Rh2CG533300

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004787
Physical Location & Seq
Forward (+)
70927786 .. 70966092
38307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG533300.1

Sequence Viewer

Length: 1257 bp
ATGGAGGTTTGTTGTGATCTTGAAGTGGATGTTAATGGTGAGGAGTCTTTCATGGTGAACAAGAGAGTTATTGCTTCCTATTCGGGCAAGTTGAGCAAGTTACTTGGCAAATCAAAAGGTTCCACAAGAAATCTTAAAGTTGTCTTCCATGACTTTCCAGGAAGGGCAGAGAGTTTTGAGCTCATTTCAAGGTTCTGCTACAACAATGGCAGCATTGACATAACTCCTTCCAATATTTCCCTCCTACATTTTGCTGCCATTTTCATGGAAATGAACATTTCTGTTTCCGGAGCACGCAATTTGTTTGAAAAAACCGAGAAATCACTTGAAGAGATGAGGTATTGGACTTGGTCTGAGCTCTTGATTGCTTTGAAGCAGTGCCAAGATTTACTTCCGGCAACTAATTGTTTGCCTCTACTTGAGAAATGCTTGGATTGCATTGTGGGAAGACTGGCTTTGGCCAGTGAAGCAAGTCCATGTCCTTCAACTTCATCCCCAGATAGCTCTGGCATTCGGTTTTCGTGTGACACTAGAAGCACCGAGAGCTTGAAAACCAGCTCCTCTCGCTCTACATGGTGGTTCGAGGATCTTCTAGTTTTAGGTCCCAATTTGGTTGAAATGCTGGTGAAGTCCATGGTTACTCGAAAGTTTGATCATGTTACTATCAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTCTACACTGTCGAATCCGATGCCAAGCGCACTATTGCAGAAGCAGTCATTGATATGCTTTACATTCTTGATCAGAGCTCTGTTTCTTGCAAAACTTTGTTTTGGATTCTTCGTGTTGTTCAGAATATAAACATAAACAAAAGCAGCAGGAATAAGTTGGAGAGCATGATTGGCTCACAGTTGGATCAAGCATCATTGGATAACTTGCTTGTTCCTTCCCCACATGGGATCAATCACTTGTATGATGTCAATCTTGTTTTGAGGTTTTTGAACTCATTTCTGCGCGGAGGAACAGCAAAAGCGTCTCCTATGAGATTGAGGAAAGTTGGAGTCTTGGTGGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTAAGGCCTTCAAAGTTTCTGGCTTTAGCCATGGTCCTGCCAGATGCTGCTAGAGACTCCTATGATGAACTTTACCGTGCCATCGATATGTATCTAGAGGTATTGCATTTATCTCAATTTTTTTTTTATTCAAGTCATCTAGTTTCAATTACCTATAATTTTAGCCGTGTACATAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

418

Amino Acids

47.31

Weight (kDa)

7.52

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 191 - 391 3e-50 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 984
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 1 cut(s) 984
AclWI GGATC 4 cut(s) 594, 891, 935, 1061
AcoI YGGCCR 1 cut(s) 459
AfaI GTAC 1 cut(s) 1245
AfiI CCNNNNNNNGG 1 cut(s) 924
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AluBI AGCT 6 cut(s) 181, 358, 504, 546, 558, 777
AluI AGCT 6 cut(s) 181, 358, 504, 546, 558, 777
Alw21I GWGCWC 4 cut(s) 183, 295, 360, 779
Alw26I GTCTC 2 cut(s) 1008, 1122
AlwI GGATC 4 cut(s) 594, 891, 935, 1061
AlwNI CAGNNNCTG 1 cut(s) 1121
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 2 cut(s) 459, 1079
ApeKI GCWGC 4 cut(s) 210, 254, 843, 1121
AspLEI GCGC 2 cut(s) 729, 984
AspS9I GGNCC 2 cut(s) 602, 1108
AsuHPI GGTGA 3 cut(s) 50, 67, 637
AvaII GGWCC 2 cut(s) 602, 1108
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 3 cut(s) 183, 360, 779
BbsI GAAGAC 2 cut(s) 136, 454
Bbv12I GWGCWC 4 cut(s) 183, 295, 360, 779
BbvI GCAGC 4 cut(s) 222, 241, 855, 1108
BccI CCATC 1 cut(s) 1163
BceAI ACGGC 1 cut(s) 1224
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 2 cut(s) 652, 769
BcoDI GTCTC 2 cut(s) 1008, 1122
BfaI CTAG 5 cut(s) 531, 593, 1125, 1169, 1214
BisI GCNGC 4 cut(s) 211, 255, 844, 1122
BlsI GCNGC 4 cut(s) 212, 256, 845, 1123
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 2 cut(s) 602, 1108
BmgT120I GGNCC 2 cut(s) 602, 1108
BmiI GGNNCC 3 cut(s) 121, 604, 674
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 709, 899, 1108
BpiI GAAGAC 2 cut(s) 136, 454
BpuEI CTTGAG 1 cut(s) 440
Bsa29I ATCGAT 1 cut(s) 1158
BsaBI GATNNNNATC 2 cut(s) 948, 1164
BsaJI CCNNGG 2 cut(s) 633, 1104
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 924
Bse1I ACTGG 2 cut(s) 456, 462
Bse8I GATNNNNATC 2 cut(s) 948, 1164
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 1158
BseDI CCNNGG 2 cut(s) 633, 1104
BseGI GGATG 2 cut(s) 34, 491
BseJI GATNNNNATC 2 cut(s) 948, 1164
BseLI CCNNNNNNNGG 1 cut(s) 924
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 456, 462
BseRI GAGGAG 2 cut(s) 56, 550
BseXI GCAGC 4 cut(s) 222, 241, 855, 1108
Bsh1236I CGCG 1 cut(s) 984
BshFI GGCC 2 cut(s) 461, 1081
BshVI ATCGAT 1 cut(s) 1158
BsiHKAI GWGCWC 4 cut(s) 183, 295, 360, 779
BsiSI CCGG 2 cut(s) 288, 395
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 1 cut(s) 924
BsmAI GTCTC 2 cut(s) 1008, 1122
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 2 cut(s) 461, 1081
Bsp1286I GDGCHC 4 cut(s) 183, 295, 360, 779
Bsp13I TCCGGA 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 1243
Bsp143I GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
Bsp19I CCATGG 2 cut(s) 633, 1104
BspACI CCGC 1 cut(s) 984
BspANI GGCC 2 cut(s) 461, 1081
BspCNI CTCAG 1 cut(s) 346
BspDI ATCGAT 1 cut(s) 1158
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 984
BspLI GGNNCC 3 cut(s) 121, 604, 674
BspPI GGATC 4 cut(s) 594, 891, 935, 1061
BsrGI TGTACA 1 cut(s) 1243
BsrI ACTGG 2 cut(s) 456, 462
BssECI CCNNGG 2 cut(s) 633, 1104
BssMI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
BssT1I CCWWGG 2 cut(s) 633, 1104
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 3 cut(s) 709, 879, 1151
Bst6I CTCTTC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 1243
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 2 cut(s) 633, 1104
BstF5I GGATG 2 cut(s) 34, 491
BstFNI CGCG 1 cut(s) 984
BstHHI GCGC 2 cut(s) 729, 984
BstKTI GATC 7 cut(s) 19, 589, 655, 772, 886, 930, 1069
BstMAI GTCTC 2 cut(s) 1008, 1122
BstMBI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
BstMWI GCNNNNNNNGC 7 cut(s) 93, 435, 467, 543, 564, 870, 1058
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstUI CGCG 1 cut(s) 984
BstV1I GCAGC 4 cut(s) 222, 241, 855, 1108
BstV2I GAAGAC 2 cut(s) 136, 454
BstX2I RGATCY 2 cut(s) 586, 1066
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 2 cut(s) 586, 1066
Bsu15I ATCGAT 1 cut(s) 1158
BsuRI GGCC 2 cut(s) 461, 1081
BsuTUI ATCGAT 1 cut(s) 1158
BtgI CCRYGG 2 cut(s) 633, 1104
BtsCI GGATG 2 cut(s) 34, 491
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 3 cut(s) 383, 469, 705
Cac8I GCNNGC 1 cut(s) 295
CaiI CAGNNNCTG 1 cut(s) 1121
CfoI GCGC 2 cut(s) 729, 984
Cfr13I GGNCC 2 cut(s) 602, 1108
ClaI ATCGAT 1 cut(s) 1158
CseI GACGC 1 cut(s) 990
Csp6I GTAC 1 cut(s) 1244
CviQI GTAC 1 cut(s) 1244
DdeI CTNAG 1 cut(s) 354
DpnI GATC 7 cut(s) 18, 588, 654, 771, 885, 929, 1068
DpnII GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 3 cut(s) 181, 358, 777
Eco130I CCWWGG 2 cut(s) 633, 1104
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 3 cut(s) 183, 360, 779
Eco47I GGWCC 2 cut(s) 602, 1108
Eco53kI GAGCTC 3 cut(s) 181, 358, 777
EcoICRI GAGCTC 3 cut(s) 181, 358, 777
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 633, 1104
EcoT38I GRGCYC 3 cut(s) 183, 360, 779
ErhI CCWWGG 2 cut(s) 633, 1104
Esp3I CGTCTC 1 cut(s) 1008
FalI AAGNNNNNCTT 4 cut(s) 375, 407, 439, 471
FaqI GGGAC 1 cut(s) 588
FbaI TGATCA 2 cut(s) 652, 769
Fnu4HI GCNGC 4 cut(s) 211, 255, 844, 1122
FokI GGATG 2 cut(s) 41, 478
FriOI GRGCYC 3 cut(s) 183, 360, 779
Fsp4HI GCNGC 4 cut(s) 211, 255, 844, 1122
FspBI CTAG 5 cut(s) 531, 593, 1125, 1169, 1214
GlaI GCGC 2 cut(s) 728, 983
GluI GCNGC 4 cut(s) 211, 255, 844, 1122
HaeIII GGCC 2 cut(s) 461, 1081
HapII CCGG 2 cut(s) 288, 395
HgaI GACGC 1 cut(s) 990
HhaI GCGC 2 cut(s) 729, 984
Hin6I GCGC 2 cut(s) 727, 982
HinP1I GCGC 2 cut(s) 727, 982
HinfI GANTC 5 cut(s) 44, 713, 805, 1029, 1130
HpaII CCGG 2 cut(s) 288, 395
HphI GGTGA 3 cut(s) 50, 67, 637
Hpy166II GTNNAC 2 cut(s) 58, 1244
Hpy188I TCNGA 4 cut(s) 355, 718, 774, 822
Hpy188III TCNNGA 5 cut(s) 20, 288, 361, 767, 1169
Hpy8I GTNNAC 2 cut(s) 58, 1244
HpyAV CCTTC 5 cut(s) 156, 237, 492, 924, 1092
HpyCH4III ACNGT 3 cut(s) 709, 879, 1151
HpyCH4V TGCA 4 cut(s) 438, 737, 789, 1180
HpyF10VI GCNNNNNNNGC 7 cut(s) 93, 435, 467, 543, 564, 870, 1058
HpyF3I CTNAG 1 cut(s) 354
HspAI GCGC 2 cut(s) 727, 982
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 652, 769
Kzo9I GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
LmnI GCTCC 2 cut(s) 290, 563
Lsp1109I GCAGC 4 cut(s) 222, 241, 855, 1108
LweI GCATC 3 cut(s) 709, 899, 1108
MaeI CTAG 5 cut(s) 531, 593, 1125, 1169, 1214
MaeIII GTNAC 4 cut(s) 99, 524, 637, 658
MalI GATC 7 cut(s) 18, 588, 654, 771, 885, 929, 1068
MboI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
MboII GAAGA 5 cut(s) 136, 341, 459, 581, 800
MflI RGATCY 2 cut(s) 586, 1066
MhlI GDGCHC 4 cut(s) 183, 295, 360, 779
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 6 cut(s) 298, 403, 607, 1190, 1221, 1231
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 3 cut(s) 53, 1038, 1124
MmeI TCCRAC 3 cut(s) 837, 861, 1006
Mox20I TGGCCA 1 cut(s) 461
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 461
MseI TTAA 3 cut(s) 33, 135, 1076
MslI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 2 cut(s) 288, 395
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 1 cut(s) 984
MwoI GCNNNNNNNGC 7 cut(s) 93, 435, 467, 543, 564, 870, 1058
NcoI CCATGG 2 cut(s) 633, 1104
NdeII GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
NlaIV GGNNCC 3 cut(s) 121, 604, 674
NmuCI GTSAC 1 cut(s) 524
PceI AGGCCT 1 cut(s) 1081
PctI GAATGC 1 cut(s) 510
PfeI GAWTC 2 cut(s) 713, 805
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 4 cut(s) 212, 256, 845, 1123
PleI GAGTC 3 cut(s) 52, 1037, 1124
PpsI GAGTC 3 cut(s) 52, 1037, 1124
PpuMI RGGWCCY 1 cut(s) 602
Psp124BI GAGCTC 3 cut(s) 183, 360, 779
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 121, 604, 674
PspPI GGNCC 2 cut(s) 602, 1108
PspPPI RGGWCCY 1 cut(s) 602
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 2 cut(s) 586, 1066
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 1 cut(s) 1245
RsaNI GTAC 1 cut(s) 1244
RseI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
SacI GAGCTC 3 cut(s) 183, 360, 779
SaqAI TTAA 3 cut(s) 33, 135, 1076
SatI GCNGC 4 cut(s) 211, 255, 844, 1122
Sau3AI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
Sau96I GGNCC 2 cut(s) 602, 1108
SchI GAGTC 3 cut(s) 53, 1038, 1124
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 4 cut(s) 183, 295, 360, 779
SfaNI GCATC 3 cut(s) 709, 899, 1108
SinI GGWCC 2 cut(s) 602, 1108
SmiMI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 6 cut(s) 298, 403, 607, 1190, 1221, 1231
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 1 cut(s) 984
SspI AATATT 1 cut(s) 235
SspMI CTAG 5 cut(s) 531, 593, 1125, 1169, 1214
SstI GAGCTC 3 cut(s) 183, 360, 779
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 633, 1104
TaaI ACNGT 3 cut(s) 709, 879, 1151
TaqI TCGA 5 cut(s) 582, 643, 695, 711, 1158
TasI AATT 6 cut(s) 298, 403, 607, 1190, 1221, 1231
TatI WGTACW 1 cut(s) 1243
TfiI GAWTC 2 cut(s) 713, 805
Tru1I TTAA 3 cut(s) 33, 135, 1076
Tru9I TTAA 3 cut(s) 33, 135, 1076
TscAI CASTG 3 cut(s) 383, 469, 712
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 4 cut(s) 210, 254, 843, 1121
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 5 cut(s) 40, 253, 287, 480, 1155
TspRI CASTG 3 cut(s) 383, 469, 712
Tth111I GACNNNGTC 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 602, 1108
XbaI TCTAGA 1 cut(s) 1168
XspI CTAG 5 cut(s) 531, 593, 1125, 1169, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.