pycom13g16550

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013033
Physical Location & Seq
Forward (+)
12363430 .. 12364197
768 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16550.1

Sequence Viewer

Length: 768 bp
ATGCAGGGGTCTGCATTGGACTCAAGTTCGTTATCTGATGAAGTGGTTGATGAGCCAATTGATACTTGCTCAGTACCTGTTGAAGGTGACCCGGACTTTGATGACATTGACAACATGAGAATTCGTGGCCATCTGTTCTTTAAACTCGACCGCGATTCCAAAGAGTTTGAGGAGTATAATTTTGACTTCCATCGCAGGAAGAAGTCTTCTAAACAAAAGGATGATCCAAAGGAAAGTAAAAGGAAGGATAATGAGTTAAGGGACAGCAAAAGAGACAACCTAAGTCTTGACTTGCCCTCCAGAAGCGAGAAACAATCTAGAATTGTGAACGATTCTCCACTTGATGAATTGGGCACTATTTCTGTTGGGAAGAAGAAGCTGAGGAGTCCGACCTATAATCAGCTAACGGGTCCTTTCCATGAGCCATTTTGCCTGGACATTTTCATATCAAAGGCTTCTGTTCGTGCGTGCATCATTCACCGAGTGACCAGTAAGGTTGTTGTTGTGGCACATTCCATATCGAAGGACATGAAGTTTGACCTTTGTTCGACTAGGAATGCAGCTGCTTGTGCTGCTGTGGGGGAAATTCTCGCGCAGAGGGCATTGGATGATGATATCCATGATGTGATTTACACACCTAGGAAGGGGGATAAATTGGAGGGTAAGCTTCAAATTGTGCTTCAGTCTGTCATTGATAGTGGGATTAATGTGAAGGTGAAGTTGAAGCAAATAAACAGGAAGAAACGTAGTTCCTCGTATGCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

256

Amino Acids

28.79

Weight (kDa)

8.18

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 144 - 236 6.4e-09 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22450 AT3G22450
fragaria_vesca FvH4_4g15620
malus_domestica MD13G1193200.v1.1 MD16G1194100.v1.1
prunus_persica Prupe.1G013800_v2.0.a1
pyrus_communis pycom13g16550 pycom16g16390
rosa_chinensis RchiOBHm_Chr4g0416191
rosa_laevigata RLG00000008007
rosa_multiflora Rmu_sc0001145.1_g000002
rosa_roxburghii Rroxscaffold_5G00360050
rosa_rugosa Rorug04G0139800
rosa_samantha Rh4AG201800 Rh4BG199200 Rh4CG214100 Rh4DG199000
rosa_wichuraiana Rw4G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 153, 593
AciI CCGC 1 cut(s) 151
AclWI GGATC 1 cut(s) 218
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 2 cut(s) 120, 585
AcuI CTGAAG 1 cut(s) 665
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 83, 644
AgsI TTSAA 3 cut(s) 83, 671, 724
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 4 cut(s) 379, 403, 563, 667
AluI AGCT 4 cut(s) 379, 403, 563, 667
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 1 cut(s) 218
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 3 cut(s) 560, 563, 572
ApoI RAATTY 2 cut(s) 120, 585
ArsI GACNNNNNNTTYG 4 cut(s) 80, 112, 518, 550
AseI ATTAAT 1 cut(s) 705
AspA2I CCTAGG 1 cut(s) 638
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 98, 470, 727
AvaII GGWCC 1 cut(s) 410
AvrII CCTAGG 1 cut(s) 638
BaeGI GKGCMC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 380
BbvI GCAGC 3 cut(s) 550, 559, 572
BccI CCATC 2 cut(s) 138, 198
BciT130I CCWGG 1 cut(s) 434
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 3 cut(s) 318, 552, 639
BisI GCNGC 3 cut(s) 561, 564, 573
BlnI CCTAGG 1 cut(s) 638
BlsI GCNGC 3 cut(s) 562, 565, 574
Bme1390I CCNGG 2 cut(s) 92, 434
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 1 cut(s) 411
BmrFI CCNGG 2 cut(s) 92, 434
BmsI GCATC 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 283
Bpu10I CCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 7
BpuMI CCSGG 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 644
Bse1I ACTGG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 638
BseGI GGATG 2 cut(s) 226, 613
BseLI CCNNNNNNNGG 2 cut(s) 83, 644
BseMII CTCAG 2 cut(s) 84, 371
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 185, 397
BseSI GKGCMC 1 cut(s) 356
BseXI GCAGC 3 cut(s) 550, 559, 572
Bsh1236I CGCG 2 cut(s) 153, 593
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 2 cut(s) 83, 644
BsmAI GTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 1 cut(s) 223
BspACI CCGC 1 cut(s) 151
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 2 cut(s) 83, 372
BspFNI CGCG 2 cut(s) 153, 593
BspLI GGNNCC 1 cut(s) 411
BspPI GGATC 1 cut(s) 218
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 1 cut(s) 638
BssMI GATC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 638
Bst2UI CCWGG 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 4 cut(s) 70, 281, 380, 765
BstEII GGTNACC 1 cut(s) 86
BstENI CCTNNNNNAGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 226, 613
BstFNI CGCG 2 cut(s) 153, 593
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 1 cut(s) 226
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 1 cut(s) 223
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 3 cut(s) 569, 572, 599
BstNI CCWGG 1 cut(s) 434
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 2 cut(s) 90, 432
BstSLI GKGCMC 1 cut(s) 356
BstUI CGCG 2 cut(s) 153, 593
BstV1I GCAGC 3 cut(s) 550, 559, 572
BstV2I GAAGAC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 176
BtsCI GGATG 2 cut(s) 226, 613
Cac8I GCNNGC 1 cut(s) 469
CaiI CAGNNNCTG 1 cut(s) 77
CfoI GCGC 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 74
CviAII CATG 4 cut(s) 115, 419, 529, 620
CviJI RGCY 8 cut(s) 55, 129, 379, 403, 424, 455, 563, 667
CviKI_1 RGCY 8 cut(s) 55, 129, 379, 403, 424, 455, 563, 667
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 4 cut(s) 70, 281, 380, 765
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
DraI TTTAAA 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 638
Eco32I GATATC 1 cut(s) 616
Eco47I GGWCC 1 cut(s) 410
Eco57I CTGAAG 1 cut(s) 665
Eco91I GGTNACC 1 cut(s) 86
EcoNI CCTNNNNNAGG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 432
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 638
ErhI CCWWGG 1 cut(s) 638
FaeI CATG 4 cut(s) 118, 422, 532, 623
FaiI YATR 9 cut(s) 116, 177, 396, 420, 446, 518, 530, 621, 759
FaqI GGGAC 1 cut(s) 275
FatI CATG 4 cut(s) 114, 418, 528, 619
Fnu4HI GCNGC 3 cut(s) 561, 564, 573
FokI GGATG 2 cut(s) 233, 620
Fsp4HI GCNGC 3 cut(s) 561, 564, 573
FspBI CTAG 3 cut(s) 318, 552, 639
GlaI GCGC 1 cut(s) 594
GluI GCNGC 3 cut(s) 561, 564, 573
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 595
Hin1II CATG 4 cut(s) 118, 422, 532, 623
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 4 cut(s) 20, 155, 332, 385
HpaII CCGG 1 cut(s) 92
HphI GGTGA 3 cut(s) 98, 470, 727
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 2 cut(s) 37, 390
Hpy188III TCNNGA 3 cut(s) 287, 300, 318
Hpy8I GTNNAC 1 cut(s) 328
HpyAV CCTTC 5 cut(s) 77, 238, 517, 637, 706
HpyCH4IV ACGT 1 cut(s) 745
HpyCH4V TGCA 5 cut(s) 4, 14, 471, 560, 761
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 572, 599
HpyF3I CTNAG 4 cut(s) 70, 281, 380, 765
HpySE526I ACGT 1 cut(s) 745
Hsp92II CATG 4 cut(s) 118, 422, 532, 623
HspAI GCGC 1 cut(s) 593
Kzo9I GATC 1 cut(s) 223
LpnPI CCDG 8 cut(s) 90, 105, 181, 313, 419, 446, 502, 721
Lsp1109I GCAGC 3 cut(s) 550, 559, 572
LweI GCATC 1 cut(s) 480
MaeI CTAG 3 cut(s) 318, 552, 639
MaeII ACGT 1 cut(s) 745
MaeIII GTNAC 2 cut(s) 86, 484
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MboII GAAGA 5 cut(s) 198, 211, 382, 385, 751
MfeI CAATTG 1 cut(s) 57
MhlI GDGCHC 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 8 cut(s) 57, 120, 178, 321, 347, 585, 653, 672
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 2 cut(s) 14, 394
MmeI TCCRAC 1 cut(s) 413
MnlI CCTC 6 cut(s) 163, 307, 375, 591, 652, 763
Mox20I TGGCCA 1 cut(s) 129
MscI TGGCCA 1 cut(s) 129
MseI TTAA 3 cut(s) 141, 257, 705
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 2 cut(s) 92, 434
MunI CAATTG 1 cut(s) 57
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 2 cut(s) 153, 593
MwoI GCNNNNNNNGC 3 cut(s) 569, 572, 599
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 223
NlaIII CATG 4 cut(s) 118, 422, 532, 623
NlaIV GGNNCC 1 cut(s) 411
NmuCI GTSAC 2 cut(s) 86, 484
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 2 cut(s) 155, 332
PkrI GCNGC 3 cut(s) 562, 565, 574
PleI GAGTC 2 cut(s) 14, 393
PpsI GAGTC 2 cut(s) 14, 393
PpuMI RGGWCCY 1 cut(s) 410
PshBI ATTAAT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 432
PspEI GGTNACC 1 cut(s) 86
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 411
PspPI GGNCC 1 cut(s) 410
PspPPI RGGWCCY 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 141, 257, 705
SatI GCNGC 3 cut(s) 561, 564, 573
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 2 cut(s) 14, 394
ScrFI CCNGG 2 cut(s) 92, 434
SduI GDGCHC 1 cut(s) 356
SfaNI GCATC 1 cut(s) 480
SinI GGWCC 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
Sse9I AATT 8 cut(s) 57, 120, 178, 321, 347, 585, 653, 672
SsiI CCGC 1 cut(s) 151
SspMI CTAG 3 cut(s) 318, 552, 639
StyD4I CCNGG 2 cut(s) 90, 432
StyI CCWWGG 1 cut(s) 638
TaiI ACGT 1 cut(s) 748
TaqI TCGA 3 cut(s) 147, 521, 548
TasI AATT 8 cut(s) 57, 120, 178, 321, 347, 585, 653, 672
TfiI GAWTC 2 cut(s) 155, 332
Tru1I TTAA 3 cut(s) 141, 257, 705
Tru9I TTAA 3 cut(s) 141, 257, 705
TseFI GTSAC 2 cut(s) 86, 484
TseI GCWGC 3 cut(s) 560, 563, 572
Tsp45I GTSAC 2 cut(s) 86, 484
TspDTI ATGAA 4 cut(s) 54, 360, 433, 545
VpaK11BI GGWCC 1 cut(s) 410
VspI ATTAAT 1 cut(s) 705
XagI CCTNNNNNAGG 1 cut(s) 81
XapI RAATTY 2 cut(s) 120, 585
XbaI TCTAGA 1 cut(s) 317
XmaJI CCTAGG 1 cut(s) 638
XspI CTAG 3 cut(s) 318, 552, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.