pycom09g02610

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004787
Physical Location & Seq
Reverse (-)
1951345 .. 1953667
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02610.1

Sequence Viewer

Length: 1749 bp
ATGGCTGCCTTAGCTTTTTGTTCTTTTGGTTCTGTCTTTTTAATTCAACAAATTGCACGCCTTTTCCGTTTTGCTTCATTCTCTCCCATTTATTGCTGCTGTTATGGGAATTGGGAGGCTCAAATCTTCACTTATTTTGATATGGACTCTGAGACTATTGCCTCCTACTGTGGAAAATTGAGCAAACTACTTGGTAAATCAAAAGTTTCCTCAAGAAATCTTAAAGTGGTATTCCATGATTTTCCCGGAGGAGCAGAGAGTTTCGAGCTGATTGCGAGGTTCTGCTACAACAATGGCAGCATTTACATAACTCCTTCCAACATTTCCCTCCTTTACTGTGCCGCGCAGTTCATGGAAATGAACAATTCTGTCGCTGGCAGACATAATTTGTTACAAAAAGCGGAGAATTCATTTGAAGAGATCGATCTGGAAAACATGCCCAGATATTGGTCATGGTCTGAGCTTTTGACGGCTTTGAAGCAGTGCCAGGATTTGCTATCGGTTGCAAATTCTTTGAGTCTAGTTGAGAAATGCTTGGATACCCTTGTTGCAAGATTGCCTTGGACAAGCGAAGCGAGTCCCTGTCCTTCGACTTCATCTCCTGACAGCTCTGGAATTCGGTTTTCGTGCGACACTAGAAGCACCGAGAGTTTGAAAACCACCTTGTCTCGAGCAACCTGGTGGTTCGAGGATCTTCTAGTTTTAGGTCCAAATTTGGTTGAAATGCTGGTCAAGGCCATGGTTTCGCGAAAACTCGATCATGTTATCATTAGTAGGTTCCTCTTTTACTACCAGAAATCGAAGTTTTACACTGTCAAATCTGATACCAAGCGCACCATGGCTGAAATGGTCATTGAGATGCTGTATATGCTTGATCAGAGCTGTGTTTCATGCAAGAGCTTATTTGGGATTCTTCGAGTTATGCTGAATTTGAACTTAAACAAGAGTATTAGAGATAAGTTGGAGAAAATGATCGGTTCACAGCTAGATCAAGCAACATTGGACAATTTGCTTGTTCCATCCCCACAAGGAATGAATTATTTGTATGATGTCAATCTTGTGCTACGGTTCTTGAAATCATTTCTGCATGACAGAGGAACCCGTCAAGCTGACAGGCCCCCAATGCTATTAAGGAAAGTTGCTGCTTTGGTTGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTGAAGCCTTCAAAGTTTCTGGCTTTAGCCATCGCGCTGCCGGATTCTGCGAGAGACTCTTATGATGAGCTTTACCATGCCATAGACATGTATCTACAGGTACACCCAGGACTTTCTGAAGACGAAAAGATGAAGATATGTTGTGTGTTGAACTATGAGAAGCTCTCAGCAGAGACTTGCGTACACCTTTCACAGAACACAAAGTTTTCATCAAAATCTGCAGTCCAAGCTCTTGTCTATCAGCAATCCAAGCTCAAAAGCTTACTCCACGCTACCAACAATCCCAAGTCGTACACTGACTCCCCTTATAGCGCCATTTCTGAGGCCGAAAGTAGGGGATTAAGGAAAGATGATGCCAATGTACAACTTGTGCTTTATGCCGGGAAAATCGATCTCTCAGATGACAATGAGAAGCTCAGAACACATCTGCAAGGCATGCAGTGCAGGGTCATGGAATTGGAAAAACTTTGCAAGAAAATGCAATCTCAAATGGCGAAGTTTACGAAATCGAAAGCACCAAGCCACTACCATACAAGATCTTTGCCCAAACTTTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

583

Amino Acids

65.92

Weight (kDa)

8.44

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 226 - 456 6.9e-66 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AccII CGCG 3 cut(s) 344, 748, 1223
AciI CCGC 2 cut(s) 342, 401
AclWI GGATC 2 cut(s) 699, 1175
AcsI RAATTY 5 cut(s) 406, 508, 615, 712, 928
AcuI CTGAAG 1 cut(s) 1326
AfaI GTAC 4 cut(s) 1290, 1371, 1481, 1551
AfiI CCNNNNNNNGG 2 cut(s) 447, 1521
AflIII ACRYGT 1 cut(s) 1275
AjnI CCWGG 3 cut(s) 486, 677, 1294
Alw26I GTCTC 4 cut(s) 146, 672, 1236, 1355
AlwI GGATC 2 cut(s) 699, 1175
Ama87I CYCGRG 1 cut(s) 669
AoxI GGCC 3 cut(s) 735, 1115, 1512
ApeKI GCWGC 5 cut(s) 5, 96, 297, 1142, 1225
ApoI RAATTY 5 cut(s) 406, 508, 615, 712, 928
AspLEI GCGC 4 cut(s) 346, 834, 1225, 1502
AspS9I GGNCC 2 cut(s) 707, 1116
AsuC2I CCSGG 2 cut(s) 246, 1570
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 1 cut(s) 707
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BbsI GAAGAC 1 cut(s) 1314
BbvI GCAGC 4 cut(s) 83, 309, 1129, 1212
BccI CCATC 2 cut(s) 1027, 1226
BceAI ACGGC 1 cut(s) 486
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 3 cut(s) 488, 679, 1296
BciVI GTATCC 1 cut(s) 532
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 2 cut(s) 246, 1570
BcoDI GTCTC 4 cut(s) 146, 672, 1236, 1355
BfaI CTAG 4 cut(s) 521, 636, 698, 986
BfmI CTRYAG 2 cut(s) 1283, 1407
BfoI RGCGCY 1 cut(s) 1503
BfuI GTATCC 1 cut(s) 532
BglII AGATCT 1 cut(s) 1724
BisI GCNGC 6 cut(s) 6, 97, 298, 342, 1143, 1226
BlsI GCNGC 6 cut(s) 7, 98, 299, 343, 1144, 1227
Bme1390I CCNGG 5 cut(s) 246, 488, 679, 1296, 1570
Bme18I GGWCC 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 2 cut(s) 707, 1116
BmiI GGNNCC 3 cut(s) 779, 1099, 1118
BmrFI CCNGG 5 cut(s) 246, 488, 679, 1296, 1570
BmsI GCATC 2 cut(s) 849, 1531
BpiI GAAGAC 1 cut(s) 1314
BplI GAGNNNNNCTC 2 cut(s) 1337, 1369
Bpu10I CCTNAGC 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 196
BpuMI CCSGG 2 cut(s) 246, 1570
Bsa29I ATCGAT 2 cut(s) 423, 1578
BsaBI GATNNNNATC 1 cut(s) 1053
BsaJI CCNNGG 4 cut(s) 560, 738, 837, 1294
BsaXI ACNNNNNCTCC 4 cut(s) 583, 613, 1472, 1502
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 1521
Bse8I GATNNNNATC 1 cut(s) 1053
BseBI CCWGG 3 cut(s) 488, 679, 1296
BseCI ATCGAT 2 cut(s) 423, 1578
BseDI CCNNGG 4 cut(s) 560, 738, 837, 1294
BseGI GGATG 1 cut(s) 1019
BseJI GATNNNNATC 1 cut(s) 1053
BseLI CCNNNNNNNGG 2 cut(s) 447, 1521
BseMII CTCAG 6 cut(s) 141, 450, 1368, 1500, 1599, 1618
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 4 cut(s) 83, 309, 1129, 1212
BsgI GTGCAG 1 cut(s) 1651
Bsh1236I CGCG 3 cut(s) 344, 748, 1223
BshFI GGCC 3 cut(s) 737, 1117, 1514
BshVI ATCGAT 2 cut(s) 423, 1578
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 3 cut(s) 246, 1229, 1569
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 447, 1521
BsmAI GTCTC 4 cut(s) 146, 672, 1236, 1355
BsmFI GGGAC 1 cut(s) 564
BsnI GGCC 3 cut(s) 737, 1117, 1514
BsoBI CYCGRG 1 cut(s) 669
Bsp1407I TGTACA 1 cut(s) 1549
Bsp19I CCATGG 2 cut(s) 738, 837
Bsp68I TCGCGA 1 cut(s) 748
BspACI CCGC 2 cut(s) 342, 401
BspANI GGCC 3 cut(s) 737, 1117, 1514
BspCNI CTCAG 6 cut(s) 142, 451, 1367, 1501, 1598, 1617
BspDI ATCGAT 2 cut(s) 423, 1578
BspFNI CGCG 3 cut(s) 344, 748, 1223
BspHI TCATGA 1 cut(s) 1745
BspLI GGNNCC 3 cut(s) 779, 1099, 1118
BspMAI CTGCAG 1 cut(s) 1411
BspPI GGATC 2 cut(s) 699, 1175
BsrGI TGTACA 1 cut(s) 1549
BssECI CCNNGG 4 cut(s) 560, 738, 837, 1294
BssT1I CCWWGG 3 cut(s) 560, 738, 837
Bst2UI CCWGG 3 cut(s) 488, 679, 1296
Bst4CI ACNGT 4 cut(s) 170, 338, 814, 1068
Bst6I CTCTTC 1 cut(s) 411
BstAPI GCANNNNNTGC 2 cut(s) 1624, 1629
BstAUI TGTACA 1 cut(s) 1549
BstC8I GCNNGC 3 cut(s) 58, 376, 1625
BstDEI CTNAG 7 cut(s) 10, 150, 459, 1354, 1509, 1585, 1604
BstDSI CCRYGG 2 cut(s) 738, 837
BstF5I GGATG 1 cut(s) 1019
BstFNI CGCG 3 cut(s) 344, 748, 1223
BstH2I RGCGCY 1 cut(s) 1503
BstHHI GCGC 4 cut(s) 346, 834, 1225, 1502
BstMAI GTCTC 4 cut(s) 146, 672, 1236, 1355
BstMWI GCNNNNNNNGC 8 cut(s) 11, 868, 1123, 1172, 1415, 1438, 1624, 1629
BstNI CCWGG 3 cut(s) 488, 679, 1296
BstNSI RCATGY 3 cut(s) 439, 1279, 1627
BstSCI CCNGG 5 cut(s) 244, 486, 677, 1294, 1568
BstSFI CTRYAG 2 cut(s) 1283, 1407
BstUI CGCG 3 cut(s) 344, 748, 1223
BstV1I GCAGC 4 cut(s) 83, 309, 1129, 1212
BstV2I GAAGAC 1 cut(s) 1314
BstX2I RGATCY 3 cut(s) 691, 1180, 1724
BstYI RGATCY 3 cut(s) 691, 1180, 1724
Bsu15I ATCGAT 2 cut(s) 423, 1578
BsuI GTATCC 1 cut(s) 532
BsuRI GGCC 3 cut(s) 737, 1117, 1514
BsuTUI ATCGAT 2 cut(s) 423, 1578
BtgI CCRYGG 2 cut(s) 738, 837
BtgZI GCGATG 1 cut(s) 1204
BtsCI GGATG 1 cut(s) 1019
BtsI GCAGTG 2 cut(s) 488, 1634
BtsIMutI CAGTG 4 cut(s) 488, 810, 1482, 1634
BtuMI TCGCGA 1 cut(s) 748
Cac8I GCNNGC 3 cut(s) 58, 376, 1625
CciI TCATGA 1 cut(s) 1745
CfoI GCGC 4 cut(s) 346, 834, 1225, 1502
Cfr13I GGNCC 2 cut(s) 707, 1116
ClaI ATCGAT 2 cut(s) 423, 1578
CsiI ACCWGGT 1 cut(s) 677
Csp6I GTAC 4 cut(s) 1289, 1370, 1480, 1550
CviQI GTAC 4 cut(s) 1289, 1370, 1480, 1550
DdeI CTNAG 7 cut(s) 10, 150, 459, 1354, 1509, 1585, 1604
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 3 cut(s) 560, 738, 837
Eco47I GGWCC 1 cut(s) 707
Eco57I CTGAAG 1 cut(s) 1326
Eco88I CYCGRG 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 1116
EcoRI GAATTC 2 cut(s) 406, 615
EcoRII CCWGG 3 cut(s) 486, 677, 1294
EcoT14I CCWWGG 3 cut(s) 560, 738, 837
ErhI CCWWGG 3 cut(s) 560, 738, 837
FalI AAGNNNNNCTT 2 cut(s) 544, 576
FaqI GGGAC 1 cut(s) 564
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 6 cut(s) 6, 97, 298, 342, 1143, 1226
FokI GGATG 1 cut(s) 1006
Fsp4HI GCNGC 6 cut(s) 6, 97, 298, 342, 1143, 1226
FspBI CTAG 4 cut(s) 521, 636, 698, 986
GlaI GCGC 4 cut(s) 345, 833, 1224, 1501
GluI GCNGC 6 cut(s) 6, 97, 298, 342, 1143, 1226
HaeII RGCGCY 1 cut(s) 1503
HaeIII GGCC 3 cut(s) 737, 1117, 1514
HapII CCGG 3 cut(s) 246, 1229, 1569
HhaI GCGC 4 cut(s) 346, 834, 1225, 1502
Hin6I GCGC 4 cut(s) 344, 832, 1223, 1500
HinP1I GCGC 4 cut(s) 344, 832, 1223, 1500
HindIII AAGCTT 1 cut(s) 1447
HinfI GANTC 7 cut(s) 146, 517, 577, 910, 1232, 1244, 1487
HpaII CCGG 3 cut(s) 246, 1229, 1569
Hpy166II GTNNAC 5 cut(s) 980, 1291, 1372, 1482, 1689
Hpy188I TCNGA 8 cut(s) 151, 460, 823, 879, 1306, 1510, 1588, 1607
Hpy188III TCNNGA 8 cut(s) 213, 428, 602, 612, 669, 747, 1072, 1746
Hpy8I GTNNAC 5 cut(s) 980, 1291, 1372, 1482, 1689
HpyAV CCTTC 3 cut(s) 324, 597, 1206
HpyCH4III ACNGT 4 cut(s) 170, 338, 814, 1068
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 868, 1123, 1172, 1415, 1438, 1624, 1629
HpyF3I CTNAG 7 cut(s) 10, 150, 459, 1354, 1509, 1585, 1604
HspAI GCGC 4 cut(s) 344, 832, 1223, 1500
Ksp22I TGATCA 1 cut(s) 874
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 4 cut(s) 83, 309, 1129, 1212
LweI GCATC 2 cut(s) 849, 1531
MabI ACCWGGT 1 cut(s) 677
MaeI CTAG 4 cut(s) 521, 636, 698, 986
MaeIII GTNAC 1 cut(s) 390
MboII GAAGA 6 cut(s) 118, 428, 686, 905, 1319, 1333
MflI RGATCY 3 cut(s) 691, 1180, 1724
MlyI GAGTC 5 cut(s) 140, 526, 586, 1238, 1481
MmeI TCCRAC 2 cut(s) 342, 942
MseI TTAA 5 cut(s) 41, 222, 938, 1130, 1529
MslI CAYNNNNRTG 3 cut(s) 356, 857, 1274
MspI CCGG 3 cut(s) 246, 1229, 1569
MspR9I CCNGG 5 cut(s) 246, 488, 679, 1296, 1570
MvaI CCWGG 3 cut(s) 488, 679, 1296
MvnI CGCG 3 cut(s) 344, 748, 1223
MwoI GCNNNNNNNGC 8 cut(s) 11, 868, 1123, 1172, 1415, 1438, 1624, 1629
NciI CCSGG 2 cut(s) 246, 1570
NcoI CCATGG 2 cut(s) 738, 837
NlaIV GGNNCC 3 cut(s) 779, 1099, 1118
NruI TCGCGA 1 cut(s) 748
NspI RCATGY 3 cut(s) 439, 1279, 1627
PaeI GCATGC 1 cut(s) 1627
PaeR7I CTCGAG 1 cut(s) 669
PagI TCATGA 1 cut(s) 1745
PciI ACATGT 1 cut(s) 1275
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 2 cut(s) 910, 1232
PflMI CCANNNNNTGG 1 cut(s) 447
PfoI TCCNGGA 1 cut(s) 244
PkrI GCNGC 6 cut(s) 7, 98, 299, 343, 1144, 1227
PleI GAGTC 5 cut(s) 140, 525, 585, 1238, 1481
PpsI GAGTC 5 cut(s) 140, 525, 585, 1238, 1481
PscI ACATGT 1 cut(s) 1275
Psp6I CCWGG 3 cut(s) 486, 677, 1294
PspGI CCWGG 3 cut(s) 486, 677, 1294
PspN4I GGNNCC 3 cut(s) 779, 1099, 1118
PspPI GGNCC 2 cut(s) 707, 1116
PstI CTGCAG 1 cut(s) 1411
PsuI RGATCY 3 cut(s) 691, 1180, 1724
RruI TCGCGA 1 cut(s) 748
RsaI GTAC 4 cut(s) 1290, 1371, 1481, 1551
RsaNI GTAC 4 cut(s) 1289, 1370, 1480, 1550
RseI CAYNNNNRTG 3 cut(s) 356, 857, 1274
SaqAI TTAA 5 cut(s) 41, 222, 938, 1130, 1529
SatI GCNGC 6 cut(s) 6, 97, 298, 342, 1143, 1226
Sau96I GGNCC 2 cut(s) 707, 1116
SchI GAGTC 5 cut(s) 140, 526, 586, 1238, 1481
ScrFI CCNGG 5 cut(s) 246, 488, 679, 1296, 1570
SexAI ACCWGGT 1 cut(s) 677
SfaNI GCATC 2 cut(s) 849, 1531
SfcI CTRYAG 2 cut(s) 1283, 1407
Sfr274I CTCGAG 1 cut(s) 669
SinI GGWCC 1 cut(s) 707
SlaI CTCGAG 1 cut(s) 669
SmiMI CAYNNNNRTG 3 cut(s) 356, 857, 1274
SmlI CTYRAG 2 cut(s) 211, 669
SmoI CTYRAG 2 cut(s) 211, 669
SphI GCATGC 1 cut(s) 1627
SsiI CCGC 2 cut(s) 342, 401
SspMI CTAG 4 cut(s) 521, 636, 698, 986
StyD4I CCNGG 5 cut(s) 244, 486, 677, 1294, 1568
StyI CCWWGG 3 cut(s) 560, 738, 837
TaaI ACNGT 4 cut(s) 170, 338, 814, 1068
TatI WGTACW 1 cut(s) 1549
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 910, 1232
Tru1I TTAA 5 cut(s) 41, 222, 938, 1130, 1529
Tru9I TTAA 5 cut(s) 41, 222, 938, 1130, 1529
TscAI CASTG 4 cut(s) 488, 817, 1489, 1634
TseI GCWGC 5 cut(s) 5, 96, 297, 1142, 1225
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 4 cut(s) 488, 817, 1489, 1634
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 707
XapI RAATTY 5 cut(s) 406, 508, 615, 712, 928
XceI RCATGY 3 cut(s) 439, 1279, 1627
XcmI CCANNNNNNNNNTGG 2 cut(s) 835, 844
XhoI CTCGAG 1 cut(s) 669
XspI CTAG 4 cut(s) 521, 636, 698, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.