AT3G21420

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014234
Physical Location & Seq
Forward (+)
7541384 .. 7543560
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G21420.1

Sequence Viewer

Length: 1095 bp
ATGGCTCCTCTACCTATTTCTTCGATTAGAGTTGGGAAAATAGATGATGTTCAAGAACTCATAAAATCCAAACCAAACAAAGTTCCAGAGAGATTCATTAGAGAAGAGTATGAGAGAGGAGTCGTTGTGTCGTCTCTTAAGACTCATCATCTTCATCATCAGATTCCTGTTATTGATCTCTCTAAGCTCTCTAAACCCGACAATGATGATTTTTTCTTTGAGATTCTTAAGCTCTCACAAGCTTGTGAAGACTGGGGATTTTTCCAGGTTATAAATCATGGGATAGAAGTGGAGGTTGTGGAAGATATAGAGGAAGTGGCTTCAGAGTTCTTTGATATGCCATTGGAGGAGAAGAAGAAGTATCCAATGGAGCCAGGTACCGTACAAGGTTATGGTCAAGCTTTCATATTCTCCGAAGATCAGAAACTTGATTGGTGCAACATGTTTGCCCTTGGTGTTCATCCTCCACAAATTCGCAACCCCAAACTATGGCCCTCTAAACCGGCCCGGTTCAGTGAAAGTCTTGAAGGTTACTCAAAAGAGATAAGGGAACTATGCAAAAGGTTGTTGAAGTATATAGCAATAAGCTTGGGTCTAAAAGAAGAGAGATTTGAGGAAATGTTTGGGGAGGCAGTGCAAGCAGTGAGGATGAATTATTACCCTCCATGTTCAAGTCCTGATCTTGTCTTAGGACTTAGTCCACATTCTGATGGAAGTGCTCTCACTGTTCTTCAACAGAGCAAGAACAGTTGTGTTGGCCTTCAGATCCTCAAAGACAACACTTGGGTCCCTGTTAAACCTCTTCCCAATGCCCTTGTCATCAACATTGGTGATACCATTGAGGTGCTAAGTAATGGGAAATACAAGAGTGTAGAGCATAGGGCAGTAACAAACAGGGAAAAGGAGAGGCTTACAATAGTGACATTCTATGCACCAAACTATGAGGTTGAAATAGAACCAATGAGTGAGTTGGTTGATGATGAGACCAACCCATGCAAGTATAGAAGCTACAATCATGGTGACTATAGTTATCACTATGTCTCCAACAAGCTCCAAGGCAAGAAGTCCCTTGATTTTGCCAAGATTCTCAATTAA

Protein Analysis

364

Amino Acids

41.69

Weight (kDa)

5.5

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 55 - 166 2.7e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 213 - 312 5.5e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21420
fragaria_vesca FvH4_4g17720 FvH4_4g17720
malus_domestica MD13G1170400.v1.1 MD16G1172000.v1.1
prunus_persica Prupe.1G138800_v2.0.a1
pyrus_communis pycom13g14590
rosa_chinensis RchiOBHm_Chr4g0421691
rosa_laevigata RLG00000007658
rosa_multiflora Rmu_sc0004533.1_g000010
rosa_roxburghii Rroxscaffold_5G00363930
rosa_rugosa Rorug04G0174700 Rorug04G0174800 Rorug04G0174900
rosa_samantha Rh4CG248900 Rh4DG232800
rosa_wichuraiana Rw4G020000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 272
Acc65I GGTACC 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 489
AclWI GGATC 1 cut(s) 760
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 2 cut(s) 306, 746
AfaI GTAC 2 cut(s) 379, 384
AfiI CCNNNNNNNGG 1 cut(s) 489
AflII CTTAAG 2 cut(s) 137, 227
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 6 cut(s) 53, 527, 571, 672, 734, 950
AjnI CCWGG 2 cut(s) 264, 373
AluBI AGCT 7 cut(s) 187, 232, 242, 401, 588, 1008, 1051
AluI AGCT 7 cut(s) 187, 232, 242, 401, 588, 1008, 1051
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 3 cut(s) 138, 977, 1045
AlwI GGATC 1 cut(s) 760
AoxI GGCC 3 cut(s) 491, 504, 757
ApoI RAATTY 1 cut(s) 471
Asp718I GGTACC 1 cut(s) 377
AspS9I GGNCC 3 cut(s) 492, 505, 787
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 842, 1031
AvaII GGWCC 1 cut(s) 787
BanI GGYRCC 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 721
BccI CCATC 1 cut(s) 704
BciT130I CCWGG 2 cut(s) 266, 375
BciVI GTATCC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 508
BcoDI GTCTC 3 cut(s) 138, 977, 1045
BfmI CTRYAG 1 cut(s) 1024
BfrI CTTAAG 2 cut(s) 137, 227
BfuI GTATCC 1 cut(s) 372
Bme1390I CCNGG 3 cut(s) 266, 375, 508
Bme18I GGWCC 1 cut(s) 787
BmgT120I GGNCC 3 cut(s) 492, 505, 787
BmiI GGNNCC 5 cut(s) 6, 372, 379, 788, 789
BmrFI CCNGG 3 cut(s) 266, 375, 508
BmrI ACTGGG 1 cut(s) 262
BmuI ACTGGG 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 255
BpuMI CCSGG 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 2 cut(s) 451, 1054
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 1025, 1055
Bsc4I CCNNNNNNNGG 1 cut(s) 489
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 257
BseBI CCWGG 2 cut(s) 266, 375
BseDI CCNNGG 2 cut(s) 451, 1054
BseGI GGATG 2 cut(s) 460, 654
BseLI CCNNNNNNNGG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 2 cut(s) 132, 362
BshFI GGCC 3 cut(s) 493, 506, 759
BshNI GGYRCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 721
BsiSI CCGG 2 cut(s) 503, 508
BslFI GGGAC 2 cut(s) 773, 1051
BslI CCNNNNNNNGG 1 cut(s) 489
BsmAI GTCTC 3 cut(s) 138, 977, 1045
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 773, 1051
BsnI GGCC 3 cut(s) 493, 506, 759
Bso31I GGTCTC 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 721
Bsp143I GATC 4 cut(s) 175, 418, 679, 765
BspANI GGCC 3 cut(s) 493, 506, 759
BspLI GGNNCC 5 cut(s) 6, 372, 379, 788, 789
BspPI GGATC 1 cut(s) 760
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 2 cut(s) 137, 227
BspTNI GGTCTC 1 cut(s) 977
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 2 cut(s) 451, 1054
BssMI GATC 4 cut(s) 175, 418, 679, 765
BssT1I CCWWGG 2 cut(s) 451, 1054
Bst2UI CCWGG 2 cut(s) 266, 375
Bst4CI ACNGT 3 cut(s) 382, 727, 749
Bst6I CTCTTC 3 cut(s) 99, 597, 807
BstAFI CTTAAG 2 cut(s) 137, 227
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 4 cut(s) 183, 688, 695, 848
BstF5I GGATG 2 cut(s) 460, 654
BstKTI GATC 4 cut(s) 178, 421, 682, 768
BstMAI GTCTC 3 cut(s) 138, 977, 1045
BstMBI GATC 4 cut(s) 175, 418, 679, 765
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNI CCWGG 2 cut(s) 266, 375
BstNSI RCATGY 1 cut(s) 445
BstSCI CCNGG 3 cut(s) 264, 373, 506
BstSFI CTRYAG 1 cut(s) 1024
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 765
BstYI RGATCY 1 cut(s) 765
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 3 cut(s) 493, 506, 759
BtsCI GGATG 2 cut(s) 460, 654
BtsI GCAGTG 2 cut(s) 639, 648
BtsIMutI CAGTG 4 cut(s) 520, 639, 648, 723
Cac8I GCNNGC 1 cut(s) 639
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 492, 505, 787
Csp6I GTAC 2 cut(s) 378, 383
CviAII CATG 5 cut(s) 278, 442, 666, 993, 1016
CviQI GTAC 2 cut(s) 378, 383
DdeI CTNAG 4 cut(s) 183, 688, 695, 848
DpnI GATC 4 cut(s) 177, 420, 681, 767
DpnII GATC 4 cut(s) 175, 418, 679, 765
Eam1104I CTCTTC 3 cut(s) 99, 597, 807
EarI CTCTTC 3 cut(s) 99, 597, 807
Eco130I CCWWGG 2 cut(s) 451, 1054
Eco31I GGTCTC 1 cut(s) 977
Eco47I GGWCC 1 cut(s) 787
Eco57I CTGAAG 2 cut(s) 306, 746
EcoO109I RGGNCCY 1 cut(s) 787
EcoRII CCWGG 2 cut(s) 264, 373
EcoT14I CCWWGG 2 cut(s) 451, 1054
ErhI CCWWGG 2 cut(s) 451, 1054
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 5 cut(s) 281, 445, 669, 996, 1019
FaqI GGGAC 2 cut(s) 773, 1051
FatI CATG 5 cut(s) 277, 441, 665, 992, 1015
FokI GGATG 2 cut(s) 447, 661
HaeIII GGCC 3 cut(s) 493, 506, 759
HapII CCGG 2 cut(s) 503, 508
Hin1II CATG 5 cut(s) 281, 445, 669, 996, 1019
HindIII AAGCTT 3 cut(s) 240, 399, 586
HinfI GANTC 6 cut(s) 93, 120, 142, 163, 223, 1084
HpaII CCGG 2 cut(s) 503, 508
HphI GGTGA 2 cut(s) 842, 1031
Hpy166II GTNNAC 1 cut(s) 701
Hpy188I TCNGA 6 cut(s) 162, 325, 415, 423, 709, 765
Hpy188III TCNNGA 4 cut(s) 53, 86, 524, 677
Hpy8I GTNNAC 1 cut(s) 701
HpyAV CCTTC 2 cut(s) 521, 770
HpyCH4III ACNGT 3 cut(s) 382, 727, 749
HpyCH4V TGCA 5 cut(s) 438, 558, 637, 932, 996
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 4 cut(s) 183, 688, 695, 848
Hsp92II CATG 5 cut(s) 281, 445, 669, 996, 1019
KflI GGGWCCC 1 cut(s) 787
KpnI GGTACC 1 cut(s) 381
Kzo9I GATC 4 cut(s) 175, 418, 679, 765
LmnI GCTCC 3 cut(s) 10, 370, 1056
MaeIII GTNAC 4 cut(s) 530, 886, 919, 1019
MalI GATC 4 cut(s) 177, 420, 681, 767
MboI GATC 4 cut(s) 175, 418, 679, 765
MflI RGATCY 1 cut(s) 765
MhlI GDGCHC 1 cut(s) 721
MluCI AATT 3 cut(s) 471, 652, 1090
MlyI GAGTC 2 cut(s) 129, 136
MmeI TCCRAC 1 cut(s) 1068
MseI TTAA 4 cut(s) 138, 228, 795, 1093
MslI CAYNNNNRTG 2 cut(s) 708, 842
MspCI CTTAAG 2 cut(s) 137, 227
MspI CCGG 2 cut(s) 503, 508
MspR9I CCNGG 3 cut(s) 266, 375, 508
MvaI CCWGG 2 cut(s) 266, 375
MwoI GCNNNNNNNGC 1 cut(s) 638
NciI CCSGG 1 cut(s) 508
NdeII GATC 4 cut(s) 175, 418, 679, 765
NlaIII CATG 5 cut(s) 281, 445, 669, 996, 1019
NlaIV GGNNCC 5 cut(s) 6, 372, 379, 788, 789
NmuCI GTSAC 2 cut(s) 919, 1019
NspI RCATGY 1 cut(s) 445
PciI ACATGT 1 cut(s) 441
PfeI GAWTC 4 cut(s) 93, 163, 223, 1084
PflFI GACNNNGTC 1 cut(s) 696
PflMI CCANNNNNTGG 1 cut(s) 489
PleI GAGTC 2 cut(s) 128, 136
PpsI GAGTC 2 cut(s) 128, 136
PpuMI RGGWCCY 1 cut(s) 787
PscI ACATGT 1 cut(s) 441
PsiI TTATAA 1 cut(s) 272
Psp5II RGGWCCY 1 cut(s) 787
Psp6I CCWGG 2 cut(s) 264, 373
PspGI CCWGG 2 cut(s) 264, 373
PspN4I GGNNCC 5 cut(s) 6, 372, 379, 788, 789
PspPI GGNCC 3 cut(s) 492, 505, 787
PspPPI RGGWCCY 1 cut(s) 787
PsuI RGATCY 1 cut(s) 765
PsyI GACNNNGTC 1 cut(s) 696
RsaI GTAC 2 cut(s) 379, 384
RsaNI GTAC 2 cut(s) 378, 383
RseI CAYNNNNRTG 2 cut(s) 708, 842
SaqAI TTAA 4 cut(s) 138, 228, 795, 1093
Sau3AI GATC 4 cut(s) 175, 418, 679, 765
Sau96I GGNCC 3 cut(s) 492, 505, 787
SchI GAGTC 2 cut(s) 129, 136
ScrFI CCNGG 3 cut(s) 266, 375, 508
SduI GDGCHC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 1024
SinI GGWCC 1 cut(s) 787
SmiMI CAYNNNNRTG 2 cut(s) 708, 842
SmlI CTYRAG 2 cut(s) 137, 227
SmoI CTYRAG 2 cut(s) 137, 227
Sse9I AATT 3 cut(s) 471, 652, 1090
StyD4I CCNGG 3 cut(s) 264, 373, 506
StyI CCWWGG 2 cut(s) 451, 1054
TaaI ACNGT 3 cut(s) 382, 727, 749
TaqI TCGA 1 cut(s) 23
TasI AATT 3 cut(s) 471, 652, 1090
TfiI GAWTC 4 cut(s) 93, 163, 223, 1084
Tru1I TTAA 4 cut(s) 138, 228, 795, 1093
Tru9I TTAA 4 cut(s) 138, 228, 795, 1093
TscAI CASTG 4 cut(s) 520, 639, 648, 730
TseFI GTSAC 2 cut(s) 919, 1019
Tsp45I GTSAC 2 cut(s) 919, 1019
TspDTI ATGAA 5 cut(s) 85, 143, 394, 449, 665
TspRI CASTG 4 cut(s) 520, 639, 648, 730
Tth111I GACNNNGTC 1 cut(s) 696
Van91I CCANNNNNTGG 1 cut(s) 489
Vha464I CTTAAG 2 cut(s) 137, 227
VpaK11BI GGWCC 1 cut(s) 787
XapI RAATTY 1 cut(s) 471
XceI RCATGY 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.