AT3G22820

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011794
Physical Location & Seq
Reverse (-)
8073258 .. 8074178
921 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22820.1

Sequence Viewer

Length: 324 bp
ATGGGCGTCGTCCTCCCAACTCTCATCGTTTATGCTTTCCTTCTCTTCTTCTCGTCGTCCTCCGCCGCCTCTCTTCAACGTCCCAGTGGAGGGCTTGGGCAGGGGAAGAAGGAGATAGCTAGGTCGGGTTTACCGGGTCAGATTGTAGATCAGAAGCGGTTAGGGGGACCTGGCTCGGTGCCTCCGATGTGTAGATTGAAGTGTGGGAAATGTGAGCCTTGTAAAGCAGTTCACGTTCCGATTCAACCAGGTCTGATTATGCCACTGGAGTATTACCCTGAAGCTTGGCGCTGCAAGTGTGGCAACAAACTCTTCATGCCCTAA

Protein Analysis

107

Amino Acids

11.5

Weight (kDa)

9.47

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 57 - 107 5.3e-19 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 178
AciI CCGC 3 cut(s) 63, 66, 157
AcuI CTGAAG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 6
AfiI CCNNNNNNNGG 2 cut(s) 89, 90
AgsI TTSAA 3 cut(s) 77, 199, 245
AjnI CCWGG 2 cut(s) 169, 247
AluBI AGCT 2 cut(s) 119, 284
AluI AGCT 2 cut(s) 119, 284
ApeKI GCWGC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 167
BanI GGYRCC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 171, 249
BcnI CCSGG 1 cut(s) 135
BfaI CTAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 292
BisI GCNGC 2 cut(s) 66, 292
BlsI GCNGC 2 cut(s) 67, 293
Bme1390I CCNGG 3 cut(s) 135, 171, 249
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 168, 180
BmrFI CCNGG 3 cut(s) 135, 171, 249
BmrI ACTGGG 1 cut(s) 78
BmuI ACTGGG 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 6
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 90
Bse1I ACTGG 2 cut(s) 84, 270
BseBI CCWGG 2 cut(s) 171, 249
BseLI CCNNNNNNNGG 2 cut(s) 89, 90
BseNI ACTGG 2 cut(s) 84, 270
BseXI GCAGC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 178
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 2 cut(s) 66, 180
BslI CCNNNNNNNGG 2 cut(s) 89, 90
BsmFI GGGAC 2 cut(s) 66, 180
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 3 cut(s) 63, 66, 157
BspLI GGNNCC 2 cut(s) 168, 180
BspT107I GGYRCC 1 cut(s) 178
BsrI ACTGG 2 cut(s) 84, 270
BssMI GATC 1 cut(s) 148
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 171, 249
Bst6I CTCTTC 3 cut(s) 50, 78, 317
BstACI GRCGYC 1 cut(s) 6
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstNI CCWGG 2 cut(s) 171, 249
BstSCI CCNGG 3 cut(s) 133, 169, 247
BstV1I GCAGC 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 91, 263
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 167
CsiI ACCWGGT 1 cut(s) 247
CviAII CATG 1 cut(s) 316
CviJI RGCY 5 cut(s) 94, 119, 174, 217, 284
CviKI_1 RGCY 5 cut(s) 94, 119, 174, 217, 284
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 3 cut(s) 50, 78, 317
EarI CTCTTC 3 cut(s) 50, 78, 317
EciI GGCGGA 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 169, 247
FaeI CATG 1 cut(s) 319
FaiI YATR 3 cut(s) 33, 260, 317
FaqI GGGAC 2 cut(s) 66, 180
FatI CATG 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 66, 292
Fsp4HI GCNGC 2 cut(s) 66, 292
FspBI CTAG 1 cut(s) 120
GlaI GCGC 1 cut(s) 290
GluI GCNGC 2 cut(s) 66, 292
GsuI CTGGAG 1 cut(s) 287
HaeII RGCGCY 1 cut(s) 292
HapII CCGG 1 cut(s) 134
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 1 cut(s) 241
HpaII CCGG 1 cut(s) 134
Hpy166II GTNNAC 2 cut(s) 131, 232
Hpy188I TCNGA 5 cut(s) 141, 153, 186, 240, 255
Hpy8I GTNNAC 2 cut(s) 131, 232
Hpy99I CGWCG 2 cut(s) 11, 58
HpyAV CCTTC 2 cut(s) 50, 103
HpyCH4IV ACGT 2 cut(s) 79, 234
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpySE526I ACGT 2 cut(s) 79, 234
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 1 cut(s) 148
LpnPI CCDG 9 cut(s) 86, 97, 147, 156, 183, 234, 251, 261, 291
Lsp1109I GCAGC 1 cut(s) 278
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 1 cut(s) 120
MaeII ACGT 2 cut(s) 79, 234
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 5 cut(s) 37, 40, 65, 118, 304
MnlI CCTC 5 cut(s) 23, 70, 79, 83, 192
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 3 cut(s) 135, 171, 249
MvaI CCWGG 2 cut(s) 171, 249
MwoI GCNNNNNNNGC 1 cut(s) 300
NciI CCSGG 1 cut(s) 135
NdeII GATC 1 cut(s) 148
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 2 cut(s) 168, 180
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 2 cut(s) 67, 293
PpuMI RGGWCCY 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 2 cut(s) 169, 247
PspGI CCWGG 2 cut(s) 169, 247
PspN4I GGNNCC 2 cut(s) 168, 180
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
SatI GCNGC 2 cut(s) 66, 292
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 3 cut(s) 135, 171, 249
SetI ASST 7 cut(s) 82, 121, 125, 172, 237, 253, 286
SexAI ACCWGGT 1 cut(s) 247
SinI GGWCC 1 cut(s) 167
SsiI CCGC 3 cut(s) 63, 66, 157
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 3 cut(s) 133, 169, 247
TaiI ACGT 2 cut(s) 82, 237
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 241
TscAI CASTG 2 cut(s) 91, 270
TseI GCWGC 1 cut(s) 291
TspDTI ATGAA 1 cut(s) 304
TspRI CASTG 2 cut(s) 91, 270
VpaK11BI GGWCC 1 cut(s) 167
XspI CTAG 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.