MD13G1193200.v1.1

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013033
Physical Location & Seq
Forward (+)
16732682 .. 16733722
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1193200.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGTTCTCAAGGAGAGGAATCGCATTTGCTTCGGCACTGTCTCCGCATTTCCTTTCAAGGGCTGCTCGTAACTTTTGCTGCAAGCCAACCCCCACAACCATACTTCCCGTAGCAGATCATTCCGGGAAGGTTGCCAATTACTCTAGTAACTTTCATCTTTCTCCTTTGTTTTCCACCGATGATTGTAAACCTCATTTGGACGGTTATGATATTGAGCTTGTTGATCATGAGGCGTGGCAAGTTTCATCCGGTTTGGCATTGGCAGGGGGAGGAATGCAGGGGTCTGCATTGGACTCAAATTCGTTATCAGATGAAGTGGTTGATGAGCCAATTGATACTTGCTCAGTACCTGTTGAAGGTGACCCGGACTTTGATGACATTGACAACATGAGAATTCGTGGCCATCTGTTCTTTAAACTTGACCGCGATTCCAAAGAGTTCGAGGAGTATAATTTTGACTTCCATCGCAGGAAGAAGTCTTCTAAGCAAAAGGATGATCCAAAGGAAAGTAAAAGGAAGGATAATGAGTTAAAGGACAGCAAAAGAGGCAACCCAAGTCTTGACTTGCCCTCCAGAAGCGAGAAACAATCTAGAATTGTGAACGATTCTCCGCTTGATGAATTGGACACTACTTCTGTTGGGAAGAAGAAGCTGAGGAGTCCGACCTATAATCAGCTAACAGGTCCCTTCCATGAGCCATTTTGCCTGGACATTTTCATATCAAAGGCTTCTGTTCGTGCGTGCATCATTCACCGCGTGACCAGTAAGGTGGTTGTTGTGGCACATTCCATATCGAAGGACATGAAGTTTGACCTTTGTTCGACTAGGAATGCAGCTGCTTGTGCTGCTGTGGGGGAAATTCTCGCGCAGAGGGCATTGGATGATGATATCCATGATGTGATTTACACACCTAGGAAAGGGGATAAATTGGAGGGTAAGCTTCAAATTGTGCTTCAGTCTGTCATTGATAGTGGGATTAATGTGAAGGTGAAGTTGAGGCAAATAAACAGGAAGAAACGTAGTTCCTCGTATGCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

347

Amino Acids

38.55

Weight (kDa)

7.62

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 235 - 327 1.2e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22450 AT3G22450
fragaria_vesca FvH4_4g15620
malus_domestica MD13G1193200.v1.1 MD16G1194100.v1.1
prunus_persica Prupe.1G013800_v2.0.a1
pyrus_communis pycom13g16550 pycom16g16390
rosa_chinensis RchiOBHm_Chr4g0416191
rosa_laevigata RLG00000008007
rosa_multiflora Rmu_sc0001145.1_g000002
rosa_roxburghii Rroxscaffold_5G00360050
rosa_rugosa Rorug04G0139800
rosa_samantha Rh4AG201800 Rh4BG199200 Rh4CG214100 Rh4DG199000
rosa_wichuraiana Rw4G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 426, 756, 866
AciI CCGC 4 cut(s) 44, 424, 611, 754
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 3 cut(s) 298, 393, 858
AcuI CTGAAG 1 cut(s) 938
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 3 cut(s) 58, 356, 917
AgsI TTSAA 3 cut(s) 57, 356, 944
AjnI CCWGG 1 cut(s) 705
AluBI AGCT 5 cut(s) 217, 652, 676, 836, 940
AluI AGCT 5 cut(s) 217, 652, 676, 836, 940
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 5 cut(s) 62, 78, 833, 836, 845
ApoI RAATTY 3 cut(s) 298, 393, 858
ArsI GACNNNNNNTTYG 4 cut(s) 353, 385, 791, 823
AseI ATTAAT 1 cut(s) 978
AspA2I CCTAGG 1 cut(s) 911
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 2 cut(s) 124, 365
AsuHPI GGTGA 3 cut(s) 371, 743, 1000
AvaII GGWCC 1 cut(s) 683
AvrII CCTAGG 1 cut(s) 911
BalI TGGCCA 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 653
BbvI GCAGC 5 cut(s) 49, 65, 823, 832, 845
BccI CCATC 2 cut(s) 411, 471
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 1 cut(s) 223
BcnI CCSGG 2 cut(s) 124, 365
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 4 cut(s) 144, 591, 825, 912
BisI GCNGC 5 cut(s) 63, 79, 834, 837, 846
BlnI CCTAGG 1 cut(s) 911
BlsI GCNGC 5 cut(s) 64, 80, 835, 838, 847
Bme1390I CCNGG 3 cut(s) 124, 365, 707
Bme18I GGWCC 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 3 cut(s) 124, 365, 707
BmsI GCATC 1 cut(s) 753
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 124, 365
BsaJI CCNNGG 1 cut(s) 911
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 356, 917
Bse1I ACTGG 1 cut(s) 762
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 3 cut(s) 245, 499, 886
BseLI CCNNNNNNNGG 3 cut(s) 58, 356, 917
BseMII CTCAG 2 cut(s) 357, 644
BseNI ACTGG 1 cut(s) 762
BseRI GAGGAG 2 cut(s) 458, 670
BseXI GCAGC 5 cut(s) 49, 65, 823, 832, 845
Bsh1236I CGCG 3 cut(s) 426, 756, 866
BshFI GGCC 1 cut(s) 402
BsiSI CCGG 3 cut(s) 123, 249, 365
BslFI GGGAC 1 cut(s) 669
BslI CCNNNNNNNGG 3 cut(s) 58, 356, 917
BsmAI GTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 669
BsmI GAATGC 2 cut(s) 279, 835
BsnI GGCC 1 cut(s) 402
Bsp143I GATC 3 cut(s) 115, 223, 496
BspACI CCGC 4 cut(s) 44, 424, 611, 754
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 2 cut(s) 356, 645
BspFNI CGCG 3 cut(s) 426, 756, 866
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 685
BspPI GGATC 1 cut(s) 491
BsrI ACTGG 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 3 cut(s) 115, 223, 496
BssT1I CCWWGG 1 cut(s) 911
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 2 cut(s) 39, 203
BstC8I GCNNGC 2 cut(s) 83, 742
BstDEI CTNAG 4 cut(s) 343, 483, 653, 1038
BstEII GGTNACC 1 cut(s) 359
BstENI CCTNNNNNAGG 2 cut(s) 354, 915
BstF5I GGATG 3 cut(s) 245, 499, 886
BstFNI CGCG 3 cut(s) 426, 756, 866
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 3 cut(s) 118, 226, 499
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 115, 223, 496
BstMWI GCNNNNNNNGC 4 cut(s) 546, 842, 845, 872
BstNI CCWGG 1 cut(s) 707
BstPI GGTNACC 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 122, 363, 705
BstUI CGCG 3 cut(s) 426, 756, 866
BstV1I GCAGC 5 cut(s) 49, 65, 823, 832, 845
BstV2I GAAGAC 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 769
BsuRI GGCC 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 3 cut(s) 245, 499, 886
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 83, 742
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 226
CfoI GCGC 1 cut(s) 868
Cfr13I GGNCC 1 cut(s) 683
Csp6I GTAC 1 cut(s) 347
CviAII CATG 5 cut(s) 227, 388, 692, 802, 893
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 4 cut(s) 343, 483, 653, 1038
DpnI GATC 3 cut(s) 117, 225, 498
DpnII GATC 3 cut(s) 115, 223, 496
DraI TTTAAA 1 cut(s) 415
DraIII CACNNNGTG 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 911
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 683
Eco57I CTGAAG 1 cut(s) 938
Eco91I GGTNACC 1 cut(s) 359
EcoNI CCTNNNNNAGG 2 cut(s) 354, 915
EcoO109I RGGNCCY 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 705
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 911
ErhI CCWWGG 1 cut(s) 911
FaeI CATG 5 cut(s) 230, 391, 695, 805, 896
FaqI GGGAC 1 cut(s) 669
FatI CATG 5 cut(s) 226, 387, 691, 801, 892
FbaI TGATCA 1 cut(s) 223
Fnu4HI GCNGC 5 cut(s) 63, 79, 834, 837, 846
FokI GGATG 3 cut(s) 232, 506, 893
Fsp4HI GCNGC 5 cut(s) 63, 79, 834, 837, 846
FspBI CTAG 4 cut(s) 144, 591, 825, 912
GlaI GCGC 1 cut(s) 867
GluI GCNGC 5 cut(s) 63, 79, 834, 837, 846
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 1 cut(s) 402
HapII CCGG 3 cut(s) 123, 249, 365
HhaI GCGC 1 cut(s) 868
Hin1II CATG 5 cut(s) 230, 391, 695, 805, 896
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 5 cut(s) 18, 293, 428, 605, 658
HpaII CCGG 3 cut(s) 123, 249, 365
HphI GGTGA 3 cut(s) 371, 743, 1000
Hpy166II GTNNAC 2 cut(s) 188, 601
Hpy188I TCNGA 2 cut(s) 310, 663
Hpy188III TCNNGA 4 cut(s) 227, 560, 573, 591
Hpy8I GTNNAC 2 cut(s) 188, 601
HpyAV CCTTC 6 cut(s) 121, 350, 511, 697, 790, 979
HpyCH4III ACNGT 2 cut(s) 39, 203
HpyCH4IV ACGT 1 cut(s) 1018
HpyCH4V TGCA 6 cut(s) 81, 277, 287, 744, 833, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 546, 842, 845, 872
HpyF3I CTNAG 4 cut(s) 343, 483, 653, 1038
HpySE526I ACGT 1 cut(s) 1018
Hsp92II CATG 5 cut(s) 230, 391, 695, 805, 896
HspAI GCGC 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 3 cut(s) 115, 223, 496
Lsp1109I GCAGC 5 cut(s) 49, 65, 823, 832, 845
LweI GCATC 1 cut(s) 753
MaeI CTAG 4 cut(s) 144, 591, 825, 912
MaeII ACGT 1 cut(s) 1018
MaeIII GTNAC 4 cut(s) 68, 146, 359, 757
MalI GATC 3 cut(s) 117, 225, 498
MboI GATC 3 cut(s) 115, 223, 496
MboII GAAGA 5 cut(s) 471, 484, 655, 658, 1024
MfeI CAATTG 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 2 cut(s) 287, 667
MmeI TCCRAC 1 cut(s) 686
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 3 cut(s) 414, 530, 978
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 3 cut(s) 123, 249, 365
MspR9I CCNGG 3 cut(s) 124, 365, 707
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 2 cut(s) 279, 835
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 3 cut(s) 426, 756, 866
MwoI GCNNNNNNNGC 4 cut(s) 546, 842, 845, 872
NciI CCSGG 2 cut(s) 124, 365
NdeII GATC 3 cut(s) 115, 223, 496
NlaIII CATG 5 cut(s) 230, 391, 695, 805, 896
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 2 cut(s) 359, 757
PagI TCATGA 1 cut(s) 226
PctI GAATGC 2 cut(s) 279, 835
PfeI GAWTC 3 cut(s) 18, 428, 605
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 5 cut(s) 64, 80, 835, 838, 847
PleI GAGTC 2 cut(s) 287, 666
PpsI GAGTC 2 cut(s) 287, 666
PpuMI RGGWCCY 1 cut(s) 683
PshBI ATTAAT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 705
PspEI GGTNACC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 1 cut(s) 683
PspPPI RGGWCCY 1 cut(s) 683
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
SaqAI TTAA 3 cut(s) 414, 530, 978
SatI GCNGC 5 cut(s) 63, 79, 834, 837, 846
Sau3AI GATC 3 cut(s) 115, 223, 496
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 2 cut(s) 287, 667
ScrFI CCNGG 3 cut(s) 124, 365, 707
SfaNI GCATC 1 cut(s) 753
SinI GGWCC 1 cut(s) 683
SmlI CTYRAG 1 cut(s) 7
SmoI CTYRAG 1 cut(s) 7
SsiI CCGC 4 cut(s) 44, 424, 611, 754
SspMI CTAG 4 cut(s) 144, 591, 825, 912
StyD4I CCNGG 3 cut(s) 122, 363, 705
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 2 cut(s) 39, 203
TaiI ACGT 1 cut(s) 1021
TaqI TCGA 3 cut(s) 441, 794, 821
TfiI GAWTC 3 cut(s) 18, 428, 605
Tru1I TTAA 3 cut(s) 414, 530, 978
Tru9I TTAA 3 cut(s) 414, 530, 978
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 2 cut(s) 359, 757
TseI GCWGC 5 cut(s) 62, 78, 833, 836, 845
Tsp45I GTSAC 2 cut(s) 359, 757
TspDTI ATGAA 6 cut(s) 143, 234, 327, 633, 706, 818
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 683
VspI ATTAAT 1 cut(s) 978
XagI CCTNNNNNAGG 2 cut(s) 354, 915
XapI RAATTY 3 cut(s) 298, 393, 858
XbaI TCTAGA 1 cut(s) 590
XmaJI CCTAGG 1 cut(s) 911
XspI CTAG 4 cut(s) 144, 591, 825, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.