MD17G1124200.v1.1

Endoplasmic reticulum-Golgi intermediate compartment protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012526
Physical Location & Seq
Reverse (-)
10785908 .. 10790671
4764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1124200.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGGAGTGAAACAAGCGTTAAAGAGCCTGGATGCGTTTCCTCGGGCGGAGGAGCACTTGCTGCAGAAAACACAATCGGGGGCACTCGTTTCTGTTGTTGGGCTGCTTATAATGGCGACATTGTTTGTGCACGAGCTGAGATATTATCTCACTACATATACCGTCCATCAGATGTCTGCAGACTTGAAGCGCGGAGAGACTCTTCCGATTCACATAAATATGACATTTCCAGCTTTACCTTGTGATGTCTTGAGTGTTGATGCAATCGATATGTCAGGCAAGCATGAAGTAGATCTTGACACAAACATATGGAAACTTCGCCTGAACAGTCATGGACACATCATCGGCACTGAATATGTGTCTGACCTTGTGGAAAAGGAGCATTCTCACAAGCACGATGACGGCAAAGATCATCACGACGATAAGGACAAGGAAGTTCATCCAGAAGGAGCTTTTGATCAAGCTGCAGAAGATCTTATCAAGAGGGTAAAGCATGCAATAGCAAATGGAGAAGGATGCCGGGTTTTTGGTGTTTTAGATGTCCAAAGGGTTGCTGGAAACTTTCATATATCAGTTCATGGGTTAAACATTTTTGCTGCGCAAATGATTTTTGAAGGATCTAGAAATGTAAATGTAAGCCACATCATACATGACTTGTCGTTTGGCCCAAAATATCCAGGAATTCACAACCCACTTGATGGGACAGACCGAATTTTGCATGACACAAGTGGCACTTTCAAATATTATATAAAGATTGTTCCAACTGAATATAGGTATATCTCAAAAGAAGTTTTGCCAACCAATCAATTCTCTGTCACAGAATATTTCTCTCCTATGAAGCAGTTTGATAGGACATGGCCAGCTGTATACTTCTTGTATGATCTCTCACCAATTACTGTAACTATCAAAGAAGAACGCCGCAGTTTTCTCCATTTCATCACTCGGCTTTGTGCTGTATTGGGTGGTACCTTTGCGTTGACAGGAATGCTAGATAGATGGATGTACAGGTTTGTTGAAGCATTGACGGCCAAACCACATGCTAGAAGTGTACTGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

353

Amino Acids

40.0

Weight (kDa)

6.51

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 96 5.5e-28 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 137 - 331 3.5e-46 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012526)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22290 AT3G22290
fragaria_vesca FvH4_6g40110 FvH4_6g40110 FvH4_6g40110 FvH4_6g40110
malus_domestica MD09G1135200.v1.1 MD17G1124200.v1.1
prunus_persica Prupe.3G191500_v2.0.a1
pyrus_communis pycom09g05630 pycom17g11560
rosa_chinensis RchiOBHm_Chr2g0154741
rosa_laevigata RLG00000020799
rosa_multiflora Rmu_sc0001027.1_g000004
rosa_roxburghii Rroxscaffold_2G00094140
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2BG521200 Rh2CG495500
rosa_wichuraiana Rw2G041970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 600
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 1 cut(s) 965
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 192
AciI CCGC 3 cut(s) 47, 192, 919
AclWI GGATC 1 cut(s) 625
AcoI YGGCCR 2 cut(s) 857, 1026
AcsI RAATTY 2 cut(s) 681, 711
AfaI GTAC 3 cut(s) 967, 1004, 1050
AfiI CCNNNNNNNGG 1 cut(s) 698
AgsI TTSAA 4 cut(s) 187, 614, 739, 1016
AjnI CCWGG 2 cut(s) 27, 676
AluBI AGCT 5 cut(s) 136, 233, 452, 464, 863
AluI AGCT 5 cut(s) 136, 233, 452, 464, 863
Alw21I GWGCWC 2 cut(s) 57, 132
Alw26I GTCTC 1 cut(s) 191
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 3 cut(s) 664, 857, 1026
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 4 cut(s) 61, 103, 464, 596
ApoI RAATTY 2 cut(s) 681, 711
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
Asp718I GGTACC 1 cut(s) 965
AspLEI GCGC 2 cut(s) 192, 601
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 879
AvaI CYCGRG 1 cut(s) 42
BaeGI GKGCMC 2 cut(s) 85, 132
BalI TGGCCA 1 cut(s) 859
BanI GGYRCC 1 cut(s) 965
BauI CACGAG 1 cut(s) 131
Bbv12I GWGCWC 2 cut(s) 57, 132
BbvI GCAGC 4 cut(s) 48, 90, 451, 583
BccI CCATC 3 cut(s) 174, 692, 990
BceAI ACGGC 2 cut(s) 418, 1041
BciT130I CCWGG 2 cut(s) 29, 678
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 3 cut(s) 621, 989, 1041
BfmI CTRYAG 3 cut(s) 62, 177, 465
BglII AGATCT 2 cut(s) 292, 472
BisI GCNGC 5 cut(s) 62, 104, 465, 597, 919
BlsI GCNGC 5 cut(s) 63, 105, 466, 598, 920
Bme1390I CCNGG 3 cut(s) 29, 521, 678
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 1 cut(s) 967
BmrFI CCNGG 3 cut(s) 29, 521, 678
BmsI GCATC 3 cut(s) 22, 250, 506
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 1 cut(s) 521
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 41
Bsc4I CCNNNNNNNGG 1 cut(s) 698
BseBI CCWGG 2 cut(s) 29, 678
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 4 cut(s) 37, 439, 521, 1005
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 2 cut(s) 85, 132
BseXI GCAGC 4 cut(s) 48, 90, 451, 583
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 3 cut(s) 666, 859, 1028
BshNI GGYRCC 1 cut(s) 965
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 57, 132
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 1 cut(s) 520
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 715
BsmI GAATGC 2 cut(s) 382, 990
BsnI GGCC 3 cut(s) 666, 859, 1028
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 3 cut(s) 57, 85, 132
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 6 cut(s) 292, 409, 457, 472, 617, 880
BspACI CCGC 3 cut(s) 47, 192, 919
BspANI GGCC 3 cut(s) 666, 859, 1028
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 267
BspFNI CGCG 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 967
BspMAI CTGCAG 3 cut(s) 66, 181, 469
BspPI GGATC 1 cut(s) 625
BspT107I GGYRCC 1 cut(s) 965
BsrGI TGTACA 1 cut(s) 1002
BssECI CCNNGG 1 cut(s) 41
BssMI GATC 6 cut(s) 292, 409, 457, 472, 617, 880
BssNAI GTATAC 1 cut(s) 868
BssSI CACGAG 1 cut(s) 131
Bst1107I GTATAC 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 29, 678
Bst4CI ACNGT 3 cut(s) 163, 329, 898
Bst6I CTCTTC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 1002
BstC8I GCNNGC 3 cut(s) 281, 495, 861
BstDEI CTNAG 1 cut(s) 137
BstF5I GGATG 4 cut(s) 37, 439, 521, 1005
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 2 cut(s) 192, 601
BstKTI GATC 6 cut(s) 295, 412, 460, 475, 620, 883
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 6 cut(s) 292, 409, 457, 472, 617, 880
BstMWI GCNNNNNNNGC 2 cut(s) 61, 1025
BstNI CCWGG 2 cut(s) 29, 678
BstNSI RCATGY 2 cut(s) 497, 1040
BstSCI CCNGG 3 cut(s) 27, 519, 676
BstSFI CTRYAG 3 cut(s) 62, 177, 465
BstSLI GKGCMC 2 cut(s) 85, 132
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 4 cut(s) 48, 90, 451, 583
BstX2I RGATCY 3 cut(s) 292, 472, 617
BstYI RGATCY 3 cut(s) 292, 472, 617
BstZ17I GTATAC 1 cut(s) 868
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 3 cut(s) 666, 859, 1028
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 4 cut(s) 37, 439, 521, 1005
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 281, 495, 861
CfoI GCGC 2 cut(s) 192, 601
Cfr13I GGNCC 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 267
Csp6I GTAC 3 cut(s) 966, 1003, 1049
CviAII CATG 8 cut(s) 284, 332, 494, 578, 650, 719, 855, 1037
CviQI GTAC 3 cut(s) 966, 1003, 1049
DdeI CTNAG 1 cut(s) 137
DpnI GATC 6 cut(s) 294, 411, 459, 474, 619, 882
DpnII GATC 6 cut(s) 292, 409, 457, 472, 617, 880
EaeI YGGCCR 2 cut(s) 857, 1026
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EciI GGCGGA 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 42
EcoRI GAATTC 1 cut(s) 681
EcoRII CCWGG 2 cut(s) 27, 676
FaeI CATG 8 cut(s) 287, 335, 497, 581, 653, 722, 858, 1040
FalI AAGNNNNNCTT 4 cut(s) 279, 311, 718, 750
FaqI GGGAC 1 cut(s) 715
FatI CATG 8 cut(s) 283, 331, 493, 577, 649, 718, 854, 1036
FauNDI CATATG 1 cut(s) 308
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 5 cut(s) 62, 104, 465, 597, 919
FokI GGATG 4 cut(s) 44, 426, 528, 1012
Fsp4HI GCNGC 5 cut(s) 62, 104, 465, 597, 919
FspBI CTAG 3 cut(s) 621, 989, 1041
FspI TGCGCA 1 cut(s) 600
GlaI GCGC 2 cut(s) 191, 600
GluI GCNGC 5 cut(s) 62, 104, 465, 597, 919
HaeIII GGCC 3 cut(s) 666, 859, 1028
HapII CCGG 1 cut(s) 520
HhaI GCGC 2 cut(s) 192, 601
Hin1II CATG 8 cut(s) 287, 335, 497, 581, 653, 722, 858, 1040
Hin6I GCGC 2 cut(s) 190, 599
HinP1I GCGC 2 cut(s) 190, 599
HincII GTYRAC 1 cut(s) 978
HindII GTYRAC 1 cut(s) 978
HinfI GANTC 2 cut(s) 199, 208
HpaII CCGG 1 cut(s) 520
HphI GGTGA 1 cut(s) 879
Hpy166II GTNNAC 4 cut(s) 130, 868, 978, 1049
Hpy188I TCNGA 3 cut(s) 171, 207, 364
Hpy188III TCNNGA 6 cut(s) 250, 296, 416, 443, 481, 621
Hpy8I GTNNAC 4 cut(s) 130, 868, 978, 1049
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 3 cut(s) 440, 506, 608
HpyCH4III ACNGT 3 cut(s) 163, 329, 898
HpyCH4V TGCA 7 cut(s) 64, 130, 179, 263, 467, 497, 718
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 1025
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 8 cut(s) 287, 335, 497, 581, 653, 722, 858, 1040
HspAI GCGC 2 cut(s) 190, 599
KpnI GGTACC 1 cut(s) 969
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 6 cut(s) 292, 409, 457, 472, 617, 880
LmnI GCTCC 3 cut(s) 52, 379, 449
Lsp1109I GCAGC 4 cut(s) 48, 90, 451, 583
LweI GCATC 3 cut(s) 22, 250, 506
MaeI CTAG 3 cut(s) 621, 989, 1041
MaeIII GTNAC 2 cut(s) 814, 898
MalI GATC 6 cut(s) 294, 411, 459, 474, 619, 882
MboI GATC 6 cut(s) 292, 409, 457, 472, 617, 880
MboII GAAGA 3 cut(s) 194, 482, 923
MflI RGATCY 3 cut(s) 292, 472, 617
MhlI GDGCHC 3 cut(s) 57, 85, 132
MlsI TGGCCA 1 cut(s) 859
MluCI AATT 4 cut(s) 681, 711, 806, 891
MluNI TGGCCA 1 cut(s) 859
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 785
MnlI CCTC 3 cut(s) 43, 51, 477
Mox20I TGGCCA 1 cut(s) 859
MscI TGGCCA 1 cut(s) 859
MseI TTAA 2 cut(s) 20, 584
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 859
MspA1I CMGCKG 1 cut(s) 863
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 3 cut(s) 29, 521, 678
Mva1269I GAATGC 2 cut(s) 382, 990
MvaI CCWGG 2 cut(s) 29, 678
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 61, 1025
NciI CCSGG 1 cut(s) 521
NdeI CATATG 1 cut(s) 308
NdeII GATC 6 cut(s) 292, 409, 457, 472, 617, 880
NlaIII CATG 8 cut(s) 287, 335, 497, 581, 653, 722, 858, 1040
NlaIV GGNNCC 1 cut(s) 967
NmeAIII GCCGAG 1 cut(s) 922
NmuCI GTSAC 1 cut(s) 814
NsbI TGCGCA 1 cut(s) 600
NspI RCATGY 2 cut(s) 497, 1040
PaeI GCATGC 1 cut(s) 497
PctI GAATGC 2 cut(s) 382, 990
PfeI GAWTC 1 cut(s) 208
PflMI CCANNNNNTGG 1 cut(s) 698
PfoI TCCNGGA 1 cut(s) 676
PkrI GCNGC 5 cut(s) 63, 105, 466, 598, 920
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PsiI TTATAA 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 27, 676
PspGI CCWGG 2 cut(s) 27, 676
PspN4I GGNNCC 1 cut(s) 967
PspPI GGNCC 1 cut(s) 665
PstI CTGCAG 3 cut(s) 66, 181, 469
PsuI RGATCY 3 cut(s) 292, 472, 617
PvuII CAGCTG 1 cut(s) 863
RsaI GTAC 3 cut(s) 967, 1004, 1050
RsaNI GTAC 3 cut(s) 966, 1003, 1049
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 2 cut(s) 20, 584
SatI GCNGC 5 cut(s) 62, 104, 465, 597, 919
Sau3AI GATC 6 cut(s) 292, 409, 457, 472, 617, 880
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 3 cut(s) 29, 521, 678
SduI GDGCHC 3 cut(s) 57, 85, 132
SfaNI GCATC 3 cut(s) 22, 250, 506
SfcI CTRYAG 3 cut(s) 62, 177, 465
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SphI GCATGC 1 cut(s) 497
Sse9I AATT 4 cut(s) 681, 711, 806, 891
SsiI CCGC 3 cut(s) 47, 192, 919
SspI AATATT 2 cut(s) 743, 824
SspMI CTAG 3 cut(s) 621, 989, 1041
StyD4I CCNGG 3 cut(s) 27, 519, 676
TaaI ACNGT 3 cut(s) 163, 329, 898
TaqI TCGA 1 cut(s) 267
TaqII GACCGA 1 cut(s) 723
TasI AATT 4 cut(s) 681, 711, 806, 891
TatI WGTACW 2 cut(s) 1002, 1048
TauI GCSGC 1 cut(s) 921
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 2 cut(s) 20, 584
Tru9I TTAA 2 cut(s) 20, 584
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 4 cut(s) 61, 103, 464, 596
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 6 cut(s) 300, 428, 554, 566, 851, 925
TspRI CASTG 1 cut(s) 355
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 128
XapI RAATTY 2 cut(s) 681, 711
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 2 cut(s) 497, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 867
XspI CTAG 3 cut(s) 621, 989, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.