Rorug03G0312400

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011794
Physical Location & Seq
Reverse (-)
33437146 .. 33438214
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0312400.1

Sequence Viewer

Length: 663 bp
ATGGAGAGAATTAGGAAGCAGAGCAGAGGTGGCGATGAATCGGCCTTGGTAAAGGCGGCCGCATGGGCATGGTATGAGCATGGGACTGGATTAGAAGGCAAGTCACTGCAGGAATTTGATATCAGAAGAACTTCTCATGATCCTAAACCATCCAGGTTCAAGCTAGAAGCTAAGAAATTAGCCGAACAAGGCAGTAGAGTTTTTGATGAAGGGTCAAGTCCGGGAACTAGTGGGAGTGTACAGACTGTTGATGATCATTCACTTCTTGATAGATATGAAATTGAGAGTATTTCTAGGCGGTTGGATCAACTTATAGAGTCTAGTGGTCACAAAGGCACAGTTTATAATAAGGGGTTCCTAGTTGGAGATCGTCTTGCTAAACAGTACGACCACTCTAATCTTGGATGCGGTAGTAGATGCTCACCGCCGCCGAAGTTACAAGCTCCCACGTCTGCAGTTGGAGATCTTCGTCAAAAAGGAAGTGTGGCATTATCAGATGGTGACTACACAAATCGCAGGAGGAAGAAAACGAGCAACATCTTTAGAGGGTTTTGGCAAAGGCATGTAGTGATATGCGGTTCAAGTACTCATAAAGATGTAATTCTAGGGACAAGATCTTTTGTGGATAATCGGGGGCAGCAGCAGAACCCGGAAAAGCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

220

Amino Acids

24.56

Weight (kDa)

9.65

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AciI CCGC 8 cut(s) 56, 60, 298, 408, 425, 428, 576, 658
AclWI GGATC 2 cut(s) 134, 312
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 113
AfaI GTAC 3 cut(s) 240, 386, 586
AgsI TTSAA 2 cut(s) 160, 582
AhlI ACTAGT 1 cut(s) 227
AjiI CACGTC 1 cut(s) 450
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 3 cut(s) 163, 170, 443
AluI AGCT 3 cut(s) 163, 170, 443
AlwI GGATC 2 cut(s) 134, 312
AoxI GGCC 2 cut(s) 42, 57
ApeKI GCWGC 2 cut(s) 637, 640
ApoI RAATTY 1 cut(s) 113
Asp700I GAANNNNTTC 1 cut(s) 130
AsuC2I CCSGG 2 cut(s) 222, 650
AsuHPI GGTGA 2 cut(s) 414, 512
BbvI GCAGC 2 cut(s) 649, 652
BccI CCATC 2 cut(s) 157, 491
BciT130I CCWGG 1 cut(s) 154
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 2 cut(s) 222, 650
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 6 cut(s) 164, 228, 294, 321, 359, 605
BfmI CTRYAG 2 cut(s) 107, 453
BglI GCCNNNNNGGC 1 cut(s) 65
BglII AGATCT 2 cut(s) 463, 614
BisI GCNGC 5 cut(s) 57, 60, 428, 638, 641
BlsI GCNGC 5 cut(s) 58, 61, 429, 639, 642
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 3 cut(s) 154, 222, 650
BmgBI CACGTC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 356
BmrFI CCNGG 3 cut(s) 154, 222, 650
BmsI GCATC 2 cut(s) 395, 407
BpuMI CCSGG 2 cut(s) 222, 650
BsaJI CCNNGG 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 91
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 149, 410
BseNI ACTGG 1 cut(s) 91
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 649, 652
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 44, 59
BsiEI CGRYCG 1 cut(s) 60
BsiSI CCGG 2 cut(s) 221, 650
BslFI GGGAC 2 cut(s) 97, 622
BsmFI GGGAC 2 cut(s) 97, 622
BsnI GGCC 2 cut(s) 44, 59
Bsp1407I TGTACA 1 cut(s) 238
Bsp143I GATC 6 cut(s) 139, 253, 304, 367, 463, 614
BspACI CCGC 8 cut(s) 56, 60, 298, 408, 425, 428, 576, 658
BspANI GGCC 2 cut(s) 44, 59
BspHI TCATGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 356
BspMAI CTGCAG 2 cut(s) 111, 457
BspPI GGATC 2 cut(s) 134, 312
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 6 cut(s) 139, 253, 304, 367, 463, 614
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 3 cut(s) 247, 340, 384
BstAUI TGTACA 1 cut(s) 238
BstDEI CTNAG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 149, 410
BstKTI GATC 6 cut(s) 142, 256, 307, 370, 466, 617
BstMBI GATC 6 cut(s) 139, 253, 304, 367, 463, 614
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 30, 65
BstNI CCWGG 1 cut(s) 154
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 3 cut(s) 152, 220, 648
BstSFI CTRYAG 2 cut(s) 107, 453
BstV1I GCAGC 2 cut(s) 649, 652
BstX2I RGATCY 2 cut(s) 463, 614
BstYI RGATCY 2 cut(s) 463, 614
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 44, 59
BtgZI GCGATG 1 cut(s) 48
BtrI CACGTC 1 cut(s) 450
BtsCI GGATG 2 cut(s) 149, 410
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 104
CciI TCATGA 1 cut(s) 136
CciNI GCGGCCGC 1 cut(s) 57
Csp6I GTAC 3 cut(s) 239, 385, 585
CviAII CATG 5 cut(s) 63, 69, 80, 137, 563
CviJI RGCY 6 cut(s) 44, 59, 163, 170, 182, 443
CviKI_1 RGCY 6 cut(s) 44, 59, 163, 170, 182, 443
CviQI GTAC 3 cut(s) 239, 385, 585
DdeI CTNAG 1 cut(s) 171
DpnI GATC 6 cut(s) 141, 255, 306, 369, 465, 616
DpnII GATC 6 cut(s) 139, 253, 304, 367, 463, 614
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 45
Eco32I GATATC 1 cut(s) 121
Eco52I CGGCCG 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 152
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 5 cut(s) 66, 72, 83, 140, 566
FaqI GGGAC 2 cut(s) 97, 622
FatI CATG 5 cut(s) 62, 68, 79, 136, 562
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 5 cut(s) 57, 60, 428, 638, 641
FokI GGATG 2 cut(s) 136, 417
Fsp4HI GCNGC 5 cut(s) 57, 60, 428, 638, 641
FspBI CTAG 6 cut(s) 164, 228, 294, 321, 359, 605
GluI GCNGC 5 cut(s) 57, 60, 428, 638, 641
HaeIII GGCC 2 cut(s) 44, 59
HapII CCGG 2 cut(s) 221, 650
Hin1II CATG 5 cut(s) 66, 72, 83, 140, 566
HinfI GANTC 2 cut(s) 38, 317
HpaII CCGG 2 cut(s) 221, 650
HphI GGTGA 2 cut(s) 414, 512
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 2 cut(s) 125, 496
Hpy188III TCNNGA 2 cut(s) 137, 266
Hpy8I GTNNAC 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 89, 203
HpyCH4III ACNGT 3 cut(s) 247, 340, 384
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 2 cut(s) 109, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 65
HpyF3I CTNAG 1 cut(s) 171
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 5 cut(s) 66, 72, 83, 140, 566
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 6 cut(s) 139, 253, 304, 367, 463, 614
LmnI GCTCC 1 cut(s) 448
LpnPI CCDG 6 cut(s) 72, 95, 139, 166, 234, 502
Lsp1109I GCAGC 2 cut(s) 649, 652
LweI GCATC 2 cut(s) 395, 407
MaeI CTAG 6 cut(s) 164, 228, 294, 321, 359, 605
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 4 cut(s) 102, 326, 435, 500
MalI GATC 6 cut(s) 141, 255, 306, 369, 465, 616
MboI GATC 6 cut(s) 139, 253, 304, 367, 463, 614
MboII GAAGA 3 cut(s) 138, 458, 535
MflI RGATCY 2 cut(s) 463, 614
MluCI AATT 5 cut(s) 9, 113, 176, 279, 600
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 3 cut(s) 282, 343, 439
MnlI CCTC 3 cut(s) 20, 513, 539
MroXI GAANNNNTTC 1 cut(s) 130
MslI CAYNNNNRTG 2 cut(s) 67, 594
MspI CCGG 2 cut(s) 221, 650
MspR9I CCNGG 3 cut(s) 154, 222, 650
MvaI CCWGG 1 cut(s) 154
MwoI GCNNNNNNNGC 2 cut(s) 30, 65
NciI CCSGG 2 cut(s) 222, 650
NdeII GATC 6 cut(s) 139, 253, 304, 367, 463, 614
NlaIII CATG 5 cut(s) 66, 72, 83, 140, 566
NlaIV GGNNCC 1 cut(s) 356
NmuCI GTSAC 3 cut(s) 102, 326, 500
NotI GCGGCCGC 1 cut(s) 57
NspI RCATGY 1 cut(s) 566
PagI TCATGA 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 38
PfoI TCCNGGA 1 cut(s) 220
PkrI GCNGC 5 cut(s) 58, 61, 429, 639, 642
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
PsiI TTATAA 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 152
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 1 cut(s) 356
PstI CTGCAG 2 cut(s) 111, 457
PsuI RGATCY 2 cut(s) 463, 614
RsaI GTAC 3 cut(s) 240, 386, 586
RsaNI GTAC 3 cut(s) 239, 385, 585
RseI CAYNNNNRTG 2 cut(s) 67, 594
SatI GCNGC 5 cut(s) 57, 60, 428, 638, 641
Sau3AI GATC 6 cut(s) 139, 253, 304, 367, 463, 614
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 3 cut(s) 154, 222, 650
SetI ASST 6 cut(s) 31, 158, 165, 172, 445, 452
SfaNI GCATC 2 cut(s) 395, 407
SfcI CTRYAG 2 cut(s) 107, 453
SmiMI CAYNNNNRTG 2 cut(s) 67, 594
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 5 cut(s) 9, 113, 176, 279, 600
SsiI CCGC 8 cut(s) 56, 60, 298, 408, 425, 428, 576, 658
SspMI CTAG 6 cut(s) 164, 228, 294, 321, 359, 605
StyD4I CCNGG 3 cut(s) 152, 220, 648
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 3 cut(s) 247, 340, 384
TaiI ACGT 1 cut(s) 452
TasI AATT 5 cut(s) 9, 113, 176, 279, 600
TatI WGTACW 2 cut(s) 238, 584
TauI GCSGC 3 cut(s) 59, 62, 430
TfiI GAWTC 1 cut(s) 38
TscAI CASTG 1 cut(s) 111
TseFI GTSAC 3 cut(s) 102, 326, 500
TseI GCWGC 2 cut(s) 637, 640
Tsp45I GTSAC 3 cut(s) 102, 326, 500
TspDTI ATGAA 3 cut(s) 51, 222, 291
TspRI CASTG 1 cut(s) 111
XapI RAATTY 1 cut(s) 113
XceI RCATGY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 130
XspI CTAG 6 cut(s) 164, 228, 294, 321, 359, 605
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.