AT3G22290

Endoplasmic reticulum-Golgi intermediate compartment protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012526
Physical Location & Seq
Forward (+)
7881547 .. 7885592
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22290.2

Sequence Viewer

Length: 1065 bp
ATGGGTGTGAAGCAGGCTCTAAGGAGCATTGATGCGTTTCCAAGAGCAGAGGATCATTTGCTTCAGAAGACACAATCTGGCGCTGTTGTATCTATTGTTGGACTTCTTATAATGGCTACTTTGTTCTTACACGAGCTGAGTTATTATCTTAACACACTTACAGTGCACCAGATGTCAGTGGACTTAAAGCGTGGAGAAACTCTTCCAATTCATGTAAATATGACGTTTCCATCTTTACCTTGTGATGTACTGAGCGTAGATGCTATCGACATGTCAGGGAAGCATGAAGTGGATCTTGATACAAACATTTGGAAGCTCCGTCTCAACAGTCATGGTCATATCATAGGCACAGAATATATATCTGATCTTGTTGAAAAGGGGCATGAGCATGGCCACTCACCTCACAAGCATGATGGCAAAGAAGAACACAAGAATGAGACTGAAACCGAAGCATTGAATATTCTTGGCTTTGACCAAGCTGCTGAGACTATGATTAAAAAAGTGAAGCAAGCATTGGCAGATGGAGAAGGATGTCGGGTTTATGGTGTCCTAGATGTACAACGGGTTGCCGGAAACTTCCATATTTCAGTTCATGGGCTGAACATCTATGTTGCTCAGATGATATTTGGCGGGTCCAAAAACGTAAACGTGAGCCATATGATTCACGATCTATCATTTGGGCCGAAATACCCCGGAATTCACAACCCACTTGATGACACCAACCGAATACTCCATGACACCAGTGGAACATTCAAATACTATATTAAGATTGTTCCCACAGAATATAGATATCTTTCAAAAGACGTACTGTCAACAAATCAGTACTCTGTAACTGAATATTTCACGCCAATGACTGAATTCGACCGTACATGGCCAGCTGTTTACTTCTTATATGATCTTTCACCTATCACTGTGACCATCAAGGAAGAACGCCGTAGTTTTCTCCACTTAATCACTCGTCTTTGCGCTGTATTAGGCGGTACATTTGCTTTGACAGGAATGTTAGATCGTTGGATGTTCCGCTTTATTGAATCTTTCAACAAGAAGCCGAGTACTAGAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

354

Amino Acids

40.09

Weight (kDa)

6.36

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 96 1.8e-27 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 156 - 336 5e-46 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22290 AT3G22290
fragaria_vesca FvH4_6g40110 FvH4_6g40110 FvH4_6g40110 FvH4_6g40110
malus_domestica MD09G1135200.v1.1 MD17G1124200.v1.1
prunus_persica Prupe.3G191500_v2.0.a1
pyrus_communis pycom09g05630 pycom17g11560
rosa_chinensis RchiOBHm_Chr2g0154741
rosa_laevigata RLG00000020799
rosa_multiflora Rmu_sc0001027.1_g000004
rosa_roxburghii Rroxscaffold_2G00094140
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2BG521200 Rh2CG495500
rosa_wichuraiana Rw2G041970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
AciI CCGC 3 cut(s) 630, 978, 1021
AclWI GGATC 2 cut(s) 60, 300
AcoI YGGCCR 2 cut(s) 391, 872
AcsI RAATTY 2 cut(s) 696, 857
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 7 cut(s) 249, 558, 807, 824, 868, 982, 1054
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 6 cut(s) 374, 457, 754, 798, 1031, 1039
AhdI GACNNNNNGTC 1 cut(s) 808
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 4 cut(s) 136, 316, 479, 878
AluI AGCT 4 cut(s) 136, 316, 479, 878
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 326, 431, 479
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 2 cut(s) 60, 300
AoxI GGCC 3 cut(s) 391, 680, 872
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 696, 857
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 83, 968
AspS9I GGNCC 2 cut(s) 633, 680
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 390, 894
AvaII GGWCC 1 cut(s) 633
BaeGI GKGCMC 1 cut(s) 168
BalI TGGCCA 2 cut(s) 393, 874
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 466
BccI CCATC 4 cut(s) 238, 407, 515, 926
BceAI ACGGC 1 cut(s) 918
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 326, 431, 479
BfaI CTAG 2 cut(s) 551, 1056
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 1 cut(s) 480
BlsI GCNGC 1 cut(s) 481
BmcAI AGTACT 2 cut(s) 824, 1054
Bme1390I CCNGG 1 cut(s) 693
Bme18I GGWCC 1 cut(s) 633
BmeRI GACNNNNNGTC 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 633, 680
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 1 cut(s) 693
BmsI GCATC 2 cut(s) 22, 250
BpiI GAAGAC 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 693
BsaJI CCNNGG 1 cut(s) 691
Bse1I ACTGG 1 cut(s) 741
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 2 cut(s) 536, 1020
BseMII CTCAG 4 cut(s) 128, 242, 474, 629
BseNI ACTGG 1 cut(s) 741
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 865
BshFI GGCC 3 cut(s) 393, 682, 874
BsiEI CGRYCG 1 cut(s) 865
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 2 cut(s) 570, 693
BsmAI GTCTC 3 cut(s) 326, 431, 479
BsmBI CGTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 393, 682, 874
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
BspACI CCGC 3 cut(s) 630, 978, 1021
BspANI GGCC 3 cut(s) 393, 682, 874
BspCNI CTCAG 4 cut(s) 129, 243, 475, 628
BspLI GGNNCC 1 cut(s) 634
BspPI GGATC 2 cut(s) 60, 300
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
BssSI CACGAG 1 cut(s) 131
Bst2BI CACGAG 1 cut(s) 131
Bst4CI ACNGT 5 cut(s) 163, 329, 810, 866, 913
Bst6I CTCTTC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 15, 510, 876
BstDEI CTNAG 5 cut(s) 20, 137, 251, 483, 615
BstF5I GGATG 2 cut(s) 536, 1020
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 968
BstKTI GATC 6 cut(s) 55, 295, 367, 670, 898, 1009
BstMAI GTCTC 3 cut(s) 326, 431, 479
BstMBI GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
BstMCI CGRYCG 1 cut(s) 865
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 1 cut(s) 691
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 292
BstYI RGATCY 1 cut(s) 292
BsuRI GGCC 3 cut(s) 393, 682, 874
BtsCI GGATG 2 cut(s) 536, 1020
BtsIMutI CAGTG 4 cut(s) 168, 183, 748, 909
Cac8I GCNNGC 3 cut(s) 15, 510, 876
CfoI GCGC 2 cut(s) 83, 968
Cfr13I GGNCC 2 cut(s) 633, 680
Csp6I GTAC 7 cut(s) 248, 557, 806, 823, 867, 981, 1053
CviQI GTAC 7 cut(s) 248, 557, 806, 823, 867, 981, 1053
DdeI CTNAG 5 cut(s) 20, 137, 251, 483, 615
DpnI GATC 6 cut(s) 54, 294, 366, 669, 897, 1008
DpnII GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
DriI GACNNNNNGTC 1 cut(s) 808
EaeI YGGCCR 2 cut(s) 391, 872
Eam1104I CTCTTC 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 808
EarI CTCTTC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 791
Eco47I GGWCC 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 47
EcoRI GAATTC 2 cut(s) 696, 857
EcoRV GATATC 1 cut(s) 791
Esp3I CGTCTC 1 cut(s) 326
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FauI CCCGC 1 cut(s) 623
FauNDI CATATG 1 cut(s) 657
Fnu4HI GCNGC 1 cut(s) 480
FokI GGATG 2 cut(s) 543, 1027
Fsp4HI GCNGC 1 cut(s) 480
FspBI CTAG 2 cut(s) 551, 1056
GlaI GCGC 2 cut(s) 82, 967
GluI GCNGC 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 3 cut(s) 393, 682, 874
HapII CCGG 2 cut(s) 570, 693
HhaI GCGC 2 cut(s) 83, 968
Hin6I GCGC 2 cut(s) 81, 966
HinP1I GCGC 2 cut(s) 81, 966
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HinfI GANTC 2 cut(s) 661, 1031
HpaII CCGG 2 cut(s) 570, 693
HphI GGTGA 2 cut(s) 390, 894
Hpy166II GTNNAC 5 cut(s) 166, 181, 646, 813, 883
Hpy188I TCNGA 3 cut(s) 66, 364, 618
Hpy188III TCNNGA 2 cut(s) 296, 665
Hpy8I GTNNAC 5 cut(s) 166, 181, 646, 813, 883
HpyAV CCTTC 1 cut(s) 521
HpyCH4III ACNGT 5 cut(s) 163, 329, 810, 866, 913
HpyCH4IV ACGT 4 cut(s) 224, 642, 648, 804
HpyCH4V TGCA 1 cut(s) 166
HpyF3I CTNAG 5 cut(s) 20, 137, 251, 483, 615
HpySE526I ACGT 4 cut(s) 224, 642, 648, 804
HspAI GCGC 2 cut(s) 81, 966
Kzo9I GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
LmnI GCTCC 2 cut(s) 24, 321
LpnPI CCDG 8 cut(s) 63, 182, 261, 583, 706, 754, 888, 981
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 2 cut(s) 22, 250
MaeI CTAG 2 cut(s) 551, 1056
MaeII ACGT 4 cut(s) 224, 642, 648, 804
MaeIII GTNAC 2 cut(s) 829, 913
MalI GATC 6 cut(s) 54, 294, 366, 669, 897, 1008
MboI GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
MboII GAAGA 4 cut(s) 79, 194, 434, 938
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 2 cut(s) 393, 874
MluCI AATT 3 cut(s) 207, 696, 857
MluNI TGGCCA 2 cut(s) 393, 874
MmeI TCCRAC 2 cut(s) 79, 992
MnlI CCTC 2 cut(s) 43, 411
Mox20I TGGCCA 2 cut(s) 393, 874
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 2 cut(s) 393, 874
MseI TTAA 5 cut(s) 150, 185, 495, 765, 950
MslI CAYNNNNRTG 4 cut(s) 387, 408, 432, 848
Msp20I TGGCCA 2 cut(s) 393, 874
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 2 cut(s) 570, 693
MspR9I CCNGG 1 cut(s) 693
NciI CCSGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 657
NdeII GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
NlaIV GGNNCC 1 cut(s) 634
NmuCI GTSAC 1 cut(s) 913
NspI RCATGY 1 cut(s) 274
PciI ACATGT 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 2 cut(s) 661, 1031
PkrI GCNGC 1 cut(s) 481
PscI ACATGT 1 cut(s) 270
PsiI TTATAA 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 2 cut(s) 633, 680
PsuI RGATCY 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 7 cut(s) 249, 558, 807, 824, 868, 982, 1054
RsaNI GTAC 7 cut(s) 248, 557, 806, 823, 867, 981, 1053
RseI CAYNNNNRTG 4 cut(s) 387, 408, 432, 848
SaqAI TTAA 5 cut(s) 150, 185, 495, 765, 950
SatI GCNGC 1 cut(s) 480
Sau3AI GATC 6 cut(s) 52, 292, 364, 667, 895, 1006
Sau96I GGNCC 2 cut(s) 633, 680
ScaI AGTACT 2 cut(s) 824, 1054
ScrFI CCNGG 1 cut(s) 693
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 2 cut(s) 22, 250
SinI GGWCC 1 cut(s) 633
SmiMI CAYNNNNRTG 4 cut(s) 387, 408, 432, 848
Sse9I AATT 3 cut(s) 207, 696, 857
SsiI CCGC 3 cut(s) 630, 978, 1021
SspI AATATT 2 cut(s) 460, 839
SspMI CTAG 2 cut(s) 551, 1056
StyD4I CCNGG 1 cut(s) 691
TaaI ACNGT 5 cut(s) 163, 329, 810, 866, 913
TaiI ACGT 4 cut(s) 227, 645, 651, 807
TaqI TCGA 2 cut(s) 267, 861
TasI AATT 3 cut(s) 207, 696, 857
TatI WGTACW 4 cut(s) 247, 556, 822, 1052
TfiI GAWTC 2 cut(s) 661, 1031
Tru1I TTAA 5 cut(s) 150, 185, 495, 765, 950
Tru9I TTAA 5 cut(s) 150, 185, 495, 765, 950
TscAI CASTG 4 cut(s) 168, 183, 748, 916
TseFI GTSAC 1 cut(s) 913
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 1 cut(s) 913
TspDTI ATGAA 3 cut(s) 200, 300, 581
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 4 cut(s) 168, 183, 748, 916
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 633
XapI RAATTY 2 cut(s) 696, 857
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 551, 1056
ZrmI AGTACT 2 cut(s) 824, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.