AT3G22104

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004787
Physical Location & Seq
Forward (+)
7789342 .. 7792410
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22104.4

Sequence Viewer

Length: 1521 bp
ATGGAAGCTTGTTGCTCTGATCTTGAAGTAGACATCAATGGAGAACAAACTATCTTTCTCAATAAGCAAATCATATGTGCTTATTCTGGAACGTTAAGAAAGCTTTTGGGGAAATCAACTAGTTCCAGTGGAAATCTCAAGGTGATCTTCAATGATTTCCCCGGTGGAGCTGAGAGTTTCGAGTTTGTATCGAGGTTTTGCTACAATGACGGTCGAGTAGCTGTGATGCCTTCGAATGTAGTTTTCTTGCATTGTGCTGCTAAGTTTATGGAAGTCACTAAAGTCTTGGAACAAACAGAGAAGTGTATGGAAGAGATTAGGTATTGGGCTTGGCCAGAGGTTCTTCTTTGTCTAAAACAGTGTCAAGAAGTTGAAACATCGCCCGAAGTTGATTCTTTAGCCGCGAAACTGATGGATGCGTTGGTGGAGAAACTGTGTTTAACCATTGAAATGAGTCCTTCTAGTGCTGGTTCTGCTTGTTCACCAGATAGCAGCTTGTTTCGGTTTTCTTGTGATAGTAAAAGCACAGAGAGTTTCAAGAATTGCTCTGTTCGGTTAACTTGGTGGTTCGATGAAGTTCTTGTTCTGAGTTCTGGTTTGGTTGAAATGTTCTTGAAGCTTATGGTATTGCGGAAATTTGATAATCTCATCATAAGTAGGTTCTTGTTCTATTATCAGAAGGTCAAGTTTTGCTCTGCGTCTTCTCATGAGAAAAGAAAGATTCTTGAAACCATCATTGATACTCTTTGCGTCTTAGATCGAAGTTGTGTCCCTTGCAAGAGCCTTTTTGCGGTTTTGAGGCTTGCTCTTGGATTGAACATAAACAAAAGCTGTATGAACAAGCTGGAGGTTATGATAGGTCACCAACTGGATCAAGCAACACTAGACAATCTTCTTGTTCCATCTCCATCGAAATCGAGTCACTTGTACTATGTGAATCTTGTTCTTAGATTCACAAAAGCTTTCCTAGATGGGGCAAGAAGTGGATTGCAATTGAAGAAAGTCTCAAGTTTGATTGATCAATACATAGCTGAAGTAGCACCTGATCCTTGCTTGAAACCTTCAAAGTTTCTTTCTCTCATCACACTTGTTCCAGATTCAGCCAGAGAATCCCACGAAGATATATACCGTGCTATTGATATGTATCTCGAGGCTCACACAGGAACAACTGATGGCGAAAAACTCAACTTGATACGAACTTTAAGTTACGAGAAGCTTTCAGGAGAATCAAGAGCTCACATTTCGCGTAACCAAAAGTTTCAGGCTATTGAGACACTAGATGAACAACAACAACAACAACAACAACAACAACAACAGAAGCAGCTCATACTCAGAATGGAGAAGGTTGAGACTTCAGGAGAAAACGAGAAGCTGAAGGAACATATCGAAGGGATTCAATGGAGGGTAATGGAATTGGAGAGAGCTTGTTTGAAAATGCAGAATCAAATGGAAGTTATCAAGAAGAGATCCAAGAGCTCGAGCAAAGGAAGTAATAGATCACTTCCTAAGCTCTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

506

Amino Acids

57.38

Weight (kDa)

7.41

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 112 - 334 1.3e-55 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 30
AccII CGCG 2 cut(s) 404, 1246
AciI CCGC 3 cut(s) 402, 631, 791
AclI AACGTT 1 cut(s) 92
AclWI GGATC 3 cut(s) 879, 1040, 1461
AcoI YGGCCR 1 cut(s) 332
AcsI RAATTY 1 cut(s) 635
AcuI CTGAAG 3 cut(s) 1053, 1338, 1394
AfaI GTAC 1 cut(s) 929
AfiI CCNNNNNNNGG 2 cut(s) 790, 973
AhlI ACTAGT 1 cut(s) 119
Alw21I GWGCWC 2 cut(s) 1237, 1478
Alw26I GTCTC 3 cut(s) 1009, 1265, 1343
AlwI GGATC 3 cut(s) 879, 1040, 1461
Ama87I CYCGRG 2 cut(s) 1148, 1477
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 3 cut(s) 257, 492, 1321
ApoI RAATTY 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 1392
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 3 cut(s) 154, 474, 854
AsuII TTCGAA 1 cut(s) 233
AvaI CYCGRG 2 cut(s) 1148, 1477
BalI TGGCCA 1 cut(s) 334
BanII GRGCYC 2 cut(s) 1237, 1478
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 1237, 1478
BbvI GCAGC 3 cut(s) 244, 504, 1333
BccI CCATC 6 cut(s) 406, 740, 910, 916, 965, 1166
BclI TGATCA 1 cut(s) 1018
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 3 cut(s) 1009, 1265, 1343
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 5 cut(s) 120, 462, 884, 968, 1277
BisI GCNGC 4 cut(s) 258, 402, 493, 1322
BlsI GCNGC 4 cut(s) 259, 403, 494, 1323
Bme1390I CCNGG 1 cut(s) 162
BmeT110I CYCGRG 2 cut(s) 1148, 1477
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 216, 406
BpiI GAAGAC 1 cut(s) 693
BplI GAGNNNNNCTC 2 cut(s) 790, 822
BpmI CTGGAG 1 cut(s) 866
Bpu10I CCTNAGC 1 cut(s) 1506
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 2 cut(s) 122, 991
BpuMI CCSGG 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 1143
BsaJI CCNNGG 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 790, 973
Bse1I ACTGG 2 cut(s) 126, 873
Bse8I GATNNNNATC 1 cut(s) 1143
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 421
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 2 cut(s) 790, 973
BseMII CTCAG 3 cut(s) 162, 578, 1345
BseNI ACTGG 2 cut(s) 126, 873
BseXI GCAGC 3 cut(s) 244, 504, 1333
Bsh1236I CGCG 2 cut(s) 404, 1246
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 1 cut(s) 334
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 2 cut(s) 1237, 1478
BsiHKCI CYCGRG 2 cut(s) 1148, 1477
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 2 cut(s) 790, 973
BsmAI GTCTC 3 cut(s) 1009, 1265, 1343
BsmFI GGGAC 1 cut(s) 755
BsnI GGCC 1 cut(s) 334
BsoBI CYCGRG 2 cut(s) 1148, 1477
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 1237, 1478
Bsp143I GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
BspACI CCGC 3 cut(s) 402, 631, 791
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 3 cut(s) 163, 579, 1344
BspFNI CGCG 2 cut(s) 404, 1246
BspHI TCATGA 1 cut(s) 706
BspPI GGATC 3 cut(s) 879, 1040, 1461
BspT104I TTCGAA 1 cut(s) 233
BsrI ACTGG 2 cut(s) 126, 873
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
Bst4CI ACNGT 4 cut(s) 212, 360, 435, 1130
Bst6I CTCTTC 2 cut(s) 306, 1457
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 7 cut(s) 171, 261, 587, 754, 947, 1331, 1506
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 2 cut(s) 404, 1246
BstKTI GATC 8 cut(s) 22, 147, 760, 874, 1021, 1048, 1469, 1499
BstMAI GTCTC 3 cut(s) 1009, 1265, 1343
BstMBI GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 473, 1037
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 1 cut(s) 160
BstUI CGCG 2 cut(s) 404, 1246
BstV1I GCAGC 3 cut(s) 244, 504, 1333
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 1466
BstYI RGATCY 1 cut(s) 1466
BsuRI GGCC 1 cut(s) 334
BtgZI GCGATG 1 cut(s) 363
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 2 cut(s) 133, 365
Cac8I GCNNGC 1 cut(s) 804
CciI TCATGA 1 cut(s) 706
CseI GACGC 2 cut(s) 687, 739
Csp6I GTAC 1 cut(s) 928
CviAII CATG 1 cut(s) 707
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 7 cut(s) 171, 261, 587, 754, 947, 1331, 1506
DpnI GATC 8 cut(s) 21, 146, 759, 873, 1020, 1047, 1468, 1498
DpnII GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 306, 1457
EarI CTCTTC 2 cut(s) 306, 1457
Ecl136II GAGCTC 2 cut(s) 1235, 1476
Eco24I GRGCYC 2 cut(s) 1237, 1478
Eco53kI GAGCTC 2 cut(s) 1235, 1476
Eco57I CTGAAG 3 cut(s) 1053, 1338, 1394
Eco88I CYCGRG 2 cut(s) 1148, 1477
Eco91I GGTNACC 1 cut(s) 860
EcoICRI GAGCTC 2 cut(s) 1235, 1476
EcoO65I GGTNACC 1 cut(s) 860
EcoT38I GRGCYC 2 cut(s) 1237, 1478
FaeI CATG 1 cut(s) 710
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FaqI GGGAC 1 cut(s) 755
FatI CATG 1 cut(s) 706
FauNDI CATATG 1 cut(s) 74
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 4 cut(s) 258, 402, 493, 1322
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 2 cut(s) 1237, 1478
Fsp4HI GCNGC 4 cut(s) 258, 402, 493, 1322
FspBI CTAG 5 cut(s) 120, 462, 884, 968, 1277
GluI GCNGC 4 cut(s) 258, 402, 493, 1322
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 1 cut(s) 334
HapII CCGG 1 cut(s) 162
HgaI GACGC 2 cut(s) 687, 739
Hin1II CATG 1 cut(s) 710
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HindIII AAGCTT 5 cut(s) 6, 101, 617, 960, 1214
HpaI GTTAAC 1 cut(s) 558
HpaII CCGG 1 cut(s) 162
HphI GGTGA 3 cut(s) 154, 474, 854
Hpy166II GTNNAC 3 cut(s) 31, 482, 558
Hpy188I TCNGA 4 cut(s) 19, 588, 678, 1334
Hpy8I GTNNAC 3 cut(s) 31, 482, 558
HpyAV CCTTC 7 cut(s) 240, 468, 673, 1071, 1336, 1369, 1382
HpyCH4III ACNGT 4 cut(s) 212, 360, 435, 1130
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 250, 777, 991, 1438
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 1037
HpyF3I CTNAG 7 cut(s) 171, 261, 587, 754, 947, 1331, 1506
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 1 cut(s) 710
Ksp22I TGATCA 1 cut(s) 1018
KspAI GTTAAC 1 cut(s) 558
Kzo9I GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 3 cut(s) 244, 504, 1333
LweI GCATC 2 cut(s) 216, 406
MaeI CTAG 5 cut(s) 120, 462, 884, 968, 1277
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 5 cut(s) 274, 860, 920, 1205, 1247
MalI GATC 8 cut(s) 21, 146, 759, 873, 1020, 1047, 1468, 1498
MboI GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
MboII GAAGA 8 cut(s) 139, 323, 335, 693, 884, 1009, 1130, 1474
MfeI CAATTG 1 cut(s) 992
MflI RGATCY 1 cut(s) 1466
MhlI GDGCHC 2 cut(s) 1237, 1478
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 4 cut(s) 541, 635, 992, 1412
MluNI TGGCCA 1 cut(s) 334
MlyI GAGTC 2 cut(s) 463, 928
MnlI CCTC 6 cut(s) 186, 331, 792, 841, 1144, 1395
Mox20I TGGCCA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 1392
MscI TGGCCA 1 cut(s) 334
MseI TTAA 4 cut(s) 95, 440, 557, 1202
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 334
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 162
MunI CAATTG 1 cut(s) 992
MvnI CGCG 2 cut(s) 404, 1246
MwoI GCNNNNNNNGC 2 cut(s) 473, 1037
NciI CCSGG 1 cut(s) 162
NdeI CATATG 1 cut(s) 74
NdeII GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
NlaIII CATG 1 cut(s) 710
NmuCI GTSAC 3 cut(s) 274, 860, 920
NspV TTCGAA 1 cut(s) 233
PaeR7I CTCGAG 2 cut(s) 1148, 1477
PagI TCATGA 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 1392
PfeI GAWTC 9 cut(s) 392, 721, 937, 951, 1097, 1109, 1226, 1393, 1441
PkrI GCNGC 4 cut(s) 259, 403, 494, 1323
PleI GAGTC 2 cut(s) 462, 927
PpsI GAGTC 2 cut(s) 462, 927
Psp124BI GAGCTC 2 cut(s) 1237, 1478
Psp1406I AACGTT 1 cut(s) 92
PspEI GGTNACC 1 cut(s) 860
PspXI VCTCGAGB 1 cut(s) 1477
PsuI RGATCY 1 cut(s) 1466
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 1 cut(s) 449
SacI GAGCTC 2 cut(s) 1237, 1478
SaqAI TTAA 4 cut(s) 95, 440, 557, 1202
SatI GCNGC 4 cut(s) 258, 402, 493, 1322
Sau3AI GATC 8 cut(s) 19, 144, 757, 871, 1018, 1045, 1466, 1496
SchI GAGTC 2 cut(s) 463, 928
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 2 cut(s) 1237, 1478
SfaNI GCATC 2 cut(s) 216, 406
Sfr274I CTCGAG 2 cut(s) 1148, 1477
SfuI TTCGAA 1 cut(s) 233
SlaI CTCGAG 2 cut(s) 1148, 1477
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 4 cut(s) 137, 1006, 1148, 1477
SmoI CTYRAG 4 cut(s) 137, 1006, 1148, 1477
SpeI ACTAGT 1 cut(s) 119
Sse9I AATT 4 cut(s) 541, 635, 992, 1412
SsiI CCGC 3 cut(s) 402, 631, 791
SspMI CTAG 5 cut(s) 120, 462, 884, 968, 1277
SstI GAGCTC 2 cut(s) 1237, 1478
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 4 cut(s) 212, 360, 435, 1130
TaiI ACGT 1 cut(s) 95
TasI AATT 4 cut(s) 541, 635, 992, 1412
TatI WGTACW 1 cut(s) 927
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 9 cut(s) 392, 721, 937, 951, 1097, 1109, 1226, 1393, 1441
Tru1I TTAA 4 cut(s) 95, 440, 557, 1202
Tru9I TTAA 4 cut(s) 95, 440, 557, 1202
TscAI CASTG 2 cut(s) 133, 365
TseFI GTSAC 3 cut(s) 274, 860, 920
TseI GCWGC 3 cut(s) 257, 492, 1321
Tsp45I GTSAC 3 cut(s) 274, 860, 920
TspDTI ATGAA 3 cut(s) 588, 851, 1296
TspRI CASTG 2 cut(s) 133, 365
XapI RAATTY 1 cut(s) 635
XhoI CTCGAG 2 cut(s) 1148, 1477
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 1392
XspI CTAG 5 cut(s) 120, 462, 884, 968, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.