pycom16g16770

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011794
Physical Location & Seq
Reverse (-)
12768674 .. 12769172
499 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16770.1

Sequence Viewer

Length: 369 bp
ATGGGCGTGCTGCGCCGCTCCCGCCACCGCCACCACAACCACCACTATATAAGGCCATCAACCGTCATCACAGCCTTCACTTTTCTCCTATTCACCTCAACCTCAACGGCAGTAACCACTCAAACCCAACTTGGAGGTGGTGGGCATCATCATCATTATCAAGGTGAAAGCGGGCCAGCATTGGAGCGAGTCGTGACCCACCAGAAGATTCGTCTGGGTTCATCGCCACCAAGTTGCAGATCCAAGTGCGGGAGCTGCTCGCCGTGTAAGGCAGTTCACGTCGCCATCCAACCAGGTGTCTCGATACCTCTCGAGTACTACCCCGAAGCTTGGCGCTGCAAGTGTGGCAACCATCTCTTCATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

123

Amino Acids

13.52

Weight (kDa)

9.75

Isoelectric Point (pI)

68.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 71 - 122 1e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 18
AciI CCGC 5 cut(s) 16, 22, 28, 171, 249
AclWI GGATC 1 cut(s) 234
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 249, 330
AjiI CACGTC 1 cut(s) 280
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 2 cut(s) 255, 329
AluI AGCT 2 cut(s) 255, 329
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 234
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 53, 173
ApeKI GCWGC 3 cut(s) 10, 255, 336
AspLEI GCGC 2 cut(s) 15, 336
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 85, 176
AvaI CYCGRG 1 cut(s) 311
BbvI GCAGC 2 cut(s) 242, 323
BccI CCATC 3 cut(s) 64, 293, 360
BceAI ACGGC 2 cut(s) 123, 247
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 1 cut(s) 367
BfoI RGCGCY 1 cut(s) 337
BisI GCNGC 4 cut(s) 11, 16, 256, 337
BlsI GCNGC 4 cut(s) 12, 17, 257, 338
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 294
BmeT110I CYCGRG 1 cut(s) 311
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 154
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 330
BseBI CCWGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 249, 330
BseXI GCAGC 2 cut(s) 242, 323
BshFI GGCC 2 cut(s) 55, 175
BsiHKCI CYCGRG 1 cut(s) 311
BslI CCNNNNNNNGG 2 cut(s) 249, 330
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 2 cut(s) 55, 175
BsoBI CYCGRG 1 cut(s) 311
Bsp143I GATC 1 cut(s) 239
BspACI CCGC 5 cut(s) 16, 22, 28, 171, 249
BspANI GGCC 2 cut(s) 55, 175
BspPI GGATC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 18
BssMI GATC 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 362
BstC8I GCNNGC 4 cut(s) 8, 173, 177, 260
BstF5I GGATG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 15, 336
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 1 cut(s) 239
BstMWI GCNNNNNNNGC 4 cut(s) 12, 21, 255, 345
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 242, 323
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 2 cut(s) 55, 175
BtgZI GCGATG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 1 cut(s) 285
Cac8I GCNNGC 4 cut(s) 8, 173, 177, 260
CfoI GCGC 2 cut(s) 15, 336
Cfr13I GGNCC 1 cut(s) 173
CsiI ACCWGGT 1 cut(s) 292
Csp6I GTAC 1 cut(s) 316
CviAII CATG 1 cut(s) 361
CviJI RGCY 5 cut(s) 55, 74, 175, 255, 329
CviKI_1 RGCY 5 cut(s) 55, 74, 175, 255, 329
CviQI GTAC 1 cut(s) 316
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 292
FaeI CATG 1 cut(s) 364
FaiI YATR 3 cut(s) 48, 50, 362
FatI CATG 1 cut(s) 360
FauI CCCGC 3 cut(s) 29, 164, 242
Fnu4HI GCNGC 4 cut(s) 11, 16, 256, 337
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 4 cut(s) 11, 16, 256, 337
FspBI CTAG 1 cut(s) 367
GlaI GCGC 2 cut(s) 14, 335
GluI GCNGC 4 cut(s) 11, 16, 256, 337
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 2 cut(s) 55, 175
HhaI GCGC 2 cut(s) 15, 336
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 2 cut(s) 13, 334
HinP1I GCGC 2 cut(s) 13, 334
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 2 cut(s) 189, 208
HphI GGTGA 2 cut(s) 85, 176
Hpy166II GTNNAC 1 cut(s) 277
Hpy188III TCNNGA 3 cut(s) 193, 301, 311
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 2 cut(s) 237, 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 21, 255, 345
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 2 cut(s) 13, 334
Kzo9I GATC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 23, 184, 252
LpnPI CCDG 5 cut(s) 189, 200, 215, 279, 306
Lsp1109I GCAGC 2 cut(s) 242, 323
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 292
MaeI CTAG 1 cut(s) 367
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 2 cut(s) 112, 193
MalI GATC 1 cut(s) 241
MbiI CCGCTC 1 cut(s) 18
MboI GATC 1 cut(s) 239
MboII GAAGA 2 cut(s) 217, 349
MflI RGATCY 1 cut(s) 239
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 4 cut(s) 106, 112, 128, 318
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 4 cut(s) 12, 21, 255, 345
NdeII GATC 1 cut(s) 239
NlaIII CATG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 311
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 4 cut(s) 12, 17, 257, 338
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspPI GGNCC 1 cut(s) 173
PsuI RGATCY 1 cut(s) 239
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SatI GCNGC 4 cut(s) 11, 16, 256, 337
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 173
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 1 cut(s) 294
SetI ASST 9 cut(s) 98, 104, 139, 166, 257, 282, 298, 310, 331
SexAI ACCWGGT 1 cut(s) 292
SfaNI GCATC 1 cut(s) 154
Sfr274I CTCGAG 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SsiI CCGC 5 cut(s) 16, 22, 28, 171, 249
SspMI CTAG 1 cut(s) 367
StyD4I CCNGG 1 cut(s) 292
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 302, 312
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 1 cut(s) 208
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 3 cut(s) 10, 255, 336
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 210, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 311
XspI CTAG 1 cut(s) 367
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.