Rh2DG572000

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004787
Physical Location & Seq
Forward (+)
80206811 .. 80216038
9228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG572000.1

Sequence Viewer

Length: 1266 bp
ATGGAGGTTTGCTGTGATCTTGAAGTGGATGTTAATGGTGAGGAGTCTTTCATGGTGAACAAGAGAGTTATTGCTTCCTATTCGGGCAAGTTGAGCAAGTTACTTGGCAAATCAAAAGGTTCCACAAGAAATCTTAAAGTTGTCTTCCATGACTTTCCAGGAGGGGCAGAGAGTTTTGAGCTCATTTCAAGGTTCTGCTACAACAATGGCAGCATTGACATAACTCCTTCCAATATTTCCCTCCTACATTTTGCAGCCATTTTCATGGAAATGAACATTTCTGTTTCCGGAGCACGCAATTTGTTCGAAAAAACTGAGAAATCACTTGAAGAGATGAGGTATTGGACATGGTCTGAGCTCTTGATTGCTTTGAAGCAGTGCCAAGATTTGCTTCCGGCGACTAATTGTTTGCCTCTACTTGAGAAATGCTTGGATTGCATTGTGGGAAGACTGGCTTTGGCCAGTGAAGCAAGCCCATGTCCTTCAACTTCATCCCCAGATAGCTCTGGCATTCGGTTTTCGTGTGACACTAGAAGCACCGAGAGCTTGAAAACCAGCTCCTCTCGCTCAACATGGTGGTTTGAGGATCTTCTAGTTTTGGGTCCCAATTTGGTTGAAATGCTGGTGAAATCCATGGTTACTCGAAAGTTTGATCATGTTATTATCAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTCTACACCGTCGAATCCGATGCCAAGTGCACTATTGCAGCAGCAGTCATTGATATGCTTTACATTCTTGATCAGAGCTCTGTTTCTTGCAAAACTTTGTTTTGGATTCTTCGTGTTGTTCAGAATATGAACATAAACAAAAGCAGCAGGAATAAGTTGGAGAGCATGATTGGTTCACAGTTGGATCAAGCATCATTAGATAACTTGCTTGTTCCTTCCCCACATGGGATCAATCACTTGTATGATGTCAATCTTGTTTTGAGGTTTTTGAACTCATTTCTGCGTGGAGGAACAGAAAAAGCGTCTCCTATGAGATTGAGGAAAGTTGGAGTCTTGGTGGATTTGTATATAGCAGAAGTAGCCCCAGATCCTTGTTTAAGGCCTTCAAAGTTTCTAGCTTTAGCCATGGTCCTGCCAGATGCTGCCAGAGACTCATATGATGAGCTTTACCGTGCCATCGATATGTATCTAGAGTTGGGCAAATCTACCCAACAAAATCTTCATATACAACAAACTGTTCCATTTTATGAACAGAGAAAGCACTCAAAAGGCTTCCATATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

421

Amino Acids

47.45

Weight (kDa)

7.05

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 191 - 391 5.8e-49 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 287
AclWI GGATC 4 cut(s) 594, 891, 935, 1061
AcoI YGGCCR 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 924
AgsI TTSAA 9 cut(s) 23, 189, 329, 373, 486, 550, 617, 970, 1086
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 4 cut(s) 375, 407, 1182, 1214
AluBI AGCT 8 cut(s) 181, 358, 504, 546, 558, 777, 1097, 1144
AluI AGCT 8 cut(s) 181, 358, 504, 546, 558, 777, 1097, 1144
Alw21I GWGCWC 5 cut(s) 183, 295, 360, 731, 779
Alw26I GTCTC 2 cut(s) 1008, 1122
Alw44I GTGCAC 1 cut(s) 727
AlwI GGATC 4 cut(s) 594, 891, 935, 1061
AlwNI CAGNNNCTG 1 cut(s) 1121
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 2 cut(s) 459, 1079
ApaLI GTGCAC 1 cut(s) 727
ApeKI GCWGC 6 cut(s) 210, 254, 737, 740, 843, 1121
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AspS9I GGNCC 2 cut(s) 602, 1108
AsuHPI GGTGA 3 cut(s) 50, 67, 637
AsuII TTCGAA 1 cut(s) 306
AvaII GGWCC 2 cut(s) 602, 1108
BaeGI GKGCMC 1 cut(s) 731
BalI TGGCCA 1 cut(s) 461
BanII GRGCYC 3 cut(s) 183, 360, 779
BbsI GAAGAC 2 cut(s) 136, 454
Bbv12I GWGCWC 5 cut(s) 183, 295, 360, 731, 779
BbvI GCAGC 6 cut(s) 222, 266, 749, 752, 855, 1108
BccI CCATC 1 cut(s) 1163
BcgI CGANNNNNNTGC 4 cut(s) 286, 320, 701, 735
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 2 cut(s) 652, 769
BcoDI GTCTC 2 cut(s) 1008, 1122
BfaI CTAG 4 cut(s) 531, 593, 1094, 1169
BisI GCNGC 6 cut(s) 211, 255, 738, 741, 844, 1122
BlsI GCNGC 6 cut(s) 212, 256, 739, 742, 845, 1123
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 2 cut(s) 602, 1108
BmgT120I GGNCC 2 cut(s) 602, 1108
BmiI GGNNCC 4 cut(s) 121, 603, 604, 674
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 3 cut(s) 709, 899, 1108
BpiI GAAGAC 2 cut(s) 136, 454
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 440
Bsa29I ATCGAT 1 cut(s) 1158
BsaBI GATNNNNATC 2 cut(s) 948, 1164
BsaJI CCNNGG 2 cut(s) 633, 1104
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 924
Bse1I ACTGG 2 cut(s) 456, 462
Bse8I GATNNNNATC 2 cut(s) 948, 1164
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 1158
BseDI CCNNGG 2 cut(s) 633, 1104
BseGI GGATG 2 cut(s) 34, 491
BseJI GATNNNNATC 2 cut(s) 948, 1164
BseLI CCNNNNNNNGG 1 cut(s) 924
BseMII CTCAG 2 cut(s) 306, 345
BseNI ACTGG 2 cut(s) 456, 462
BseRI GAGGAG 2 cut(s) 56, 550
BseSI GKGCMC 1 cut(s) 731
BseXI GCAGC 6 cut(s) 222, 266, 749, 752, 855, 1108
BshFI GGCC 2 cut(s) 461, 1081
BshVI ATCGAT 1 cut(s) 1158
BsiHKAI GWGCWC 5 cut(s) 183, 295, 360, 731, 779
BsiSI CCGG 2 cut(s) 288, 395
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 1 cut(s) 924
BsmAI GTCTC 2 cut(s) 1008, 1122
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 2 cut(s) 461, 1081
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 5 cut(s) 183, 295, 360, 731, 779
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
Bsp19I CCATGG 2 cut(s) 633, 1104
BspANI GGCC 2 cut(s) 461, 1081
BspCNI CTCAG 2 cut(s) 307, 346
BspDI ATCGAT 1 cut(s) 1158
BspEI TCCGGA 1 cut(s) 287
BspLI GGNNCC 4 cut(s) 121, 603, 604, 674
BspPI GGATC 4 cut(s) 594, 891, 935, 1061
BspT104I TTCGAA 1 cut(s) 306
BsrI ACTGG 2 cut(s) 456, 462
BssECI CCNNGG 2 cut(s) 633, 1104
BssMI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
BssT1I CCWWGG 2 cut(s) 633, 1104
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 4 cut(s) 709, 879, 1151, 1216
Bst6I CTCTTC 1 cut(s) 324
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 295, 472
BstDEI CTNAG 2 cut(s) 315, 354
BstDSI CCRYGG 2 cut(s) 633, 1104
BstF5I GGATG 2 cut(s) 34, 491
BstKTI GATC 7 cut(s) 19, 589, 655, 772, 886, 930, 1069
BstMAI GTCTC 2 cut(s) 1008, 1122
BstMBI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
BstMWI GCNNNNNNNGC 6 cut(s) 93, 435, 467, 543, 564, 1058
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstSLI GKGCMC 1 cut(s) 731
BstV1I GCAGC 6 cut(s) 222, 266, 749, 752, 855, 1108
BstV2I GAAGAC 2 cut(s) 136, 454
BstX2I RGATCY 2 cut(s) 586, 1066
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 2 cut(s) 586, 1066
Bsu15I ATCGAT 1 cut(s) 1158
BsuRI GGCC 2 cut(s) 461, 1081
BsuTUI ATCGAT 1 cut(s) 1158
BtgI CCRYGG 2 cut(s) 633, 1104
BtsCI GGATG 2 cut(s) 34, 491
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 2 cut(s) 383, 469
Cac8I GCNNGC 2 cut(s) 295, 472
CaiI CAGNNNCTG 1 cut(s) 1121
Cfr13I GGNCC 2 cut(s) 602, 1108
ClaI ATCGAT 1 cut(s) 1158
CseI GACGC 1 cut(s) 990
DdeI CTNAG 2 cut(s) 315, 354
DpnI GATC 7 cut(s) 18, 588, 654, 771, 885, 929, 1068
DpnII GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 3 cut(s) 181, 358, 777
Eco130I CCWWGG 2 cut(s) 633, 1104
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 3 cut(s) 183, 360, 779
Eco47I GGWCC 2 cut(s) 602, 1108
Eco53kI GAGCTC 3 cut(s) 181, 358, 777
EcoICRI GAGCTC 3 cut(s) 181, 358, 777
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 633, 1104
EcoT38I GRGCYC 3 cut(s) 183, 360, 779
ErhI CCWWGG 2 cut(s) 633, 1104
Esp3I CGTCTC 1 cut(s) 1008
FalI AAGNNNNNCTT 4 cut(s) 375, 407, 439, 471
FaqI GGGAC 1 cut(s) 588
FauNDI CATATG 1 cut(s) 1135
FbaI TGATCA 2 cut(s) 652, 769
Fnu4HI GCNGC 6 cut(s) 211, 255, 738, 741, 844, 1122
FokI GGATG 2 cut(s) 41, 478
FriOI GRGCYC 3 cut(s) 183, 360, 779
Fsp4HI GCNGC 6 cut(s) 211, 255, 738, 741, 844, 1122
FspBI CTAG 4 cut(s) 531, 593, 1094, 1169
GluI GCNGC 6 cut(s) 211, 255, 738, 741, 844, 1122
HaeIII GGCC 2 cut(s) 461, 1081
HapII CCGG 2 cut(s) 288, 395
HgaI GACGC 1 cut(s) 990
HinfI GANTC 5 cut(s) 44, 713, 805, 1029, 1130
HpaII CCGG 2 cut(s) 288, 395
HphI GGTGA 3 cut(s) 50, 67, 637
Hpy166II GTNNAC 3 cut(s) 58, 729, 875
Hpy188I TCNGA 4 cut(s) 355, 718, 774, 822
Hpy188III TCNNGA 5 cut(s) 20, 288, 361, 767, 1169
Hpy8I GTNNAC 3 cut(s) 58, 729, 875
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 4 cut(s) 237, 492, 924, 1092
HpyCH4III ACNGT 4 cut(s) 709, 879, 1151, 1216
HpyCH4V TGCA 5 cut(s) 254, 438, 729, 737, 789
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 435, 467, 543, 564, 1058
HpyF3I CTNAG 2 cut(s) 315, 354
KflI GGGWCCC 1 cut(s) 602
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 652, 769
Kzo9I GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
LmnI GCTCC 2 cut(s) 290, 563
Lsp1109I GCAGC 6 cut(s) 222, 266, 749, 752, 855, 1108
LweI GCATC 3 cut(s) 709, 899, 1108
MaeI CTAG 4 cut(s) 531, 593, 1094, 1169
MaeIII GTNAC 3 cut(s) 99, 524, 637
MalI GATC 7 cut(s) 18, 588, 654, 771, 885, 929, 1068
MboI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
MboII GAAGA 6 cut(s) 136, 341, 459, 581, 800, 1190
MflI RGATCY 2 cut(s) 586, 1066
MhlI GDGCHC 5 cut(s) 183, 295, 360, 731, 779
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 3 cut(s) 298, 403, 607
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 3 cut(s) 53, 1038, 1124
MmeI TCCRAC 3 cut(s) 837, 861, 1006
Mox20I TGGCCA 1 cut(s) 461
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 461
MseI TTAA 4 cut(s) 33, 135, 1076, 1264
MslI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 2 cut(s) 288, 395
MspR9I CCNGG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 6 cut(s) 93, 435, 467, 543, 564, 1058
NcoI CCATGG 2 cut(s) 633, 1104
NdeI CATATG 1 cut(s) 1135
NdeII GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
NlaIV GGNNCC 4 cut(s) 121, 603, 604, 674
NmuCI GTSAC 1 cut(s) 524
NspV TTCGAA 1 cut(s) 306
PceI AGGCCT 1 cut(s) 1081
PctI GAATGC 1 cut(s) 510
PfeI GAWTC 2 cut(s) 713, 805
PflFI GACNNNGTC 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 6 cut(s) 212, 256, 739, 742, 845, 1123
PleI GAGTC 3 cut(s) 52, 1037, 1124
PpsI GAGTC 3 cut(s) 52, 1037, 1124
PpuMI RGGWCCY 1 cut(s) 602
Psp124BI GAGCTC 3 cut(s) 183, 360, 779
Psp5II RGGWCCY 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 4 cut(s) 121, 603, 604, 674
PspPI GGNCC 2 cut(s) 602, 1108
PspPPI RGGWCCY 1 cut(s) 602
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 2 cut(s) 586, 1066
PsyI GACNNNGTC 1 cut(s) 349
RseI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
SacI GAGCTC 3 cut(s) 183, 360, 779
SaqAI TTAA 4 cut(s) 33, 135, 1076, 1264
SatI GCNGC 6 cut(s) 211, 255, 738, 741, 844, 1122
Sau3AI GATC 7 cut(s) 16, 586, 652, 769, 883, 927, 1066
Sau96I GGNCC 2 cut(s) 602, 1108
SchI GAGTC 3 cut(s) 53, 1038, 1124
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 5 cut(s) 183, 295, 360, 731, 779
SfaNI GCATC 3 cut(s) 709, 899, 1108
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 2 cut(s) 602, 1108
SmiMI CAYNNNNRTG 5 cut(s) 263, 269, 752, 939, 1160
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 3 cut(s) 298, 403, 607
SseBI AGGCCT 1 cut(s) 1081
SspI AATATT 1 cut(s) 235
SspMI CTAG 4 cut(s) 531, 593, 1094, 1169
SstI GAGCTC 3 cut(s) 183, 360, 779
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 633, 1104
TaaI ACNGT 4 cut(s) 709, 879, 1151, 1216
TaqI TCGA 5 cut(s) 306, 643, 695, 711, 1158
TasI AATT 3 cut(s) 298, 403, 607
TfiI GAWTC 2 cut(s) 713, 805
Tru1I TTAA 4 cut(s) 33, 135, 1076, 1264
Tru9I TTAA 4 cut(s) 33, 135, 1076, 1264
TscAI CASTG 2 cut(s) 383, 469
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 6 cut(s) 210, 254, 737, 740, 843, 1121
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 7 cut(s) 40, 253, 287, 480, 842, 1190, 1242
TspRI CASTG 2 cut(s) 383, 469
Tth111I GACNNNGTC 1 cut(s) 349
VneI GTGCAC 1 cut(s) 727
VpaK11BI GGWCC 2 cut(s) 602, 1108
XbaI TCTAGA 1 cut(s) 1168
XspI CTAG 4 cut(s) 531, 593, 1094, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.