Rw4G001140

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011794
Physical Location & Seq
Reverse (-)
2164838 .. 2165696
859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001140.1

Sequence Viewer

Length: 333 bp
ATGGGCGTACTGCGCCACCGCCGCCACCATCACCATTCAACCCTCACAGCCTTGGCTTTCCTCTTCCTCATCACCTCAGCCTCCGCCGTACGTACTACTCCAACACAACTCGGTGGGCGACACCAGCAGCCTCAAGAGACCGAAGATGAGGTTGCGGCCGAGATGAGACTGGGAGGGCCGGGTTCTTCGCCGCCGCGATGCAGATCCAAGTGTGGAAGATGCTCGCCGTGCAAGGCGGTGCACGTGCCCATTCAGCCAGGTCTGAGCATACCTCTCGAGTATTACCCAGAAGCTTGGCGCTGCAAGTGCGGCAATAAGCTCTTCATGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

110

Amino Acids

12.18

Weight (kDa)

9.49

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 60 - 110 1.1e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22820 AT4G14723
fragaria_vesca FvH4_4g01110
malus_domestica MD13G1199400.v1.1 MD16G1199100.v1.1
prunus_persica Prupe.1G020800_v2.0.a1
pyrus_communis pycom13g17290 pycom16g16770
rosa_chinensis RchiOBHm_Chr4g0387661
rosa_laevigata RLG00000010102
rosa_multiflora Rmu_sc0003953.1_g000012
rosa_roxburghii Rroxscaffold_5G00334330
rosa_rugosa Rorug03G0312300 Rorug03G0312400
rosa_samantha Rh4AG017600 Rh4BG012400 Rh4CG018800 Rh4DG013400
rosa_wichuraiana Rw4G001140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 196
AciI CCGC 8 cut(s) 19, 22, 84, 155, 191, 194, 236, 309
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 156
AcvI CACGTG 1 cut(s) 244
AfaI GTAC 3 cut(s) 9, 90, 94
AgsI TTSAA 1 cut(s) 39
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 2 cut(s) 293, 319
AluI AGCT 2 cut(s) 293, 319
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 2 cut(s) 131, 160
Alw44I GTGCAC 1 cut(s) 239
AlwI GGATC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 2 cut(s) 156, 176
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 127, 300
AspLEI GCGC 2 cut(s) 15, 300
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 23, 64
AvaI CYCGRG 1 cut(s) 275
BaeGI GKGCMC 2 cut(s) 243, 249
BbrPI CACGTG 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 243
BbvCI CCTCAGC 1 cut(s) 76
BbvI GCAGC 2 cut(s) 139, 287
BccI CCATC 1 cut(s) 36
BceAI ACGGC 2 cut(s) 71, 211
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 258
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 131, 160
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 7 cut(s) 22, 128, 156, 191, 194, 301, 310
BlsI GCNGC 7 cut(s) 23, 129, 157, 192, 195, 302, 311
Bme1390I CCNGG 2 cut(s) 180, 258
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 176
BmrFI CCNGG 2 cut(s) 180, 258
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 188, 209
BmuI ACTGGG 1 cut(s) 179
BplI GAGNNNNNCTC 2 cut(s) 256, 288
Bpu10I CCTNAGC 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 180
BsaAI YACGTR 2 cut(s) 92, 244
BsaBI GATNNNNATC 1 cut(s) 202
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 202
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 51
BseJI GATNNNNATC 1 cut(s) 202
BseMII CTCAG 2 cut(s) 90, 254
BseNI ACTGG 1 cut(s) 174
BseSI GKGCMC 2 cut(s) 243, 249
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 2 cut(s) 139, 287
Bsh1236I CGCG 1 cut(s) 196
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 158, 178
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 179
BsiWI CGTACG 1 cut(s) 88
BsmAI GTCTC 2 cut(s) 131, 160
BsnI GGCC 2 cut(s) 158, 178
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 243, 249
Bsp143I GATC 1 cut(s) 203
BspACI CCGC 8 cut(s) 19, 22, 84, 155, 191, 194, 236, 309
BspANI GGCC 2 cut(s) 158, 178
BspCNI CTCAG 2 cut(s) 89, 255
BspFNI CGCG 1 cut(s) 196
BspPI GGATC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 258
Bst6I CTCTTC 2 cut(s) 68, 326
BstBAI YACGTR 2 cut(s) 92, 244
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 76, 263
BstFNI CGCG 1 cut(s) 196
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 2 cut(s) 15, 300
BstKTI GATC 1 cut(s) 206
BstMAI GTCTC 2 cut(s) 131, 160
BstMBI GATC 1 cut(s) 203
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 8 cut(s) 12, 21, 124, 228, 253, 306, 309, 325
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 178, 256
BstSLI GKGCMC 2 cut(s) 243, 249
BstSNI TACGTA 1 cut(s) 92
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 139, 287
BstX2I RGATCY 1 cut(s) 203
BstXI CCANNNNNNTGG 1 cut(s) 294
BstYI RGATCY 1 cut(s) 203
BstZI CGGCCG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 158, 178
BtgZI GCGATG 1 cut(s) 211
Cac8I GCNNGC 1 cut(s) 224
CfoI GCGC 2 cut(s) 15, 300
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 3 cut(s) 8, 89, 93
CviAII CATG 1 cut(s) 325
CviJI RGCY 9 cut(s) 50, 56, 80, 130, 158, 178, 256, 293, 319
CviKI_1 RGCY 9 cut(s) 50, 56, 80, 130, 158, 178, 256, 293, 319
CviQI GTAC 3 cut(s) 8, 89, 93
DdeI CTNAG 2 cut(s) 76, 263
DpnI GATC 1 cut(s) 205
DpnII GATC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 156
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 68, 326
EarI CTCTTC 2 cut(s) 68, 326
EciI GGCGGA 1 cut(s) 73
EclXI CGGCCG 1 cut(s) 156
Eco105I TACGTA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 156
Eco72I CACGTG 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 1 cut(s) 328
FaiI YATR 2 cut(s) 269, 326
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 7 cut(s) 22, 128, 156, 191, 194, 301, 310
Fsp4HI GCNGC 7 cut(s) 22, 128, 156, 191, 194, 301, 310
GlaI GCGC 2 cut(s) 14, 299
GluI GCNGC 7 cut(s) 22, 128, 156, 191, 194, 301, 310
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 2 cut(s) 158, 178
HapII CCGG 1 cut(s) 179
HhaI GCGC 2 cut(s) 15, 300
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 2 cut(s) 13, 298
HinP1I GCGC 2 cut(s) 13, 298
HindIII AAGCTT 1 cut(s) 291
HpaII CCGG 1 cut(s) 179
HphI GGTGA 2 cut(s) 23, 64
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 2 cut(s) 134, 275
Hpy8I GTNNAC 1 cut(s) 241
HpyCH4IV ACGT 2 cut(s) 91, 243
HpyCH4V TGCA 4 cut(s) 201, 231, 241, 303
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 21, 124, 228, 253, 306, 309, 325
HpyF3I CTNAG 2 cut(s) 76, 263
HpySE526I ACGT 2 cut(s) 91, 243
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 2 cut(s) 13, 298
Kzo9I GATC 1 cut(s) 203
LguI GCTCTTC 1 cut(s) 326
LpnPI CCDG 6 cut(s) 137, 155, 192, 243, 270, 300
Lsp1109I GCAGC 2 cut(s) 139, 287
LweI GCATC 2 cut(s) 188, 209
MaeII ACGT 2 cut(s) 91, 243
MalI GATC 1 cut(s) 205
MboI GATC 1 cut(s) 203
MboII GAAGA 5 cut(s) 55, 155, 177, 228, 313
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 2 cut(s) 243, 249
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 9 cut(s) 53, 71, 77, 85, 91, 141, 142, 167, 282
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 180, 258
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 8 cut(s) 12, 21, 124, 228, 253, 306, 309, 325
NciI CCSGG 1 cut(s) 180
NdeII GATC 1 cut(s) 203
NlaIII CATG 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 326
Pfl23II CGTACG 1 cut(s) 88
PkrI GCNGC 7 cut(s) 23, 129, 157, 192, 195, 302, 311
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
Ppu21I YACGTR 2 cut(s) 92, 244
Psp6I CCWGG 1 cut(s) 256
PspCI CACGTG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 256
PspLI CGTACG 1 cut(s) 88
PspPI GGNCC 1 cut(s) 176
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 3 cut(s) 9, 90, 94
RsaNI GTAC 3 cut(s) 8, 89, 93
SapI GCTCTTC 1 cut(s) 326
SatI GCNGC 7 cut(s) 22, 128, 156, 191, 194, 301, 310
Sau3AI GATC 1 cut(s) 203
Sau96I GGNCC 1 cut(s) 176
ScrFI CCNGG 2 cut(s) 180, 258
SduI GDGCHC 2 cut(s) 243, 249
SetI ASST 8 cut(s) 77, 94, 153, 246, 262, 274, 295, 321
SfaNI GCATC 2 cut(s) 188, 209
Sfr274I CTCGAG 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 132, 275
SmoI CTYRAG 2 cut(s) 132, 275
SnaBI TACGTA 1 cut(s) 92
SsiI CCGC 8 cut(s) 19, 22, 84, 155, 191, 194, 236, 309
StyD4I CCNGG 2 cut(s) 178, 256
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 2 cut(s) 94, 246
TaqI TCGA 1 cut(s) 276
TaqII GACCGA 1 cut(s) 155
TauI GCSGC 5 cut(s) 24, 158, 193, 196, 312
TseI GCWGC 2 cut(s) 127, 300
TspDTI ATGAA 1 cut(s) 313
VneI GTGCAC 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.