MD16G1194100.v1.1

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013033
Physical Location & Seq
Forward (+)
17218201 .. 17218988
788 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1194100.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCAGGGGTCTGCATTGGACTCAAGTGCGTTATCGGATGAAGTGGTTGATGAGCCAATTGATAGTTGCAGAGCAGCTGTTGAAGGTGATCCGGATTTTGATGACATTGACAACATGAGAATTCACGGCAATCTCTTCTTTAAGCTTGACCGAGATTCCAAAGAGTTTGAAGAGTACAATTTCGACTTCCATCGTAGGAAGAAGTCTTCTAAGCAAAAGGATGACCCAAAGGAAAGTCAAAGGAAGGGAAATGAGTTAAAGGATAGCAAAAGAGACAACCCAAGTTGTGAATTGCCCTCCAGAAGCAAGAAGCAAACTAGAATTGTGGACGATTCTGAGAACTCCGACTTATATAATCAGCTGACAGGTCCTTTCCATGAGCCGTTTTGCCTGGACATTTTTATATCAAAGGCTTCTGTTCGTGCGTGCATCATTCACCGAGTCACCAGTAAGGTTGTCGTTGTGGCACATTCCATATCAAAGGACATGAAATTTGACCTTGGGTCGACTAGGAATGCAGCTGCTTGTGCTGGTGTGGGGGAAATTCTCGCGCAGAGGGCATTAGAAGATGATATCCATGATGTGATTTACACACCAAGGAAAGGGGAGAGATTGGAGGGTAAGCTTGAAATTGTGCTTCAGTCTATCACTGTGCCATGCTCTCCCCTTTCAACAACGAAAACCCTCGGCTTGGGAATTACTCTCTTTCGAGCTAGGAGAGGGGACTGCCATGCTCTCCTGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

249

Amino Acids

27.76

Weight (kDa)

5.85

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 130 - 211 2.2e-07 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22450 AT3G22450
fragaria_vesca FvH4_4g15620
malus_domestica MD13G1193200.v1.1 MD16G1194100.v1.1
prunus_persica Prupe.1G013800_v2.0.a1
pyrus_communis pycom13g16550 pycom16g16390
rosa_chinensis RchiOBHm_Chr4g0416191
rosa_laevigata RLG00000008007
rosa_multiflora Rmu_sc0001145.1_g000002
rosa_roxburghii Rroxscaffold_5G00360050
rosa_rugosa Rorug04G0139800
rosa_samantha Rh4AG201800 Rh4BG199200 Rh4CG214100 Rh4DG199000
rosa_wichuraiana Rw4G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 506
AccII CGCG 1 cut(s) 551
AccIII TCCGGA 1 cut(s) 91
AclWI GGATC 1 cut(s) 83
AcsI RAATTY 3 cut(s) 120, 491, 543
AcuI CTGAAG 1 cut(s) 623
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 2 cut(s) 602, 691
AgsI TTSAA 4 cut(s) 83, 170, 629, 672
AhdI GACNNNNNGTC 1 cut(s) 502
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 6 cut(s) 77, 145, 361, 521, 625, 713
AluI AGCT 6 cut(s) 77, 145, 361, 521, 625, 713
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 74, 518, 521
ApoI RAATTY 3 cut(s) 120, 491, 543
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 3 cut(s) 98, 428, 436
AvaII GGWCC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 86, 508, 530
BccI CCATC 1 cut(s) 198
BceAI ACGGC 2 cut(s) 142, 367
BciT130I CCWGG 1 cut(s) 392
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 3 cut(s) 318, 510, 714
BisI GCNGC 3 cut(s) 75, 519, 522
BlsI GCNGC 3 cut(s) 76, 520, 523
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 368
BmeRI GACNNNNNGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 368
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 283
BpuEI CTTGAG 1 cut(s) 7
BsaJI CCNNGG 3 cut(s) 499, 596, 685
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 602, 691
Bse1I ACTGG 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 91
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 3 cut(s) 499, 596, 685
BseGI GGATG 2 cut(s) 43, 226
BseLI CCNNNNNNNGG 2 cut(s) 602, 691
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 86, 508, 530
Bsh1236I CGCG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 2 cut(s) 602, 691
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 520
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 328
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 1 cut(s) 551
BspPI GGATC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 447
BssECI CCNNGG 3 cut(s) 499, 596, 685
BssMI GATC 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 499, 596
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 2 cut(s) 140, 165
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 210, 336
BstF5I GGATG 2 cut(s) 43, 226
BstFNI CGCG 1 cut(s) 551
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 527, 557
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 390
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 3 cut(s) 86, 508, 530
BstV2I GAAGAC 1 cut(s) 198
BtsCI GGATG 2 cut(s) 43, 226
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 427
CfoI GCGC 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 1 cut(s) 175
CviAII CATG 6 cut(s) 115, 377, 487, 578, 657, 731
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 2 cut(s) 210, 336
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
DriI GACNNNNNGTC 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 140, 165
Eam1105I GACNNNNNGTC 1 cut(s) 502
EarI CTCTTC 2 cut(s) 140, 165
Eco130I CCWWGG 2 cut(s) 499, 596
Eco32I GATATC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 499, 596
ErhI CCWWGG 2 cut(s) 499, 596
FaeI CATG 6 cut(s) 118, 380, 490, 581, 660, 734
FaqI GGGAC 1 cut(s) 737
FatI CATG 6 cut(s) 114, 376, 486, 577, 656, 730
FblI GTMKAC 1 cut(s) 506
Fnu4HI GCNGC 3 cut(s) 75, 519, 522
FokI GGATG 2 cut(s) 50, 233
Fsp4HI GCNGC 3 cut(s) 75, 519, 522
FspBI CTAG 3 cut(s) 318, 510, 714
GlaI GCGC 1 cut(s) 552
GluI GCNGC 3 cut(s) 75, 519, 522
GsuI CTGGAG 1 cut(s) 283
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 553
Hin1II CATG 6 cut(s) 118, 380, 490, 581, 660, 734
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HindIII AAGCTT 2 cut(s) 143, 623
HinfI GANTC 4 cut(s) 20, 155, 332, 441
HpaII CCGG 1 cut(s) 92
HphI GGTGA 3 cut(s) 98, 428, 436
Hpy166II GTNNAC 2 cut(s) 328, 507
Hpy188I TCNGA 3 cut(s) 37, 337, 346
Hpy188III TCNNGA 2 cut(s) 92, 300
Hpy8I GTNNAC 2 cut(s) 328, 507
HpyAV CCTTC 2 cut(s) 77, 238
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4V TGCA 5 cut(s) 4, 14, 69, 429, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 557
HpyF3I CTNAG 2 cut(s) 210, 336
Hsp92II CATG 6 cut(s) 118, 380, 490, 581, 660, 734
HspAI GCGC 1 cut(s) 551
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 1 cut(s) 88
LpnPI CCDG 7 cut(s) 105, 313, 351, 377, 404, 460, 516
Lsp1109I GCAGC 3 cut(s) 86, 508, 530
LweI GCATC 1 cut(s) 438
MaeI CTAG 3 cut(s) 318, 510, 714
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 5 cut(s) 127, 182, 198, 211, 578
MfeI CAATTG 1 cut(s) 57
MluCI AATT 9 cut(s) 57, 120, 178, 290, 321, 491, 543, 630, 696
MlyI GAGTC 2 cut(s) 14, 450
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 5 cut(s) 307, 549, 610, 695, 713
MroI TCCGGA 1 cut(s) 91
MseI TTAA 2 cut(s) 141, 257
MspA1I CMGCKG 3 cut(s) 77, 361, 521
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 551
MwoI GCNNNNNNNGC 2 cut(s) 527, 557
NdeII GATC 1 cut(s) 88
NlaIII CATG 6 cut(s) 118, 380, 490, 581, 660, 734
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 442
PctI GAATGC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 155, 332
PkrI GCNGC 3 cut(s) 76, 520, 523
PleI GAGTC 2 cut(s) 14, 449
PpsI GAGTC 2 cut(s) 14, 449
PpuMI RGGWCCY 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspPI GGNCC 1 cut(s) 368
PspPPI RGGWCCY 1 cut(s) 368
PvuII CAGCTG 3 cut(s) 77, 361, 521
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SalI GTCGAC 1 cut(s) 505
SaqAI TTAA 2 cut(s) 141, 257
SatI GCNGC 3 cut(s) 75, 519, 522
Sau3AI GATC 1 cut(s) 88
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 2 cut(s) 14, 450
ScrFI CCNGG 1 cut(s) 392
SfaNI GCATC 1 cut(s) 438
SinI GGWCC 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 9 cut(s) 57, 120, 178, 290, 321, 491, 543, 630, 696
SspMI CTAG 3 cut(s) 318, 510, 714
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 2 cut(s) 499, 596
TaaI ACNGT 1 cut(s) 652
TaqI TCGA 3 cut(s) 183, 506, 709
TaqII GACCGA 1 cut(s) 165
TasI AATT 9 cut(s) 57, 120, 178, 290, 321, 491, 543, 630, 696
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 2 cut(s) 155, 332
Tru1I TTAA 2 cut(s) 141, 257
Tru9I TTAA 2 cut(s) 141, 257
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 3 cut(s) 74, 518, 521
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 2 cut(s) 54, 503
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 3 cut(s) 120, 491, 543
XmiI GTMKAC 1 cut(s) 506
XspI CTAG 3 cut(s) 318, 510, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.