MD16G1199100.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011794
Physical Location & Seq
Reverse (-)
17981500 .. 17982466
967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1199100.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGGCGTACTGCGCCGCTCCCGCCACCGCCACCACAACCACCATTATATAAGGCCATCAACTGTCATCACAGCCTTCACTTTTCTCCTGTTCACCTCAGCCTCAACGGCAGTAACCAGTCAAACCCAACTCGGAGGTGGTGGGCATCATCATCATCATTATCAAGGTGAAAGCGGGCCAGCATTGGAGCGAGTCGTGACCCACCAAAAGATTCGTCTGGGTTCATCGCCACCAAGTTGCAGATCCAAGTGCGGGAGCTGCTCGCCGTGTAAGGCAGTTCACGTCCCCATCCAACCAGGTGTCTCGATACCTCTCGAGTACTACCCCGAAGCTTGGCGCTGCAAGTGTGGCAGCCATCTCTTCATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

124

Amino Acids

13.62

Weight (kDa)

9.75

Isoelectric Point (pI)

72.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 72 - 123 2.7e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 18
AciI CCGC 5 cut(s) 16, 22, 28, 174, 252
AclWI GGATC 1 cut(s) 237
AfaI GTAC 2 cut(s) 9, 320
AfiI CCNNNNNNNGG 2 cut(s) 252, 333
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 2 cut(s) 258, 332
AluI AGCT 2 cut(s) 258, 332
Alw26I GTCTC 1 cut(s) 307
AlwI GGATC 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 2 cut(s) 53, 176
ApeKI GCWGC 3 cut(s) 258, 339, 351
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 2 cut(s) 15, 339
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 85, 179
AvaI CYCGRG 1 cut(s) 314
BbvCI CCTCAGC 1 cut(s) 97
BbvI GCAGC 3 cut(s) 245, 326, 363
BccI CCATC 3 cut(s) 64, 296, 363
BceAI ACGGC 2 cut(s) 123, 250
BciT130I CCWGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 307
BfaI CTAG 1 cut(s) 370
BfoI RGCGCY 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 4 cut(s) 16, 259, 340, 352
BlsI GCNGC 4 cut(s) 17, 260, 341, 353
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 154
Bpu10I CCTNAGC 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 333
Bse1I ACTGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 252, 333
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 245, 326, 363
BshFI GGCC 2 cut(s) 55, 178
BsiHKCI CYCGRG 1 cut(s) 314
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 252, 333
BsmAI GTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 2 cut(s) 55, 178
BsoBI CYCGRG 1 cut(s) 314
Bsp143I GATC 1 cut(s) 242
BspACI CCGC 5 cut(s) 16, 22, 28, 174, 252
BspANI GGCC 2 cut(s) 55, 178
BspCNI CTCAG 1 cut(s) 110
BspPI GGATC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 18
BsrI ACTGG 1 cut(s) 117
BssMI GATC 1 cut(s) 242
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 176, 180, 263
BstDEI CTNAG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 2 cut(s) 15, 339
BstKTI GATC 1 cut(s) 245
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 1 cut(s) 242
BstMWI GCNNNNNNNGC 5 cut(s) 12, 21, 107, 258, 348
BstNI CCWGG 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 295
BstV1I GCAGC 3 cut(s) 245, 326, 363
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuRI GGCC 2 cut(s) 55, 178
BtgZI GCGATG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 288
Cac8I GCNNGC 3 cut(s) 176, 180, 263
CfoI GCGC 2 cut(s) 15, 339
Cfr13I GGNCC 1 cut(s) 176
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 2 cut(s) 8, 319
CviAII CATG 1 cut(s) 364
CviJI RGCY 7 cut(s) 55, 74, 101, 178, 258, 332, 354
CviKI_1 RGCY 7 cut(s) 55, 74, 101, 178, 258, 332, 354
CviQI GTAC 2 cut(s) 8, 319
DdeI CTNAG 1 cut(s) 97
DpnI GATC 1 cut(s) 244
DpnII GATC 1 cut(s) 242
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 367
FaiI YATR 3 cut(s) 48, 50, 365
FaqI GGGAC 1 cut(s) 269
FatI CATG 1 cut(s) 363
FauI CCCGC 3 cut(s) 29, 167, 245
Fnu4HI GCNGC 4 cut(s) 16, 259, 340, 352
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 4 cut(s) 16, 259, 340, 352
FspBI CTAG 1 cut(s) 370
GlaI GCGC 2 cut(s) 14, 338
GluI GCNGC 4 cut(s) 16, 259, 340, 352
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 2 cut(s) 55, 178
HhaI GCGC 2 cut(s) 15, 339
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 2 cut(s) 13, 337
HinP1I GCGC 2 cut(s) 13, 337
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 2 cut(s) 192, 211
HphI GGTGA 2 cut(s) 85, 179
Hpy166II GTNNAC 2 cut(s) 93, 280
Hpy188I TCNGA 1 cut(s) 134
Hpy188III TCNNGA 3 cut(s) 196, 304, 314
Hpy8I GTNNAC 2 cut(s) 93, 280
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 240, 342
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 21, 107, 258, 348
HpyF3I CTNAG 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 2 cut(s) 13, 337
Kzo9I GATC 1 cut(s) 242
LmnI GCTCC 3 cut(s) 23, 187, 255
LpnPI CCDG 6 cut(s) 101, 130, 192, 203, 282, 309
Lsp1109I GCAGC 3 cut(s) 245, 326, 363
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 1 cut(s) 370
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 112, 196
MalI GATC 1 cut(s) 244
MbiI CCGCTC 1 cut(s) 18
MboI GATC 1 cut(s) 242
MboII GAAGA 1 cut(s) 352
MflI RGATCY 1 cut(s) 242
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 4 cut(s) 106, 112, 128, 321
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 12, 21, 107, 258, 348
NdeII GATC 1 cut(s) 242
NlaIII CATG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 314
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 4 cut(s) 17, 260, 341, 353
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspPI GGNCC 1 cut(s) 176
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 2 cut(s) 9, 320
RsaNI GTAC 2 cut(s) 8, 319
SatI GCNGC 4 cut(s) 16, 259, 340, 352
Sau3AI GATC 1 cut(s) 242
Sau96I GGNCC 1 cut(s) 176
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 297
SetI ASST 8 cut(s) 98, 139, 169, 260, 285, 301, 313, 334
SexAI ACCWGGT 1 cut(s) 295
SfaNI GCATC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 314
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SsiI CCGC 5 cut(s) 16, 22, 28, 174, 252
SspMI CTAG 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 285
TaqI TCGA 2 cut(s) 305, 315
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 1 cut(s) 211
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 3 cut(s) 258, 339, 351
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 213, 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 1 cut(s) 370
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.