RLG00000021221

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004787
Physical Location & Seq
Forward (+)
73073007 .. 73075526
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021221

Sequence Viewer

Length: 1635 bp
ATGGAGGTTTGCTGTGATCTTGAAGTGGATGTTAATGGTGAGGAGTCTTTCATGGTGAACAAGAGAGTTATTGCTTCCTATTCAGGCAAGTTGAGCAAGTTACTTGGCAAATCAAAAGGTTCCACAAGAAATCTTAAAGTTGTCTTCCATGACTTTCCAGGAGGGGCAGAGAGTTTTGAGCTCATTTCAAGGTTCTGCTACAACAATGGCAGCATTGACATAACTCCTTCCAATATTTCCCTCCTACATTTTGCTGCCATTTTCATGGAAATGAACATTTCTGTTTCCGGAGCAGGCAATTTGTTCGAAAAAACCGAGAAATCACTTGAAGAGATGAGGTATTGGACTTGGTCTGAGCTCTTGATTGCTTTGAAGCAGTGCCAAGATTTACTTCCGGCAACTAATTGTTTGCCTCAACTTGAGAAATGCTTGGATTGCATTGTGGGAAGACTGGCTTTGGCCAGTGAAGCAAGTCCATGTCCTTCAACTTCATCCCCAGATAGCTCTGGCATTCGGTTTTCGTGTGACACTAGAAGCACCGAGAGCTTGAAAACCAGCTCCTCTCGCTCAACATGGTGGTTCGAGGATCTTCTAGTTTTAGGTCCCAATTTGGTTGAAATGCTGGTGAAATCCATGGTTACTCGAAAGTTTGATCATGTTGCTATCAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTCTACACTGTCGAATCCGATGCCAAGCGCACTATTGCAGAAGCAGTCATTGATATGCTTTACATTCTTGATCAGAGCTCTGTTTCTTGCAAAACTTTGTTTTGGATTCTTCGTGTTGTTCAGAATATAAACATATACAAAAGCAGCAGGAATAAGTTGGAGAGCATGATTGGTTCACATTTGGATCAAGCATCATTGGATAACTTGCTTGTTCCTTCCCCACATGGGATCAATCACTTGTATGATGTCAATCTTGTTTTGAGGTTTTTGAACTCATTTCTGCATGGAGGAACAGCAAAAGCGTCTCCTATGAGATTGAGGAAAGTTGGAGTCTTGGTGGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTAAGGCCTTCAAAGTTTCTGGCTTTAGCCATGGTCCTGCCAGATGCTGCGAGAGACTCCTATGATGAGCTTTACCGTGCCATCGATATGTATCTAGAGGTACATGGAGGATTGTCCGAAGAGGAAAAGATGAAACTCTGTTGTTTATTGAACTATGAGAAGCTCTCGGAAGAAGCTTGCATACACCTCTCTCAGAATGCAAAATTCCCATCAAAATCAGCAGTCCAAGCACTCATTTCTCAGCAAGTCAAGCTTAAGAGCTTATTTCATGCCACGAACAATCCCAAATCCCATGGTGACTCCCCTTTTAGTGTCACTCAGGTAGATAATGAGGGAAGGAATGGTCATGAATCCAATGAACAACGCGTGCTTTATGCTGGAAAACTTGATCTCTCGGCTGACAACGAGAAACTGAGGGAACATTTACATGGCATGCAGTGGAGGGTCATGGAGTTGGAAAAAATGTGCAAGAAAATGCAAACCCAAATGGCAAAGTTCACAAAATCAAGAGTGTTATCAAGCCACAGTCATGCAAGATCTTTGCCCAAACTTTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

545

Amino Acids

61.17

Weight (kDa)

7.53

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 191 - 418 2.4e-62 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1440
AccIII TCCGGA 1 cut(s) 287
AclWI GGATC 4 cut(s) 594, 891, 935, 1061
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 1 cut(s) 1277
AfaI GTAC 1 cut(s) 1176
AfiI CCNNNNNNNGG 1 cut(s) 924
AflII CTTAAG 1 cut(s) 1328
AflIII ACRYGT 1 cut(s) 1438
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
Alw21I GWGCWC 3 cut(s) 183, 360, 779
Alw26I GTCTC 2 cut(s) 1008, 1122
AlwI GGATC 4 cut(s) 594, 891, 935, 1061
AlwNI CAGNNNCTG 1 cut(s) 1121
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 2 cut(s) 459, 1079
ApeKI GCWGC 4 cut(s) 210, 254, 843, 1121
ApoI RAATTY 1 cut(s) 1277
AspLEI GCGC 1 cut(s) 729
AspS9I GGNCC 2 cut(s) 602, 1108
AsuHPI GGTGA 4 cut(s) 50, 67, 637, 1382
AsuII TTCGAA 1 cut(s) 306
AvaII GGWCC 2 cut(s) 602, 1108
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 3 cut(s) 183, 360, 779
BarI GAAGNNNNNNTAC 2 cut(s) 1239, 1271
BbsI GAAGAC 2 cut(s) 136, 454
Bbv12I GWGCWC 3 cut(s) 183, 360, 779
BbvI GCAGC 4 cut(s) 222, 241, 855, 1108
BccI CCATC 2 cut(s) 1163, 1291
BcgI CGANNNNNNTGC 4 cut(s) 286, 320, 701, 735
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 2 cut(s) 652, 769
BcoDI GTCTC 2 cut(s) 1008, 1122
BfaI CTAG 3 cut(s) 531, 593, 1169
BfrI CTTAAG 1 cut(s) 1328
BglII AGATCT 1 cut(s) 1610
BisI GCNGC 4 cut(s) 211, 255, 844, 1122
BlsI GCNGC 4 cut(s) 212, 256, 845, 1123
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 2 cut(s) 602, 1108
BmgT120I GGNCC 2 cut(s) 602, 1108
BmiI GGNNCC 3 cut(s) 121, 604, 674
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 709, 899, 1108
BpiI GAAGAC 2 cut(s) 136, 454
BplI GAGNNNNNCTC 2 cut(s) 1223, 1255
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 440
Bsa29I ATCGAT 1 cut(s) 1158
BsaBI GATNNNNATC 2 cut(s) 948, 1164
BsaJI CCNNGG 3 cut(s) 633, 1104, 1366
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 924
Bse1I ACTGG 2 cut(s) 456, 462
Bse8I GATNNNNATC 2 cut(s) 948, 1164
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 1158
BseDI CCNNGG 3 cut(s) 633, 1104, 1366
BseGI GGATG 2 cut(s) 34, 491
BseJI GATNNNNATC 2 cut(s) 948, 1164
BseLI CCNNNNNNNGG 1 cut(s) 924
BseMII CTCAG 5 cut(s) 345, 1280, 1328, 1406, 1478
BseNI ACTGG 2 cut(s) 456, 462
BseRI GAGGAG 2 cut(s) 56, 550
BseXI GCAGC 4 cut(s) 222, 241, 855, 1108
Bsh1236I CGCG 1 cut(s) 1440
BshFI GGCC 2 cut(s) 461, 1081
BshVI ATCGAT 1 cut(s) 1158
BsiHKAI GWGCWC 3 cut(s) 183, 360, 779
BsiSI CCGG 2 cut(s) 288, 395
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 1 cut(s) 924
BsmAI GTCTC 2 cut(s) 1008, 1122
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 2 cut(s) 510, 1276
BsnI GGCC 2 cut(s) 461, 1081
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 3 cut(s) 183, 360, 779
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
Bsp19I CCATGG 3 cut(s) 633, 1104, 1366
BspANI GGCC 2 cut(s) 461, 1081
BspCNI CTCAG 5 cut(s) 346, 1279, 1327, 1405, 1479
BspDI ATCGAT 1 cut(s) 1158
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 1440
BspHI TCATGA 2 cut(s) 1420, 1631
BspLI GGNNCC 3 cut(s) 121, 604, 674
BspPI GGATC 4 cut(s) 594, 891, 935, 1061
BspT104I TTCGAA 1 cut(s) 306
BspTI CTTAAG 1 cut(s) 1328
BsrI ACTGG 2 cut(s) 456, 462
BssECI CCNNGG 3 cut(s) 633, 1104, 1366
BssMI GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
BssT1I CCWWGG 3 cut(s) 633, 1104, 1366
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 3 cut(s) 709, 1151, 1601
Bst6I CTCTTC 2 cut(s) 324, 1188
BstAFI CTTAAG 1 cut(s) 1328
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 4 cut(s) 295, 1252, 1442, 1508
BstDEI CTNAG 5 cut(s) 354, 1266, 1314, 1392, 1487
BstDSI CCRYGG 3 cut(s) 633, 1104, 1366
BstF5I GGATG 2 cut(s) 34, 491
BstFNI CGCG 1 cut(s) 1440
BstHHI GCGC 1 cut(s) 729
BstKTI GATC 9 cut(s) 19, 589, 655, 772, 886, 930, 1069, 1465, 1613
BstMAI GTCTC 2 cut(s) 1008, 1122
BstMBI GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
BstMWI GCNNNNNNNGC 8 cut(s) 93, 435, 467, 543, 564, 1058, 1301, 1324
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 1510
BstSCI CCNGG 1 cut(s) 157
BstUI CGCG 1 cut(s) 1440
BstV1I GCAGC 4 cut(s) 222, 241, 855, 1108
BstV2I GAAGAC 2 cut(s) 136, 454
BstX2I RGATCY 3 cut(s) 586, 1066, 1610
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 3 cut(s) 586, 1066, 1610
Bsu15I ATCGAT 1 cut(s) 1158
BsuRI GGCC 2 cut(s) 461, 1081
BsuTUI ATCGAT 1 cut(s) 1158
BtgI CCRYGG 3 cut(s) 633, 1104, 1366
BtsCI GGATG 2 cut(s) 34, 491
BtsI GCAGTG 2 cut(s) 383, 1517
BtsIMutI CAGTG 4 cut(s) 383, 469, 705, 1517
Cac8I GCNNGC 4 cut(s) 295, 1252, 1442, 1508
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 2 cut(s) 1420, 1631
CfoI GCGC 1 cut(s) 729
Cfr13I GGNCC 2 cut(s) 602, 1108
ClaI ATCGAT 1 cut(s) 1158
CseI GACGC 1 cut(s) 990
Csp6I GTAC 1 cut(s) 1175
CviQI GTAC 1 cut(s) 1175
DdeI CTNAG 5 cut(s) 354, 1266, 1314, 1392, 1487
DpnI GATC 9 cut(s) 18, 588, 654, 771, 885, 929, 1068, 1464, 1612
DpnII GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 324, 1188
EarI CTCTTC 2 cut(s) 324, 1188
Ecl136II GAGCTC 3 cut(s) 181, 358, 777
Eco130I CCWWGG 3 cut(s) 633, 1104, 1366
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 3 cut(s) 183, 360, 779
Eco47I GGWCC 2 cut(s) 602, 1108
Eco53kI GAGCTC 3 cut(s) 181, 358, 777
EcoICRI GAGCTC 3 cut(s) 181, 358, 777
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 3 cut(s) 633, 1104, 1366
EcoT38I GRGCYC 3 cut(s) 183, 360, 779
ErhI CCWWGG 3 cut(s) 633, 1104, 1366
Esp3I CGTCTC 1 cut(s) 1008
FalI AAGNNNNNCTT 6 cut(s) 375, 407, 439, 471, 1311, 1343
FaqI GGGAC 1 cut(s) 588
FbaI TGATCA 2 cut(s) 652, 769
Fnu4HI GCNGC 4 cut(s) 211, 255, 844, 1122
FokI GGATG 2 cut(s) 41, 478
FriOI GRGCYC 3 cut(s) 183, 360, 779
Fsp4HI GCNGC 4 cut(s) 211, 255, 844, 1122
FspBI CTAG 3 cut(s) 531, 593, 1169
GlaI GCGC 1 cut(s) 728
GluI GCNGC 4 cut(s) 211, 255, 844, 1122
HaeIII GGCC 2 cut(s) 461, 1081
HapII CCGG 2 cut(s) 288, 395
HgaI GACGC 1 cut(s) 990
HhaI GCGC 1 cut(s) 729
Hin6I GCGC 1 cut(s) 727
HinP1I GCGC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 1248, 1325
HinfI GANTC 7 cut(s) 44, 713, 805, 1029, 1130, 1373, 1424
HpaII CCGG 2 cut(s) 288, 395
HphI GGTGA 4 cut(s) 50, 67, 637, 1382
Hpy166II GTNNAC 3 cut(s) 58, 875, 1572
Hpy188I TCNGA 7 cut(s) 355, 718, 774, 822, 1192, 1243, 1269
Hpy188III TCNNGA 8 cut(s) 20, 288, 361, 767, 1169, 1421, 1581, 1632
Hpy8I GTNNAC 3 cut(s) 58, 875, 1572
HpyAV CCTTC 5 cut(s) 237, 492, 924, 1092, 1404
HpyCH4III ACNGT 3 cut(s) 709, 1151, 1601
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 435, 467, 543, 564, 1058, 1301, 1324
HpyF3I CTNAG 5 cut(s) 354, 1266, 1314, 1392, 1487
HspAI GCGC 1 cut(s) 727
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 652, 769
Kzo9I GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
LmnI GCTCC 2 cut(s) 290, 563
Lsp1109I GCAGC 4 cut(s) 222, 241, 855, 1108
LweI GCATC 3 cut(s) 709, 899, 1108
MaeI CTAG 3 cut(s) 531, 593, 1169
MaeIII GTNAC 5 cut(s) 99, 524, 637, 1370, 1387
MalI GATC 9 cut(s) 18, 588, 654, 771, 885, 929, 1068, 1464, 1612
MboI GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
MboII GAAGA 7 cut(s) 136, 341, 459, 581, 800, 1205, 1256
MflI RGATCY 3 cut(s) 586, 1066, 1610
MhlI GDGCHC 3 cut(s) 183, 360, 779
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 4 cut(s) 298, 403, 607, 1277
MluI ACGCGT 1 cut(s) 1438
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 4 cut(s) 53, 1038, 1124, 1367
MmeI TCCRAC 3 cut(s) 837, 1006, 1509
Mox20I TGGCCA 1 cut(s) 461
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 461
MseI TTAA 4 cut(s) 33, 135, 1076, 1329
MslI CAYNNNNRTG 7 cut(s) 263, 269, 752, 939, 1160, 1500, 1602
Msp20I TGGCCA 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 1328
MspI CCGG 2 cut(s) 288, 395
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 2 cut(s) 510, 1276
MvaI CCWGG 1 cut(s) 159
MvnI CGCG 1 cut(s) 1440
MwoI GCNNNNNNNGC 8 cut(s) 93, 435, 467, 543, 564, 1058, 1301, 1324
NcoI CCATGG 3 cut(s) 633, 1104, 1366
NdeII GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
NlaIV GGNNCC 3 cut(s) 121, 604, 674
NmeAIII GCCGAG 1 cut(s) 1448
NmuCI GTSAC 3 cut(s) 524, 1370, 1387
NspI RCATGY 1 cut(s) 1510
NspV TTCGAA 1 cut(s) 306
PaeI GCATGC 1 cut(s) 1510
PagI TCATGA 2 cut(s) 1420, 1631
PceI AGGCCT 1 cut(s) 1081
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 510, 1276
PfeI GAWTC 3 cut(s) 713, 805, 1424
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 4 cut(s) 212, 256, 845, 1123
PleI GAGTC 4 cut(s) 52, 1037, 1124, 1367
PpsI GAGTC 4 cut(s) 52, 1037, 1124, 1367
PpuMI RGGWCCY 1 cut(s) 602
Psp124BI GAGCTC 3 cut(s) 183, 360, 779
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 3 cut(s) 121, 604, 674
PspPI GGNCC 2 cut(s) 602, 1108
PspPPI RGGWCCY 1 cut(s) 602
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 3 cut(s) 586, 1066, 1610
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 1 cut(s) 1176
RsaNI GTAC 1 cut(s) 1175
RseI CAYNNNNRTG 7 cut(s) 263, 269, 752, 939, 1160, 1500, 1602
SacI GAGCTC 3 cut(s) 183, 360, 779
SaqAI TTAA 4 cut(s) 33, 135, 1076, 1329
SatI GCNGC 4 cut(s) 211, 255, 844, 1122
Sau3AI GATC 9 cut(s) 16, 586, 652, 769, 883, 927, 1066, 1462, 1610
Sau96I GGNCC 2 cut(s) 602, 1108
SchI GAGTC 4 cut(s) 53, 1038, 1124, 1367
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 3 cut(s) 183, 360, 779
SfaNI GCATC 3 cut(s) 709, 899, 1108
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 2 cut(s) 602, 1108
SmiMI CAYNNNNRTG 7 cut(s) 263, 269, 752, 939, 1160, 1500, 1602
SmlI CTYRAG 2 cut(s) 419, 1328
SmoI CTYRAG 2 cut(s) 419, 1328
SphI GCATGC 1 cut(s) 1510
Sse9I AATT 4 cut(s) 298, 403, 607, 1277
SseBI AGGCCT 1 cut(s) 1081
SspI AATATT 1 cut(s) 235
SspMI CTAG 3 cut(s) 531, 593, 1169
SstI GAGCTC 3 cut(s) 183, 360, 779
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 3 cut(s) 633, 1104, 1366
TaaI ACNGT 3 cut(s) 709, 1151, 1601
TaqI TCGA 6 cut(s) 306, 582, 643, 695, 711, 1158
TasI AATT 4 cut(s) 298, 403, 607, 1277
TfiI GAWTC 3 cut(s) 713, 805, 1424
Tru1I TTAA 4 cut(s) 33, 135, 1076, 1329
Tru9I TTAA 4 cut(s) 33, 135, 1076, 1329
TscAI CASTG 4 cut(s) 383, 469, 712, 1517
TseFI GTSAC 3 cut(s) 524, 1370, 1387
TseI GCWGC 4 cut(s) 210, 254, 843, 1121
Tsp45I GTSAC 3 cut(s) 524, 1370, 1387
TspDTI ATGAA 9 cut(s) 40, 253, 287, 480, 1220, 1331, 1437, 1446, 1620
TspRI CASTG 4 cut(s) 383, 469, 712, 1517
Tth111I GACNNNGTC 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 1328
VpaK11BI GGWCC 2 cut(s) 602, 1108
XapI RAATTY 1 cut(s) 1277
XbaI TCTAGA 1 cut(s) 1168
XceI RCATGY 1 cut(s) 1510
XspI CTAG 3 cut(s) 531, 593, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.