MD13G1199400.v1.1

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011794
Physical Location & Seq
Reverse (-)
17705623 .. 17706652
1030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1199400.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGGGGTACTGCGCCACCGCCACAACCACCATTATATAAGGCTATCAACTGTCATCACGGCCTTCACTTTTCTCTTGTTCACCTCAGCCTCAACGGCCGTAACCAGTCAAACCCAACTCGGTGGTGGGCATCATCATCAAGGTGAAGGTGGTGGGCCAGCACTGGAGCGAGTCGTGACCCACCAGAAGAGTCGACTGGGTTCATCGCCACCGAGTTGCAGATCCAAGTGCGGGAGGTGCTCGCCGTGTAAGGCAGTGCATGTCCCCATCCAACCAGGTGTCTCTATACCTCTCGAGTACTACCCCGAAGCTTGGCGCTGCAAGTGTGGCAACCATCTCTTCATGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

117

Amino Acids

12.7

Weight (kDa)

9.63

Isoelectric Point (pI)

56.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 65 - 116 7.8e-21 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 193
AciI CCGC 2 cut(s) 19, 231
AclWI GGATC 1 cut(s) 216
AcoI YGGCCR 1 cut(s) 96
AfaI GTAC 2 cut(s) 9, 299
AfiI CCNNNNNNNGG 2 cut(s) 231, 312
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 3 cut(s) 60, 96, 155
ApeKI GCWGC 1 cut(s) 318
AspLEI GCGC 2 cut(s) 15, 318
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 73, 155
AvaI CYCGRG 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 242
BbvCI CCTCAGC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 305
BccI CCATC 2 cut(s) 275, 342
BceAI ACGGC 4 cut(s) 75, 83, 111, 229
BciT130I CCWGG 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 319
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 320
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 155
BmrFI CCNGG 1 cut(s) 276
BmrI ACTGGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 85
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 312
Bse1I ACTGG 3 cut(s) 105, 168, 201
BseBI CCWGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 2 cut(s) 231, 312
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 3 cut(s) 105, 168, 201
BseX3I CGGCCG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 305
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 62, 98, 157
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 293
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 2 cut(s) 231, 312
BsmAI GTCTC 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 3 cut(s) 62, 98, 157
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 1 cut(s) 221
BspACI CCGC 2 cut(s) 19, 231
BspANI GGCC 3 cut(s) 62, 98, 157
BspCNI CTCAG 1 cut(s) 98
BspPI GGATC 1 cut(s) 216
BsrI ACTGG 3 cut(s) 105, 168, 201
BssMI GATC 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 52
Bst6I CTCTTC 2 cut(s) 182, 344
BstC8I GCNNGC 2 cut(s) 159, 242
BstDEI CTNAG 1 cut(s) 85
BstF5I GGATG 1 cut(s) 267
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 2 cut(s) 15, 318
BstKTI GATC 1 cut(s) 224
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 1 cut(s) 221
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 95, 237, 327
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 221
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 221
BstZI CGGCCG 1 cut(s) 96
BsuRI GGCC 3 cut(s) 62, 98, 157
BtgZI GCGATG 1 cut(s) 189
BtsCI GGATG 1 cut(s) 267
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 161, 261
Cac8I GCNNGC 2 cut(s) 159, 242
CfoI GCGC 2 cut(s) 15, 318
Cfr13I GGNCC 1 cut(s) 155
CsiI ACCWGGT 1 cut(s) 274
Csp6I GTAC 2 cut(s) 8, 298
CviAII CATG 2 cut(s) 260, 343
CviJI RGCY 6 cut(s) 43, 62, 89, 98, 157, 311
CviKI_1 RGCY 6 cut(s) 43, 62, 89, 98, 157, 311
CviQI GTAC 2 cut(s) 8, 298
DdeI CTNAG 1 cut(s) 85
DpnI GATC 1 cut(s) 223
DpnII GATC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 96
EagI CGGCCG 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 182, 344
EarI CTCTTC 2 cut(s) 182, 344
EclXI CGGCCG 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 263, 346
FaiI YATR 5 cut(s) 36, 38, 261, 287, 344
FaqI GGGAC 1 cut(s) 248
FatI CATG 2 cut(s) 259, 342
FauI CCCGC 1 cut(s) 224
FblI GTMKAC 1 cut(s) 193
Fnu4HI GCNGC 1 cut(s) 319
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 319
FspBI CTAG 1 cut(s) 349
GlaI GCGC 2 cut(s) 14, 317
GluI GCNGC 1 cut(s) 319
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 3 cut(s) 62, 98, 157
HhaI GCGC 2 cut(s) 15, 318
Hin1II CATG 2 cut(s) 263, 346
Hin6I GCGC 2 cut(s) 13, 316
HinP1I GCGC 2 cut(s) 13, 316
HincII GTYRAC 1 cut(s) 194
HindII GTYRAC 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 2 cut(s) 171, 190
HphI GGTGA 2 cut(s) 73, 155
Hpy166II GTNNAC 2 cut(s) 81, 194
Hpy188III TCNNGA 2 cut(s) 175, 293
Hpy8I GTNNAC 2 cut(s) 81, 194
HpyAV CCTTC 2 cut(s) 73, 140
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 3 cut(s) 219, 259, 321
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 237, 327
HpyF3I CTNAG 1 cut(s) 85
Hsp92II CATG 2 cut(s) 263, 346
HspAI GCGC 2 cut(s) 13, 316
Kzo9I GATC 1 cut(s) 221
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 7 cut(s) 118, 149, 171, 182, 197, 261, 288
Lsp1109I GCAGC 1 cut(s) 305
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 274
MaeI CTAG 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 100, 175
MalI GATC 1 cut(s) 223
MboI GATC 1 cut(s) 221
MboII GAAGA 2 cut(s) 199, 331
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 1 cut(s) 242
MlyI GAGTC 2 cut(s) 180, 199
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 4 cut(s) 94, 100, 228, 300
MslI CAYNNNNRTG 1 cut(s) 141
MspR9I CCNGG 1 cut(s) 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 95, 237, 327
NdeII GATC 1 cut(s) 221
NlaIII CATG 2 cut(s) 263, 346
NmuCI GTSAC 1 cut(s) 175
NspI RCATGY 1 cut(s) 263
PaeR7I CTCGAG 1 cut(s) 293
PkrI GCNGC 1 cut(s) 320
PleI GAGTC 2 cut(s) 179, 198
PpsI GAGTC 2 cut(s) 179, 198
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspPI GGNCC 1 cut(s) 155
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 9, 299
RsaNI GTAC 2 cut(s) 8, 298
RseI CAYNNNNRTG 1 cut(s) 141
SalI GTCGAC 1 cut(s) 192
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 1 cut(s) 221
Sau96I GGNCC 1 cut(s) 155
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 2 cut(s) 180, 199
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 242
SetI ASST 7 cut(s) 86, 145, 151, 239, 280, 292, 313
SexAI ACCWGGT 1 cut(s) 274
SfaNI GCATC 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SsiI CCGC 2 cut(s) 19, 231
SspMI CTAG 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 52
TaqI TCGA 2 cut(s) 193, 294
TatI WGTACW 1 cut(s) 297
TscAI CASTG 2 cut(s) 168, 261
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 318
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 192, 331
TspRI CASTG 2 cut(s) 168, 261
XceI RCATGY 1 cut(s) 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 193
XspI CTAG 1 cut(s) 349
ZrmI AGTACT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.