Rh2BG521200

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012526
Physical Location & Seq
Forward (+)
73184588 .. 73187973
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG521200.1

Sequence Viewer

Length: 1044 bp
ATGGGGATGAAACAAGCCTTAAAGAGCTTGGATGCGTTTCCTCGAGCCGAGGAGCATTTGTTGCAGAAGACTCAATCTGGGGCTTTTGTTTCTGTTGTTGGTCTGCTCATAATGGCGACGTTGTTTTTGCACGAGCTGAGATATTATCTTTCATCGTATACTGTTCATCAGATGTCTGTGGACTTGAAGCGCGGCGAGACTCTTCCAATTCACATAAACATGACATTTCCGGCTCTACCTTGTGAAGTCCTGAGTGTGGATGCAATTGATATGTCGGGCAAGCATGAAGTGGATCTTGACACAAATATATGGAAACTTCGCCTTAACAGTCATGGACACATCATCGGCACTGAATATTTGTCTGACCTTGTGGAGAAGGAACATTCTAACAACCATGATGATGGTAAAGAGCATCACCAGGAAAACCATTTACATGGAGCTTTTGATCTAGCTGCAGAAGAACTGATCAAGAAAGTAAAGAAAGCATTAGATGATGGAGAAGGATGCAGGGTTTATGGTGTTTTAGATGTCCAAAGGGTTGCTGGAAACTTTCATATATCAGTTCATGGGTTAAACATTTTCGTTGCACAAATGATTTTTGAAGGATCTAAAAATGTAAATGTAAGTCACATTATTCATGACTTGTCATTTGGCCCAAAATATCCAGGGATTCACAACCCGCTTGACGGAACTGAGCGCATTTTGCATGGCACCAGTGGCACATTCAAATATTATATAAAGATTGTTCCAACTGAGTATAGGTATCTCTCAAAGGAAGTTTTGCCAACTAATCAATTCTCTGTGACAGAATATTATTCCCCAATGAAAGAGTATGATAGGTCATGGCCAGCGGTCTACTTCTTGTATGATCTTTCGCCAATTACTGTAACTATCAAAGAAGAACGACGCAAATTTCTTCATTTCATCACTCGGCTTTGTGCAGTATTGGGTGGTACCTTCGCTTTGACAGGAATGCTAGACCGATGGATGTTCAGGTTTGTTGAAGCATTGACTAAACCAAATGCTAGAATTGTACTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

347

Amino Acids

39.6

Weight (kDa)

6.57

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 96 1.3e-28 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 153 - 327 6.5e-46 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22290 AT3G22290
fragaria_vesca FvH4_6g40110 FvH4_6g40110 FvH4_6g40110 FvH4_6g40110
malus_domestica MD09G1135200.v1.1 MD17G1124200.v1.1
prunus_persica Prupe.3G191500_v2.0.a1
pyrus_communis pycom09g05630 pycom17g11560
rosa_chinensis RchiOBHm_Chr2g0154741
rosa_laevigata RLG00000020799
rosa_multiflora Rmu_sc0001027.1_g000004
rosa_roxburghii Rroxscaffold_2G00094140
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2BG521200 Rh2CG495500
rosa_wichuraiana Rw2G041970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 953
AccB1I GGYRCC 2 cut(s) 710, 953
AccI GTMKAC 2 cut(s) 158, 855
AccII CGCG 1 cut(s) 192
AciI CCGC 3 cut(s) 192, 680, 851
AclWI GGATC 2 cut(s) 300, 613
AcoI YGGCCR 1 cut(s) 845
AcsI RAATTY 1 cut(s) 911
AfaI GTAC 2 cut(s) 955, 1035
AfiI CCNNNNNNNGG 2 cut(s) 256, 686
AgsI TTSAA 4 cut(s) 187, 602, 727, 1004
AjnI CCWGG 2 cut(s) 417, 664
AluBI AGCT 4 cut(s) 27, 136, 440, 452
AluI AGCT 4 cut(s) 27, 136, 440, 452
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 2 cut(s) 300, 613
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 2 cut(s) 652, 845
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 911
ArsI GACNNNNNNTTYG 4 cut(s) 109, 141, 632, 664
Asp718I GGTACC 1 cut(s) 953
AspLEI GCGC 2 cut(s) 192, 699
AspS9I GGNCC 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 407
AvaI CYCGRG 1 cut(s) 42
BalI TGGCCA 1 cut(s) 847
BanI GGYRCC 2 cut(s) 710, 953
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 439
BccI CCATC 3 cut(s) 395, 488, 978
BciT130I CCWGG 2 cut(s) 419, 666
BclI TGATCA 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 3 cut(s) 449, 977, 1026
BfmI CTRYAG 1 cut(s) 453
BisI GCNGC 2 cut(s) 193, 453
BlsI GCNGC 2 cut(s) 194, 454
Bme1390I CCNGG 2 cut(s) 419, 666
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 2 cut(s) 712, 955
BmrFI CCNGG 2 cut(s) 419, 666
BmsI GCATC 4 cut(s) 22, 250, 421, 494
BpiI GAAGAC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 48, 665
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 686
Bse1I ACTGG 1 cut(s) 714
BseBI CCWGG 2 cut(s) 419, 666
BseDI CCNNGG 2 cut(s) 48, 665
BseGI GGATG 5 cut(s) 12, 37, 265, 509, 993
BseLI CCNNNNNNNGG 2 cut(s) 256, 686
BseMII CTCAG 4 cut(s) 128, 242, 684, 744
BseNI ACTGG 1 cut(s) 714
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 439
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 2 cut(s) 654, 847
BshNI GGYRCC 2 cut(s) 710, 953
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 256, 686
BsmAI GTCTC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 978
BsnI GGCC 2 cut(s) 654, 847
BsoBI CYCGRG 1 cut(s) 42
Bsp143I GATC 5 cut(s) 292, 445, 465, 605, 868
BspACI CCGC 3 cut(s) 192, 680, 851
BspANI GGCC 2 cut(s) 654, 847
BspCNI CTCAG 4 cut(s) 129, 243, 685, 745
BspFNI CGCG 1 cut(s) 192
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 2 cut(s) 712, 955
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 2 cut(s) 300, 613
BspT107I GGYRCC 2 cut(s) 710, 953
BsrI ACTGG 1 cut(s) 714
BssECI CCNNGG 2 cut(s) 48, 665
BssMI GATC 5 cut(s) 292, 445, 465, 605, 868
BssNAI GTATAC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 131
Bst1107I GTATAC 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 419, 666
Bst4CI ACNGT 3 cut(s) 163, 329, 886
Bst6I CTCTTC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 281, 849
BstDEI CTNAG 4 cut(s) 137, 251, 693, 753
BstF5I GGATG 5 cut(s) 12, 37, 265, 509, 993
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 2 cut(s) 192, 699
BstKTI GATC 5 cut(s) 295, 448, 468, 608, 871
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 5 cut(s) 292, 445, 465, 605, 868
BstMWI GCNNNNNNNGC 3 cut(s) 61, 703, 717
BstNI CCWGG 2 cut(s) 419, 666
BstSCI CCNGG 2 cut(s) 417, 664
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 2 cut(s) 292, 605
BstXI CCANNNNNNTGG 2 cut(s) 401, 434
BstYI RGATCY 2 cut(s) 292, 605
BstZ17I GTATAC 1 cut(s) 159
BsuRI GGCC 2 cut(s) 654, 847
BtsCI GGATG 5 cut(s) 12, 37, 265, 509, 993
BtsIMutI CAGTG 2 cut(s) 348, 721
Cac8I GCNNGC 2 cut(s) 281, 849
CciI TCATGA 1 cut(s) 637
CfoI GCGC 2 cut(s) 192, 699
Cfr13I GGNCC 1 cut(s) 653
CseI GACGC 1 cut(s) 915
Csp6I GTAC 2 cut(s) 954, 1034
CviAII CATG 9 cut(s) 220, 284, 332, 395, 434, 566, 638, 707, 843
CviQI GTAC 2 cut(s) 954, 1034
DdeI CTNAG 4 cut(s) 137, 251, 693, 753
DpnI GATC 5 cut(s) 294, 447, 467, 607, 870
DpnII GATC 5 cut(s) 292, 445, 465, 605, 868
EaeI YGGCCR 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco88I CYCGRG 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 417, 664
FaeI CATG 9 cut(s) 223, 287, 335, 398, 437, 569, 641, 710, 846
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 9 cut(s) 219, 283, 331, 394, 433, 565, 637, 706, 842
FauI CCCGC 1 cut(s) 687
FbaI TGATCA 1 cut(s) 465
FblI GTMKAC 2 cut(s) 158, 855
Fnu4HI GCNGC 2 cut(s) 193, 453
FokI GGATG 5 cut(s) 19, 44, 272, 516, 1000
Fsp4HI GCNGC 2 cut(s) 193, 453
FspBI CTAG 3 cut(s) 449, 977, 1026
GlaI GCGC 2 cut(s) 191, 698
GluI GCNGC 2 cut(s) 193, 453
HaeIII GGCC 2 cut(s) 654, 847
HapII CCGG 1 cut(s) 230
HgaI GACGC 1 cut(s) 915
HhaI GCGC 2 cut(s) 192, 699
Hin1II CATG 9 cut(s) 223, 287, 335, 398, 437, 569, 641, 710, 846
Hin6I GCGC 2 cut(s) 190, 697
HinP1I GCGC 2 cut(s) 190, 697
HinfI GANTC 3 cut(s) 70, 199, 670
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 159, 181, 856
Hpy188I TCNGA 2 cut(s) 171, 364
Hpy188III TCNNGA 4 cut(s) 250, 296, 469, 638
Hpy8I GTNNAC 3 cut(s) 159, 181, 856
Hpy99I CGWCG 2 cut(s) 121, 909
HpyAV CCTTC 4 cut(s) 370, 494, 596, 967
HpyCH4III ACNGT 3 cut(s) 163, 329, 886
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 8 cut(s) 64, 130, 263, 455, 507, 587, 706, 941
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 703, 717
HpyF3I CTNAG 4 cut(s) 137, 251, 693, 753
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 9 cut(s) 223, 287, 335, 398, 437, 569, 641, 710, 846
HspAI GCGC 2 cut(s) 190, 697
KpnI GGTACC 1 cut(s) 957
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 5 cut(s) 292, 445, 465, 605, 868
LmnI GCTCC 2 cut(s) 52, 437
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 4 cut(s) 22, 250, 421, 494
MaeI CTAG 3 cut(s) 449, 977, 1026
MaeII ACGT 1 cut(s) 119
MaeIII GTNAC 3 cut(s) 626, 802, 886
MalI GATC 5 cut(s) 294, 447, 467, 607, 870
MboI GATC 5 cut(s) 292, 445, 465, 605, 868
MboII GAAGA 5 cut(s) 79, 194, 470, 908, 911
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 292, 605
MlsI TGGCCA 1 cut(s) 847
MluCI AATT 6 cut(s) 207, 264, 794, 879, 911, 1029
MluNI TGGCCA 1 cut(s) 847
MlyI GAGTC 2 cut(s) 64, 193
MmeI TCCRAC 1 cut(s) 773
MnlI CCTC 2 cut(s) 43, 51
Mox20I TGGCCA 1 cut(s) 847
MscI TGGCCA 1 cut(s) 847
MseI TTAA 3 cut(s) 20, 324, 572
MslI CAYNNNNRTG 3 cut(s) 218, 399, 432
Msp20I TGGCCA 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 419, 666
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 978
MvaI CCWGG 2 cut(s) 419, 666
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 3 cut(s) 61, 703, 717
NdeII GATC 5 cut(s) 292, 445, 465, 605, 868
NlaIII CATG 9 cut(s) 223, 287, 335, 398, 437, 569, 641, 710, 846
NlaIV GGNNCC 2 cut(s) 712, 955
NmeAIII GCCGAG 2 cut(s) 73, 910
NmuCI GTSAC 2 cut(s) 626, 802
PaeR7I CTCGAG 1 cut(s) 42
PagI TCATGA 1 cut(s) 637
PctI GAATGC 1 cut(s) 978
PfeI GAWTC 1 cut(s) 670
PkrI GCNGC 2 cut(s) 194, 454
PleI GAGTC 2 cut(s) 64, 193
PpsI GAGTC 2 cut(s) 64, 193
Psp6I CCWGG 2 cut(s) 417, 664
PspGI CCWGG 2 cut(s) 417, 664
PspN4I GGNNCC 2 cut(s) 712, 955
PspPI GGNCC 1 cut(s) 653
PspXI VCTCGAGB 1 cut(s) 42
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 292, 605
RsaI GTAC 2 cut(s) 955, 1035
RsaNI GTAC 2 cut(s) 954, 1034
RseI CAYNNNNRTG 3 cut(s) 218, 399, 432
SaqAI TTAA 3 cut(s) 20, 324, 572
SatI GCNGC 2 cut(s) 193, 453
Sau3AI GATC 5 cut(s) 292, 445, 465, 605, 868
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 2 cut(s) 64, 193
ScrFI CCNGG 2 cut(s) 419, 666
SfaNI GCATC 4 cut(s) 22, 250, 421, 494
SfcI CTRYAG 1 cut(s) 453
Sfr274I CTCGAG 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 3 cut(s) 218, 399, 432
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 6 cut(s) 207, 264, 794, 879, 911, 1029
SsiI CCGC 3 cut(s) 192, 680, 851
SspI AATATT 3 cut(s) 356, 731, 812
SspMI CTAG 3 cut(s) 449, 977, 1026
StyD4I CCNGG 2 cut(s) 417, 664
TaaI ACNGT 3 cut(s) 163, 329, 886
TaiI ACGT 1 cut(s) 122
TaqI TCGA 1 cut(s) 43
TaqII GACCGA 1 cut(s) 996
TasI AATT 6 cut(s) 207, 264, 794, 879, 911, 1029
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 3 cut(s) 20, 324, 572
Tru9I TTAA 3 cut(s) 20, 324, 572
TscAI CASTG 2 cut(s) 355, 721
TseFI GTSAC 2 cut(s) 626, 802
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 2 cut(s) 626, 802
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 2 cut(s) 355, 721
XapI RAATTY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 42
XmiI GTMKAC 2 cut(s) 158, 855
XspI CTAG 3 cut(s) 449, 977, 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.