FvH4_4g01110

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011794
Physical Location & Seq
Reverse (-)
1041759 .. 1043775
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01110.t1

Sequence Viewer

Length: 351 bp
ATGGGCGTACTGCGCCACCGCCGCCCACATCACCATTCCACCCTCACATGTACAGCAGCCTTGGCTTTCCTCTTCATCATAACCTCAGCCTCCGCCATACGTACTACTCCAACCCAACTCGGTGACACCGAAGTAGTCAGTGGTTCGTCTATACCGGAAGATGAGCTTACGGCGGAGAAGAGACAGCTGGGAGGGCCGGGCTCTGCTCCGCCACGATGTAGATCCAAGTGTGGAAGATGCTCGCCCTGCAAGGCGGTCCACGTACCCATTCAGCCAGGTCTGAGCATACCTCTCGAGTATTATCCAGAAGCTTGGCGCTGCAAGTGCGGCAATAAGCTCTTCATGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

117

Amino Acids

12.6

Weight (kDa)

9.16

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 66 - 116 3.4e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 19, 22, 93, 173, 209, 254, 327
AclWI GGATC 1 cut(s) 216
AfaI GTAC 4 cut(s) 9, 52, 103, 264
AflIII ACRYGT 1 cut(s) 47
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 4 cut(s) 166, 187, 311, 337
AluI AGCT 4 cut(s) 166, 187, 311, 337
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 2 cut(s) 56, 318
AspLEI GCGC 2 cut(s) 15, 318
AspS9I GGNCC 2 cut(s) 194, 256
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 23, 134
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 85
BbvI GCAGC 2 cut(s) 68, 305
BceAI ACGGC 1 cut(s) 186
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 1 cut(s) 276
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 175
BfoI RGCGCY 1 cut(s) 319
BisI GCNGC 4 cut(s) 22, 57, 319, 328
BlsI GCNGC 4 cut(s) 23, 58, 320, 329
Bme1390I CCNGG 2 cut(s) 198, 276
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 194, 256
BmrFI CCNGG 2 cut(s) 198, 276
BmsI GCATC 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 274, 306
Bpu10I CCTNAGC 1 cut(s) 85
BpuMI CCSGG 1 cut(s) 198
BsaAI YACGTR 2 cut(s) 101, 262
BsaBI GATNNNNATC 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 154
Bse8I GATNNNNATC 1 cut(s) 220
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 60
BseJI GATNNNNATC 1 cut(s) 220
BseMII CTCAG 2 cut(s) 99, 272
BseXI GCAGC 2 cut(s) 68, 305
BseYI CCCAGC 1 cut(s) 187
BshFI GGCC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 155, 197
BsmAI GTCTC 1 cut(s) 175
BsnI GGCC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 50
Bsp143I GATC 1 cut(s) 221
BspACI CCGC 7 cut(s) 19, 22, 93, 173, 209, 254, 327
BspANI GGCC 1 cut(s) 196
BspCNI CTCAG 2 cut(s) 98, 273
BspPI GGATC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 344
BsrGI TGTACA 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 60
BssMI GATC 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 276
Bst6I CTCTTC 3 cut(s) 77, 173, 344
BstAUI TGTACA 1 cut(s) 50
BstBAI YACGTR 2 cut(s) 101, 262
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 85, 281
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 2 cut(s) 15, 318
BstKTI GATC 1 cut(s) 224
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 1 cut(s) 221
BstMWI GCNNNNNNNGC 8 cut(s) 12, 21, 62, 193, 246, 324, 327, 343
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 196, 274
BstSNI TACGTA 1 cut(s) 101
BstV1I GCAGC 2 cut(s) 68, 305
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 312
BstYI RGATCY 1 cut(s) 221
BsuRI GGCC 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 242
CfoI GCGC 2 cut(s) 15, 318
Cfr13I GGNCC 2 cut(s) 194, 256
Csp6I GTAC 4 cut(s) 8, 51, 102, 263
CviAII CATG 2 cut(s) 48, 343
CviQI GTAC 4 cut(s) 8, 51, 102, 263
DdeI CTNAG 2 cut(s) 85, 281
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
Eam1104I CTCTTC 3 cut(s) 77, 173, 344
EarI CTCTTC 3 cut(s) 77, 173, 344
EciI GGCGGA 3 cut(s) 82, 188, 198
Eco105I TACGTA 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 256
Eco88I CYCGRG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 2 cut(s) 51, 346
FaiI YATR 6 cut(s) 49, 80, 98, 152, 287, 344
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FatI CATG 2 cut(s) 47, 342
Fnu4HI GCNGC 4 cut(s) 22, 57, 319, 328
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 4 cut(s) 22, 57, 319, 328
GlaI GCGC 2 cut(s) 14, 317
GluI GCNGC 4 cut(s) 22, 57, 319, 328
GsaI CCCAGC 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 1 cut(s) 196
HapII CCGG 2 cut(s) 155, 197
HhaI GCGC 2 cut(s) 15, 318
Hin1II CATG 2 cut(s) 51, 346
Hin6I GCGC 2 cut(s) 13, 316
HinP1I GCGC 2 cut(s) 13, 316
HindIII AAGCTT 1 cut(s) 309
HpaII CCGG 2 cut(s) 155, 197
HphI GGTGA 2 cut(s) 23, 134
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 2 cut(s) 293, 305
Hpy8I GTNNAC 1 cut(s) 259
HpyCH4IV ACGT 2 cut(s) 100, 261
HpyCH4V TGCA 2 cut(s) 249, 321
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 21, 62, 193, 246, 324, 327, 343
HpyF3I CTNAG 2 cut(s) 85, 281
HpySE526I ACGT 2 cut(s) 100, 261
Hsp92II CATG 2 cut(s) 51, 346
HspAI GCGC 2 cut(s) 13, 316
Kzo9I GATC 1 cut(s) 221
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 7 cut(s) 168, 173, 210, 259, 261, 288, 318
Lsp1109I GCAGC 2 cut(s) 68, 305
LweI GCATC 1 cut(s) 227
MaeII ACGT 2 cut(s) 100, 261
MaeIII GTNAC 1 cut(s) 122
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MboII GAAGA 5 cut(s) 64, 170, 190, 246, 331
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 6 cut(s) 53, 80, 94, 100, 185, 300
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 2 cut(s) 155, 197
MspR9I CCNGG 2 cut(s) 198, 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 8 cut(s) 12, 21, 62, 193, 246, 324, 327, 343
NciI CCSGG 1 cut(s) 198
NdeII GATC 1 cut(s) 221
NlaIII CATG 2 cut(s) 51, 346
NmuCI GTSAC 1 cut(s) 122
NspI RCATGY 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 293
PciI ACATGT 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PkrI GCNGC 4 cut(s) 23, 58, 320, 329
Ppu21I YACGTR 2 cut(s) 101, 262
PscI ACATGT 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 274
PspPI GGNCC 2 cut(s) 194, 256
PsuI RGATCY 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 4 cut(s) 9, 52, 103, 264
RsaNI GTAC 4 cut(s) 8, 51, 102, 263
SapI GCTCTTC 1 cut(s) 344
SatI GCNGC 4 cut(s) 22, 57, 319, 328
Sau3AI GATC 1 cut(s) 221
Sau96I GGNCC 2 cut(s) 194, 256
ScrFI CCNGG 2 cut(s) 198, 276
SduI GDGCHC 1 cut(s) 203
SetI ASST 9 cut(s) 86, 103, 168, 189, 264, 280, 292, 313, 339
SfaNI GCATC 1 cut(s) 227
Sfr274I CTCGAG 1 cut(s) 293
SinI GGWCC 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SnaBI TACGTA 1 cut(s) 101
SsiI CCGC 7 cut(s) 19, 22, 93, 173, 209, 254, 327
StyD4I CCNGG 2 cut(s) 196, 274
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 2 cut(s) 103, 264
TaqI TCGA 1 cut(s) 294
TatI WGTACW 1 cut(s) 50
TauI GCSGC 2 cut(s) 24, 330
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 2 cut(s) 56, 318
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 64, 331
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 256
XceI RCATGY 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.