AT3G22450

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013033
Physical Location & Seq
Forward (+)
7962307 .. 7963516
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22450.1

Sequence Viewer

Length: 936 bp
ATGGCTTTGATGGTTTTGAGAAGAAAGTGTCTCCGTTTGCCAGATTTTATCTCCGTTTTTCGACCTGCACCGCGTTGCTTCTCCTCGAGATTTTCCAACAGTGAAAGTGGTAAAGTTACAGTGACTGGTTTGAGTGGTTGTGGTATAGAAACTGTGGATGATGATGTTTGGCATGTTTCATCATCTTTATCCCAAGCTTGGAGACAGTTCCAAGCAGACACTGCAAAAAACTCTCCCGTTACAATACAAGGAGTAAGAGAAACAATACTCGATGATGAAGATCCCGATCACGATGAAATCGATAACATGAGAATTCGCGGTGATCTTTTCTTCAAGCTCGATCGAGGTTCAAAAGAATTCGAGGAATACAACTATGACTTCCACCGCAAGAATCAACACAAGAACGCTAAAAAGGAGCAAAACCAAGAAGAAGAAACAAAGATAAAGAAGGATAACAAGAAGGAAGTTCGTGACGAATACAAGAAGAGAAATGACTCTTTCACAGGTGAGACGGATCATCCTAATGAAGCTGTGAAGAAGAGAGAAAGAACATTTACGTTTAACCAACTCACGGCGCCATTTCACTACCCGTTTTGCTTAGACATATACATCTCGAAAGAGTCTGTACGAGCATGTGTGATTCACAGGGTGACTAGTAAGGTTGTAACTGTGGCTCATTCGATTTCAAAAGACATGAAATTCGATCTTGGTTCGAGAAGAAACGTTGCAGCTTGCGCTGCGGTTGGGGCAGTTCTTGCTCAAAGAAGTTTGGAAGATGACATACATGATGTTATATACACTCCTAGGAAAGGAGATAAAATTGAAGGTAAGCTTCAGGTCGTGCTTCAAGCTCTTATTGACAATGGTGTTAATGTTAAAGTGAAGCTTAAGCAGAGGAAACTTAAGAAGAAGACTAGTCATCTAATGATGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

311

Amino Acids

35.72

Weight (kDa)

9.13

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 200 - 291 8.2e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22450 AT3G22450
fragaria_vesca FvH4_4g15620
malus_domestica MD13G1193200.v1.1 MD16G1194100.v1.1
prunus_persica Prupe.1G013800_v2.0.a1
pyrus_communis pycom13g16550 pycom16g16390
rosa_chinensis RchiOBHm_Chr4g0416191
rosa_laevigata RLG00000008007
rosa_multiflora Rmu_sc0001145.1_g000002
rosa_roxburghii Rroxscaffold_5G00360050
rosa_rugosa Rorug04G0139800
rosa_samantha Rh4AG201800 Rh4BG199200 Rh4CG214100 Rh4DG199000
rosa_wichuraiana Rw4G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 574
AccII CGCG 2 cut(s) 73, 318
AciI CCGC 4 cut(s) 71, 318, 385, 740
AclI AACGTT 1 cut(s) 723
AclWI GGATC 2 cut(s) 275, 522
AcsI RAATTY 3 cut(s) 312, 356, 698
AcuI CTGAAG 1 cut(s) 818
AcyI GRCGYC 1 cut(s) 575
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 198, 571, 809
AflII CTTAAG 2 cut(s) 887, 902
AgsI TTSAA 5 cut(s) 334, 351, 687, 824, 848
AhlI ACTAGT 2 cut(s) 653, 914
AluBI AGCT 7 cut(s) 197, 337, 530, 731, 832, 851, 886
AluI AGCT 7 cut(s) 197, 337, 530, 731, 832, 851, 886
Alw26I GTCTC 3 cut(s) 35, 196, 503
AlwI GGATC 2 cut(s) 275, 522
AlwNI CAGNNNCTG 2 cut(s) 125, 221
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 1 cut(s) 930
ApeKI GCWGC 2 cut(s) 728, 737
ApoI RAATTY 3 cut(s) 312, 356, 698
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
AspA2I CCTAGG 1 cut(s) 803
AspLEI GCGC 2 cut(s) 577, 737
AsuHPI GGTGA 3 cut(s) 332, 518, 661
AvaI CYCGRG 1 cut(s) 85
AvrII CCTAGG 1 cut(s) 803
BanI GGYRCC 1 cut(s) 574
BarI GAAGNNNNNNTAC 2 cut(s) 765, 797
BbsI GAAGAC 1 cut(s) 917
BbvI GCAGC 2 cut(s) 724, 740
BccI CCATC 2 cut(s) 4, 922
BceAI ACGGC 1 cut(s) 588
BcoDI GTCTC 3 cut(s) 35, 196, 503
BcuI ACTAGT 2 cut(s) 653, 914
BfaI CTAG 4 cut(s) 654, 804, 915, 934
BfoI RGCGCY 1 cut(s) 578
BfrI CTTAAG 2 cut(s) 887, 902
BfuAI ACCTGC 1 cut(s) 73
BisI GCNGC 2 cut(s) 729, 738
BlnI CCTAGG 1 cut(s) 803
BlsI GCNGC 2 cut(s) 730, 739
BmeT110I CYCGRG 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 917
Bsa29I ATCGAT 1 cut(s) 300
BsaBI GATNNNNATC 2 cut(s) 279, 285
BsaHI GRCGYC 1 cut(s) 575
BsaJI CCNNGG 1 cut(s) 803
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 571, 809
Bse1I ACTGG 1 cut(s) 130
Bse8I GATNNNNATC 2 cut(s) 279, 285
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 803
BseGI GGATG 2 cut(s) 163, 517
BseJI GATNNNNATC 2 cut(s) 279, 285
BseLI CCNNNNNNNGG 3 cut(s) 198, 571, 809
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 73
BseXI GCAGC 2 cut(s) 724, 740
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 2 cut(s) 73, 318
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 1 cut(s) 932
BshNI GGYRCC 1 cut(s) 574
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 343
BsiHKCI CYCGRG 1 cut(s) 85
BslI CCNNNNNNNGG 3 cut(s) 198, 571, 809
BsmAI GTCTC 3 cut(s) 35, 196, 503
BsmBI CGTCTC 1 cut(s) 503
BsnI GGCC 1 cut(s) 932
BsoBI CYCGRG 1 cut(s) 85
Bsp143I GATC 6 cut(s) 280, 286, 322, 340, 514, 703
BspACI CCGC 4 cut(s) 71, 318, 385, 740
BspANI GGCC 1 cut(s) 932
BspDI ATCGAT 1 cut(s) 300
BspFNI CGCG 2 cut(s) 73, 318
BspLI GGNNCC 1 cut(s) 576
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 2 cut(s) 275, 522
BspT107I GGYRCC 1 cut(s) 574
BspTI CTTAAG 2 cut(s) 887, 902
BsrI ACTGG 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 803
BssMI GATC 6 cut(s) 280, 286, 322, 340, 514, 703
BssNI GRCGYC 1 cut(s) 575
BssT1I CCWWGG 1 cut(s) 803
Bst4CI ACNGT 5 cut(s) 101, 121, 154, 207, 670
Bst6I CTCTTC 2 cut(s) 479, 533
BstACI GRCGYC 1 cut(s) 575
BstAFI CTTAAG 2 cut(s) 887, 902
BstAPI GCANNNNNTGC 2 cut(s) 221, 755
BstC8I GCNNGC 1 cut(s) 733
BstDEI CTNAG 1 cut(s) 598
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 2 cut(s) 163, 517
BstFNI CGCG 2 cut(s) 73, 318
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 2 cut(s) 577, 737
BstKTI GATC 6 cut(s) 283, 289, 325, 343, 517, 706
BstMAI GTCTC 3 cut(s) 35, 196, 503
BstMBI GATC 6 cut(s) 280, 286, 322, 340, 514, 703
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 5 cut(s) 221, 734, 737, 746, 755
BstNSI RCATGY 2 cut(s) 176, 636
BstUI CGCG 2 cut(s) 73, 318
BstV1I GCAGC 2 cut(s) 724, 740
BstV2I GAAGAC 1 cut(s) 917
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 1 cut(s) 932
BsuTUI ATCGAT 1 cut(s) 300
BtsCI GGATG 2 cut(s) 163, 517
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 3 cut(s) 106, 126, 219
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 733
CaiI CAGNNNCTG 2 cut(s) 125, 221
CfoI GCGC 2 cut(s) 577, 737
ClaI ATCGAT 1 cut(s) 300
Csp6I GTAC 1 cut(s) 626
CviAII CATG 5 cut(s) 173, 307, 633, 694, 785
CviQI GTAC 1 cut(s) 626
DdeI CTNAG 1 cut(s) 598
DinI GGCGCC 1 cut(s) 576
DpnI GATC 6 cut(s) 282, 288, 324, 342, 516, 705
DpnII GATC 6 cut(s) 280, 286, 322, 340, 514, 703
DraIII CACNNNGTG 1 cut(s) 649
Eam1104I CTCTTC 2 cut(s) 479, 533
EarI CTCTTC 2 cut(s) 479, 533
Eco130I CCWWGG 1 cut(s) 803
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 85
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoRI GAATTC 2 cut(s) 312, 356
EcoT14I CCWWGG 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 576
EheI GGCGCC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 803
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 5 cut(s) 176, 310, 636, 697, 788
FalI AAGNNNNNCTT 4 cut(s) 816, 848, 870, 902
FatI CATG 5 cut(s) 172, 306, 632, 693, 784
Fnu4HI GCNGC 2 cut(s) 729, 738
FokI GGATG 2 cut(s) 170, 504
Fsp4HI GCNGC 2 cut(s) 729, 738
FspBI CTAG 4 cut(s) 654, 804, 915, 934
GlaI GCGC 2 cut(s) 576, 736
GluI GCNGC 2 cut(s) 729, 738
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 1 cut(s) 932
HhaI GCGC 2 cut(s) 577, 737
Hin1I GRCGYC 1 cut(s) 575
Hin1II CATG 5 cut(s) 176, 310, 636, 697, 788
Hin6I GCGC 2 cut(s) 575, 735
HinP1I GCGC 2 cut(s) 575, 735
HindIII AAGCTT 3 cut(s) 195, 830, 884
HinfI GANTC 4 cut(s) 391, 494, 620, 640
HphI GGTGA 3 cut(s) 332, 518, 661
Hpy188III TCNNGA 6 cut(s) 87, 284, 290, 470, 613, 714
HpyAV CCTTC 3 cut(s) 442, 454, 818
HpyCH4III ACNGT 5 cut(s) 101, 121, 154, 207, 670
HpyCH4IV ACGT 2 cut(s) 557, 723
HpyCH4V TGCA 3 cut(s) 68, 224, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 221, 734, 737, 746, 755
HpyF3I CTNAG 1 cut(s) 598
HpySE526I ACGT 2 cut(s) 557, 723
Hsp92I GRCGYC 1 cut(s) 575
Hsp92II CATG 5 cut(s) 176, 310, 636, 697, 788
HspAI GCGC 2 cut(s) 575, 735
KasI GGCGCC 1 cut(s) 574
Kzo9I GATC 6 cut(s) 280, 286, 322, 340, 514, 703
LmnI GCTCC 1 cut(s) 415
LpnPI CCDG 6 cut(s) 54, 78, 111, 489, 631, 821
Lsp1109I GCAGC 2 cut(s) 724, 740
MaeI CTAG 4 cut(s) 654, 804, 915, 934
MaeII ACGT 2 cut(s) 557, 723
MaeIII GTNAC 6 cut(s) 115, 121, 238, 470, 649, 664
MalI GATC 6 cut(s) 282, 288, 324, 342, 516, 705
MboI GATC 6 cut(s) 280, 286, 322, 340, 514, 703
MflI RGATCY 1 cut(s) 280
MluCI AATT 4 cut(s) 312, 356, 698, 819
Mly113I GGCGCC 1 cut(s) 575
MlyI GAGTC 2 cut(s) 488, 629
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 94, 338, 355, 888
MseI TTAA 5 cut(s) 561, 870, 876, 888, 903
MslI CAYNNNNRTG 1 cut(s) 522
MspCI CTTAAG 2 cut(s) 887, 902
MvnI CGCG 2 cut(s) 73, 318
MwoI GCNNNNNNNGC 5 cut(s) 221, 734, 737, 746, 755
NarI GGCGCC 1 cut(s) 575
NdeII GATC 6 cut(s) 280, 286, 322, 340, 514, 703
NlaIII CATG 5 cut(s) 176, 310, 636, 697, 788
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 3 cut(s) 121, 470, 649
NspI RCATGY 2 cut(s) 176, 636
PaeR7I CTCGAG 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PfeI GAWTC 2 cut(s) 391, 640
PkrI GCNGC 2 cut(s) 730, 739
Ple19I CGATCG 1 cut(s) 343
PleI GAGTC 2 cut(s) 488, 628
PluTI GGCGCC 1 cut(s) 578
PpsI GAGTC 2 cut(s) 488, 628
Psp1406I AACGTT 1 cut(s) 723
PspN4I GGNNCC 1 cut(s) 576
PstNI CAGNNNCTG 2 cut(s) 125, 221
PsuI RGATCY 1 cut(s) 280
PvuI CGATCG 1 cut(s) 343
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
RseI CAYNNNNRTG 1 cut(s) 522
SaqAI TTAA 5 cut(s) 561, 870, 876, 888, 903
SatI GCNGC 2 cut(s) 729, 738
Sau3AI GATC 6 cut(s) 280, 286, 322, 340, 514, 703
SchI GAGTC 2 cut(s) 488, 629
SfoI GGCGCC 1 cut(s) 576
Sfr274I CTCGAG 1 cut(s) 85
SlaI CTCGAG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 522
SmlI CTYRAG 3 cut(s) 85, 887, 902
SmoI CTYRAG 3 cut(s) 85, 887, 902
SpeI ACTAGT 2 cut(s) 653, 914
Sse9I AATT 4 cut(s) 312, 356, 698, 819
SsiI CCGC 4 cut(s) 71, 318, 385, 740
SspDI GGCGCC 1 cut(s) 574
SspMI CTAG 4 cut(s) 654, 804, 915, 934
StyI CCWWGG 1 cut(s) 803
TaaI ACNGT 5 cut(s) 101, 121, 154, 207, 670
TaiI ACGT 2 cut(s) 560, 726
TasI AATT 4 cut(s) 312, 356, 698, 819
TfiI GAWTC 2 cut(s) 391, 640
Tru1I TTAA 5 cut(s) 561, 870, 876, 888, 903
Tru9I TTAA 5 cut(s) 561, 870, 876, 888, 903
TscAI CASTG 3 cut(s) 106, 126, 226
TseFI GTSAC 3 cut(s) 121, 470, 649
TseI GCWGC 2 cut(s) 728, 737
Tsp45I GTSAC 3 cut(s) 121, 470, 649
TspDTI ATGAA 5 cut(s) 168, 291, 309, 540, 710
TspGWI ACGGA 3 cut(s) 23, 43, 527
TspRI CASTG 3 cut(s) 106, 126, 226
Vha464I CTTAAG 2 cut(s) 887, 902
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 3 cut(s) 312, 356, 698
XceI RCATGY 2 cut(s) 176, 636
XhoI CTCGAG 1 cut(s) 85
XmaJI CCTAGG 1 cut(s) 803
XspI CTAG 4 cut(s) 654, 804, 915, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.