AT4G14723

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011794
Physical Location & Seq
Forward (+)
8439899 .. 8440901
1003 bp
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UTR
Exon/CDS
Intron
AT4G14723.1

Sequence Viewer

Length: 330 bp
ATGGGCACCTTCCGTCGCCGCCGTCGCTTTCTTTTAGCTGCACTCGTCACATTTGCTCTCCTCCACCTCTTCTCCGCCTCCTCCATAGTCTCCGCCGATGGTAGATGGATTGGTCAGAGAACCGGGTCGGATTTACCGGGTGGGTTCATAAGGAGTAATAAGCGGTTTGGTGGTCCGGGTTCATCACCACCGACGTGTAGATCGAAGTGTGGGAAATGTCAGCCGTGTAAACCGGTTCACGTACCAATTCAACCCGGTTTAAGTATGCCATTGGAGTATTACCCTGAAGCTTGGCGGTGCAAGTGTGGCAACAAGCTCTTCATGCCCTAA

Protein Analysis

109

Amino Acids

12.06

Weight (kDa)

10.45

Isoelectric Point (pI)

77.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 59 - 109 3.4e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 5 cut(s) 19, 75, 93, 163, 295
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 1 cut(s) 243
AflIII ACRYGT 1 cut(s) 194
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 1 cut(s) 251
AjiI CACGTC 1 cut(s) 195
AleI CACNNNNGTG 1 cut(s) 193
AluBI AGCT 3 cut(s) 38, 290, 316
AluI AGCT 3 cut(s) 38, 290, 316
Alw26I GTCTC 1 cut(s) 94
ApeKI GCWGC 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 4 cut(s) 124, 138, 177, 255
AsuHPI GGTGA 1 cut(s) 177
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 5
BbvI GCAGC 1 cut(s) 25
BccI CCATC 2 cut(s) 92, 99
BceAI ACGGC 2 cut(s) 6, 208
BcnI CCSGG 4 cut(s) 124, 138, 177, 255
BcoDI GTCTC 1 cut(s) 94
BisI GCNGC 2 cut(s) 19, 39
BlsI GCNGC 2 cut(s) 20, 40
Bme1390I CCNGG 4 cut(s) 124, 138, 177, 255
Bme18I GGWCC 1 cut(s) 173
BmgBI CACGTC 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 4 cut(s) 124, 138, 177, 255
BpuMI CCSGG 4 cut(s) 124, 138, 177, 255
BsaAI YACGTR 1 cut(s) 241
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bse118I RCCGGY 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 50, 70
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 1 cut(s) 24
BshNI GGYRCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 232
BsiSI CCGG 5 cut(s) 123, 137, 176, 233, 255
BsmAI GTCTC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 200
BspACI CCGC 5 cut(s) 19, 75, 93, 163, 295
BspLI GGNNCC 1 cut(s) 7
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 5
BsrFI RCCGGY 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 232
BssMI GATC 1 cut(s) 200
Bst6I CTCTTC 2 cut(s) 74, 323
BstBAI YACGTR 1 cut(s) 241
BstKTI GATC 1 cut(s) 203
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 3 cut(s) 24, 306, 322
BstSCI CCNGG 4 cut(s) 122, 136, 175, 253
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 25
BtrI CACGTC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 242
CspAI ACCGGT 1 cut(s) 232
CviAII CATG 1 cut(s) 322
CviJI RGCY 4 cut(s) 38, 223, 290, 316
CviKI_1 RGCY 4 cut(s) 38, 223, 290, 316
CviQI GTAC 1 cut(s) 242
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
Eam1104I CTCTTC 2 cut(s) 74, 323
EarI CTCTTC 2 cut(s) 74, 323
EciI GGCGGA 2 cut(s) 64, 82
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 306
FaeI CATG 1 cut(s) 325
FaiI YATR 4 cut(s) 86, 149, 266, 323
FatI CATG 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 19, 39
Fsp4HI GCNGC 2 cut(s) 19, 39
GluI GCNGC 2 cut(s) 19, 39
HapII CCGG 5 cut(s) 123, 137, 176, 233, 255
Hin1II CATG 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 288
HpaII CCGG 5 cut(s) 123, 137, 176, 233, 255
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 230, 238
Hpy188I TCNGA 2 cut(s) 117, 130
Hpy8I GTNNAC 2 cut(s) 230, 238
Hpy99I CGWCG 3 cut(s) 18, 27, 196
HpyAV CCTTC 1 cut(s) 19
HpyCH4IV ACGT 2 cut(s) 194, 240
HpyCH4V TGCA 2 cut(s) 41, 300
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 306, 322
HpySE526I ACGT 2 cut(s) 194, 240
Hsp92II CATG 1 cut(s) 325
Kzo9I GATC 1 cut(s) 200
LguI GCTCTTC 1 cut(s) 323
LpnPI CCDG 6 cut(s) 136, 150, 189, 246, 268, 297
Lsp1109I GCAGC 1 cut(s) 25
MaeII ACGT 2 cut(s) 194, 240
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 2 cut(s) 61, 310
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 4 cut(s) 71, 77, 88, 91
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 193
MspI CCGG 5 cut(s) 123, 137, 176, 233, 255
MspR9I CCNGG 4 cut(s) 124, 138, 177, 255
MwoI GCNNNNNNNGC 3 cut(s) 24, 306, 322
NciI CCSGG 4 cut(s) 124, 138, 177, 255
NdeII GATC 1 cut(s) 200
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 1 cut(s) 46
OliI CACNNNNGTG 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 2 cut(s) 20, 40
Ppu21I YACGTR 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 173
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 193
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 2 cut(s) 19, 39
Sau3AI GATC 1 cut(s) 200
Sau96I GGNCC 1 cut(s) 173
ScrFI CCNGG 4 cut(s) 124, 138, 177, 255
SduI GDGCHC 1 cut(s) 8
SetI ASST 7 cut(s) 11, 40, 69, 197, 243, 292, 318
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 193
Sse9I AATT 1 cut(s) 246
SsiI CCGC 5 cut(s) 19, 75, 93, 163, 295
StyD4I CCNGG 4 cut(s) 122, 136, 175, 253
TaiI ACGT 2 cut(s) 197, 243
TaqI TCGA 1 cut(s) 203
TasI AATT 1 cut(s) 246
TauI GCSGC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 3 cut(s) 136, 171, 310
VpaK11BI GGWCC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.