Rorug04G0136100

RNA pol II accessory factor, Cdc73 family, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002623
Physical Location & Seq
Forward (+)
22085503 .. 22091521
6019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0136100.1

Sequence Viewer

Length: 3591 bp
ATGAGCACACAGTTGGATGCTTCTTCTTCTTCCTCGCCCTCCCCTTACCGGTGTACGTATCATGCATTCTTGAGTTTCAGAGGTGAGGACACACGTAAGGGATTTACAGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATTCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTACGATGTAGATCCTTCCCATGTCAGGAAGCAAAGCGGGAGTTTTGCTGAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATATTGCAGATCTTGGTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGATGATCGTGTGCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTTCCATAGATTTCATGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCCGAATGGTTTGGTTCGCTTGCAAAGAAATCTTCTTTCAGACCTCCAAAAGGGGAAAACAAAGAAAATATACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGAGCAGATGGTTTTTTCCAGGAAGTAAGATAATCTTAACAACCAGATACGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGGCTCAAGAGTTGAATGAGCATGAATCACTTGCGCTTCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCTGTTGTAAAACACTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGTAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCACATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGAGGAAAGAAAAAAGAGTTTTCAATTACAGTACTGGGCGACCTTAAATTCTACGCAGTGATTGGAATTCAAAATCTAGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTGGTACTGGAACTGTTAAGGGCATCATACTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGAAGTAACAGCAAACGACATCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTAGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCATCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAGATTTTCCAAAAAACTTGATATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCAATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTATGGAAGGGAACCAAGTTTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTTGAGAGATTGATTCTAAAAAATTGTAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCAACAAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCGTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTTATCACCTAACTTGTGGAAATCGTTCTTATCATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCGTTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGAATGCACAGAACTCAGAACGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACAGATTTTCTGGATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCATTGGAAGCTATGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGTCCCCATCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTAAACGGAAACGACATTCCAGATTGGTTTCGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGATATTGGTCGTTCTGCTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGTATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGAGGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGATCCGAGCTTGGATACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTATCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTGTTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000428 GO:0000988 GO:0000989 GO:0000993 GO:0001076 GO:0001098 GO:0001099 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0008213 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016071 GO:0016569 GO:0016570 GO:0016571 GO:0016591 GO:0016593 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030880 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034402 GO:0034622 GO:0034641 GO:0034968 GO:0036211 GO:0043170 GO:0043175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051276 GO:0051568 GO:0055029 GO:0060255 GO:0061458 GO:0061695 GO:0065003 GO:0065007 GO:0070013 GO:0070063 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0140110 GO:1901360 GO:1901564 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC73_C CDC73_N

Protein Analysis

1196

Amino Acids

135.33

Weight (kDa)

7.57

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 188 5.4e-55 TIR domain
TIR_2 PF13676 23 - 120 4.4e-18 TIR domain
NB-ARC PF00931 201 - 369 2.5e-18 NB-ARC domain
WHD_ROQ1 PF23282 440 - 511 9.5e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 820 - 913 4.7e-09 Leucine-rich repeat region
LRR_8 PF13855 820 - 874 3.8e-06 Leucine rich repeat
C-JID PF20160 989 - 1121 5.8e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AccB7I CCANNNNNTGG 1 cut(s) 3101
AccI GTMKAC 1 cut(s) 1741
AccII CGCG 1 cut(s) 1524
AciI CCGC 2 cut(s) 378, 1996
AclWI GGATC 4 cut(s) 347, 1207, 3285, 3298
AcuI CTGAAG 3 cut(s) 411, 756, 1342
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 2 cut(s) 1738, 3091
AfaI GTAC 7 cut(s) 55, 1392, 1606, 1875, 2409, 3033, 3270
AfeI AGCGCT 2 cut(s) 416, 459
AfiI CCNNNNNNNGG 5 cut(s) 48, 366, 829, 2926, 3101
AflII CTTAAG 1 cut(s) 2473
AflIII ACRYGT 6 cut(s) 92, 147, 322, 621, 3210, 3271
AgeI ACCGGT 1 cut(s) 48
AhdI GACNNNNNGTC 1 cut(s) 3153
AjnI CCWGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
AjuI GAANNNNNNNTTGG 2 cut(s) 3527, 3559
Alw21I GWGCWC 4 cut(s) 8, 135, 2655, 3475
AlwI GGATC 4 cut(s) 347, 1207, 3285, 3298
Aor51HI AGCGCT 2 cut(s) 416, 459
AoxI GGCC 2 cut(s) 123, 2077
ApeKI GCWGC 4 cut(s) 191, 707, 744, 2004
AseI ATTAAT 1 cut(s) 1461
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 3 cut(s) 1726, 1899, 2390
AspLEI GCGC 4 cut(s) 417, 460, 1087, 1241
AspS9I GGNCC 4 cut(s) 123, 1540, 2917, 3165
AsuHPI GGTGA 5 cut(s) 95, 1524, 1747, 2364, 2522
AvaII GGWCC 3 cut(s) 1540, 2917, 3165
AxyI CCTNAGG 1 cut(s) 1536
BamHI GGATCC 1 cut(s) 3290
BanII GRGCYC 3 cut(s) 135, 2655, 3542
BarI GAAGNNNNNNTAC 2 cut(s) 1845, 1877
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 3 cut(s) 1473, 1653, 3213
Bbv12I GWGCWC 4 cut(s) 8, 135, 2655, 3475
BbvCI CCTCAGC 2 cut(s) 1780, 2874
BbvI GCAGC 4 cut(s) 203, 694, 756, 2016
BccI CCATC 8 cut(s) 424, 629, 963, 1125, 1712, 2930, 3365, 3517
BceAI ACGGC 1 cut(s) 1751
BciT130I CCWGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
BciVI GTATCC 3 cut(s) 2531, 2734, 3296
BclI TGATCA 4 cut(s) 1318, 2826, 2886, 3583
BfaI CTAG 6 cut(s) 246, 1436, 1451, 1979, 2343, 2952
BfoI RGCGCY 2 cut(s) 418, 461
BfrI CTTAAG 1 cut(s) 2473
BfuI GTATCC 3 cut(s) 2531, 2734, 3296
BglII AGATCT 2 cut(s) 475, 2588
BisI GCNGC 4 cut(s) 192, 708, 745, 2005
BlpI GCTNAGC 2 cut(s) 2479, 3221
BlsI GCNGC 4 cut(s) 193, 709, 746, 2006
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
Bme18I GGWCC 3 cut(s) 1540, 2917, 3165
BmeRI GACNNNNNGTC 1 cut(s) 3153
BmgT120I GGNCC 4 cut(s) 123, 1540, 2917, 3165
BmiI GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
BmrFI CCNGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
BmrI ACTGGG 1 cut(s) 1403
BmsI GCATC 8 cut(s) 7, 874, 1495, 1564, 1632, 1638, 1808, 1830
BmuI ACTGGG 1 cut(s) 1403
BpiI GAAGAC 3 cut(s) 1473, 1653, 3213
BpmI CTGGAG 3 cut(s) 1779, 2651, 3417
Bpu10I CCTNAGC 2 cut(s) 1780, 2874
Bpu1102I GCTNAGC 2 cut(s) 2479, 3221
BpuEI CTTGAG 5 cut(s) 91, 1041, 2138, 2624, 3403
Bsa29I ATCGAT 1 cut(s) 2168
BsaAI YACGTR 4 cut(s) 57, 95, 325, 3272
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 2 cut(s) 1738, 3091
BsaI GGTCTC 2 cut(s) 3140, 3161
BsaJI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 2649, 2679, 3252, 3282
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 366, 829, 2926, 3101
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 3 cut(s) 573, 2018, 3148
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
BseCI ATCGAT 1 cut(s) 2168
BseDI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BseGI GGATG 4 cut(s) 22, 1117, 2922, 3413
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 48, 366, 829, 2926, 3101
BseMI GCAATG 3 cut(s) 573, 2018, 3148
BseMII CTCAG 8 cut(s) 948, 1105, 1527, 1794, 2454, 2650, 2865, 3488
BseNI ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
BseRI GAGGAG 3 cut(s) 192, 440, 2518
BseXI GCAGC 4 cut(s) 203, 694, 756, 2016
Bsh1236I CGCG 1 cut(s) 1524
BshFI GGCC 2 cut(s) 125, 2079
BshTI ACCGGT 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 2168
BsiHKAI GWGCWC 4 cut(s) 8, 135, 2655, 3475
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 2853, 2903
BslI CCNNNNNNNGG 5 cut(s) 48, 366, 829, 2926, 3101
BsmBI CGTCTC 2 cut(s) 150, 3255
BsmFI GGGAC 2 cut(s) 2853, 2903
BsmI GAATGC 3 cut(s) 65, 1829, 2630
BsnI GGCC 2 cut(s) 125, 2079
Bso31I GGTCTC 2 cut(s) 3140, 3161
Bsp1286I GDGCHC 5 cut(s) 8, 135, 2655, 3475, 3542
Bsp1407I TGTACA 1 cut(s) 1873
Bsp1720I GCTNAGC 2 cut(s) 2479, 3221
Bsp68I TCGCGA 1 cut(s) 1524
BspACI CCGC 2 cut(s) 378, 1996
BspANI GGCC 2 cut(s) 125, 2079
BspCNI CTCAG 8 cut(s) 947, 1104, 1528, 1793, 2453, 2649, 2866, 3487
BspDI ATCGAT 1 cut(s) 2168
BspFNI CGCG 1 cut(s) 1524
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
BspPI GGATC 4 cut(s) 347, 1207, 3285, 3298
BspQI GCTCTTC 2 cut(s) 405, 448
BspTI CTTAAG 1 cut(s) 2473
BspTNI GGTCTC 2 cut(s) 3140, 3161
BsrDI GCAATG 3 cut(s) 573, 2018, 3148
BsrFI RCCGGY 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 1873
BsrI ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BssNI GRCGYC 2 cut(s) 1738, 3091
BssT1I CCWWGG 2 cut(s) 126, 3150
Bst2UI CCWGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
Bst4CI ACNGT 6 cut(s) 12, 504, 1166, 1390, 1615, 2844
Bst6I CTCTTC 4 cut(s) 405, 448, 1757, 2265
BstACI GRCGYC 2 cut(s) 1738, 3091
BstAFI CTTAAG 1 cut(s) 2473
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstAUI TGTACA 1 cut(s) 1873
BstBAI YACGTR 4 cut(s) 57, 95, 325, 3272
BstC8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2009, 2679
BstENI CCTNNNNNAGG 1 cut(s) 827
BstF5I GGATG 4 cut(s) 22, 1117, 2922, 3413
BstFNI CGCG 1 cut(s) 1524
BstH2I RGCGCY 2 cut(s) 418, 461
BstHHI GCGC 4 cut(s) 417, 460, 1087, 1241
BstMWI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1927, 2650, 2952
BstNI CCWGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
BstNSI RCATGY 4 cut(s) 625, 1353, 3075, 3214
BstSCI CCNGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
BstSNI TACGTA 1 cut(s) 57
BstUI CGCG 1 cut(s) 1524
BstV1I GCAGC 4 cut(s) 203, 694, 756, 2016
BstV2I GAAGAC 3 cut(s) 1473, 1653, 3213
BstX2I RGATCY 5 cut(s) 352, 475, 1199, 2588, 3290
BstYI RGATCY 5 cut(s) 352, 475, 1199, 2588, 3290
Bsu15I ATCGAT 1 cut(s) 2168
Bsu36I CCTNAGG 1 cut(s) 1536
BsuI GTATCC 3 cut(s) 2531, 2734, 3296
BsuRI GGCC 2 cut(s) 125, 2079
BsuTUI ATCGAT 1 cut(s) 2168
BtsCI GGATG 4 cut(s) 22, 1117, 2922, 3413
BtsI GCAGTG 1 cut(s) 1422
BtsIMutI CAGTG 5 cut(s) 342, 1162, 1422, 2811, 3414
BtuMI TCGCGA 1 cut(s) 1524
Cac8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2009, 2679
CciI TCATGA 1 cut(s) 1321
CfoI GCGC 4 cut(s) 417, 460, 1087, 1241
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 4 cut(s) 123, 1540, 2917, 3165
ClaI ATCGAT 1 cut(s) 2168
CseI GACGC 4 cut(s) 326, 1727, 2288, 3099
Csp6I GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
CspAI ACCGGT 1 cut(s) 48
CviQI GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
DraI TTTAAA 3 cut(s) 1944, 2854, 3133
DriI GACNNNNNGTC 1 cut(s) 3153
Eam1104I CTCTTC 4 cut(s) 405, 448, 1757, 2265
Eam1105I GACNNNNNGTC 1 cut(s) 3153
EarI CTCTTC 4 cut(s) 405, 448, 1757, 2265
Ecl136II GAGCTC 2 cut(s) 133, 2653
Eco105I TACGTA 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 126, 3150
Eco24I GRGCYC 3 cut(s) 135, 2655, 3542
Eco31I GGTCTC 2 cut(s) 3140, 3161
Eco47I GGWCC 3 cut(s) 1540, 2917, 3165
Eco47III AGCGCT 2 cut(s) 416, 459
Eco53kI GAGCTC 2 cut(s) 133, 2653
Eco57I CTGAAG 3 cut(s) 411, 756, 1342
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 1536
EcoICRI GAGCTC 2 cut(s) 133, 2653
EcoNI CCTNNNNNAGG 1 cut(s) 827
EcoO109I RGGNCCY 3 cut(s) 123, 1540, 2917
EcoRI GAATTC 3 cut(s) 141, 1425, 3054
EcoRII CCWGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
EcoT14I CCWWGG 2 cut(s) 126, 3150
EcoT22I ATGCAT 2 cut(s) 67, 3073
EcoT38I GRGCYC 3 cut(s) 135, 2655, 3542
ErhI CCWWGG 2 cut(s) 126, 3150
Esp3I CGTCTC 2 cut(s) 150, 3255
FalI AAGNNNNNCTT 4 cut(s) 980, 1012, 3527, 3559
FaqI GGGAC 2 cut(s) 2853, 2903
FauI CCCGC 1 cut(s) 371
FbaI TGATCA 4 cut(s) 1318, 2826, 2886, 3583
FblI GTMKAC 1 cut(s) 1741
Fnu4HI GCNGC 4 cut(s) 192, 708, 745, 2005
FokI GGATG 4 cut(s) 29, 1104, 2909, 3400
FriOI GRGCYC 3 cut(s) 135, 2655, 3542
Fsp4HI GCNGC 4 cut(s) 192, 708, 745, 2005
FspBI CTAG 6 cut(s) 246, 1436, 1451, 1979, 2343, 2952
GlaI GCGC 4 cut(s) 416, 459, 1086, 1240
GluI GCNGC 4 cut(s) 192, 708, 745, 2005
GsuI CTGGAG 3 cut(s) 1779, 2651, 3417
HaeII RGCGCY 2 cut(s) 418, 461
HaeIII GGCC 2 cut(s) 125, 2079
HapII CCGG 1 cut(s) 49
HgaI GACGC 4 cut(s) 326, 1727, 2288, 3099
HhaI GCGC 4 cut(s) 417, 460, 1087, 1241
Hin1I GRCGYC 2 cut(s) 1738, 3091
Hin6I GCGC 4 cut(s) 415, 458, 1085, 1239
HinP1I GCGC 4 cut(s) 415, 458, 1085, 1239
HincII GTYRAC 3 cut(s) 1039, 1742, 1813
HindII GTYRAC 3 cut(s) 1039, 1742, 1813
HindIII AAGCTT 8 cut(s) 393, 1128, 2228, 2291, 2470, 2476, 2600, 2782
HpaI GTTAAC 1 cut(s) 1039
HpaII CCGG 1 cut(s) 49
HphI GGTGA 5 cut(s) 95, 1524, 1747, 2364, 2522
Hpy166II GTNNAC 8 cut(s) 54, 928, 1039, 1742, 1813, 2790, 3165, 3435
Hpy8I GTNNAC 8 cut(s) 54, 928, 1039, 1742, 1813, 2790, 3165, 3435
Hpy99I CGWCG 2 cut(s) 1743, 3093
HpyAV CCTTC 7 cut(s) 366, 857, 1349, 1588, 2017, 2908, 3553
HpyCH4III ACNGT 6 cut(s) 12, 504, 1166, 1390, 1615, 2844
HpyCH4IV ACGT 7 cut(s) 56, 94, 149, 301, 324, 2458, 3271
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1927, 2650, 2952
HpySE526I ACGT 7 cut(s) 56, 94, 149, 301, 324, 2458, 3271
Hsp92I GRCGYC 2 cut(s) 1738, 3091
HspAI GCGC 4 cut(s) 415, 458, 1085, 1239
Ksp22I TGATCA 4 cut(s) 1318, 2826, 2886, 3583
KspAI GTTAAC 1 cut(s) 1039
LguI GCTCTTC 2 cut(s) 405, 448
LmnI GCTCC 4 cut(s) 130, 179, 583, 2658
Lsp1109I GCAGC 4 cut(s) 203, 694, 756, 2016
LweI GCATC 8 cut(s) 7, 874, 1495, 1564, 1632, 1638, 1808, 1830
MaeI CTAG 6 cut(s) 246, 1436, 1451, 1979, 2343, 2952
MaeII ACGT 7 cut(s) 56, 94, 149, 301, 324, 2458, 3271
MaeIII GTNAC 4 cut(s) 198, 1685, 2328, 3422
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 5 cut(s) 352, 475, 1199, 2588, 3290
MhlI GDGCHC 5 cut(s) 8, 135, 2655, 3475, 3542
MlyI GAGTC 5 cut(s) 227, 1298, 1667, 1786, 2100
MmeI TCCRAC 2 cut(s) 2258, 3364
Mph1103I ATGCAT 2 cut(s) 67, 3073
MroXI GAANNNNTTC 3 cut(s) 1726, 1899, 2390
MslI CAYNNNNRTG 6 cut(s) 265, 1572, 2073, 2822, 2927, 3567
MspCI CTTAAG 1 cut(s) 2473
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 3 cut(s) 65, 1829, 2630
MvaI CCWGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
MvnI CGCG 1 cut(s) 1524
MwoI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1927, 2650, 2952
NlaIV GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 2328
NruI TCGCGA 1 cut(s) 1524
NsiI ATGCAT 2 cut(s) 67, 3073
NspI RCATGY 4 cut(s) 625, 1353, 3075, 3214
PaeI GCATGC 1 cut(s) 1353
PagI TCATGA 1 cut(s) 1321
PasI CCCWGGG 1 cut(s) 1544
PciI ACATGT 2 cut(s) 621, 3210
PciSI GCTCTTC 2 cut(s) 405, 448
PcsI WCGNNNNNNNCGW 1 cut(s) 2464
PctI GAATGC 3 cut(s) 65, 1829, 2630
PdmI GAANNNNTTC 3 cut(s) 1726, 1899, 2390
PflFI GACNNNGTC 2 cut(s) 1737, 1746
PflMI CCANNNNNTGG 1 cut(s) 3101
PfoI TCCNGGA 1 cut(s) 979
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 4 cut(s) 193, 709, 746, 2006
PleI GAGTC 5 cut(s) 226, 1298, 1666, 1786, 2100
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 5 cut(s) 226, 1298, 1666, 1786, 2100
Ppu21I YACGTR 4 cut(s) 57, 95, 325, 3272
PpuMI RGGWCCY 2 cut(s) 1540, 2917
PscI ACATGT 2 cut(s) 621, 3210
PshBI ATTAAT 1 cut(s) 1461
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 2 cut(s) 135, 2655
Psp5II RGGWCCY 2 cut(s) 1540, 2917
Psp6I CCWGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
PspCI CACGTG 1 cut(s) 325
PspGI CCWGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
PspN4I GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
PspPI GGNCC 4 cut(s) 123, 1540, 2917, 3165
PspPPI RGGWCCY 2 cut(s) 1540, 2917
PsuI RGATCY 5 cut(s) 352, 475, 1199, 2588, 3290
PsyI GACNNNGTC 2 cut(s) 1737, 1746
RruI TCGCGA 1 cut(s) 1524
RsaI GTAC 7 cut(s) 55, 1392, 1606, 1875, 2409, 3033, 3270
RsaNI GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
RseI CAYNNNNRTG 6 cut(s) 265, 1572, 2073, 2822, 2927, 3567
SacI GAGCTC 2 cut(s) 135, 2655
SalI GTCGAC 1 cut(s) 1740
SapI GCTCTTC 2 cut(s) 405, 448
SatI GCNGC 4 cut(s) 192, 708, 745, 2005
Sau96I GGNCC 4 cut(s) 123, 1540, 2917, 3165
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 5 cut(s) 227, 1298, 1667, 1786, 2100
ScrFI CCNGG 6 cut(s) 981, 1545, 1759, 2508, 2927, 3500
SduI GDGCHC 5 cut(s) 8, 135, 2655, 3475, 3542
SfaNI GCATC 8 cut(s) 7, 874, 1495, 1564, 1632, 1638, 1808, 1830
SinI GGWCC 3 cut(s) 1540, 2917, 3165
SmiMI CAYNNNNRTG 6 cut(s) 265, 1572, 2073, 2822, 2927, 3567
SmlI CTYRAG 6 cut(s) 70, 1056, 2117, 2473, 2603, 3418
SmoI CTYRAG 6 cut(s) 70, 1056, 2117, 2473, 2603, 3418
SnaBI TACGTA 1 cut(s) 57
SphI GCATGC 1 cut(s) 1353
SsiI CCGC 2 cut(s) 378, 1996
SspI AATATT 1 cut(s) 1585
SspMI CTAG 6 cut(s) 246, 1436, 1451, 1979, 2343, 2952
SstI GAGCTC 2 cut(s) 135, 2655
StyD4I CCNGG 6 cut(s) 979, 1543, 1757, 2506, 2925, 3498
StyI CCWWGG 2 cut(s) 126, 3150
TaaI ACNGT 6 cut(s) 12, 504, 1166, 1390, 1615, 2844
TaiI ACGT 7 cut(s) 59, 97, 152, 304, 327, 2461, 3274
TaqI TCGA 6 cut(s) 171, 221, 1498, 1741, 2168, 3058
TatI WGTACW 2 cut(s) 1390, 1873
TscAI CASTG 5 cut(s) 342, 1169, 1422, 2818, 3414
TseFI GTSAC 1 cut(s) 2328
TseI GCWGC 4 cut(s) 191, 707, 744, 2004
Tsp45I GTSAC 1 cut(s) 2328
TspGWI ACGGA 2 cut(s) 570, 2982
TspRI CASTG 5 cut(s) 342, 1169, 1422, 2818, 3414
Tth111I GACNNNGTC 2 cut(s) 1737, 1746
Van91I CCANNNNNTGG 1 cut(s) 3101
Vha464I CTTAAG 1 cut(s) 2473
VpaK11BI GGWCC 3 cut(s) 1540, 2917, 3165
VspI ATTAAT 1 cut(s) 1461
XagI CCTNNNNNAGG 1 cut(s) 827
XbaI TCTAGA 1 cut(s) 245
XceI RCATGY 4 cut(s) 625, 1353, 3075, 3214
XcmI CCANNNNNNNNNTGG 2 cut(s) 734, 3233
XmiI GTMKAC 1 cut(s) 1741
XmnI GAANNNNTTC 3 cut(s) 1726, 1899, 2390
XspI CTAG 6 cut(s) 246, 1436, 1451, 1979, 2343, 2952
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 2 cut(s) 67, 3073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.