Rw2G041970

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012526
Physical Location & Seq
Forward (+)
68200716 .. 68204758
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041970.1

Sequence Viewer

Length: 1047 bp
ATGGGGATGAAACAAGCCTTAAAGAGCTTGGATGCGTTTCCTCGAGCCGAGGAGCATTTGTTGCAGAAAACGCAATCTGGGGCTTTTGTTTCTGTTGTTGGTCTGCTCATAATGGCGACGTTGTTTTTGCACGAGCTGAGATATTATCTTTCATCGTATACTGTTCATCAGCAGATGTCTGTGGACTTGAAGCGCGGCGAGACTCTGCCAATTCACATTAACATGACGTTTCCGGCTCTACCTTGTGAAGTCCTGAGTGTGGATGCAATTGATATGTCGGGCAAGCATGAAGTGGATCTTGACACAAATATATGGAAACTTCGCCTTAACAGTCATGGACACATCATCGGCACTGAATATTTGTCTGACCTTGTGGAGAAGGAACATTCTAACAACCATGATGATGGTAAAGAGCATCACCAGGAAAACCATTTACATGGAGCTTTTGATCTAGCTGCAGAAGAACTGATCAAGAAAGTAAAGAAAGCATTAGATGATGGAGAAGGATGCAGGGTTTATGGTGTTTTAGATGTCCAAAGGGTTGCTGGAAACTTTCATATATCAGTTCATGGGTTAAACATTTTCGTTGCACAAATGATTTTTGAAGGATCTAAAAATGTAAATGTAAGTCACATTATTCATGACTTGTCATTTGGCCCAAAATATCCAGGGATTCACAACCCGCTTGACGGAACTGAGCGCATTTTGCATGGCACCAGTGGCACATTCAAATATTATATAAAGATTGTTCCAACTGAGTATAGGTATCTCTCAAAGGAAGTTTTGCCAACTAATCAATTCTCTGTGACAGAATATTATTCCCCAATGAAAGAGTATGATAGGTCATGGCCAGCGGTCTACTTCTTGTATGATCTTTCGCCAATTACTGTAACTATCAAAGAAGAACGACGCAAATTTCTTCATTTCATCACTCGGCTTTGTGCAGTATTGGGTGGTACCTTCGCTTTGACAGGAATGCTAGACCGATGGATGTTCAGGTTTGTTGAAGCATTGACTAAACCAAATGCTAGAATTGTACTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

348

Amino Acids

39.73

Weight (kDa)

6.57

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 2e-27 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 154 - 328 6.5e-46 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22290 AT3G22290
fragaria_vesca FvH4_6g40110 FvH4_6g40110 FvH4_6g40110 FvH4_6g40110
malus_domestica MD09G1135200.v1.1 MD17G1124200.v1.1
prunus_persica Prupe.3G191500_v2.0.a1
pyrus_communis pycom09g05630 pycom17g11560
rosa_chinensis RchiOBHm_Chr2g0154741
rosa_laevigata RLG00000020799
rosa_multiflora Rmu_sc0001027.1_g000004
rosa_roxburghii Rroxscaffold_2G00094140
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2BG521200 Rh2CG495500
rosa_wichuraiana Rw2G041970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 956
AccB1I GGYRCC 2 cut(s) 713, 956
AccI GTMKAC 2 cut(s) 158, 858
AccII CGCG 1 cut(s) 195
AciI CCGC 3 cut(s) 195, 683, 854
AclWI GGATC 2 cut(s) 303, 616
AcoI YGGCCR 1 cut(s) 848
AcsI RAATTY 1 cut(s) 914
AfaI GTAC 2 cut(s) 958, 1038
AfiI CCNNNNNNNGG 2 cut(s) 259, 689
AgsI TTSAA 4 cut(s) 190, 605, 730, 1007
AjnI CCWGG 2 cut(s) 420, 667
AluBI AGCT 4 cut(s) 27, 136, 443, 455
AluI AGCT 4 cut(s) 27, 136, 443, 455
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 2 cut(s) 303, 616
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 2 cut(s) 655, 848
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 914
ArsI GACNNNNNNTTYG 4 cut(s) 109, 141, 635, 667
Asp718I GGTACC 1 cut(s) 956
AspLEI GCGC 2 cut(s) 195, 702
AspS9I GGNCC 1 cut(s) 656
AsuHPI GGTGA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 42
BalI TGGCCA 1 cut(s) 850
BanI GGYRCC 2 cut(s) 713, 956
BauI CACGAG 1 cut(s) 131
BbvI GCAGC 1 cut(s) 442
BccI CCATC 3 cut(s) 398, 491, 981
BciT130I CCWGG 2 cut(s) 422, 669
BclI TGATCA 1 cut(s) 468
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 3 cut(s) 452, 980, 1029
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 2 cut(s) 196, 456
BlsI GCNGC 2 cut(s) 197, 457
Bme1390I CCNGG 2 cut(s) 422, 669
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 2 cut(s) 715, 958
BmrFI CCNGG 2 cut(s) 422, 669
BmsI GCATC 4 cut(s) 22, 253, 424, 497
BsaJI CCNNGG 2 cut(s) 48, 668
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 689
Bse1I ACTGG 1 cut(s) 717
BseBI CCWGG 2 cut(s) 422, 669
BseDI CCNNGG 2 cut(s) 48, 668
BseGI GGATG 5 cut(s) 12, 37, 268, 512, 996
BseLI CCNNNNNNNGG 2 cut(s) 259, 689
BseMII CTCAG 4 cut(s) 128, 245, 687, 747
BseNI ACTGG 1 cut(s) 717
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 657, 850
BshNI GGYRCC 2 cut(s) 713, 956
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 259, 689
BsmAI GTCTC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 981
BsnI GGCC 2 cut(s) 657, 850
BsoBI CYCGRG 1 cut(s) 42
Bsp143I GATC 5 cut(s) 295, 448, 468, 608, 871
BspACI CCGC 3 cut(s) 195, 683, 854
BspANI GGCC 2 cut(s) 657, 850
BspCNI CTCAG 4 cut(s) 129, 246, 688, 748
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 715, 958
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 2 cut(s) 303, 616
BspT107I GGYRCC 2 cut(s) 713, 956
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 2 cut(s) 48, 668
BssMI GATC 5 cut(s) 295, 448, 468, 608, 871
BssNAI GTATAC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 131
Bst1107I GTATAC 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 422, 669
Bst4CI ACNGT 3 cut(s) 163, 332, 889
BstAPI GCANNNNNTGC 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 284, 852
BstDEI CTNAG 4 cut(s) 137, 254, 696, 756
BstF5I GGATG 5 cut(s) 12, 37, 268, 512, 996
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 2 cut(s) 195, 702
BstKTI GATC 5 cut(s) 298, 451, 471, 611, 874
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 5 cut(s) 295, 448, 468, 608, 871
BstMWI GCNNNNNNNGC 4 cut(s) 61, 70, 706, 720
BstNI CCWGG 2 cut(s) 422, 669
BstSCI CCNGG 2 cut(s) 420, 667
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 2 cut(s) 295, 608
BstXI CCANNNNNNTGG 2 cut(s) 404, 437
BstYI RGATCY 2 cut(s) 295, 608
BstZ17I GTATAC 1 cut(s) 159
BsuRI GGCC 2 cut(s) 657, 850
BtsCI GGATG 5 cut(s) 12, 37, 268, 512, 996
BtsIMutI CAGTG 2 cut(s) 351, 724
Cac8I GCNNGC 2 cut(s) 284, 852
CciI TCATGA 1 cut(s) 640
CfoI GCGC 2 cut(s) 195, 702
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 1 cut(s) 918
Csp6I GTAC 2 cut(s) 957, 1037
CviAII CATG 9 cut(s) 223, 287, 335, 398, 437, 569, 641, 710, 846
CviQI GTAC 2 cut(s) 957, 1037
DdeI CTNAG 4 cut(s) 137, 254, 696, 756
DpnI GATC 5 cut(s) 297, 450, 470, 610, 873
DpnII GATC 5 cut(s) 295, 448, 468, 608, 871
EaeI YGGCCR 1 cut(s) 848
Eco88I CYCGRG 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 420, 667
FaeI CATG 9 cut(s) 226, 290, 338, 401, 440, 572, 644, 713, 849
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FatI CATG 9 cut(s) 222, 286, 334, 397, 436, 568, 640, 709, 845
FauI CCCGC 1 cut(s) 690
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 2 cut(s) 158, 858
Fnu4HI GCNGC 2 cut(s) 196, 456
FokI GGATG 5 cut(s) 19, 44, 275, 519, 1003
Fsp4HI GCNGC 2 cut(s) 196, 456
FspBI CTAG 3 cut(s) 452, 980, 1029
GlaI GCGC 2 cut(s) 194, 701
GluI GCNGC 2 cut(s) 196, 456
HaeIII GGCC 2 cut(s) 657, 850
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 918
HhaI GCGC 2 cut(s) 195, 702
Hin1II CATG 9 cut(s) 226, 290, 338, 401, 440, 572, 644, 713, 849
Hin6I GCGC 2 cut(s) 193, 700
HinP1I GCGC 2 cut(s) 193, 700
HinfI GANTC 2 cut(s) 202, 673
HpaII CCGG 1 cut(s) 233
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 3 cut(s) 159, 184, 859
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 4 cut(s) 253, 299, 472, 641
Hpy8I GTNNAC 3 cut(s) 159, 184, 859
Hpy99I CGWCG 2 cut(s) 121, 912
HpyAV CCTTC 4 cut(s) 373, 497, 599, 970
HpyCH4III ACNGT 3 cut(s) 163, 332, 889
HpyCH4IV ACGT 2 cut(s) 119, 227
HpyCH4V TGCA 8 cut(s) 64, 130, 266, 458, 510, 590, 709, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 70, 706, 720
HpyF3I CTNAG 4 cut(s) 137, 254, 696, 756
HpySE526I ACGT 2 cut(s) 119, 227
Hsp92II CATG 9 cut(s) 226, 290, 338, 401, 440, 572, 644, 713, 849
HspAI GCGC 2 cut(s) 193, 700
KpnI GGTACC 1 cut(s) 960
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 5 cut(s) 295, 448, 468, 608, 871
LmnI GCTCC 2 cut(s) 52, 440
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 4 cut(s) 22, 253, 424, 497
MaeI CTAG 3 cut(s) 452, 980, 1029
MaeII ACGT 2 cut(s) 119, 227
MaeIII GTNAC 3 cut(s) 629, 805, 889
MalI GATC 5 cut(s) 297, 450, 470, 610, 873
MboI GATC 5 cut(s) 295, 448, 468, 608, 871
MboII GAAGA 3 cut(s) 473, 911, 914
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 2 cut(s) 295, 608
MlsI TGGCCA 1 cut(s) 850
MluCI AATT 6 cut(s) 210, 267, 797, 882, 914, 1032
MluNI TGGCCA 1 cut(s) 850
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 776
MnlI CCTC 2 cut(s) 43, 51
Mox20I TGGCCA 1 cut(s) 850
MscI TGGCCA 1 cut(s) 850
MseI TTAA 4 cut(s) 20, 219, 327, 575
MslI CAYNNNNRTG 3 cut(s) 221, 402, 435
Msp20I TGGCCA 1 cut(s) 850
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 2 cut(s) 422, 669
MunI CAATTG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 981
MvaI CCWGG 2 cut(s) 422, 669
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 4 cut(s) 61, 70, 706, 720
NdeII GATC 5 cut(s) 295, 448, 468, 608, 871
NlaIII CATG 9 cut(s) 226, 290, 338, 401, 440, 572, 644, 713, 849
NlaIV GGNNCC 2 cut(s) 715, 958
NmeAIII GCCGAG 2 cut(s) 73, 913
NmuCI GTSAC 2 cut(s) 629, 805
PaeR7I CTCGAG 1 cut(s) 42
PagI TCATGA 1 cut(s) 640
PctI GAATGC 1 cut(s) 981
PfeI GAWTC 1 cut(s) 673
PkrI GCNGC 2 cut(s) 197, 457
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 420, 667
PspGI CCWGG 2 cut(s) 420, 667
PspN4I GGNNCC 2 cut(s) 715, 958
PspPI GGNCC 1 cut(s) 656
PspXI VCTCGAGB 1 cut(s) 42
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 2 cut(s) 295, 608
RsaI GTAC 2 cut(s) 958, 1038
RsaNI GTAC 2 cut(s) 957, 1037
RseI CAYNNNNRTG 3 cut(s) 221, 402, 435
SaqAI TTAA 4 cut(s) 20, 219, 327, 575
SatI GCNGC 2 cut(s) 196, 456
Sau3AI GATC 5 cut(s) 295, 448, 468, 608, 871
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 422, 669
SfaNI GCATC 4 cut(s) 22, 253, 424, 497
SfcI CTRYAG 1 cut(s) 456
Sfr274I CTCGAG 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 3 cut(s) 221, 402, 435
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 6 cut(s) 210, 267, 797, 882, 914, 1032
SsiI CCGC 3 cut(s) 195, 683, 854
SspI AATATT 3 cut(s) 359, 734, 815
SspMI CTAG 3 cut(s) 452, 980, 1029
StyD4I CCNGG 2 cut(s) 420, 667
TaaI ACNGT 3 cut(s) 163, 332, 889
TaiI ACGT 2 cut(s) 122, 230
TaqI TCGA 1 cut(s) 43
TaqII GACCGA 1 cut(s) 999
TasI AATT 6 cut(s) 210, 267, 797, 882, 914, 1032
TatI WGTACW 1 cut(s) 1036
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 4 cut(s) 20, 219, 327, 575
Tru9I TTAA 4 cut(s) 20, 219, 327, 575
TscAI CASTG 2 cut(s) 358, 724
TseFI GTSAC 2 cut(s) 629, 805
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 2 cut(s) 629, 805
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 2 cut(s) 358, 724
XapI RAATTY 1 cut(s) 914
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 42
XmiI GTMKAC 2 cut(s) 158, 858
XspI CTAG 3 cut(s) 452, 980, 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.