MD17G1179000.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007866
Physical Location & Seq
Forward (+)
20507057 .. 20517111
10055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1179000.v1.1.491

Sequence Viewer

Length: 1677 bp
ATGGCGAACGTGCCGAGCCACGGACTGAGGCACGTGACTGCCATTCGCCCGTGGCTCCCGACCTTCCGTCGCTCCACCTCCCTGACCATCATGCGCCCGTGGCTCCCCACCTTCCGTCGCTCCACCTCCCTGGCTTCTCTCTCCTCCTCTTACACCGAAGACTCGGAGCACGCCCAGCCCAAAAGCGGCGACAAGCCCAACTCGTACTTCCCGAAGCGAGGGCAGACTTTCGAGCTGGTGTGCGAGTCTCTGGCTTTCAAAGGGAAAGGTCTATGCAAGGTGGCAGAGACTGGGTTCGTCGTCATGTGCGACCGCGCGCTGCCCGGCGAGCGGTTCGTAGGGAGGGTTACTCGCCGGAAAGGGACCTATGCTGAGGTGACAAAGGTCGAGACAATTTCTCCTCCTTGGGACTTTGTGGACGCTCCTTGTGAGTATGCTTCTTACTGTGGTGGATGTAAAACACAAAATTTGATTTATGAGGCACAAGTCAAAGCTAAGGAGCAGCAAGTTCATGAGTTGATTATAAACTTTGGAAAGTTTTCTCACACAGAACTGGAATCTCTAAGCATCATGAAGCCCATCGTTCCCTGTGAAATCCAGTTTCATTACAGGAATAAGATGGAGTTCTCATTTGGTTCTCAAAAATGGTTACCCAAGGAAAAATTGATGGAGAGACAAGATGGAATTGAGAACTATGCCCTGGGATTGCATGCTCCTGGATTCTTCGATAAGGTTCTGAATGTTGACAAGTGCTTATTGCAAAGTGAGCCGGCGAATATGGTTCTTGCTGCAGTCCAAGACTGTTGGAGAGATCCACAATTAGGTCTCTCTCCTTATGACGTTTACTCTCATGTTGGATTTCTTAAGCATCTAATGCTAAGAACTGGAAGGAATGCGGAGACTGGTCTTCCTGAACTTATGGTTAATTTTGTGACCTCATCTTACAAGCCAGAGCTGCTTAAGCCTCTTCTTGAAAAGATATCAGCTATCCCTGAAGTGGTAAGTGTCATGAACAATATAAATAGTTCTGTTGGTAACACATCTCTTGGGGAGGTGGAATACAATTTGTATGGAAAATCCAGCATCACGGAGAACTTAAGAGGGCTAACGTTTCAAATATCAGCAAACTCATTCTTCCAAACAAATACTCACCAGGCAGAGGTTTTATATGAACTCATAGAAGATTGTGCTGGTCTCAGAGGAGATGGCTCAGAGATTATTCTTGACCTGTTTTGTGGAACTGGCACCATTGGGTTGACACTTGCCAGAAGGGCCAAACATGTTTATGGATATGAAATAATTCCTCAAGCCATTGCAGATGCTTGTCTAAATGCCGAGCTGAATGGCATCCAGAATGCCACATTTGTCCAAGGGGATCTTAACAAAATTGATGAAAGTTTTGGGAAAAATTTTCCTAAGCCCGACATAGTTATTTCAGATCCAAACCGTCCAGGAATGCACATGAAGTTGATAAGATATCTTCTAAAGCTTAAGGCGCCACGGATAGTTTATGTATCGTGTAATCCTGCCACATGTGCACGTGACCTTGATTATCTCTGTCATGGTGTGACTGAGAAAGGTATAGAAGGCTGTTATAAGCTAAAGAGCGTACAGCCAGTAGACATGTTCCCTCACACTCCTCATATCGAGTGTGTTTGCCTTTTGGAGCTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_U5-meth_tr

Protein Analysis

559

Amino Acids

62.53

Weight (kDa)

7.19

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 366 - 556 7.1e-16 tRNA (Uracil-5-)-methyltransferase
Methyltransf_15 PF09445 405 - 482 3.9e-06 RNA cap guanine-N2 methyltransferase
Methyltransf_31 PF13847 406 - 509 8.5e-13 Methyltransferase domain
Methyltransf_25 PF13649 407 - 465 3.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 524, 1596
AccB1I GGYRCC 2 cut(s) 1244, 1495
AccBSI CCGCTC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 1620
AccII CGCG 2 cut(s) 315, 317
AciI CCGC 4 cut(s) 186, 313, 331, 896
AclI AACGTT 1 cut(s) 1109
AclWI GGATC 3 cut(s) 806, 1383, 1433
AcsI RAATTY 2 cut(s) 466, 1408
AcuI CTGAAG 1 cut(s) 1014
AcvI CACGTG 2 cut(s) 34, 1541
AcyI GRCGYC 1 cut(s) 1496
AfaI GTAC 2 cut(s) 206, 1611
AfiI CCNNNNNNNGG 7 cut(s) 20, 185, 218, 330, 821, 997, 1159
AflII CTTAAG 4 cut(s) 863, 959, 1096, 1490
AflIII ACRYGT 3 cut(s) 1279, 1532, 1623
AgsI TTSAA 3 cut(s) 259, 974, 1115
AhdI GACNNNNNGTC 1 cut(s) 66
AjnI CCWGG 5 cut(s) 129, 699, 715, 1152, 1450
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 8 cut(s) 235, 494, 955, 986, 1339, 1489, 1600, 1669
AluI AGCT 8 cut(s) 235, 494, 955, 986, 1339, 1489, 1600, 1669
Alw21I GWGCWC 2 cut(s) 171, 1540
Alw26I GTCTC 7 cut(s) 252, 281, 383, 667, 830, 893, 1199
Alw44I GTGCAC 1 cut(s) 1536
AlwI GGATC 3 cut(s) 806, 1383, 1433
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 1 cut(s) 1272
ApaLI GTGCAC 1 cut(s) 1536
ApeKI GCWGC 4 cut(s) 319, 502, 788, 955
ApoI RAATTY 2 cut(s) 466, 1408
Asp700I GAANNNNTTC 2 cut(s) 538, 1299
AspLEI GCGC 4 cut(s) 96, 317, 319, 1498
AspS9I GGNCC 2 cut(s) 363, 1272
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 2 cut(s) 388, 1142
AvaII GGWCC 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 1540
BanI GGYRCC 2 cut(s) 1244, 1495
BbrPI CACGTG 2 cut(s) 34, 1541
BbsI GAAGAC 2 cut(s) 165, 899
Bbv12I GWGCWC 2 cut(s) 171, 1540
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 4 cut(s) 306, 514, 775, 942
BccI CCATC 6 cut(s) 95, 587, 613, 661, 674, 1199
BciT130I CCWGG 5 cut(s) 131, 701, 717, 1154, 1452
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 7 cut(s) 252, 281, 383, 667, 830, 893, 1199
BfmI CTRYAG 1 cut(s) 789
BfoI RGCGCY 1 cut(s) 1499
BfrI CTTAAG 4 cut(s) 863, 959, 1096, 1490
BglI GCCNNNNNGGC 1 cut(s) 1271
BisI GCNGC 5 cut(s) 187, 320, 503, 789, 956
BlsI GCNGC 5 cut(s) 188, 321, 504, 790, 957
Bme1390I CCNGG 6 cut(s) 131, 324, 701, 717, 1154, 1452
Bme18I GGWCC 1 cut(s) 363
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgT120I GGNCC 2 cut(s) 363, 1272
BmiI GGNNCC 5 cut(s) 56, 104, 364, 1246, 1497
BmrFI CCNGG 6 cut(s) 131, 324, 701, 717, 1154, 1452
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 5 cut(s) 576, 877, 1092, 1309, 1356
BmuI ACTGGG 1 cut(s) 300
BoxI GACNNNNGTC 1 cut(s) 383
BpiI GAAGAC 2 cut(s) 165, 899
BplI GAGNNNNNCTC 2 cut(s) 334, 366
Bpu10I CCTNAGC 3 cut(s) 372, 495, 1416
BpuEI CTTGAG 1 cut(s) 1290
BpuMI CCSGG 1 cut(s) 324
BsaAI YACGTR 2 cut(s) 34, 1541
BsaHI GRCGYC 1 cut(s) 1496
BsaI GGTCTC 2 cut(s) 830, 1199
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 7 cut(s) 20, 185, 218, 330, 821, 997, 1159
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 7 cut(s) 295, 558, 598, 889, 907, 1246, 1616
Bse3DI GCAATG 1 cut(s) 1311
BseBI CCWGG 5 cut(s) 131, 701, 717, 1154, 1452
BseGI GGATG 2 cut(s) 458, 1347
BseLI CCNNNNNNNGG 7 cut(s) 20, 185, 218, 330, 821, 997, 1159
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 5 cut(s) 17, 363, 1210, 1224, 1563
BseNI ACTGG 7 cut(s) 295, 558, 598, 889, 907, 1246, 1616
BsePI GCGCGC 1 cut(s) 315
BseRI GAGGAG 5 cut(s) 133, 136, 390, 1215, 1629
BseSI GKGCMC 1 cut(s) 1540
BseXI GCAGC 4 cut(s) 306, 514, 775, 942
BseYI CCCAGC 1 cut(s) 174
Bsh1236I CGCG 2 cut(s) 315, 317
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 1 cut(s) 1274
BshNI GGYRCC 2 cut(s) 1244, 1495
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 2 cut(s) 171, 1540
BsiSI CCGG 3 cut(s) 324, 355, 770
BslFI GGGAC 2 cut(s) 376, 422
BslI CCNNNNNNNGG 7 cut(s) 20, 185, 218, 330, 821, 997, 1159
BsmAI GTCTC 7 cut(s) 252, 281, 383, 667, 830, 893, 1199
BsmFI GGGAC 2 cut(s) 376, 422
BsmI GAATGC 3 cut(s) 898, 1360, 1461
BsnI GGCC 1 cut(s) 1274
Bso31I GGTCTC 2 cut(s) 830, 1199
Bsp1286I GDGCHC 2 cut(s) 171, 1540
Bsp143I GATC 3 cut(s) 811, 1375, 1438
BspACI CCGC 4 cut(s) 186, 313, 331, 896
BspANI GGCC 1 cut(s) 1274
BspCNI CTCAG 5 cut(s) 18, 364, 1209, 1223, 1564
BspFNI CGCG 2 cut(s) 315, 317
BspHI TCATGA 3 cut(s) 511, 570, 1008
BspLI GGNNCC 5 cut(s) 56, 104, 364, 1246, 1497
BspMAI CTGCAG 1 cut(s) 793
BspPI GGATC 3 cut(s) 806, 1383, 1433
BspT107I GGYRCC 2 cut(s) 1244, 1495
BspTI CTTAAG 4 cut(s) 863, 959, 1096, 1490
BspTNI GGTCTC 2 cut(s) 830, 1199
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 1311
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 7 cut(s) 295, 558, 598, 889, 907, 1246, 1616
BssAI RCCGGY 1 cut(s) 769
BssHII GCGCGC 1 cut(s) 315
BssMI GATC 3 cut(s) 811, 1375, 1438
BssNI GRCGYC 1 cut(s) 1496
BssT1I CCWWGG 3 cut(s) 404, 654, 1369
Bst2UI CCWGG 5 cut(s) 131, 701, 717, 1154, 1452
Bst4CI ACNGT 3 cut(s) 446, 803, 1448
Bst6I CTCTTC 1 cut(s) 972
BstACI GRCGYC 1 cut(s) 1496
BstAFI CTTAAG 4 cut(s) 863, 959, 1096, 1490
BstAPI GCANNNNNTGC 1 cut(s) 874
BstBAI YACGTR 2 cut(s) 34, 1541
BstC8I GCNNGC 5 cut(s) 171, 317, 329, 711, 771
BstDEI CTNAG 9 cut(s) 26, 372, 495, 563, 878, 1196, 1210, 1416, 1572
BstDSI CCRYGG 4 cut(s) 19, 50, 98, 1499
BstEII GGTNACC 1 cut(s) 648
BstF5I GGATG 2 cut(s) 458, 1347
BstFNI CGCG 2 cut(s) 315, 317
BstH2I RGCGCY 1 cut(s) 1499
BstHHI GCGC 4 cut(s) 96, 317, 319, 1498
BstKTI GATC 3 cut(s) 814, 1378, 1441
BstMAI GTCTC 7 cut(s) 252, 281, 383, 667, 830, 893, 1199
BstMBI GATC 3 cut(s) 811, 1375, 1438
BstMCI CGRYCG 1 cut(s) 313
BstNI CCWGG 5 cut(s) 131, 701, 717, 1154, 1452
BstNSI RCATGY 4 cut(s) 713, 1283, 1536, 1627
BstPAI GACNNNNGTC 1 cut(s) 383
BstPI GGTNACC 1 cut(s) 648
BstSCI CCNGG 6 cut(s) 129, 322, 699, 715, 1152, 1450
BstSFI CTRYAG 1 cut(s) 789
BstSLI GKGCMC 1 cut(s) 1540
BstUI CGCG 2 cut(s) 315, 317
BstV1I GCAGC 4 cut(s) 306, 514, 775, 942
BstV2I GAAGAC 2 cut(s) 165, 899
BstX2I RGATCY 3 cut(s) 811, 1375, 1438
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 3 cut(s) 811, 1375, 1438
BsuRI GGCC 1 cut(s) 1274
BtgI CCRYGG 4 cut(s) 19, 50, 98, 1499
BtsCI GGATG 2 cut(s) 458, 1347
Cac8I GCNNGC 5 cut(s) 171, 317, 329, 711, 771
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 3 cut(s) 511, 570, 1008
CfoI GCGC 4 cut(s) 96, 317, 319, 1498
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 2 cut(s) 363, 1272
CseI GACGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 205, 1610
CviQI GTAC 2 cut(s) 205, 1610
DdeI CTNAG 9 cut(s) 26, 372, 495, 563, 878, 1196, 1210, 1416, 1572
DinI GGCGCC 1 cut(s) 1497
DpnI GATC 3 cut(s) 813, 1377, 1440
DpnII GATC 3 cut(s) 811, 1375, 1438
DriI GACNNNNNGTC 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 972
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 972
Eco130I CCWWGG 3 cut(s) 404, 654, 1369
Eco31I GGTCTC 2 cut(s) 830, 1199
Eco32I GATATC 2 cut(s) 981, 1478
Eco47I GGWCC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 1014
Eco72I CACGTG 2 cut(s) 34, 1541
Eco91I GGTNACC 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 363
EcoO65I GGTNACC 1 cut(s) 648
EcoRII CCWGG 5 cut(s) 129, 699, 715, 1152, 1450
EcoRV GATATC 2 cut(s) 981, 1478
EcoT14I CCWWGG 3 cut(s) 404, 654, 1369
EgeI GGCGCC 1 cut(s) 1497
EheI GGCGCC 1 cut(s) 1497
ErhI CCWWGG 3 cut(s) 404, 654, 1369
FalI AAGNNNNNCTT 2 cut(s) 1362, 1394
FaqI GGGAC 2 cut(s) 376, 422
FblI GTMKAC 1 cut(s) 1620
Fnu4HI GCNGC 5 cut(s) 187, 320, 503, 789, 956
FokI GGATG 2 cut(s) 465, 1334
Fsp4HI GCNGC 5 cut(s) 187, 320, 503, 789, 956
GlaI GCGC 4 cut(s) 95, 316, 318, 1497
GluI GCNGC 5 cut(s) 187, 320, 503, 789, 956
GsaI CCCAGC 1 cut(s) 178
HaeII RGCGCY 1 cut(s) 1499
HaeIII GGCC 1 cut(s) 1274
HapII CCGG 3 cut(s) 324, 355, 770
HgaI GACGC 1 cut(s) 428
HhaI GCGC 4 cut(s) 96, 317, 319, 1498
Hin1I GRCGYC 1 cut(s) 1496
Hin6I GCGC 4 cut(s) 94, 315, 317, 1496
HinP1I GCGC 4 cut(s) 94, 315, 317, 1496
HincII GTYRAC 2 cut(s) 745, 1257
HindII GTYRAC 2 cut(s) 745, 1257
HindIII AAGCTT 1 cut(s) 1487
HinfI GANTC 4 cut(s) 161, 245, 557, 720
HpaII CCGG 3 cut(s) 324, 355, 770
HphI GGTGA 2 cut(s) 388, 1142
Hpy166II GTNNAC 6 cut(s) 418, 745, 844, 1257, 1538, 1621
Hpy188I TCNGA 5 cut(s) 166, 738, 1199, 1213, 1438
Hpy8I GTNNAC 6 cut(s) 418, 745, 844, 1257, 1538, 1621
Hpy99I CGWCG 3 cut(s) 72, 120, 302
HpyAV CCTTC 5 cut(s) 73, 121, 882, 1263, 1580
HpyCH4III ACNGT 3 cut(s) 446, 803, 1448
HpyCH4IV ACGT 5 cut(s) 9, 33, 840, 1109, 1540
HpyCH4V TGCA 7 cut(s) 276, 709, 760, 791, 1316, 1459, 1538
HpyF3I CTNAG 9 cut(s) 26, 372, 495, 563, 878, 1196, 1210, 1416, 1572
HpySE526I ACGT 5 cut(s) 9, 33, 840, 1109, 1540
Hsp92I GRCGYC 1 cut(s) 1496
HspAI GCGC 4 cut(s) 94, 315, 317, 1496
KasI GGCGCC 1 cut(s) 1495
KroI GCCGGC 1 cut(s) 769
KroNI GCCGGC 1 cut(s) 771
Kzo9I GATC 3 cut(s) 811, 1375, 1438
LmnI GCTCC 9 cut(s) 60, 77, 108, 125, 166, 427, 499, 718, 1666
Lsp1109I GCAGC 4 cut(s) 306, 514, 775, 942
LweI GCATC 5 cut(s) 576, 877, 1092, 1309, 1356
MaeII ACGT 5 cut(s) 9, 33, 840, 1109, 1540
MaeIII GTNAC 8 cut(s) 34, 346, 376, 648, 931, 1034, 1541, 1567
MalI GATC 3 cut(s) 813, 1377, 1440
MbiI CCGCTC 1 cut(s) 331
MboI GATC 3 cut(s) 811, 1375, 1438
MboII GAAGA 7 cut(s) 170, 715, 899, 959, 1126, 1193, 1472
MflI RGATCY 3 cut(s) 811, 1375, 1438
MhlI GDGCHC 2 cut(s) 171, 1540
Mly113I GGCGCC 1 cut(s) 1496
MlyI GAGTC 2 cut(s) 155, 254
MmeI TCCRAC 2 cut(s) 785, 835
MroNI GCCGGC 1 cut(s) 769
MroXI GAANNNNTTC 2 cut(s) 538, 1299
MseI TTAA 6 cut(s) 864, 924, 960, 1097, 1380, 1491
MslI CAYNNNNRTG 1 cut(s) 1284
MspCI CTTAAG 4 cut(s) 863, 959, 1096, 1490
MspI CCGG 3 cut(s) 324, 355, 770
MspR9I CCNGG 6 cut(s) 131, 324, 701, 717, 1154, 1452
Mva1269I GAATGC 3 cut(s) 898, 1360, 1461
MvaI CCWGG 5 cut(s) 131, 701, 717, 1154, 1452
MvnI CGCG 2 cut(s) 315, 317
NaeI GCCGGC 1 cut(s) 771
NarI GGCGCC 1 cut(s) 1496
NciI CCSGG 1 cut(s) 324
NdeII GATC 3 cut(s) 811, 1375, 1438
NgoMIV GCCGGC 1 cut(s) 769
NlaIV GGNNCC 5 cut(s) 56, 104, 364, 1246, 1497
NmeAIII GCCGAG 2 cut(s) 39, 1360
NmuCI GTSAC 5 cut(s) 34, 376, 931, 1541, 1567
NspI RCATGY 4 cut(s) 713, 1283, 1536, 1627
PaeI GCATGC 1 cut(s) 713
PagI TCATGA 3 cut(s) 511, 570, 1008
PasI CCCWGGG 1 cut(s) 700
PauI GCGCGC 1 cut(s) 315
PciI ACATGT 3 cut(s) 1279, 1532, 1623
PcsI WCGNNNNNNNCGW 2 cut(s) 209, 306
PctI GAATGC 3 cut(s) 898, 1360, 1461
PdiI GCCGGC 1 cut(s) 771
PdmI GAANNNNTTC 2 cut(s) 538, 1299
PfeI GAWTC 2 cut(s) 557, 720
PfoI TCCNGGA 2 cut(s) 715, 1450
PkrI GCNGC 5 cut(s) 188, 321, 504, 790, 957
PleI GAGTC 2 cut(s) 155, 253
PluTI GGCGCC 1 cut(s) 1499
PmaCI CACGTG 2 cut(s) 34, 1541
PmlI CACGTG 2 cut(s) 34, 1541
PpsI GAGTC 2 cut(s) 155, 253
Ppu21I YACGTR 2 cut(s) 34, 1541
PpuMI RGGWCCY 1 cut(s) 363
PscI ACATGT 3 cut(s) 1279, 1532, 1623
PshAI GACNNNNGTC 1 cut(s) 383
PsiI TTATAA 2 cut(s) 524, 1596
Psp1406I AACGTT 1 cut(s) 1109
Psp5II RGGWCCY 1 cut(s) 363
Psp6I CCWGG 5 cut(s) 129, 699, 715, 1152, 1450
PspCI CACGTG 2 cut(s) 34, 1541
PspEI GGTNACC 1 cut(s) 648
PspFI CCCAGC 1 cut(s) 174
PspGI CCWGG 5 cut(s) 129, 699, 715, 1152, 1450
PspN4I GGNNCC 5 cut(s) 56, 104, 364, 1246, 1497
PspPI GGNCC 2 cut(s) 363, 1272
PspPPI RGGWCCY 1 cut(s) 363
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 3 cut(s) 811, 1375, 1438
PteI GCGCGC 1 cut(s) 315
RsaI GTAC 2 cut(s) 206, 1611
RsaNI GTAC 2 cut(s) 205, 1610
RseI CAYNNNNRTG 1 cut(s) 1284
SaqAI TTAA 6 cut(s) 864, 924, 960, 1097, 1380, 1491
SatI GCNGC 5 cut(s) 187, 320, 503, 789, 956
Sau3AI GATC 3 cut(s) 811, 1375, 1438
Sau96I GGNCC 2 cut(s) 363, 1272
SchI GAGTC 2 cut(s) 155, 254
ScrFI CCNGG 6 cut(s) 131, 324, 701, 717, 1154, 1452
SduI GDGCHC 2 cut(s) 171, 1540
SfaNI GCATC 5 cut(s) 576, 877, 1092, 1309, 1356
SfcI CTRYAG 1 cut(s) 789
SfoI GGCGCC 1 cut(s) 1497
SinI GGWCC 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 1284
SmlI CTYRAG 5 cut(s) 863, 959, 1096, 1305, 1490
SmoI CTYRAG 5 cut(s) 863, 959, 1096, 1305, 1490
SphI GCATGC 1 cut(s) 713
SsiI CCGC 4 cut(s) 186, 313, 331, 896
SspDI GGCGCC 1 cut(s) 1495
StyD4I CCNGG 6 cut(s) 129, 322, 699, 715, 1152, 1450
StyI CCWWGG 3 cut(s) 404, 654, 1369
TaaI ACNGT 3 cut(s) 446, 803, 1448
TaiI ACGT 5 cut(s) 12, 36, 843, 1112, 1543
TaqI TCGA 4 cut(s) 231, 387, 726, 1647
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 2 cut(s) 557, 720
Tru1I TTAA 6 cut(s) 864, 924, 960, 1097, 1380, 1491
Tru9I TTAA 6 cut(s) 864, 924, 960, 1097, 1380, 1491
TseFI GTSAC 5 cut(s) 34, 376, 931, 1541, 1567
TseI GCWGC 4 cut(s) 319, 502, 788, 955
Tsp45I GTSAC 5 cut(s) 34, 376, 931, 1541, 1567
TspDTI ATGAA 8 cut(s) 500, 587, 593, 1025, 1185, 1308, 1407, 1478
TspGWI ACGGA 5 cut(s) 36, 56, 104, 1103, 1516
Vha464I CTTAAG 4 cut(s) 863, 959, 1096, 1490
VneI GTGCAC 1 cut(s) 1536
VpaK11BI GGWCC 1 cut(s) 363
XapI RAATTY 2 cut(s) 466, 1408
XceI RCATGY 4 cut(s) 713, 1283, 1536, 1627
XmiI GTMKAC 1 cut(s) 1620
XmnI GAANNNNTTC 2 cut(s) 538, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.