AT3G22620

Protease inhibitor seed storage lipid transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009903
Physical Location & Seq
Forward (+)
8008534 .. 8009590
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22620.1

Sequence Viewer

Length: 612 bp
ATGTCGAAGATTATTTCCCTTGTGGTGGCGATGATCGCAGTGCTAGCTTTGCCGATTCGTGGTCAACAACAACCTCTAAGCCAATGTACTCCATCAATGATGACCACCGTGAGTCCTTGTATGGGCTTCATAACCAACAGTAGCAGCAATGGAACTTCGCCGTCGTCTGATTGTTGTAACTCGCTGAGGTCTTTGACCACCGGAGGAATGGGATGTCTTTGTCTAATTGTCACTGGAACTGTTCCTTTCAATATTCCTATTAACCGCACAACCGCTGTCTCACTTCCCCGTGCTTGTAACATGCCTAGAGTCCCTCTTCAATGCCAAGCCAATATTGCTCCAGCTGCTGCTCCTGGACCTGCTGCTACATTTGGACCATCGATGTCTCCAGGACCAGAGACGGATCCAATTGTTCCAGAGCCAACTCCGGCAGCTCAGACACCACAGTCTGATACAACCCGACCATTTACACCATCCGTCGACGGTGGAGCTCCAACATCAGACGACGGAGGAAGCACCAGTCGACCTTCTGAGACTCCTTCATCCGCCTACGCACTCTCACCATCTCTTCTCTTCTTCAGCATCGCCCTCGTAGCTCTCAAATTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

203

Amino Acids

20.78

Weight (kDa)

6.1

Isoelectric Point (pI)

72.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 10 - 108 1.6e-22 Probable lipid transfer
Tryp_alpha_amyl PF00234 31 - 108 6.6e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 367
AccI GTMKAC 2 cut(s) 480, 523
AciI CCGC 3 cut(s) 265, 273, 546
AclWI GGATC 2 cut(s) 398, 411
AcsI RAATTY 1 cut(s) 602
AcuI CTGAAG 1 cut(s) 562
AfaI GTAC 1 cut(s) 88
AfiI CCNNNNNNNGG 3 cut(s) 25, 59, 122
AgsI TTSAA 2 cut(s) 250, 320
AjnI CCWGG 2 cut(s) 352, 388
AluBI AGCT 5 cut(s) 47, 344, 434, 491, 596
AluI AGCT 5 cut(s) 47, 344, 434, 491, 596
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 4 cut(s) 283, 390, 392, 527
AlwI GGATC 2 cut(s) 398, 411
AlwNI CAGNNNCTG 1 cut(s) 347
ApeKI GCWGC 5 cut(s) 144, 344, 347, 362, 431
ApoI RAATTY 1 cut(s) 602
AspS9I GGNCC 3 cut(s) 356, 374, 392
AsuHPI GGTGA 1 cut(s) 552
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 3 cut(s) 356, 374, 392
BamHI GGATCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 493
Bbv12I GWGCWC 1 cut(s) 493
BbvCI CCTCAGC 1 cut(s) 185
BbvI GCAGC 5 cut(s) 156, 331, 334, 349, 443
BccI CCATC 4 cut(s) 100, 385, 481, 571
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 571, 605
BciT130I CCWGG 2 cut(s) 354, 390
BcoDI GTCTC 4 cut(s) 283, 390, 392, 527
BfaI CTAG 2 cut(s) 44, 306
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 5 cut(s) 145, 345, 348, 363, 432
BlsI GCNGC 5 cut(s) 146, 346, 349, 364, 433
Bme1390I CCNGG 2 cut(s) 354, 390
Bme18I GGWCC 3 cut(s) 356, 374, 392
BmgT120I GGNCC 3 cut(s) 356, 374, 392
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 354, 390
BmsI GCATC 1 cut(s) 591
BmtI GCTAGC 1 cut(s) 47
BpmI CTGGAG 2 cut(s) 324, 372
Bpu10I CCTNAGC 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 380
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 59, 122
Bse1I ACTGG 2 cut(s) 238, 519
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 2 cut(s) 354, 390
BseCI ATCGAT 1 cut(s) 380
BseGI GGATG 3 cut(s) 218, 473, 542
BseLI CCNNNNNNNGG 3 cut(s) 25, 59, 122
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 3 cut(s) 176, 449, 522
BseNI ACTGG 2 cut(s) 238, 519
BseXI GCAGC 5 cut(s) 156, 331, 334, 349, 443
BshVI ATCGAT 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 2 cut(s) 201, 428
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 3 cut(s) 25, 59, 122
BsmAI GTCTC 4 cut(s) 283, 390, 392, 527
BsmBI CGTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 2 cut(s) 33, 403
BspACI CCGC 3 cut(s) 265, 273, 546
BspCNI CTCAG 3 cut(s) 177, 448, 523
BspDI ATCGAT 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 367
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 398, 411
BsrDI GCAATG 1 cut(s) 154
BsrI ACTGG 2 cut(s) 238, 519
BssMI GATC 2 cut(s) 33, 403
Bst2UI CCWGG 2 cut(s) 354, 390
Bst4CI ACNGT 5 cut(s) 109, 140, 241, 447, 485
Bst6I CTCTTC 3 cut(s) 321, 573, 578
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 4 cut(s) 77, 185, 435, 531
BstF5I GGATG 3 cut(s) 218, 473, 542
BstKTI GATC 2 cut(s) 36, 406
BstMAI GTCTC 4 cut(s) 283, 390, 392, 527
BstMBI GATC 2 cut(s) 33, 403
BstMWI GCNNNNNNNGC 6 cut(s) 35, 44, 49, 335, 344, 593
BstNI CCWGG 2 cut(s) 354, 390
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 352, 388
BstV1I GCAGC 5 cut(s) 156, 331, 334, 349, 443
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
Bsu15I ATCGAT 1 cut(s) 380
BsuTUI ATCGAT 1 cut(s) 380
BtgZI GCGATG 2 cut(s) 44, 568
BtsCI GGATG 3 cut(s) 218, 473, 542
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 231
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 1 cut(s) 45
CaiI CAGNNNCTG 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 356, 374, 392
ClaI ATCGAT 1 cut(s) 380
Csp6I GTAC 1 cut(s) 87
CviAII CATG 1 cut(s) 301
CviJI RGCY 9 cut(s) 47, 81, 126, 329, 344, 421, 434, 491, 596
CviKI_1 RGCY 9 cut(s) 47, 81, 126, 329, 344, 421, 434, 491, 596
CviQI GTAC 1 cut(s) 87
DdeI CTNAG 4 cut(s) 77, 185, 435, 531
DpnI GATC 2 cut(s) 35, 405
DpnII GATC 2 cut(s) 33, 403
DrdI GACNNNNNNGTC 1 cut(s) 445
DseDI GACNNNNNNGTC 1 cut(s) 445
Eam1104I CTCTTC 3 cut(s) 321, 573, 578
EarI CTCTTC 3 cut(s) 321, 573, 578
EciI GGCGGA 1 cut(s) 535
Ecl136II GAGCTC 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 493
Eco47I GGWCC 3 cut(s) 356, 374, 392
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 352, 388
EcoT38I GRGCYC 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 1 cut(s) 304
FaiI YATR 3 cut(s) 122, 131, 302
FaqI GGGAC 1 cut(s) 296
FatI CATG 1 cut(s) 300
FblI GTMKAC 2 cut(s) 480, 523
Fnu4HI GCNGC 5 cut(s) 145, 345, 348, 363, 432
FokI GGATG 3 cut(s) 225, 460, 529
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 5 cut(s) 145, 345, 348, 363, 432
FspBI CTAG 2 cut(s) 44, 306
GluI GCNGC 5 cut(s) 145, 345, 348, 363, 432
GsuI CTGGAG 2 cut(s) 324, 372
HapII CCGG 2 cut(s) 201, 428
Hin1II CATG 1 cut(s) 304
HincII GTYRAC 3 cut(s) 65, 481, 524
HindII GTYRAC 3 cut(s) 65, 481, 524
HinfI GANTC 4 cut(s) 55, 112, 309, 535
HpaII CCGG 2 cut(s) 201, 428
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 3 cut(s) 65, 481, 524
Hpy188I TCNGA 5 cut(s) 169, 438, 451, 502, 532
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 3 cut(s) 65, 481, 524
Hpy99I CGWCG 4 cut(s) 166, 482, 485, 509
HpyAV CCTTC 2 cut(s) 537, 549
HpyCH4III ACNGT 5 cut(s) 109, 140, 241, 447, 485
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 44, 49, 335, 344, 593
HpyF3I CTNAG 4 cut(s) 77, 185, 435, 531
Hsp92II CATG 1 cut(s) 304
Kzo9I GATC 2 cut(s) 33, 403
LmnI GCTCC 4 cut(s) 343, 355, 488, 496
Lsp1109I GCAGC 5 cut(s) 156, 331, 334, 349, 443
LweI GCATC 1 cut(s) 591
MaeI CTAG 2 cut(s) 44, 306
MaeIII GTNAC 3 cut(s) 176, 229, 296
MalI GATC 2 cut(s) 35, 405
MboI GATC 2 cut(s) 33, 403
MboII GAAGA 5 cut(s) 19, 308, 560, 565, 568
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 493
MluCI AATT 3 cut(s) 225, 408, 602
MlyI GAGTC 3 cut(s) 121, 318, 529
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 6 cut(s) 84, 180, 197, 324, 503, 599
MseI TTAA 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 275, 344
MspI CCGG 2 cut(s) 201, 428
MspR9I CCNGG 2 cut(s) 354, 390
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 2 cut(s) 354, 390
MwoI GCNNNNNNNGC 6 cut(s) 35, 44, 49, 335, 344, 593
NdeII GATC 2 cut(s) 33, 403
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 1 cut(s) 304
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 1 cut(s) 304
PfeI GAWTC 1 cut(s) 55
PfoI TCCNGGA 2 cut(s) 352, 388
PkrI GCNGC 5 cut(s) 146, 346, 349, 364, 433
PleI GAGTC 3 cut(s) 120, 317, 529
PpsI GAGTC 3 cut(s) 120, 317, 529
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 352, 388
PspGI CCWGG 2 cut(s) 352, 388
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 3 cut(s) 356, 374, 392
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 88
RsaNI GTAC 1 cut(s) 87
SacI GAGCTC 1 cut(s) 493
SalI GTCGAC 2 cut(s) 479, 522
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 5 cut(s) 145, 345, 348, 363, 432
Sau3AI GATC 2 cut(s) 33, 403
Sau96I GGNCC 3 cut(s) 356, 374, 392
SchI GAGTC 3 cut(s) 121, 318, 529
ScrFI CCNGG 2 cut(s) 354, 390
SduI GDGCHC 1 cut(s) 493
SetI ASST 9 cut(s) 49, 76, 191, 346, 361, 436, 493, 529, 598
SfaNI GCATC 1 cut(s) 591
SgrDI CGTCGACG 1 cut(s) 479
SinI GGWCC 3 cut(s) 356, 374, 392
Sse9I AATT 3 cut(s) 225, 408, 602
SsiI CCGC 3 cut(s) 265, 273, 546
SspI AATATT 2 cut(s) 253, 334
SspMI CTAG 2 cut(s) 44, 306
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 2 cut(s) 352, 388
TaaI ACNGT 5 cut(s) 109, 140, 241, 447, 485
TaqI TCGA 4 cut(s) 5, 380, 480, 523
TasI AATT 3 cut(s) 225, 408, 602
TatI WGTACW 1 cut(s) 86
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 45, 238
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 5 cut(s) 144, 344, 347, 362, 431
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 118, 531
TspGWI ACGGA 3 cut(s) 416, 466, 522
TspRI CASTG 2 cut(s) 45, 238
VpaK11BI GGWCC 3 cut(s) 356, 374, 392
XapI RAATTY 1 cut(s) 602
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmiI GTMKAC 2 cut(s) 480, 523
XspI CTAG 2 cut(s) 44, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.