AT3G22950
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009607
Physical Location & Seq
Reverse (-)
8136181 .. 8138084
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22950.2

Sequence Viewer

Length: 552 bp
ATGGGAGCATTCATGTCGAGGTTTTGGTTCATGATGTTTCCTGCAAAAGAGTATAAGATTGTTGTTGTTGGTTTGGATAATGCTGGTAAAACGACGACGCTTTATAAACTTCATTTGGGTGAAGTTGTTACTACTCATCCTACTGTTGGTAGCAATGTTGAAGAGCTTGTCTACAAGAATATTCGCTTTGAGGTATGGGATCTTGGTGGGCAAGATAGGCTAAGAACTTCATGGGCAACATACTATCGTGGAACACATGCTGTGATTGTGGTTATAGATAGCACAGACAGAGCCAGAATCTCTTTCATGAAAGATGAGCTAGCCAGGTTACTTGGTCATGAGGATCTTCAAAACTCGGTCATACTAGTTTTTGCAAACAAACAGGATCTGAAAGACGCAATGACTCCTGCTGAGATCACGGATGCACTTAACCTTCACAGTATCAAGAACCATGATTGGCATATTCAAGCAAGTTGTGCGGTTACTGGAGAAGGATTGTATGATGGGCTCGGTTGGATTGCCCAAAAAGTTACCGGTAAAGCCACGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

183

Amino Acids

20.55

Weight (kDa)

6.37

Isoelectric Point (pI)

28.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 12 - 176 6.2e-67 ADP-ribosylation factor family
SRPRB PF09439 18 - 144 2e-11 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 3.7e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 133 1.7e-12 Ras family
MMR_HSR1 PF01926 19 - 124 5.9e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 479
AclWI GGATC 3 cut(s) 207, 351, 393
AfiI CCNNNNNNNGG 1 cut(s) 146
AgeI ACCGGT 1 cut(s) 533
AgsI TTSAA 3 cut(s) 161, 350, 467
AhlI ACTAGT 1 cut(s) 364
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 2 cut(s) 166, 319
AluI AGCT 2 cut(s) 166, 319
AlwI GGATC 3 cut(s) 207, 351, 393
AlwNI CAGNNNCTG 1 cut(s) 388
AsiGI ACCGGT 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 510
BauI CACGAG 1 cut(s) 544
BccI CCATC 1 cut(s) 497
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 325
BcuI ACTAGT 1 cut(s) 364
BfaI CTAG 2 cut(s) 320, 365
Bme1390I CCNGG 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 412
BmtI GCTAGC 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 533
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 2 cut(s) 160, 405
BseBI CCWGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 136, 427
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 2 cut(s) 160, 405
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 490
BshTI ACCGGT 1 cut(s) 533
BsiSI CCGG 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 146
BsmI GAATGC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 4 cut(s) 199, 343, 385, 414
BspACI CCGC 1 cut(s) 479
BspCNI CTCAG 1 cut(s) 403
BspHI TCATGA 3 cut(s) 30, 306, 337
BspOI GCTAGC 1 cut(s) 323
BspPI GGATC 3 cut(s) 207, 351, 393
BspQI GCTCTTC 1 cut(s) 156
BsrDI GCAATG 2 cut(s) 160, 405
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 533
BssMI GATC 4 cut(s) 199, 343, 385, 414
BssSI CACGAG 1 cut(s) 544
Bst2BI CACGAG 1 cut(s) 544
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 145, 440
Bst6I CTCTTC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 221, 411
BstF5I GGATG 2 cut(s) 136, 427
BstKTI GATC 4 cut(s) 202, 346, 388, 417
BstMBI GATC 4 cut(s) 199, 343, 385, 414
BstMWI GCNNNNNNNGC 2 cut(s) 217, 476
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 323
BstX2I RGATCY 3 cut(s) 199, 343, 385
BstYI RGATCY 3 cut(s) 199, 343, 385
BtsCI GGATG 2 cut(s) 136, 427
Cac8I GCNNGC 1 cut(s) 321
CaiI CAGNNNCTG 1 cut(s) 388
CciI TCATGA 3 cut(s) 30, 306, 337
Cfr10I RCCGGY 1 cut(s) 533
CseI GACGC 2 cut(s) 106, 404
CspAI ACCGGT 1 cut(s) 533
CviAII CATG 7 cut(s) 13, 31, 231, 257, 307, 338, 452
CviJI RGCY 7 cut(s) 166, 220, 293, 319, 323, 508, 542
CviKI_1 RGCY 7 cut(s) 166, 220, 293, 319, 323, 508, 542
DdeI CTNAG 2 cut(s) 221, 411
DpnI GATC 4 cut(s) 201, 345, 387, 416
DpnII GATC 4 cut(s) 199, 343, 385, 414
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 510
FaeI CATG 7 cut(s) 16, 34, 234, 260, 310, 341, 455
FatI CATG 7 cut(s) 12, 30, 230, 256, 306, 337, 451
FblI GTMKAC 1 cut(s) 171
FokI GGATG 2 cut(s) 123, 434
FriOI GRGCYC 1 cut(s) 510
FspBI CTAG 2 cut(s) 320, 365
GsuI CTGGAG 1 cut(s) 507
HapII CCGG 1 cut(s) 534
HgaI GACGC 2 cut(s) 106, 404
Hin1II CATG 7 cut(s) 16, 34, 234, 260, 310, 341, 455
HinfI GANTC 2 cut(s) 297, 403
HpaII CCGG 1 cut(s) 534
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 4 cut(s) 31, 307, 338, 445
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 2 cut(s) 97, 100
HpyAV CCTTC 2 cut(s) 443, 485
HpyCH4III ACNGT 2 cut(s) 145, 440
HpyCH4V TGCA 3 cut(s) 44, 374, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 476
HpyF3I CTNAG 2 cut(s) 221, 411
Hsp92II CATG 7 cut(s) 16, 34, 234, 260, 310, 341, 455
Kzo9I GATC 4 cut(s) 199, 343, 385, 414
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 9 cut(s) 54, 69, 307, 310, 337, 368, 420, 471, 547
LweI GCATC 1 cut(s) 412
MaeI CTAG 2 cut(s) 320, 365
MaeIII GTNAC 4 cut(s) 127, 327, 481, 529
MalI GATC 4 cut(s) 201, 345, 387, 416
MboI GATC 4 cut(s) 199, 343, 385, 414
MboII GAAGA 2 cut(s) 173, 338
MflI RGATCY 3 cut(s) 199, 343, 385
MhlI GDGCHC 1 cut(s) 510
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 3 cut(s) 12, 184, 334
MseI TTAA 2 cut(s) 429, 550
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 217, 476
NdeII GATC 4 cut(s) 199, 343, 385, 414
NheI GCTAGC 1 cut(s) 319
NlaIII CATG 7 cut(s) 16, 34, 234, 260, 310, 341, 455
NspI RCATGY 1 cut(s) 260
PagI TCATGA 3 cut(s) 30, 306, 337
PciSI GCTCTTC 1 cut(s) 156
PctI GAATGC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 297
PinAI ACCGGT 1 cut(s) 533
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
PsiI TTATAA 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PstNI CAGNNNCTG 1 cut(s) 388
PsuI RGATCY 3 cut(s) 199, 343, 385
RseI CAYNNNNRTG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 2 cut(s) 429, 550
Sau3AI GATC 4 cut(s) 199, 343, 385, 414
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 510
SetI ASST 6 cut(s) 23, 168, 195, 321, 329, 435
SfaNI GCATC 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 117
SpeI ACTAGT 1 cut(s) 364
SsiI CCGC 1 cut(s) 479
SspI AATATT 1 cut(s) 181
SspMI CTAG 2 cut(s) 320, 365
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 2 cut(s) 145, 440
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 346
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 2 cut(s) 429, 550
Tru9I TTAA 2 cut(s) 429, 550
TspDTI ATGAA 5 cut(s) 19, 101, 219, 295, 323
TspGWI ACGGA 1 cut(s) 434
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 320, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.