Rorug04G0174900

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014234
Physical Location & Seq
Forward (+)
30702408 .. 30713030
10623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0174900.1

Sequence Viewer

Length: 1068 bp
ATGGGGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCATGGAGGTGTCAGCTCAAGGACTGGGCTGCATGGGCATGTCTGCCTTCTATGGCCCCCCGAAGCCTGAAGCCGACATGATCAAGCTCATCCACCACGCCGTTGACTCCGGCATCACCTTCCTCGACACCTCCGACAACTACGGTCCGCACACCAATGAAATTCTCCTCGGCAAGGTTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTCGGTATAAGGTTTGCGGACAACAAAATGGAGATTCGAGGCGACCCGGCATATGTGAGAGCTGCTTGTGAAGGAAGCTTGAAGCGGCTCGGTGTAGATTCTATTGATCTCTATTATCAGCATAGGATTGACACCCGTGTCCCCATTGAAGTCACGGTGGGGGAGCTCAAGAAGCTAATTGAAGAGGGTAAAATAAAGTATATTGGTCTGTCTGAGGCATCAGCTTCAACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGATGTAGAGGAAGAAATAAATAATCCCTTCTTGTCGCATTTCATTGATATTACTGTCCCAAATTTCTCCAGGGAACTAGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCATTTCATCGGGGGCAAATTTCATCGAAAATCTTCCCAATGATGATTATCGAAAGAATATGCCCAGGTTCCAAGCTGGAAACCTAGAGCATAATAAAACAATATTTGTGCGAGTAAGTGATCTTGCAGCAAGGAAGGGTTGCACCCCATCTCAACTAGCACTGGCCTGGGTTCATCACCAGGGAAATGATGTGTGTCCCATACCCGGAACCACCAAGATTGAGAATTTAAACCAGAATATTGGAGCTCTGTCTGTGAACCTCACACCAGAAGAATTGGTTGAGCTTGAATCCTTTGCTTCTGCGGATTCTGTGAAGGGCGACCGATATCAAAATGGTATCAGTACTTGGATGAACTCTGAAACTCCACCACTGTCTTCATGGAAAGCCACATAA

Protein Analysis

355

Amino Acids

39.04

Weight (kDa)

6.08

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 323 8.2e-63 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014234)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21420
fragaria_vesca FvH4_4g17720 FvH4_4g17720
malus_domestica MD13G1170400.v1.1 MD16G1172000.v1.1
prunus_persica Prupe.1G138800_v2.0.a1
pyrus_communis pycom13g14590
rosa_chinensis RchiOBHm_Chr4g0421691
rosa_laevigata RLG00000007658
rosa_multiflora Rmu_sc0004533.1_g000010
rosa_roxburghii Rroxscaffold_5G00363930
rosa_rugosa Rorug04G0174700 Rorug04G0174800 Rorug04G0174900
rosa_samantha Rh4CG248900 Rh4DG232800
rosa_wichuraiana Rw4G020000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AciI CCGC 4 cut(s) 185, 275, 343, 977
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 5 cut(s) 198, 257, 616, 691, 898
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 1018
AfiI CCNNNNNNNGG 2 cut(s) 211, 878
AgsI TTSAA 6 cut(s) 245, 340, 407, 440, 486, 962
AjnI CCWGG 4 cut(s) 623, 737, 839, 852
AjuI GAANNNNNNNTTGG 2 cut(s) 621, 653
Alw21I GWGCWC 2 cut(s) 426, 922
Alw26I GTCTC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 3 cut(s) 91, 249, 837
ApeKI GCWGC 4 cut(s) 66, 320, 529, 800
ApoI RAATTY 5 cut(s) 198, 257, 616, 691, 898
Asp700I GAANNNNTTC 1 cut(s) 705
AspS9I GGNCC 3 cut(s) 92, 182, 540
AsuC2I CCSGG 2 cut(s) 305, 879
AsuHPI GGTGA 3 cut(s) 145, 241, 842
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 182, 540
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 3 cut(s) 426, 502, 922
BbsI GAAGAC 1 cut(s) 1041
Bbv12I GWGCWC 2 cut(s) 426, 922
BbvI GCAGC 4 cut(s) 53, 307, 541, 812
BccI CCATC 1 cut(s) 829
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 4 cut(s) 625, 739, 841, 854
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 305, 879
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 4 cut(s) 632, 662, 758, 830
BisI GCNGC 5 cut(s) 67, 321, 344, 530, 801
BlsI GCNGC 5 cut(s) 68, 322, 345, 531, 802
BmcAI AGTACT 1 cut(s) 1018
Bme1390I CCNGG 6 cut(s) 305, 625, 739, 841, 854, 879
Bme18I GGWCC 2 cut(s) 182, 540
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 92, 182, 540
BmiI GGNNCC 3 cut(s) 94, 743, 883
BmrFI CCNGG 6 cut(s) 305, 625, 739, 841, 854, 879
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 159, 485
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 1041
BpmI CTGGAG 2 cut(s) 554, 607
BpuEI CTTGAG 2 cut(s) 39, 410
BpuMI CCSGG 2 cut(s) 305, 879
BsaBI GATNNNNATC 1 cut(s) 720
BsaJI CCNNGG 5 cut(s) 205, 624, 737, 840, 853
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 878
Bse1I ACTGG 2 cut(s) 66, 840
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 4 cut(s) 625, 739, 841, 854
BseDI CCNNGG 5 cut(s) 205, 624, 737, 840, 853
BseGI GGATG 4 cut(s) 24, 126, 511, 1029
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 2 cut(s) 211, 878
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 2 cut(s) 66, 840
BseRI GAGGAG 2 cut(s) 28, 194
BseXI GCAGC 4 cut(s) 53, 307, 541, 812
BsgI GTGCAG 1 cut(s) 548
Bsh1285I CGRYCG 1 cut(s) 997
BshFI GGCC 3 cut(s) 93, 251, 839
BsiEI CGRYCG 1 cut(s) 997
BsiHKAI GWGCWC 2 cut(s) 426, 922
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 147, 305, 879
BslFI GGGAC 3 cut(s) 383, 596, 855
BslI CCNNNNNNNGG 2 cut(s) 211, 878
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 3 cut(s) 383, 596, 855
BsnI GGCC 3 cut(s) 93, 251, 839
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 3 cut(s) 426, 502, 922
Bsp143I GATC 3 cut(s) 117, 364, 793
BspACI CCGC 4 cut(s) 185, 275, 343, 977
BspANI GGCC 3 cut(s) 93, 251, 839
BspCNI CTCAG 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 94, 743, 883
BspQI GCTCTTC 1 cut(s) 490
BsrI ACTGG 2 cut(s) 66, 840
BssECI CCNNGG 5 cut(s) 205, 624, 737, 840, 853
BssMI GATC 3 cut(s) 117, 364, 793
Bst2UI CCWGG 4 cut(s) 625, 739, 841, 854
Bst4CI ACNGT 5 cut(s) 182, 415, 610, 656, 1047
Bst6I CTCTTC 3 cut(s) 435, 490, 661
BstDEI CTNAG 1 cut(s) 471
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 4 cut(s) 24, 126, 511, 1029
BstKTI GATC 3 cut(s) 120, 367, 796
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 3 cut(s) 117, 364, 793
BstMCI CGRYCG 1 cut(s) 997
BstMWI GCNNNNNNNGC 2 cut(s) 72, 430
BstNI CCWGG 4 cut(s) 625, 739, 841, 854
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 6 cut(s) 303, 623, 737, 839, 852, 877
BstV1I GCAGC 4 cut(s) 53, 307, 541, 812
BstV2I GAAGAC 1 cut(s) 1041
BstXI CCANNNNNNTGG 1 cut(s) 914
BsuRI GGCC 3 cut(s) 93, 251, 839
BtsCI GGATG 4 cut(s) 24, 126, 511, 1029
BtsIMutI CAGTG 2 cut(s) 833, 1043
Cfr13I GGNCC 3 cut(s) 92, 182, 540
CpoI CGGWCCG 1 cut(s) 182
Csp6I GTAC 1 cut(s) 1017
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CspI CGGWCCG 1 cut(s) 182
CviAII CATG 6 cut(s) 40, 70, 76, 115, 503, 1053
CviQI GTAC 1 cut(s) 1017
DdeI CTNAG 1 cut(s) 471
DpnI GATC 3 cut(s) 119, 366, 795
DpnII GATC 3 cut(s) 117, 364, 793
DraI TTTAAA 1 cut(s) 903
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 3 cut(s) 435, 490, 661
EarI CTCTTC 3 cut(s) 435, 490, 661
Ecl136II GAGCTC 2 cut(s) 424, 920
Eco24I GRGCYC 3 cut(s) 426, 502, 922
Eco32I GATATC 1 cut(s) 1001
Eco47I GGWCC 2 cut(s) 182, 540
Eco53kI GAGCTC 2 cut(s) 424, 920
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 2 cut(s) 424, 920
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 623, 737, 839, 852
EcoRV GATATC 1 cut(s) 1001
EcoT38I GRGCYC 3 cut(s) 426, 502, 922
FaeI CATG 6 cut(s) 43, 73, 79, 118, 506, 1056
FaqI GGGAC 3 cut(s) 383, 596, 855
FatI CATG 6 cut(s) 39, 69, 75, 114, 502, 1052
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 5 cut(s) 67, 321, 344, 530, 801
FokI GGATG 4 cut(s) 31, 113, 498, 1036
FriOI GRGCYC 3 cut(s) 426, 502, 922
Fsp4HI GCNGC 5 cut(s) 67, 321, 344, 530, 801
FspBI CTAG 4 cut(s) 632, 662, 758, 830
GluI GCNGC 5 cut(s) 67, 321, 344, 530, 801
GsuI CTGGAG 2 cut(s) 554, 607
HaeIII GGCC 3 cut(s) 93, 251, 839
HapII CCGG 3 cut(s) 147, 305, 879
Hin1II CATG 6 cut(s) 43, 73, 79, 118, 506, 1056
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 6 cut(s) 143, 292, 356, 672, 962, 980
HpaII CCGG 3 cut(s) 147, 305, 879
HphI GGTGA 3 cut(s) 145, 241, 842
Hpy166II GTNNAC 2 cut(s) 142, 931
Hpy188I TCNGA 3 cut(s) 172, 472, 1033
Hpy188III TCNNGA 2 cut(s) 427, 552
Hpy8I GTNNAC 2 cut(s) 142, 931
HpyAV CCTTC 6 cut(s) 94, 166, 323, 592, 802, 982
HpyCH4III ACNGT 5 cut(s) 182, 415, 610, 656, 1047
HpyCH4V TGCA 5 cut(s) 69, 529, 650, 800, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 430
HpyF3I CTNAG 1 cut(s) 471
Hsp92II CATG 6 cut(s) 43, 73, 79, 118, 506, 1056
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 364, 793
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 2 cut(s) 421, 917
Lsp1109I GCAGC 4 cut(s) 53, 307, 541, 812
LweI GCATC 2 cut(s) 159, 485
MaeI CTAG 4 cut(s) 632, 662, 758, 830
MaeIII GTNAC 2 cut(s) 30, 409
MalI GATC 3 cut(s) 119, 366, 795
MboI GATC 3 cut(s) 117, 364, 793
MboII GAAGA 7 cut(s) 452, 507, 578, 678, 698, 956, 1041
MhlI GDGCHC 3 cut(s) 426, 502, 922
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 8 cut(s) 198, 245, 257, 435, 616, 691, 898, 947
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MroXI GAANNNNTTC 1 cut(s) 705
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 902
MslI CAYNNNNRTG 3 cut(s) 44, 74, 192
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 3 cut(s) 147, 305, 879
MspR9I CCNGG 6 cut(s) 305, 625, 739, 841, 854, 879
MvaI CCWGG 4 cut(s) 625, 739, 841, 854
MwoI GCNNNNNNNGC 2 cut(s) 72, 430
NciI CCSGG 2 cut(s) 305, 879
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 117, 364, 793
NlaIII CATG 6 cut(s) 43, 73, 79, 118, 506, 1056
NlaIV GGNNCC 3 cut(s) 94, 743, 883
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 30, 409
NspI RCATGY 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 5 cut(s) 292, 356, 672, 962, 980
PkrI GCNGC 5 cut(s) 68, 322, 345, 531, 802
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 2 cut(s) 426, 922
Psp6I CCWGG 4 cut(s) 623, 737, 839, 852
PspGI CCWGG 4 cut(s) 623, 737, 839, 852
PspN4I GGNNCC 3 cut(s) 94, 743, 883
PspPI GGNCC 3 cut(s) 92, 182, 540
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 1 cut(s) 1018
RsaNI GTAC 1 cut(s) 1017
RseI CAYNNNNRTG 3 cut(s) 44, 74, 192
Rsr2I CGGWCCG 1 cut(s) 182
RsrII CGGWCCG 1 cut(s) 182
SacI GAGCTC 2 cut(s) 426, 922
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 1 cut(s) 902
SatI GCNGC 5 cut(s) 67, 321, 344, 530, 801
Sau3AI GATC 3 cut(s) 117, 364, 793
Sau96I GGNCC 3 cut(s) 92, 182, 540
ScaI AGTACT 1 cut(s) 1018
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 6 cut(s) 305, 625, 739, 841, 854, 879
SduI GDGCHC 3 cut(s) 426, 502, 922
SfaNI GCATC 2 cut(s) 159, 485
SinI GGWCC 2 cut(s) 182, 540
SmiMI CAYNNNNRTG 3 cut(s) 44, 74, 192
SmlI CTYRAG 2 cut(s) 54, 425
SmoI CTYRAG 2 cut(s) 54, 425
Sse9I AATT 8 cut(s) 198, 245, 257, 435, 616, 691, 898, 947
SsiI CCGC 4 cut(s) 185, 275, 343, 977
SspI AATATT 2 cut(s) 777, 913
SspMI CTAG 4 cut(s) 632, 662, 758, 830
SstI GAGCTC 2 cut(s) 426, 922
StyD4I CCNGG 6 cut(s) 303, 623, 737, 839, 852, 877
TaaI ACNGT 5 cut(s) 182, 415, 610, 656, 1047
TaqI TCGA 4 cut(s) 162, 295, 699, 724
TaqII GACCGA 1 cut(s) 1011
TasI AATT 8 cut(s) 198, 245, 257, 435, 616, 691, 898, 947
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 5 cut(s) 292, 356, 672, 962, 980
Tru1I TTAA 1 cut(s) 902
Tru9I TTAA 1 cut(s) 902
TscAI CASTG 2 cut(s) 840, 1050
TseFI GTSAC 2 cut(s) 30, 409
TseI GCWGC 4 cut(s) 66, 320, 529, 800
Tsp45I GTSAC 2 cut(s) 30, 409
TspRI CASTG 2 cut(s) 840, 1050
VpaK11BI GGWCC 2 cut(s) 182, 540
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 5 cut(s) 198, 257, 616, 691, 898
XceI RCATGY 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 1050
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 4 cut(s) 632, 662, 758, 830
ZrmI AGTACT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.