MD17G1090600.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004787
Physical Location & Seq
Reverse (-)
7536435 .. 7539449
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1090600.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGAAGTTCATTGTGATCTTGAAGTTGATGTCAATGGGGAAGAGTCTTTCATGGTGGACAAGAGAATTCTTGCCTCCTACTCTGGCAAATTGAGCAAATTACTTGGAAAATCAAAAGCCTCCTCAAGAAATCTTAAAGTGGTATTCCATGACTTTCCGGGAGGAGCGGAGAGTTTCAAGCTGATTTCAAGGTTTTGTTACAACAATGGCAGCATTTACATAACTCCTTCCAACATTTCCCTCCTTTACTGCGCTGCGCAGTTCATGGAAATGAACAATTCTGTCGCCAGAAAACATAATTTGTTAGAAAAAGCGGAGAAATCATTTCAAGATATCAGGTATTGGTCATGGTCTGAGCTTTTGACGGCTTTGAAGCAGTGCCAGGATTTGCTTCCGGTTGCGAATTCTTTGAGTCTAGTTGAGAAATGCTTGGATACCCTCATTGCGAGATTGCATTTGACAAGCGAAGCAAGTCCTTGTCCTTCGACTTCATCCCCCGATAGCTCCGGTATTCGGTTTTCATGTGATACTAGAAGCACTGAGAGTTTGAAAACTAGCTTCTCTCGCACACCCTGGTGGTTCGAGGATCTTCTAGTTTTAGGTCCAAATTTGGTTGAAATGCTTGTCAAGGCCATGGTTTCGCGAAAACTCGATCATGTTATCATTAGTAGGTTCCTCTTTTATTACCAGAAATCGAAGTTTTACACGGTCAAATCCGATACCAAGCGCACCATTGCTGAAACGGTCATTGAGATGCTGTATATGCTTGATCAGAGCTGTGTTTCATGCAAGAGCTTATTCGGGATTCTTCGAGTTTCGCTGAATTTTAACTTAAACAGAAGTATCAGGAATAAGTTGGAGAATATGATTGGTTCACAGCTAGATCAAGCAACATTGGACAATTTGCTTGTTCCATCCCCACAAGGAATCAATTACTTGTATGATGTCAATCTTGTTCTAAGGTTTTTGAAATCATTTCTGCGTGATGGAGGAACCTGTCAAGCCTCTCCAATCCGATTAAGGAAAGTTTCTGGCTTGATTGATTTGTATATAGCAGAAGTAGCGCCAGATCCTTGTTTGAAGCCTTCAAAGTTTCTGGCTTTAGCTATAGCCCTGCCAGATTCGGCAAGAGACTCCTATGATGAGCTCTACCATGCCATAGACATGTATCTACAGGTGCACGCAGGATTGTCCGAAGACAAAAAGATGAAAATATGTTGTGTACTGAACTATGAGAAGCTCTCGGCAGAGACTTGCATACACCTTTCAAAGAACACAAAATTTCCATCAAAATCTGCAGTCCAAGCTCTTGTCTCTCAGCAATCCAAGCTCAAAAAGTTACTCCAAACACCCAACAACTTTAATTCCCATGCCACCTCCCCTTATAGCGCCACTACTGAGGCAAAAAATTGGGGATTAAGGAAAGATGATGCCAACGAACAACATGTGCTTTATGCCGGAAAACTCGGTCTCTCAACTGACAACGAGAAGCTCAGAGCACATGTGCAAGGCATGCAATGCAGGGTCATGGAATTGGAGAAAGTTTGCAAGAAAATGCAATCTCAAATGGCAAAGTTTACAAAATCGAAAGCATCAAGCCACAGCCATACAAGATCTTTGCCCAAACTTTGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

546

Amino Acids

61.39

Weight (kDa)

9.03

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 192 - 419 2.6e-63 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 643
AciI CCGC 2 cut(s) 167, 314
AclWI GGATC 2 cut(s) 594, 1064
AcsI RAATTY 5 cut(s) 66, 403, 607, 823, 1280
AfaI GTAC 1 cut(s) 1224
AfiI CCNNNNNNNGG 1 cut(s) 513
AflIII ACRYGT 3 cut(s) 1164, 1444, 1501
AjnI CCWGG 2 cut(s) 381, 572
AleI CACNNNNGTG 1 cut(s) 574
Alw21I GWGCWC 3 cut(s) 1149, 1182, 1501
Alw26I GTCTC 4 cut(s) 1125, 1244, 1318, 1475
Alw44I GTGCAC 1 cut(s) 1178
AlwI GGATC 2 cut(s) 594, 1064
AoxI GGCC 1 cut(s) 630
ApaLI GTGCAC 1 cut(s) 1178
ApeKI GCWGC 2 cut(s) 210, 254
ApoI RAATTY 5 cut(s) 66, 403, 607, 823, 1280
AspLEI GCGC 5 cut(s) 254, 259, 729, 1066, 1391
AspS9I GGNCC 1 cut(s) 602
AsuC2I CCSGG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 1182
BaeI ACNNNNGTAYC 2 cut(s) 826, 859
BanII GRGCYC 1 cut(s) 1149
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 3 cut(s) 1149, 1182, 1501
BbvI GCAGC 2 cut(s) 222, 241
BccI CCATC 3 cut(s) 922, 980, 1294
BceAI ACGGC 1 cut(s) 381
BciT130I CCWGG 2 cut(s) 383, 574
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 4 cut(s) 1125, 1244, 1318, 1475
BfaI CTAG 5 cut(s) 416, 531, 555, 593, 881
BfmI CTRYAG 3 cut(s) 1107, 1172, 1296
BfoI RGCGCY 2 cut(s) 1067, 1392
BfuI GTATCC 1 cut(s) 427
BglII AGATCT 1 cut(s) 1613
BisI GCNGC 2 cut(s) 211, 255
BlsI GCNGC 2 cut(s) 212, 256
Bme1390I CCNGG 3 cut(s) 159, 383, 574
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 2 cut(s) 674, 994
BmrFI CCNGG 3 cut(s) 159, 383, 574
BmsI GCATC 3 cut(s) 744, 1420, 1601
BpiI GAAGAC 1 cut(s) 1203
BplI GAGNNNNNCTC 2 cut(s) 1226, 1258
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 1475
BsaJI CCNNGG 2 cut(s) 572, 633
BsaWI WCCGGW 2 cut(s) 394, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse3DI GCAATG 3 cut(s) 441, 732, 1523
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 2 cut(s) 383, 574
BseDI CCNNGG 2 cut(s) 572, 633
BseGI GGATG 2 cut(s) 491, 914
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 3 cut(s) 441, 732, 1523
BseMII CTCAG 5 cut(s) 345, 531, 1331, 1389, 1507
BseRI GAGGAG 2 cut(s) 112, 177
BseSI GKGCMC 1 cut(s) 1182
BseXI GCAGC 2 cut(s) 222, 241
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 1 cut(s) 632
BsiHKAI GWGCWC 3 cut(s) 1149, 1182, 1501
BsiSI CCGG 4 cut(s) 158, 395, 507, 1458
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 4 cut(s) 1125, 1244, 1318, 1475
BsnI GGCC 1 cut(s) 632
Bso31I GGTCTC 1 cut(s) 1475
Bsp1286I GDGCHC 3 cut(s) 1149, 1182, 1501
Bsp143I GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
Bsp19I CCATGG 1 cut(s) 633
Bsp68I TCGCGA 1 cut(s) 643
BspACI CCGC 2 cut(s) 167, 314
BspANI GGCC 1 cut(s) 632
BspCNI CTCAG 5 cut(s) 346, 532, 1330, 1390, 1506
BspFNI CGCG 1 cut(s) 643
BspHI TCATGA 1 cut(s) 1634
BspLI GGNNCC 2 cut(s) 674, 994
BspMAI CTGCAG 1 cut(s) 1300
BspPI GGATC 2 cut(s) 594, 1064
BspTNI GGTCTC 1 cut(s) 1475
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 3 cut(s) 441, 732, 1523
BssECI CCNNGG 2 cut(s) 572, 633
BssMI GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 2 cut(s) 383, 574
Bst4CI ACNGT 2 cut(s) 709, 745
Bst6I CTCTTC 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 1513, 1518
BstC8I GCNNGC 2 cut(s) 1182, 1514
BstDEI CTNAG 6 cut(s) 354, 540, 959, 1317, 1398, 1493
BstDSI CCRYGG 1 cut(s) 633
BstF5I GGATG 2 cut(s) 491, 914
BstFNI CGCG 1 cut(s) 643
BstH2I RGCGCY 2 cut(s) 1067, 1392
BstHHI GCGC 5 cut(s) 254, 259, 729, 1066, 1391
BstKTI GATC 7 cut(s) 19, 589, 655, 772, 886, 1072, 1616
BstMAI GTCTC 4 cut(s) 1125, 1244, 1318, 1475
BstMBI GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
BstMWI GCNNNNNNNGC 8 cut(s) 93, 564, 763, 1061, 1304, 1327, 1513, 1518
BstNI CCWGG 2 cut(s) 383, 574
BstNSI RCATGY 4 cut(s) 1168, 1448, 1505, 1516
BstSCI CCNGG 3 cut(s) 157, 381, 572
BstSFI CTRYAG 3 cut(s) 1107, 1172, 1296
BstSLI GKGCMC 1 cut(s) 1182
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 2 cut(s) 222, 241
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 3 cut(s) 586, 1069, 1613
BstYI RGATCY 3 cut(s) 586, 1069, 1613
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 632
BtgI CCRYGG 1 cut(s) 633
BtsCI GGATG 2 cut(s) 491, 914
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 2 cut(s) 383, 537
BtuMI TCGCGA 1 cut(s) 643
Cac8I GCNNGC 2 cut(s) 1182, 1514
CciI TCATGA 1 cut(s) 1634
CfoI GCGC 5 cut(s) 254, 259, 729, 1066, 1391
Cfr13I GGNCC 1 cut(s) 602
Csp6I GTAC 1 cut(s) 1223
CviQI GTAC 1 cut(s) 1223
DdeI CTNAG 6 cut(s) 354, 540, 959, 1317, 1398, 1493
DpnI GATC 7 cut(s) 18, 588, 654, 771, 885, 1071, 1615
DpnII GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 1147
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 1 cut(s) 1149
Eco31I GGTCTC 1 cut(s) 1475
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 1147
EcoICRI GAGCTC 1 cut(s) 1147
EcoRI GAATTC 2 cut(s) 66, 403
EcoRII CCWGG 2 cut(s) 381, 572
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 1149
ErhI CCWWGG 1 cut(s) 633
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 2 cut(s) 211, 255
FokI GGATG 2 cut(s) 478, 901
FriOI GRGCYC 1 cut(s) 1149
Fsp4HI GCNGC 2 cut(s) 211, 255
FspBI CTAG 5 cut(s) 416, 531, 555, 593, 881
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 5 cut(s) 253, 258, 728, 1065, 1390
GluI GCNGC 2 cut(s) 211, 255
HaeII RGCGCY 2 cut(s) 1067, 1392
HaeIII GGCC 1 cut(s) 632
HapII CCGG 4 cut(s) 158, 395, 507, 1458
HhaI GCGC 5 cut(s) 254, 259, 729, 1066, 1391
Hin6I GCGC 5 cut(s) 252, 257, 727, 1064, 1389
HinP1I GCGC 5 cut(s) 252, 257, 727, 1064, 1389
HinfI GANTC 6 cut(s) 44, 412, 805, 927, 1121, 1133
HpaII CCGG 4 cut(s) 158, 395, 507, 1458
Hpy166II GTNNAC 5 cut(s) 58, 875, 1180, 1223, 1578
Hpy188I TCNGA 6 cut(s) 355, 718, 774, 1016, 1195, 1496
Hpy188III TCNNGA 7 cut(s) 20, 126, 329, 642, 802, 847, 1635
Hpy8I GTNNAC 5 cut(s) 58, 875, 1180, 1223, 1578
HpyAV CCTTC 3 cut(s) 237, 492, 1095
HpyCH4III ACNGT 2 cut(s) 709, 745
HpyF10VI GCNNNNNNNGC 8 cut(s) 93, 564, 763, 1061, 1304, 1327, 1513, 1518
HpyF3I CTNAG 6 cut(s) 354, 540, 959, 1317, 1398, 1493
HspAI GCGC 5 cut(s) 252, 257, 727, 1064, 1389
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
LmnI GCTCC 2 cut(s) 164, 509
Lsp1109I GCAGC 2 cut(s) 222, 241
LweI GCATC 3 cut(s) 744, 1420, 1601
MaeI CTAG 5 cut(s) 416, 531, 555, 593, 881
MaeIII GTNAC 2 cut(s) 197, 1338
MalI GATC 7 cut(s) 18, 588, 654, 771, 885, 1071, 1615
MbiI CCGCTC 1 cut(s) 167
MboI GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
MboII GAAGA 4 cut(s) 53, 581, 800, 1208
MflI RGATCY 3 cut(s) 586, 1069, 1613
MhlI GDGCHC 3 cut(s) 1149, 1182, 1501
MlyI GAGTC 3 cut(s) 53, 421, 1127
MmeI TCCRAC 2 cut(s) 255, 837
MseI TTAA 6 cut(s) 135, 828, 833, 1019, 1362, 1418
MslI CAYNNNNRTG 4 cut(s) 269, 574, 752, 1163
MspI CCGG 4 cut(s) 158, 395, 507, 1458
MspR9I CCNGG 3 cut(s) 159, 383, 574
MteI GCGCNGCGC 1 cut(s) 255
MvaI CCWGG 2 cut(s) 383, 574
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 8 cut(s) 93, 564, 763, 1061, 1304, 1327, 1513, 1518
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 633
NdeII GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
NlaIV GGNNCC 2 cut(s) 674, 994
NmeAIII GCCGAG 1 cut(s) 1223
NruI TCGCGA 1 cut(s) 643
NsbI TGCGCA 1 cut(s) 258
NspI RCATGY 4 cut(s) 1168, 1448, 1505, 1516
OliI CACNNNNGTG 1 cut(s) 574
PaeI GCATGC 1 cut(s) 1516
PagI TCATGA 1 cut(s) 1634
PciI ACATGT 3 cut(s) 1164, 1444, 1501
PfeI GAWTC 3 cut(s) 805, 927, 1121
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 2 cut(s) 212, 256
PleI GAGTC 3 cut(s) 52, 420, 1127
PpsI GAGTC 3 cut(s) 52, 420, 1127
PscI ACATGT 3 cut(s) 1164, 1444, 1501
Psp124BI GAGCTC 1 cut(s) 1149
Psp6I CCWGG 2 cut(s) 381, 572
PspGI CCWGG 2 cut(s) 381, 572
PspN4I GGNNCC 2 cut(s) 674, 994
PspPI GGNCC 1 cut(s) 602
PstI CTGCAG 1 cut(s) 1300
PsuI RGATCY 3 cut(s) 586, 1069, 1613
RruI TCGCGA 1 cut(s) 643
RsaI GTAC 1 cut(s) 1224
RsaNI GTAC 1 cut(s) 1223
RseI CAYNNNNRTG 4 cut(s) 269, 574, 752, 1163
SacI GAGCTC 1 cut(s) 1149
SaqAI TTAA 6 cut(s) 135, 828, 833, 1019, 1362, 1418
SatI GCNGC 2 cut(s) 211, 255
Sau3AI GATC 7 cut(s) 16, 586, 652, 769, 883, 1069, 1613
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 3 cut(s) 53, 421, 1127
ScrFI CCNGG 3 cut(s) 159, 383, 574
SduI GDGCHC 3 cut(s) 1149, 1182, 1501
SfaNI GCATC 3 cut(s) 744, 1420, 1601
SfcI CTRYAG 3 cut(s) 1107, 1172, 1296
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 4 cut(s) 269, 574, 752, 1163
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
SphI GCATGC 1 cut(s) 1516
SsiI CCGC 2 cut(s) 167, 314
SspMI CTAG 5 cut(s) 416, 531, 555, 593, 881
SstI GAGCTC 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 157, 381, 572
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 2 cut(s) 709, 745
TaqI TCGA 6 cut(s) 485, 582, 651, 695, 811, 1586
TaqII GACCGA 1 cut(s) 1457
TatI WGTACW 1 cut(s) 1222
TfiI GAWTC 3 cut(s) 805, 927, 1121
Tru1I TTAA 6 cut(s) 135, 828, 833, 1019, 1362, 1418
Tru9I TTAA 6 cut(s) 135, 828, 833, 1019, 1362, 1418
TscAI CASTG 2 cut(s) 383, 544
TseI GCWGC 2 cut(s) 210, 254
TspDTI ATGAA 8 cut(s) 40, 253, 287, 480, 510, 774, 1223, 1623
TspRI CASTG 2 cut(s) 383, 544
VneI GTGCAC 1 cut(s) 1178
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 5 cut(s) 66, 403, 607, 823, 1280
XceI RCATGY 4 cut(s) 1168, 1448, 1505, 1516
XspI CTAG 5 cut(s) 416, 531, 555, 593, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.