Rw4G017060

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013033
Physical Location & Seq
Reverse (-)
38590514 .. 38592668
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017060.1

Sequence Viewer

Length: 1017 bp
ATGTTGTTGAGGAGAGGAATCAAATTCGCTTCGGCAGCATTTCCACATTTCCTTTCAAAGTCTCCTACTTGTTGCCGCAAAGCCACCACCACGGTTCCCATTTTCGATCATTCGGCATACTACTCTAGTGGCTTTCGCCTCTCTCCTTTGTTCCCCTCCGAACCCAAACCTCAGTCGGATAGTTATGATATTGAGCTTGTTGACGATGATGCATGGCGGGTTTCCTCAGGTTTGGCGCTAGCGGATCAGGGGACTGCATTGGAGGAACAAAGTTGCTTATCGGATGAAGCGGTTGATGAACCTGTTGATAGTTGCTCTCTACCTCTTGAGGGTGACCCGGATTTCGATGACATTGACAACATGAGAGTTCGGGGTAAACTGTTCTATAAAATCGACCGCGATTCCAAAGAGTTTGAAGAGTATAGTTTCGACTTCCATCGTAGGAACAAGTCTTCTAAGAAGAAGGATGATCCAAAGGAGAAGACACAGAAGGTTGGTAGTTTGAAGGAAAGCAAAAGAGACAACCCAATTCCTAAGTTTTCCTCCAGAAGTGAGAAACAGTGTAGAGTTGTTGTGGACAACTCTCGGCTAGGTGAAACGGGCGCTACTTGTGTTGAGAAGAAGAAGCTGAGAACTCCGACTTATAATCAGCTCACTGGTCCTTTCCACGAGCCGTTTTGCCTTGATGTTTACATCTCCAAGGCTTCAGTTCGTGCCTGCATTGTTCACAGGTTCACCAGTAAGGTTGTTGCCGTGGCACATTCCATATCGAAGGACATGAAGTTTGACCTTGGTTCAACTAGGAATGCAGCTGCTTGTGCTGCTGTTGGGGAAGTTCTGGCTCAGAGGGCATTGGGAGATGACATCCATGATGTGATTTACACACCAAGGAAAGGGGAAAGGTTGGAGGGCAAGCTTCAAATTGTGGTTCAGTCTCTAATTGATAACGGCATTAATGTGAAGGTGAAGATCAAACAAAGGGCGCGGAAGAGACCAACTTCCTTTTCCACAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

338

Amino Acids

37.82

Weight (kDa)

8.82

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 227 - 319 1.8e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22450 AT3G22450
fragaria_vesca FvH4_4g15620
malus_domestica MD13G1193200.v1.1 MD16G1194100.v1.1
prunus_persica Prupe.1G013800_v2.0.a1
pyrus_communis pycom13g16550 pycom16g16390
rosa_chinensis RchiOBHm_Chr4g0416191
rosa_laevigata RLG00000008007
rosa_multiflora Rmu_sc0001145.1_g000002
rosa_roxburghii Rroxscaffold_5G00360050
rosa_rugosa Rorug04G0139800
rosa_samantha Rh4AG201800 Rh4BG199200 Rh4CG214100 Rh4DG199000
rosa_wichuraiana Rw4G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AccII CGCG 2 cut(s) 399, 985
AciI CCGC 6 cut(s) 76, 217, 242, 290, 397, 985
AclWI GGATC 2 cut(s) 252, 464
AcsI RAATTY 1 cut(s) 23
AcuI CTGAAG 1 cut(s) 690
AfiI CCNNNNNNNGG 2 cut(s) 329, 893
AgsI TTSAA 5 cut(s) 57, 416, 505, 798, 920
AjuI GAANNNNNNNTTGG 1 cut(s) 988
AluBI AGCT 6 cut(s) 196, 628, 652, 812, 916, 1013
AluI AGCT 6 cut(s) 196, 628, 652, 812, 916, 1013
Alw26I GTCTC 4 cut(s) 66, 513, 939, 985
AlwI GGATC 2 cut(s) 252, 464
ApeKI GCWGC 4 cut(s) 35, 809, 812, 821
ApoI RAATTY 1 cut(s) 23
ArsI GACNNNNNNTTYG 4 cut(s) 326, 358, 767, 799
AseI ATTAAT 1 cut(s) 954
AspLEI GCGC 3 cut(s) 238, 605, 985
AspS9I GGNCC 1 cut(s) 659
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 4 cut(s) 344, 605, 727, 976
AsuNHI GCTAGC 1 cut(s) 238
AvaII GGWCC 1 cut(s) 659
AxyI CCTNAGG 1 cut(s) 226
BauI CACGAG 1 cut(s) 668
BbsI GAAGAC 2 cut(s) 444, 488
BbvI GCAGC 4 cut(s) 47, 799, 808, 821
BccI CCATC 1 cut(s) 444
BceAI ACGGC 3 cut(s) 658, 737, 964
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 4 cut(s) 66, 513, 939, 985
BfaI CTAG 4 cut(s) 126, 239, 590, 801
BfoI RGCGCY 2 cut(s) 239, 606
BisI GCNGC 5 cut(s) 36, 76, 810, 813, 822
BlsI GCNGC 5 cut(s) 37, 77, 811, 814, 823
Bme1390I CCNGG 1 cut(s) 338
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 199
BmtI GCTAGC 1 cut(s) 242
BpiI GAAGAC 2 cut(s) 444, 488
BpmI CTGGAG 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 338
BsaI GGTCTC 1 cut(s) 985
BsaJI CCNNGG 5 cut(s) 90, 699, 753, 790, 887
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 893
Bse1I ACTGG 2 cut(s) 661, 738
Bse21I CCTNAGG 1 cut(s) 226
BseDI CCNNGG 5 cut(s) 90, 699, 753, 790, 887
BseGI GGATG 3 cut(s) 289, 472, 864
BseLI CCNNNNNNNGG 2 cut(s) 329, 893
BseMII CTCAG 4 cut(s) 185, 240, 620, 857
BseNI ACTGG 2 cut(s) 661, 738
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 4 cut(s) 47, 799, 808, 821
Bsh1236I CGCG 2 cut(s) 399, 985
Bsh1285I CGRYCG 1 cut(s) 397
BsiEI CGRYCG 1 cut(s) 397
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 329, 893
BsmAI GTCTC 4 cut(s) 66, 513, 939, 985
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 811
Bso31I GGTCTC 1 cut(s) 985
Bsp143I GATC 4 cut(s) 106, 244, 469, 969
BspACI CCGC 6 cut(s) 76, 217, 242, 290, 397, 985
BspCNI CTCAG 4 cut(s) 184, 239, 621, 856
BspFNI CGCG 2 cut(s) 399, 985
BspLI GGNNCC 1 cut(s) 96
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 2 cut(s) 252, 464
BspTNI GGTCTC 1 cut(s) 985
BsrI ACTGG 2 cut(s) 661, 738
BssECI CCNNGG 5 cut(s) 90, 699, 753, 790, 887
BssMI GATC 4 cut(s) 106, 244, 469, 969
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 3 cut(s) 699, 790, 887
Bst2BI CACGAG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 94, 381, 561
Bst6I CTCTTC 2 cut(s) 411, 983
BstC8I GCNNGC 3 cut(s) 240, 718, 914
BstDEI CTNAG 7 cut(s) 171, 226, 456, 534, 629, 843, 1014
BstDSI CCRYGG 2 cut(s) 90, 753
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 3 cut(s) 289, 472, 864
BstFNI CGCG 2 cut(s) 399, 985
BstH2I RGCGCY 2 cut(s) 239, 606
BstHHI GCGC 3 cut(s) 238, 605, 985
BstKTI GATC 4 cut(s) 109, 247, 472, 972
BstMAI GTCTC 4 cut(s) 66, 513, 939, 985
BstMBI GATC 4 cut(s) 106, 244, 469, 969
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 4 cut(s) 35, 818, 821, 848
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 336
BstUI CGCG 2 cut(s) 399, 985
BstV1I GCAGC 4 cut(s) 47, 799, 808, 821
BstV2I GAAGAC 2 cut(s) 444, 488
Bsu36I CCTNAGG 1 cut(s) 226
BtgI CCRYGG 2 cut(s) 90, 753
BtsCI GGATG 3 cut(s) 289, 472, 864
BtsIMutI CAGTG 2 cut(s) 566, 654
Cac8I GCNNGC 3 cut(s) 240, 718, 914
CfoI GCGC 3 cut(s) 238, 605, 985
Cfr13I GGNCC 1 cut(s) 659
CviAII CATG 4 cut(s) 213, 361, 778, 869
DdeI CTNAG 7 cut(s) 171, 226, 456, 534, 629, 843, 1014
DpnI GATC 4 cut(s) 108, 246, 471, 971
DpnII GATC 4 cut(s) 106, 244, 469, 969
Eam1104I CTCTTC 2 cut(s) 411, 983
EarI CTCTTC 2 cut(s) 411, 983
Eco130I CCWWGG 3 cut(s) 699, 790, 887
Eco31I GGTCTC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 659
Eco57I CTGAAG 1 cut(s) 690
Eco81I CCTNAGG 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 332
EcoT14I CCWWGG 3 cut(s) 699, 790, 887
EcoT22I ATGCAT 1 cut(s) 214
ErhI CCWWGG 3 cut(s) 699, 790, 887
FaeI CATG 4 cut(s) 216, 364, 781, 872
FaqI GGGAC 1 cut(s) 265
FatI CATG 4 cut(s) 212, 360, 777, 868
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 36, 76, 810, 813, 822
FokI GGATG 3 cut(s) 296, 479, 851
Fsp4HI GCNGC 5 cut(s) 36, 76, 810, 813, 822
FspBI CTAG 4 cut(s) 126, 239, 590, 801
GlaI GCGC 3 cut(s) 237, 604, 984
GluI GCNGC 5 cut(s) 36, 76, 810, 813, 822
GsuI CTGGAG 1 cut(s) 529
HaeII RGCGCY 2 cut(s) 239, 606
HapII CCGG 1 cut(s) 338
HhaI GCGC 3 cut(s) 238, 605, 985
Hin1II CATG 4 cut(s) 216, 364, 781, 872
Hin6I GCGC 3 cut(s) 236, 603, 983
HinP1I GCGC 3 cut(s) 236, 603, 983
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 2 cut(s) 18, 401
HpaII CCGG 1 cut(s) 338
HphI GGTGA 4 cut(s) 344, 605, 727, 976
Hpy166II GTNNAC 6 cut(s) 202, 377, 577, 691, 727, 735
Hpy188I TCNGA 5 cut(s) 160, 178, 283, 639, 846
Hpy188III TCNNGA 2 cut(s) 326, 546
Hpy8I GTNNAC 6 cut(s) 202, 377, 577, 691, 727, 735
HpyAV CCTTC 5 cut(s) 457, 484, 499, 766, 955
HpyCH4III ACNGT 3 cut(s) 94, 381, 561
HpyCH4V TGCA 4 cut(s) 212, 257, 720, 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 818, 821, 848
HpyF3I CTNAG 7 cut(s) 171, 226, 456, 534, 629, 843, 1014
Hsp92II CATG 4 cut(s) 216, 364, 781, 872
HspAI GCGC 3 cut(s) 236, 603, 983
Kzo9I GATC 4 cut(s) 106, 244, 469, 969
Lsp1109I GCAGC 4 cut(s) 47, 799, 808, 821
LweI GCATC 1 cut(s) 199
MaeI CTAG 4 cut(s) 126, 239, 590, 801
MaeIII GTNAC 1 cut(s) 332
MalI GATC 4 cut(s) 108, 246, 471, 971
MboI GATC 4 cut(s) 106, 244, 469, 969
MboII GAAGA 8 cut(s) 428, 444, 472, 493, 631, 634, 979, 1000
MluCI AATT 4 cut(s) 23, 528, 921, 939
MmeI TCCRAC 3 cut(s) 156, 662, 885
Mph1103I ATGCAT 1 cut(s) 214
MseI TTAA 1 cut(s) 954
MslI CAYNNNNRTG 1 cut(s) 956
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 338
Mva1269I GAATGC 1 cut(s) 811
MvnI CGCG 2 cut(s) 399, 985
MwoI GCNNNNNNNGC 4 cut(s) 35, 818, 821, 848
NciI CCSGG 1 cut(s) 338
NdeII GATC 4 cut(s) 106, 244, 469, 969
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 4 cut(s) 216, 364, 781, 872
NlaIV GGNNCC 1 cut(s) 96
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 332
NsiI ATGCAT 1 cut(s) 214
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 2 cut(s) 18, 401
PkrI GCNGC 5 cut(s) 37, 77, 811, 814, 823
PshBI ATTAAT 1 cut(s) 954
PsiI TTATAA 1 cut(s) 645
PspEI GGTNACC 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 659
PvuII CAGCTG 1 cut(s) 812
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 1 cut(s) 954
SatI GCNGC 5 cut(s) 36, 76, 810, 813, 822
Sau3AI GATC 4 cut(s) 106, 244, 469, 969
Sau96I GGNCC 1 cut(s) 659
ScrFI CCNGG 1 cut(s) 338
SfaNI GCATC 1 cut(s) 199
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
Sse9I AATT 4 cut(s) 23, 528, 921, 939
SsiI CCGC 6 cut(s) 76, 217, 242, 290, 397, 985
SspMI CTAG 4 cut(s) 126, 239, 590, 801
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 3 cut(s) 699, 790, 887
TaaI ACNGT 3 cut(s) 94, 381, 561
TaqI TCGA 5 cut(s) 105, 345, 393, 429, 770
TasI AATT 4 cut(s) 23, 528, 921, 939
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 2 cut(s) 18, 401
Tru1I TTAA 1 cut(s) 954
Tru9I TTAA 1 cut(s) 954
TscAI CASTG 2 cut(s) 566, 661
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 4 cut(s) 35, 809, 812, 821
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 3 cut(s) 300, 312, 794
TspRI CASTG 2 cut(s) 566, 661
VpaK11BI GGWCC 1 cut(s) 659
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 1 cut(s) 23
XspI CTAG 4 cut(s) 126, 239, 590, 801
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.