGGGATCTTAACAAAATTGATGAAAGTTTTGGGAACAATTTTCCTAAGCCTGACATCGTTATTTCAGATCCCAACCGTCCAGGAATGCACATGAAGTTGATTAAGTTTCTTCTAAAGCTGAAGGCTTCACGCATTGTTTATGTATCATGTAACCCTGCCACATGTGCGCGTGACCTTGATTATCTCTGCAATGGTGTGGTAGAGAAAAACATAAAAGGCTGTTACAAGCTAAAGAGTGTGCAACCAGTAGACATGTTTCCTCACACTCCTCATATTGAGTGTGTTTGCCTCTTGGAGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

560

Amino Acids

62.61

Weight (kDa)

8.48

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 369 - 558 1.6e-15 tRNA (Uracil-5-)-methyltransferase
Methyltransf_15 PF09445 407 - 485 8.4e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 408 - 482 1.8e-12 Methyltransferase domain
Methyltransf_25 PF13649 409 - 467 2.3e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1250
AccBSI CCGCTC 2 cut(s) 124, 304
AccI GTMKAC 1 cut(s) 1626
AccII CGCG 3 cut(s) 74, 318, 1546
AciI CCGC 6 cut(s) 58, 86, 124, 304, 374, 795
AclWI GGATC 3 cut(s) 812, 1389, 1439
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 4 cut(s) 61, 439, 499, 629
AcuI CTGAAG 1 cut(s) 1517
AcvI CACGTG 1 cut(s) 34
AfaI GTAC 2 cut(s) 360, 1067
AfiI CCNNNNNNNGG 5 cut(s) 20, 191, 827, 1003, 1107
AflII CTTAAG 2 cut(s) 869, 965
AflIII ACRYGT 4 cut(s) 1044, 1285, 1538, 1629
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 3 cut(s) 232, 980, 1121
AjiI CACGTC 1 cut(s) 1047
AjnI CCWGG 4 cut(s) 295, 721, 1158, 1456
AjuI GAANNNNNNNTTGG 4 cut(s) 621, 653, 689, 721
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 6 cut(s) 254, 673, 836, 1053, 1091, 1205
AlwI GGATC 3 cut(s) 812, 1389, 1439
AoxI GGCC 6 cut(s) 22, 108, 126, 168, 1278, 1314
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 4 cut(s) 61, 439, 499, 629
AsiGI ACCGGT 1 cut(s) 908
Asp700I GAANNNNTTC 2 cut(s) 511, 1305
AspLEI GCGC 4 cut(s) 74, 318, 457, 1546
AspS9I GGNCC 3 cut(s) 126, 336, 1278
AsuC2I CCSGG 2 cut(s) 265, 287
AsuHPI GGTGA 3 cut(s) 361, 1105, 1148
AvaII GGWCC 1 cut(s) 336
AxyI CCTNAGG 2 cut(s) 26, 1311
BanI GGYRCC 1 cut(s) 1250
BanII GRGCYC 3 cut(s) 963, 1113, 1217
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 2 cut(s) 90, 911
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 42
BccI CCATC 2 cut(s) 619, 680
BceAI ACGGC 3 cut(s) 37, 79, 782
BciT130I CCWGG 4 cut(s) 297, 723, 1160, 1458
BciVI GTATCC 1 cut(s) 1364
BcnI CCSGG 2 cut(s) 265, 287
BcoDI GTCTC 6 cut(s) 254, 673, 836, 1053, 1091, 1205
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 519
BfrI CTTAAG 2 cut(s) 869, 965
BfuI GTATCC 1 cut(s) 1364
BglI GCCNNNNNGGC 1 cut(s) 1277
BisI GCNGC 3 cut(s) 56, 87, 795
BlpI GCTNAGC 1 cut(s) 1341
BlsI GCNGC 3 cut(s) 57, 88, 796
Bme1390I CCNGG 6 cut(s) 265, 287, 297, 723, 1160, 1458
Bme18I GGWCC 1 cut(s) 336
BmgBI CACGTC 1 cut(s) 1047
BmgT120I GGNCC 3 cut(s) 126, 336, 1278
BmiI GGNNCC 2 cut(s) 337, 1252
BmrFI CCNGG 6 cut(s) 265, 287, 297, 723, 1160, 1458
BmsI GCATC 5 cut(s) 382, 419, 543, 883, 1315
BpiI GAAGAC 2 cut(s) 90, 911
Bpu10I CCTNAGC 3 cut(s) 345, 468, 1422
Bpu1102I GCTNAGC 1 cut(s) 1341
BpuMI CCSGG 2 cut(s) 265, 287
BsaAI YACGTR 1 cut(s) 34
BsaI GGTCTC 4 cut(s) 254, 836, 1091, 1205
BsaJI CCNNGG 8 cut(s) 19, 39, 50, 135, 165, 296, 705, 1375
BsaWI WCCGGW 1 cut(s) 908
BsaXI ACNNNNNCTCC 8 cut(s) 197, 227, 251, 281, 355, 385, 1049, 1079
Bsc4I CCNNNNNNNGG 5 cut(s) 20, 191, 827, 1003, 1107
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 4 cut(s) 565, 895, 1252, 1622
Bse21I CCTNAGG 2 cut(s) 26, 1311
Bse3DI GCAATG 2 cut(s) 1317, 1573
BseBI CCWGG 4 cut(s) 297, 723, 1160, 1458
BseDI CCNNGG 8 cut(s) 19, 39, 50, 135, 165, 296, 705, 1375
BseGI GGATG 3 cut(s) 397, 431, 561
BseLI CCNNNNNNNGG 5 cut(s) 20, 191, 827, 1003, 1107
BseMI GCAATG 2 cut(s) 1317, 1573
BseMII CTCAG 6 cut(s) 17, 336, 1216, 1230, 1325, 1332
BseNI ACTGG 4 cut(s) 565, 895, 1252, 1622
BseRI GAGGAG 2 cut(s) 1221, 1635
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 42
Bsh1236I CGCG 3 cut(s) 74, 318, 1546
Bsh1285I CGRYCG 2 cut(s) 111, 286
BshFI GGCC 6 cut(s) 24, 110, 128, 170, 1280, 1316
BshNI GGYRCC 1 cut(s) 1250
BshTI ACCGGT 1 cut(s) 908
BsiEI CGRYCG 2 cut(s) 111, 286
BsiHKAI GWGCWC 1 cut(s) 963
BsiSI CCGG 4 cut(s) 264, 286, 861, 909
BslFI GGGAC 2 cut(s) 349, 1258
BslI CCNNNNNNNGG 5 cut(s) 20, 191, 827, 1003, 1107
BsmAI GTCTC 6 cut(s) 254, 673, 836, 1053, 1091, 1205
BsmBI CGTCTC 1 cut(s) 1053
BsmFI GGGAC 2 cut(s) 349, 1258
BsmI GAATGC 2 cut(s) 1342, 1467
BsnI GGCC 6 cut(s) 24, 110, 128, 170, 1280, 1316
Bso31I GGTCTC 4 cut(s) 254, 836, 1091, 1205
Bsp1286I GDGCHC 3 cut(s) 963, 1113, 1217
Bsp143I GATC 3 cut(s) 817, 1381, 1444
Bsp1720I GCTNAGC 1 cut(s) 1341
BspACI CCGC 6 cut(s) 58, 86, 124, 304, 374, 795
BspANI GGCC 6 cut(s) 24, 110, 128, 170, 1280, 1316
BspCNI CTCAG 6 cut(s) 18, 337, 1215, 1229, 1324, 1333
BspFNI CGCG 3 cut(s) 74, 318, 1546
BspHI TCATGA 2 cut(s) 537, 1014
BspLI GGNNCC 2 cut(s) 337, 1252
BspPI GGATC 3 cut(s) 812, 1389, 1439
BspT107I GGYRCC 1 cut(s) 1250
BspTI CTTAAG 2 cut(s) 869, 965
BspTNI GGTCTC 4 cut(s) 254, 836, 1091, 1205
BsrBI CCGCTC 2 cut(s) 124, 304
BsrDI GCAATG 2 cut(s) 1317, 1573
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 4 cut(s) 565, 895, 1252, 1622
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 8 cut(s) 19, 39, 50, 135, 165, 296, 705, 1375
BssMI GATC 3 cut(s) 817, 1381, 1444
BssT1I CCWWGG 4 cut(s) 135, 165, 705, 1375
Bst2UI CCWGG 4 cut(s) 297, 723, 1160, 1458
Bst4CI ACNGT 3 cut(s) 43, 419, 1454
Bst6I CTCTTC 1 cut(s) 978
BstAFI CTTAAG 2 cut(s) 869, 965
BstAPI GCANNNNNTGC 1 cut(s) 880
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 5 cut(s) 84, 126, 172, 717, 777
BstDEI CTNAG 9 cut(s) 26, 345, 468, 884, 1202, 1216, 1311, 1341, 1422
BstDSI CCRYGG 3 cut(s) 19, 39, 50
BstEII GGTNACC 1 cut(s) 654
BstF5I GGATG 3 cut(s) 397, 431, 561
BstFNI CGCG 3 cut(s) 74, 318, 1546
BstHHI GCGC 4 cut(s) 74, 318, 457, 1546
BstKTI GATC 3 cut(s) 820, 1384, 1447
BstMAI GTCTC 6 cut(s) 254, 673, 836, 1053, 1091, 1205
BstMBI GATC 3 cut(s) 817, 1381, 1444
BstMCI CGRYCG 2 cut(s) 111, 286
BstMWI GCNNNNNNNGC 7 cut(s) 301, 324, 772, 880, 967, 1277, 1541
BstNI CCWGG 4 cut(s) 297, 723, 1160, 1458
BstNSI RCATGY 4 cut(s) 719, 1289, 1542, 1633
BstPI GGTNACC 1 cut(s) 654
BstSCI CCNGG 6 cut(s) 263, 285, 295, 721, 1158, 1456
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 3 cut(s) 74, 318, 1546
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 2 cut(s) 90, 911
BstX2I RGATCY 3 cut(s) 817, 1381, 1444
BstYI RGATCY 3 cut(s) 817, 1381, 1444
BstZI CGGCCG 1 cut(s) 108
Bsu36I CCTNAGG 2 cut(s) 26, 1311
BsuI GTATCC 1 cut(s) 1364
BsuRI GGCC 6 cut(s) 24, 110, 128, 170, 1280, 1316
BtgI CCRYGG 3 cut(s) 19, 39, 50
BtrI CACGTC 1 cut(s) 1047
BtsCI GGATG 3 cut(s) 397, 431, 561
Cac8I GCNNGC 5 cut(s) 84, 126, 172, 717, 777
CciI TCATGA 2 cut(s) 537, 1014
CfoI GCGC 4 cut(s) 74, 318, 457, 1546
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 3 cut(s) 126, 336, 1278
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 359, 1066
CspAI ACCGGT 1 cut(s) 908
CviQI GTAC 2 cut(s) 359, 1066
DdeI CTNAG 9 cut(s) 26, 345, 468, 884, 1202, 1216, 1311, 1341, 1422
DpnI GATC 3 cut(s) 819, 1383, 1446
DpnII GATC 3 cut(s) 817, 1381, 1444
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Ecl136II GAGCTC 1 cut(s) 961
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 4 cut(s) 135, 165, 705, 1375
Eco24I GRGCYC 3 cut(s) 963, 1113, 1217
Eco31I GGTCTC 4 cut(s) 254, 836, 1091, 1205
Eco47I GGWCC 1 cut(s) 336
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 1517
Eco72I CACGTG 1 cut(s) 34
Eco81I CCTNAGG 2 cut(s) 26, 1311
Eco91I GGTNACC 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 961
EcoO109I RGGNCCY 1 cut(s) 336
EcoO65I GGTNACC 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 4 cut(s) 295, 721, 1158, 1456
EcoT14I CCWWGG 4 cut(s) 135, 165, 705, 1375
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 3 cut(s) 963, 1113, 1217
ErhI CCWWGG 4 cut(s) 135, 165, 705, 1375
Esp3I CGTCTC 1 cut(s) 1053
FalI AAGNNNNNCTT 2 cut(s) 1368, 1400
FaqI GGGAC 2 cut(s) 349, 1258
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 1626
Fnu4HI GCNGC 3 cut(s) 56, 87, 795
FokI GGATG 3 cut(s) 404, 438, 548
FriOI GRGCYC 3 cut(s) 963, 1113, 1217
Fsp4HI GCNGC 3 cut(s) 56, 87, 795
FspBI CTAG 1 cut(s) 588
GlaI GCGC 4 cut(s) 73, 317, 456, 1545
GluI GCNGC 3 cut(s) 56, 87, 795
HaeIII GGCC 6 cut(s) 24, 110, 128, 170, 1280, 1316
HapII CCGG 4 cut(s) 264, 286, 861, 909
HgaI GACGC 1 cut(s) 88
HhaI GCGC 4 cut(s) 74, 318, 457, 1546
Hin6I GCGC 4 cut(s) 72, 316, 455, 1544
HinP1I GCGC 4 cut(s) 72, 316, 455, 1544
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 5 cut(s) 116, 131, 218, 322, 665
HpaII CCGG 4 cut(s) 264, 286, 861, 909
HphI GGTGA 3 cut(s) 361, 1105, 1148
Hpy166II GTNNAC 4 cut(s) 751, 850, 1068, 1627
Hpy188I TCNGA 9 cut(s) 68, 121, 259, 464, 1205, 1219, 1263, 1335, 1444
Hpy188III TCNNGA 6 cut(s) 184, 485, 538, 917, 1015, 1229
Hpy8I GTNNAC 4 cut(s) 751, 850, 1068, 1627
Hpy99I CGWCG 4 cut(s) 10, 79, 82, 275
HpyAV CCTTC 5 cut(s) 114, 232, 888, 1269, 1492
HpyCH4III ACNGT 3 cut(s) 43, 419, 1454
HpyCH4IV ACGT 3 cut(s) 33, 1031, 1046
HpyCH4V TGCA 8 cut(s) 410, 715, 766, 802, 1322, 1465, 1566, 1618
HpyF10VI GCNNNNNNNGC 7 cut(s) 301, 324, 772, 880, 967, 1277, 1541
HpyF3I CTNAG 9 cut(s) 26, 345, 468, 884, 1202, 1216, 1311, 1341, 1422
HpySE526I ACGT 3 cut(s) 33, 1031, 1046
HspAI GCGC 4 cut(s) 72, 316, 455, 1544
Kzo9I GATC 3 cut(s) 817, 1381, 1444
LmnI GCTCC 7 cut(s) 121, 205, 301, 400, 724, 966, 1672
Lsp1109I GCAGC 1 cut(s) 42
LweI GCATC 5 cut(s) 382, 419, 543, 883, 1315
MaeI CTAG 1 cut(s) 588
MaeII ACGT 3 cut(s) 33, 1031, 1046
MalI GATC 3 cut(s) 819, 1383, 1446
MbiI CCGCTC 2 cut(s) 124, 304
MboI GATC 3 cut(s) 817, 1381, 1444
MboII GAAGA 8 cut(s) 90, 125, 916, 965, 968, 1132, 1199, 1478
MflI RGATCY 3 cut(s) 817, 1381, 1444
MhlI GDGCHC 3 cut(s) 963, 1113, 1217
MlyI GAGTC 3 cut(s) 140, 227, 316
MmeI TCCRAC 1 cut(s) 791
Mph1103I ATGCAT 1 cut(s) 412
MroXI GAANNNNTTC 2 cut(s) 511, 1305
MseI TTAA 5 cut(s) 870, 930, 966, 1386, 1479
MslI CAYNNNNRTG 1 cut(s) 1290
MspCI CTTAAG 2 cut(s) 869, 965
MspI CCGG 4 cut(s) 264, 286, 861, 909
MspR9I CCNGG 6 cut(s) 265, 287, 297, 723, 1160, 1458
Mva1269I GAATGC 2 cut(s) 1342, 1467
MvaI CCWGG 4 cut(s) 297, 723, 1160, 1458
MvnI CGCG 3 cut(s) 74, 318, 1546
MwoI GCNNNNNNNGC 7 cut(s) 301, 324, 772, 880, 967, 1277, 1541
NciI CCSGG 2 cut(s) 265, 287
NdeII GATC 3 cut(s) 817, 1381, 1444
NlaIV GGNNCC 2 cut(s) 337, 1252
NmeAIII GCCGAG 2 cut(s) 39, 154
NmuCI GTSAC 6 cut(s) 34, 319, 349, 557, 937, 1547
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 4 cut(s) 719, 1289, 1542, 1633
PaeI GCATGC 1 cut(s) 719
PagI TCATGA 2 cut(s) 537, 1014
PciI ACATGT 3 cut(s) 1285, 1538, 1629
PcsI WCGNNNNNNNCGW 3 cut(s) 47, 182, 279
PctI GAATGC 2 cut(s) 1342, 1467
PdmI GAANNNNTTC 2 cut(s) 511, 1305
PfeI GAWTC 2 cut(s) 116, 665
PfoI TCCNGGA 1 cut(s) 1456
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 3 cut(s) 57, 88, 796
PleI GAGTC 3 cut(s) 139, 226, 316
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 3 cut(s) 139, 226, 316
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 336
PscI ACATGT 3 cut(s) 1285, 1538, 1629
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 336
Psp6I CCWGG 4 cut(s) 295, 721, 1158, 1456
PspCI CACGTG 1 cut(s) 34
PspEI GGTNACC 1 cut(s) 654
PspGI CCWGG 4 cut(s) 295, 721, 1158, 1456
PspN4I GGNNCC 2 cut(s) 337, 1252
PspPI GGNCC 3 cut(s) 126, 336, 1278
PspPPI RGGWCCY 1 cut(s) 336
PsuI RGATCY 3 cut(s) 817, 1381, 1444
RsaI GTAC 2 cut(s) 360, 1067
RsaNI GTAC 2 cut(s) 359, 1066
RseI CAYNNNNRTG 1 cut(s) 1290
SacI GAGCTC 1 cut(s) 963
SaqAI TTAA 5 cut(s) 870, 930, 966, 1386, 1479
SatI GCNGC 3 cut(s) 56, 87, 795
Sau3AI GATC 3 cut(s) 817, 1381, 1444
Sau96I GGNCC 3 cut(s) 126, 336, 1278
SchI GAGTC 3 cut(s) 140, 227, 316
ScrFI CCNGG 6 cut(s) 265, 287, 297, 723, 1160, 1458
SduI GDGCHC 3 cut(s) 963, 1113, 1217
SfaNI GCATC 5 cut(s) 382, 419, 543, 883, 1315
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 1290
SmlI CTYRAG 2 cut(s) 869, 965
SmoI CTYRAG 2 cut(s) 869, 965
SphI GCATGC 1 cut(s) 719
SsiI CCGC 6 cut(s) 58, 86, 124, 304, 374, 795
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 963
StyD4I CCNGG 6 cut(s) 263, 285, 295, 721, 1158, 1456
StyI CCWWGG 4 cut(s) 135, 165, 705, 1375
TaaI ACNGT 3 cut(s) 43, 419, 1454
TaiI ACGT 3 cut(s) 36, 1034, 1049
TaqI TCGA 1 cut(s) 1062
TatI WGTACW 1 cut(s) 1065
TauI GCSGC 2 cut(s) 89, 797
TfiI GAWTC 2 cut(s) 116, 665
Tru1I TTAA 5 cut(s) 870, 930, 966, 1386, 1479
Tru9I TTAA 5 cut(s) 870, 930, 966, 1386, 1479
TseFI GTSAC 6 cut(s) 34, 319, 349, 557, 937, 1547
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 6 cut(s) 34, 319, 349, 557, 937, 1547
TspDTI ATGAA 6 cut(s) 298, 554, 1031, 1314, 1413, 1484
TspGWI ACGGA 2 cut(s) 560, 1346
Vha464I CTTAAG 2 cut(s) 869, 965
VpaK11BI GGWCC 1 cut(s) 336
XapI RAATTY 4 cut(s) 61, 439, 499, 629
XceI RCATGY 4 cut(s) 719, 1289, 1542, 1633
XmiI GTMKAC 1 cut(s) 1626
XmnI GAANNNNTTC 2 cut(s) 511, 1305
XspI CTAG 1 cut(s) 588
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.